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The map label for this gene is metK

Identifier: 150003753

GI number: 150003753

Start: 1599448

End: 1600740

Strand: Direct

Name: metK

Synonym: BVU_1184

Alternate gene names: 150003753

Gene position: 1599448-1600740 (Clockwise)

Preceding gene: 150003743

Following gene: 150003754

Centisome position: 30.98

GC content: 48.26

Gene sequence:

>1293_bases
ATGGGATATTTATTCACATCCGAATCGGTGTCTGAAGGACACCCCGACAAAGTGGCCGATCAAATATCGGACGCTGTGCT
TGATAAACTGTTGGCGTTTGACCCCAGTTCGAAAGTAGCTTGCGAAACCCTGGTGACTACCGGACAGGTAGTGCTGGCTG
GAGAAGTGAAAACCAAGGCGTATGTAGATTTGCAGCGCATTGCACGTGAAGTGATTAACCGTATCGGTTATACCAAAAGC
GAATATATGTTCGAAGGAAACTCCTGCGGCGTATTTTCTGCCATTCACGAACAAAGTGCGGATATCAACCGTGGCGTGGA
ACGCGAGGACCCTATGAACCAGGGTGCGGGCGACCAGGGAATGATGTTTGGTTATGCTACCAATGAAACGGAAAACTATA
TGCCGCTGTCACTGGACCTGGCACATAAACTGTTGATGGTACTGGCCGAAATCCGTCGTGAAGGGAAGGTGATGACTTAT
CTTCGTCCCGACGCCAAGAGCCAGGTTACTATTGAATACGATGATAACGGCAAGCCGGTACGCATTGATACGATTGTGGT
TTCTACCCAGCATGACGAGTTTGTAACCCCGGCTGATTCTTCCAAAGAAGCTCAGTTGAAGGCCGACGAAGAGATGTTAG
CCAAGATTCGTCAGGATGTGATTGAAATCCTGATGCCACGTGTCATTGCCGGAATTCATAATGAAGAAGTATTGGCTTTG
TTCAATGACCGGATAGTTTATCATGTGAACCCTACAGGTAAGTTTGTGATCGGCGGTCCTCATGGAGATACCGGTTTGAC
CGGCCGTAAGATTATTGTGGATACATACGGTGGAAAAGGTGCTCACGGTGGCGGGGCGTTCTCAGGAAAAGACCCCAGTA
AGGTAGACCGTAGTGCTGCCTATGCAGCACGCCATATCGCAAAGAATATGGTTGCTGCCGGTGTTGCCGATGAAATGCTG
GTGCAGGTGTCATACGCCATTGGCGTGGCCCGTCCTATCAATATCTACGTAAATACGTACGGTCGCAGCAATGTGAAACT
GAGCGACGGAGAGATTGCTAAGAAAATAGACGAACTGTTCGATCTGCGTCCGAAAGCCATTGAAGAGCGTTTGAAACTGC
GTAATCCTATTTATGAGGAAACAGCTTCTTACGGTCACATGGGACGTGAGCCGAAAGTGGTGACCAAGACATACGAATCC
ATGTATCATGAAGCAAAGACGCTGGAAGTGGAACTGTTCACTTGGGAGAAGCTGGATTATGTGGATAAGATAAAGGAAGC
GTTCGGTTTGTAA

Upstream 100 bases:

>100_bases
AATACATTGACTTTCTTATTTACATTTATCTACTCTGATAACTTCCCAAGGTGGTTAACGAATTAACTTTTAATTTTTAA
TTCTTAATTTTCAATTTAGA

Downstream 100 bases:

>100_bases
GAAACGGATTCGAATATGGTGATGTATACGGGCGAATTCAATTCGTTCCTGCGGCAATGAGCGTCATTGGCTGGCAGGGG
AGGATTGGATCCGCCCGAAA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MGYLFTSESVSEGHPDKVADQISDAVLDKLLAFDPSSKVACETLVTTGQVVLAGEVKTKAYVDLQRIAREVINRIGYTKS
EYMFEGNSCGVFSAIHEQSADINRGVEREDPMNQGAGDQGMMFGYATNETENYMPLSLDLAHKLLMVLAEIRREGKVMTY
LRPDAKSQVTIEYDDNGKPVRIDTIVVSTQHDEFVTPADSSKEAQLKADEEMLAKIRQDVIEILMPRVIAGIHNEEVLAL
FNDRIVYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDPSKVDRSAAYAARHIAKNMVAAGVADEML
VQVSYAIGVARPINIYVNTYGRSNVKLSDGEIAKKIDELFDLRPKAIEERLKLRNPIYEETASYGHMGREPKVVTKTYES
MYHEAKTLEVELFTWEKLDYVDKIKEAFGL

Sequences:

>Translated_430_residues
MGYLFTSESVSEGHPDKVADQISDAVLDKLLAFDPSSKVACETLVTTGQVVLAGEVKTKAYVDLQRIAREVINRIGYTKS
EYMFEGNSCGVFSAIHEQSADINRGVEREDPMNQGAGDQGMMFGYATNETENYMPLSLDLAHKLLMVLAEIRREGKVMTY
LRPDAKSQVTIEYDDNGKPVRIDTIVVSTQHDEFVTPADSSKEAQLKADEEMLAKIRQDVIEILMPRVIAGIHNEEVLAL
FNDRIVYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDPSKVDRSAAYAARHIAKNMVAAGVADEML
VQVSYAIGVARPINIYVNTYGRSNVKLSDGEIAKKIDELFDLRPKAIEERLKLRNPIYEETASYGHMGREPKVVTKTYES
MYHEAKTLEVELFTWEKLDYVDKIKEAFGL
>Mature_429_residues
GYLFTSESVSEGHPDKVADQISDAVLDKLLAFDPSSKVACETLVTTGQVVLAGEVKTKAYVDLQRIAREVINRIGYTKSE
YMFEGNSCGVFSAIHEQSADINRGVEREDPMNQGAGDQGMMFGYATNETENYMPLSLDLAHKLLMVLAEIRREGKVMTYL
RPDAKSQVTIEYDDNGKPVRIDTIVVSTQHDEFVTPADSSKEAQLKADEEMLAKIRQDVIEILMPRVIAGIHNEEVLALF
NDRIVYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDPSKVDRSAAYAARHIAKNMVAAGVADEMLV
QVSYAIGVARPINIYVNTYGRSNVKLSDGEIAKKIDELFDLRPKAIEERLKLRNPIYEETASYGHMGREPKVVTKTYESM
YHEAKTLEVELFTWEKLDYVDKIKEAFGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=391, Percent_Identity=51.9181585677749, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI5174529, Length=392, Percent_Identity=51.2755102040816, Blast_Score=362, Evalue=1e-100,
Organism=Escherichia coli, GI1789311, Length=428, Percent_Identity=52.5700934579439, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17538494, Length=391, Percent_Identity=52.4296675191816, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17538498, Length=390, Percent_Identity=52.3076923076923, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25145633, Length=390, Percent_Identity=52.5641025641026, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17551082, Length=391, Percent_Identity=51.150895140665, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565905, Length=391, Percent_Identity=47.8260869565217, Blast_Score=310, Evalue=7e-85,
Organism=Caenorhabditis elegans, GI32565911, Length=343, Percent_Identity=49.2711370262391, Blast_Score=294, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI25145635, Length=343, Percent_Identity=49.5626822157434, Blast_Score=294, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI32565903, Length=317, Percent_Identity=49.8422712933754, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI32565909, Length=78, Percent_Identity=64.1025641025641, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=79.4871794871795, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=390, Percent_Identity=52.0512820512821, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6320710, Length=389, Percent_Identity=50.3856041131105, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI45552159, Length=414, Percent_Identity=49.2753623188406, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24580529, Length=414, Percent_Identity=49.2753623188406, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24580537, Length=414, Percent_Identity=49.2753623188406, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24580533, Length=414, Percent_Identity=49.2753623188406, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24580535, Length=414, Percent_Identity=49.2753623188406, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24580531, Length=414, Percent_Identity=49.2753623188406, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24580527, Length=415, Percent_Identity=49.3975903614458, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24580525, Length=415, Percent_Identity=49.3975903614458, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24580541, Length=416, Percent_Identity=47.3557692307692, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24580539, Length=136, Percent_Identity=47.7941176470588, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_BACV8 (A6KZL1)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298497.1
- ProteinModelPortal:   A6KZL1
- SMR:   A6KZL1
- STRING:   A6KZL1
- GeneID:   5302150
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1184
- NMPDR:   fig|435590.6.peg.1119
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 47597; Mature: 47465

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYLFTSESVSEGHPDKVADQISDAVLDKLLAFDPSSKVACETLVTTGQVVLAGEVKTKA
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCHHH
YVDLQRIAREVINRIGYTKSEYMFEGNSCGVFSAIHEQSADINRGVEREDPMNQGAGDQG
HHHHHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC
MMFGYATNETENYMPLSLDLAHKLLMVLAEIRREGKVMTYLRPDAKSQVTIEYDDNGKPV
EEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCEE
RIDTIVVSTQHDEFVTPADSSKEAQLKADEEMLAKIRQDVIEILMPRVIAGIHNEEVLAL
EEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
FNDRIVYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDPSKVDRSAA
ECCEEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
YAARHIAKNMVAAGVADEMLVQVSYAIGVARPINIYVNTYGRSNVKLSDGEIAKKIDELF
HHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEECCCHHHHHHHHHH
DLRPKAIEERLKLRNPIYEETASYGHMGREPKVVTKTYESMYHEAKTLEVELFTWEKLDY
CCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEEEECHHHHH
VDKIKEAFGL
HHHHHHHHCC
>Mature Secondary Structure 
GYLFTSESVSEGHPDKVADQISDAVLDKLLAFDPSSKVACETLVTTGQVVLAGEVKTKA
CEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCHHH
YVDLQRIAREVINRIGYTKSEYMFEGNSCGVFSAIHEQSADINRGVEREDPMNQGAGDQG
HHHHHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC
MMFGYATNETENYMPLSLDLAHKLLMVLAEIRREGKVMTYLRPDAKSQVTIEYDDNGKPV
EEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCEE
RIDTIVVSTQHDEFVTPADSSKEAQLKADEEMLAKIRQDVIEILMPRVIAGIHNEEVLAL
EEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
FNDRIVYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDPSKVDRSAA
ECCEEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
YAARHIAKNMVAAGVADEMLVQVSYAIGVARPINIYVNTYGRSNVKLSDGEIAKKIDELF
HHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEECCCHHHHHHHHHH
DLRPKAIEERLKLRNPIYEETASYGHMGREPKVVTKTYESMYHEAKTLEVELFTWEKLDY
CCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEEEECHHHHH
VDKIKEAFGL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA