Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 150003965

GI number: 150003965

Start: 1872261

End: 1873595

Strand: Reverse

Name: murD [H]

Synonym: BVU_1399

Alternate gene names: 150003965

Gene position: 1873595-1872261 (Counterclockwise)

Preceding gene: 150003966

Following gene: 150003964

Centisome position: 36.29

GC content: 45.69

Gene sequence:

>1335_bases
ATGGCTAAAAGAATTGTCATACTAGGAGCCGGAGAAAGCGGTGCGGGAGCAGCCGTTCTGGCAAAGAAACAAGGTTTCGA
CACCTTCGTTTCAGACATGTCCACGATTAAGGACATATACAAAAACATGCTGAACGAGCGAGGCATCGAATGGGAAGAAG
GCAAACATACCGAATCTCTCATTCTGAATGCGGATGAAGTAATCAAAAGTCCGGGTATTCCGAATGATGCCCCGATGATT
CTTAAGTTAAAGTCACAAGGCACCCCTATCATCTCCGAAATAGAATTTGCGGGACGTTATACCAATGCCAAAATGATTTG
CATTACAGGAAGTAATGGAAAAACAACAACTACTTCGCTGATTTATCATATCTTCAAGAAAGCAGGTATGAATGTGGGAC
TAGCCGGAAATATCGGCCAAAGTCTGGCATATCAGGTAGCAGAATGCAACTATGATTATTATGTGATCGAGTTAAGCAGT
TTCCAATTGGACAACATGTACAAGTTCCATGCCAACATTGCCGTATTGATGAACATCACACCGGATCATCTGGACCGTTA
CGACCACCAGATGCAAAACTATGTGGATGCAAAATTCCGCATTATCCAGAACCAGACCCCGGACGATGCCTTCATCTTCT
GGAACGATGACCCCATCATCCAGCGTGAATTACACAAATATGGCATTCATGGCCATTACTACCCTTTTGCCGAAAGGAAA
GAGGAAGGAACCGCCGCATACGTGGAACAGGACAAGGTTTATTTTACAGAACCAATCGCTTTCAACATGGAACAGGAGGA
ACTGGCGCTGACCGGAACCCATAATCTGTTCAACTCCATGGCGGCCGGTATCTCTGCCAACCTGGCGGGTATCCGCAAAG
AGTGTATCCGCGAGGCCTTGGGTGATTTCAAGGGAGTGGAACACCGCCTTGAAAAAGTCTGCCGGGTAAGAGGGGTGGAT
TATATCAATGACTCCAAGGCTACCAATGTGAATTCCTGCTGGTATGCTTTGCAAAGCATGAAAACCAAAACCATCCTGAT
ATTAGGCGGGAAAGACAAAGGCAACGACTATAACGAAATTGCCGACTTGGTAAAAGAAAAATGTACCGGATTGATCTACA
TGGGATTGCACAATGAGAAATTGCATGATTTCTTTGACAAGTTCGGCCTGCCTGTAGCCGATGTACAAAGCATGAAGGAT
GCCGTAGACGCTGCTTACCGGATGGCCAAGAAAGGCGAAACCGTACTATTAAGTCCATGCTGCGCCAGCTTCGACCTTTT
CAAAAGTTACGAAGACCGTGGCGAGCAGTTCAAGGAATGTGTAAGAAACCTGTAA

Upstream 100 bases:

>100_bases
CGTATTCCATGAATCAAAAATCACGGTACGCTTCTGGATTGTCACCATCGTACTCGCTGCAATTACGATTATTACATTAA
AGATAAGATAAACGAAGATT

Downstream 100 bases:

>100_bases
TATAGTATTAAGGTATATAGATAATGGATCTGATAAAAGACTTGTTCAAGGGCGACAAAGTAATCTGGATTATTTTCCTG
TTTCTTTGTCTGATCTCTAT

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAKRIVILGAGESGAGAAVLAKKQGFDTFVSDMSTIKDIYKNMLNERGIEWEEGKHTESLILNADEVIKSPGIPNDAPMI
LKLKSQGTPIISEIEFAGRYTNAKMICITGSNGKTTTTSLIYHIFKKAGMNVGLAGNIGQSLAYQVAECNYDYYVIELSS
FQLDNMYKFHANIAVLMNITPDHLDRYDHQMQNYVDAKFRIIQNQTPDDAFIFWNDDPIIQRELHKYGIHGHYYPFAERK
EEGTAAYVEQDKVYFTEPIAFNMEQEELALTGTHNLFNSMAAGISANLAGIRKECIREALGDFKGVEHRLEKVCRVRGVD
YINDSKATNVNSCWYALQSMKTKTILILGGKDKGNDYNEIADLVKEKCTGLIYMGLHNEKLHDFFDKFGLPVADVQSMKD
AVDAAYRMAKKGETVLLSPCCASFDLFKSYEDRGEQFKECVRNL

Sequences:

>Translated_444_residues
MAKRIVILGAGESGAGAAVLAKKQGFDTFVSDMSTIKDIYKNMLNERGIEWEEGKHTESLILNADEVIKSPGIPNDAPMI
LKLKSQGTPIISEIEFAGRYTNAKMICITGSNGKTTTTSLIYHIFKKAGMNVGLAGNIGQSLAYQVAECNYDYYVIELSS
FQLDNMYKFHANIAVLMNITPDHLDRYDHQMQNYVDAKFRIIQNQTPDDAFIFWNDDPIIQRELHKYGIHGHYYPFAERK
EEGTAAYVEQDKVYFTEPIAFNMEQEELALTGTHNLFNSMAAGISANLAGIRKECIREALGDFKGVEHRLEKVCRVRGVD
YINDSKATNVNSCWYALQSMKTKTILILGGKDKGNDYNEIADLVKEKCTGLIYMGLHNEKLHDFFDKFGLPVADVQSMKD
AVDAAYRMAKKGETVLLSPCCASFDLFKSYEDRGEQFKECVRNL
>Mature_443_residues
AKRIVILGAGESGAGAAVLAKKQGFDTFVSDMSTIKDIYKNMLNERGIEWEEGKHTESLILNADEVIKSPGIPNDAPMIL
KLKSQGTPIISEIEFAGRYTNAKMICITGSNGKTTTTSLIYHIFKKAGMNVGLAGNIGQSLAYQVAECNYDYYVIELSSF
QLDNMYKFHANIAVLMNITPDHLDRYDHQMQNYVDAKFRIIQNQTPDDAFIFWNDDPIIQRELHKYGIHGHYYPFAERKE
EGTAAYVEQDKVYFTEPIAFNMEQEELALTGTHNLFNSMAAGISANLAGIRKECIREALGDFKGVEHRLEKVCRVRGVDY
INDSKATNVNSCWYALQSMKTKTILILGGKDKGNDYNEIADLVKEKCTGLIYMGLHNEKLHDFFDKFGLPVADVQSMKDA
VDAAYRMAKKGETVLLSPCCASFDLFKSYEDRGEQFKECVRNL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=452, Percent_Identity=32.3008849557522, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1790680, Length=337, Percent_Identity=22.5519287833828, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49955; Mature: 49824

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRIVILGAGESGAGAAVLAKKQGFDTFVSDMSTIKDIYKNMLNERGIEWEEGKHTESL
CCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
ILNADEVIKSPGIPNDAPMILKLKSQGTPIISEIEFAGRYTNAKMICITGSNGKTTTTSL
EECHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHCCEECCCEEEEEECCCCCCHHHHH
IYHIFKKAGMNVGLAGNIGQSLAYQVAECNYDYYVIELSSFQLDNMYKFHANIAVLMNIT
HHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCEEEEEECC
PDHLDRYDHQMQNYVDAKFRIIQNQTPDDAFIFWNDDPIIQRELHKYGIHGHYYPFAERK
HHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCC
EEGTAAYVEQDKVYFTEPIAFNMEQEELALTGTHNLFNSMAAGISANLAGIRKECIREAL
CCCCEEEEECCCEEEECCEEECCCHHHEEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHH
GDFKGVEHRLEKVCRVRGVDYINDSKATNVNSCWYALQSMKTKTILILGGKDKGNDYNEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHH
ADLVKEKCTGLIYMGLHNEKLHDFFDKFGLPVADVQSMKDAVDAAYRMAKKGETVLLSPC
HHHHHHHCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCC
CASFDLFKSYEDRGEQFKECVRNL
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKRIVILGAGESGAGAAVLAKKQGFDTFVSDMSTIKDIYKNMLNERGIEWEEGKHTESL
CCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
ILNADEVIKSPGIPNDAPMILKLKSQGTPIISEIEFAGRYTNAKMICITGSNGKTTTTSL
EECHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHCCEECCCEEEEEECCCCCCHHHHH
IYHIFKKAGMNVGLAGNIGQSLAYQVAECNYDYYVIELSSFQLDNMYKFHANIAVLMNIT
HHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCEEEEEECC
PDHLDRYDHQMQNYVDAKFRIIQNQTPDDAFIFWNDDPIIQRELHKYGIHGHYYPFAERK
HHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCC
EEGTAAYVEQDKVYFTEPIAFNMEQEELALTGTHNLFNSMAAGISANLAGIRKECIREAL
CCCCEEEEECCCEEEECCEEECCCHHHEEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHH
GDFKGVEHRLEKVCRVRGVDYINDSKATNVNSCWYALQSMKTKTILILGGKDKGNDYNEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHH
ADLVKEKCTGLIYMGLHNEKLHDFFDKFGLPVADVQSMKDAVDAAYRMAKKGETVLLSPC
HHHHHHHCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCC
CASFDLFKSYEDRGEQFKECVRNL
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA