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The map label for this gene is nadB [H]

Identifier: 150003682

GI number: 150003682

Start: 1479650

End: 1481230

Strand: Direct

Name: nadB [H]

Synonym: BVU_1113

Alternate gene names: 150003682

Gene position: 1479650-1481230 (Clockwise)

Preceding gene: 150003673

Following gene: 150003683

Centisome position: 28.66

GC content: 45.67

Gene sequence:

>1581_bases
ATGGTTAAAAAATTCGATTTCCTTGTTATCGGCTCAGGACTTGCCGGAATGAGCTTCGCACTGAAAGTAGCTCACAAAGG
TAAAGTCGCCCTGATTTGTAAAGCAGGACTGGAAGAAGCCAATACCTATTTTGCTCAGGGAGGTATAGCATCTGTTACCA
ACTTGAAAGTAGACAATTTCGAGAAACACATTGAAGACACGATGATTGCCGGCGATTGGATCAGTGACCGCGCGGCTGTA
GAAAAAGTAGTGCGAGAAGCACCTGCACAAATCCAGGAACTGATAAAATGGGGAGTGGATTTTGATAAAAAAGAGGATGG
AGAATTCGACCTCCACAAAGAAGGAGGCCATTCCGAGTTCCGTATCCTGCACCACAAAGACAATACAGGAGCTGAAATAC
AAACCAGCCTGATAGAAGCGGTAAAAGCTCATCCCAATATTACCATATTTACAGATCACTATGCGGTAGAAATCATTACC
CAGCATCATTTGGGAATTATCGTCACCCGCCATACACCAGGCATCAAATGTTTCGGTGCTTACGTATTGAATGAGAAAAC
AGGAGAAGTGGATACGTTCCTGTCCAAAGTAACTGTAATGGCAACAGGCGGATGCGAAGCGGTATACCGCAATACAACCA
ATCCACTGGTAGCTACGGGAGACGGCATTGCTATGGTATACCGTGCCAAAGGAGCAGTAAAGGACATGGAATTTATACAA
TTCCATCCCACCGCCCTCTTTCATCCGGGAGACCGTCCCAGTTTCCTCATCACCGAAGCCATGCGCGGTTACGGTGCTGT
CTTAAAGAATCAAAGTGGCGAAGAATTCATGCAAAAATATGATCCACGCCTGTCATTGGCCCCACGTGATATCGTAGCAC
GTGCCATTGATAACGAAATGAAGCAGCGCGGTGAAGATCATGTATATCTGGATGTGACGCATAAAGATCCGGAAGAAACA
AAAAAACATTTCCCCAATATTTATAAAAAATGCCTCAGCCTAGGCATTGATATTACTAAGGACTATATTCCTGTGGCTCC
GGCTGCTCATTATCTGTGCGGAGGTATCAAAGTCGATCTGGACGGACAGAGCAGCATCAACCGTTTGTATGCCATCGGCG
AATGTTCATGTACCGGTCTGCACGGAGGTAACCGTCTGGCTTCCAATTCGCTGATAGAAGCAGTGGTTTATGCGGACGCG
GCAGCCAAACATGCACTAAATGTACTCGACCGGTATGACTTCAATGAGGATATCCCCGAATGGAACGATGAAGGAACCAT
GAACAATGAAGAACGGGTACTGATAACCCAAAGCATGAAAGAGGTGAACCAGATTATGGAAGCTTATGTAGGTATTGTGC
GCAGTAATACGCGATTAATCCGTGCTTGGAACCGATTGGATATTTTATATGAAGAAACCGAGCGTCTTTTCAAACGCTGT
AAGGCTTCACGCGAACTTTGCGAACTGCGTAACATGATTAACATCGGGTATTTGATCACCCGTCAGGCAATGGAACGGAA
AGAGAGTCGCGGACTGCATTATACGATTGACTATCCACATGCCGCGGCAGAGAAGAAATAA

Upstream 100 bases:

>100_bases
CTATTTCGTTTATTCTTCACGAAAAAAATAGTAAAAACAAGAGTAAAAGGTACAATGAATAAAGGTTTTTCGTAGCTTTG
CACCCATAAAGCATATTATC

Downstream 100 bases:

>100_bases
GAAACTGATTTCAGAATCCACCAAGAGGGTGTGCCAAAAGGTCTAAATAATAAACATGGCTTTTGGCACGCCCTTTTTTT
CGACCCAGTAGAAATCTAGC

Product: putative exported L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MVKKFDFLVIGSGLAGMSFALKVAHKGKVALICKAGLEEANTYFAQGGIASVTNLKVDNFEKHIEDTMIAGDWISDRAAV
EKVVREAPAQIQELIKWGVDFDKKEDGEFDLHKEGGHSEFRILHHKDNTGAEIQTSLIEAVKAHPNITIFTDHYAVEIIT
QHHLGIIVTRHTPGIKCFGAYVLNEKTGEVDTFLSKVTVMATGGCEAVYRNTTNPLVATGDGIAMVYRAKGAVKDMEFIQ
FHPTALFHPGDRPSFLITEAMRGYGAVLKNQSGEEFMQKYDPRLSLAPRDIVARAIDNEMKQRGEDHVYLDVTHKDPEET
KKHFPNIYKKCLSLGIDITKDYIPVAPAAHYLCGGIKVDLDGQSSINRLYAIGECSCTGLHGGNRLASNSLIEAVVYADA
AAKHALNVLDRYDFNEDIPEWNDEGTMNNEERVLITQSMKEVNQIMEAYVGIVRSNTRLIRAWNRLDILYEETERLFKRC
KASRELCELRNMINIGYLITRQAMERKESRGLHYTIDYPHAAAEKK

Sequences:

>Translated_526_residues
MVKKFDFLVIGSGLAGMSFALKVAHKGKVALICKAGLEEANTYFAQGGIASVTNLKVDNFEKHIEDTMIAGDWISDRAAV
EKVVREAPAQIQELIKWGVDFDKKEDGEFDLHKEGGHSEFRILHHKDNTGAEIQTSLIEAVKAHPNITIFTDHYAVEIIT
QHHLGIIVTRHTPGIKCFGAYVLNEKTGEVDTFLSKVTVMATGGCEAVYRNTTNPLVATGDGIAMVYRAKGAVKDMEFIQ
FHPTALFHPGDRPSFLITEAMRGYGAVLKNQSGEEFMQKYDPRLSLAPRDIVARAIDNEMKQRGEDHVYLDVTHKDPEET
KKHFPNIYKKCLSLGIDITKDYIPVAPAAHYLCGGIKVDLDGQSSINRLYAIGECSCTGLHGGNRLASNSLIEAVVYADA
AAKHALNVLDRYDFNEDIPEWNDEGTMNNEERVLITQSMKEVNQIMEAYVGIVRSNTRLIRAWNRLDILYEETERLFKRC
KASRELCELRNMINIGYLITRQAMERKESRGLHYTIDYPHAAAEKK
>Mature_526_residues
MVKKFDFLVIGSGLAGMSFALKVAHKGKVALICKAGLEEANTYFAQGGIASVTNLKVDNFEKHIEDTMIAGDWISDRAAV
EKVVREAPAQIQELIKWGVDFDKKEDGEFDLHKEGGHSEFRILHHKDNTGAEIQTSLIEAVKAHPNITIFTDHYAVEIIT
QHHLGIIVTRHTPGIKCFGAYVLNEKTGEVDTFLSKVTVMATGGCEAVYRNTTNPLVATGDGIAMVYRAKGAVKDMEFIQ
FHPTALFHPGDRPSFLITEAMRGYGAVLKNQSGEEFMQKYDPRLSLAPRDIVARAIDNEMKQRGEDHVYLDVTHKDPEET
KKHFPNIYKKCLSLGIDITKDYIPVAPAAHYLCGGIKVDLDGQSSINRLYAIGECSCTGLHGGNRLASNSLIEAVVYADA
AAKHALNVLDRYDFNEDIPEWNDEGTMNNEERVLITQSMKEVNQIMEAYVGIVRSNTRLIRAWNRLDILYEETERLFKRC
KASRELCELRNMINIGYLITRQAMERKESRGLHYTIDYPHAAAEKK

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=528, Percent_Identity=31.25, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI1788928, Length=521, Percent_Identity=47.2168905950096, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1790597, Length=533, Percent_Identity=32.2701688555347, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1786942, Length=572, Percent_Identity=31.6433566433566, Blast_Score=233, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17505833, Length=535, Percent_Identity=31.214953271028, Blast_Score=234, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI17550100, Length=534, Percent_Identity=30.5243445692884, Blast_Score=224, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI71986328, Length=464, Percent_Identity=27.5862068965517, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322701, Length=558, Percent_Identity=32.078853046595, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322416, Length=557, Percent_Identity=31.7773788150808, Blast_Score=234, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24663005, Length=561, Percent_Identity=32.2638146167558, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI17137288, Length=535, Percent_Identity=30.6542056074766, Blast_Score=224, Evalue=9e-59,
Organism=Drosophila melanogaster, GI24655642, Length=535, Percent_Identity=30.6542056074766, Blast_Score=224, Evalue=9e-59,
Organism=Drosophila melanogaster, GI24655647, Length=535, Percent_Identity=30.6542056074766, Blast_Score=224, Evalue=9e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 58965; Mature: 58965

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKFDFLVIGSGLAGMSFALKVAHKGKVALICKAGLEEANTYFAQGGIASVTNLKVDNF
CCCEEEEEEEECCCCCCHHEEEEECCCCEEEEEECCCHHHHHEECCCCCCEEEECCHHHH
EKHIEDTMIAGDWISDRAAVEKVVREAPAQIQELIKWGVDFDKKEDGEFDLHKEGGHSEF
HHHHHHHEECCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEE
RILHHKDNTGAEIQTSLIEAVKAHPNITIFTDHYAVEIITQHHLGIIVTRHTPGIKCFGA
EEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCEEEEEECCCCCEEEEE
YVLNEKTGEVDTFLSKVTVMATGGCEAVYRNTTNPLVATGDGIAMVYRAKGAVKDMEFIQ
EEECCCCCHHHHHHHHEEEEECCCCHHEEECCCCCEEECCCCEEEEEECCCCCCCCHHEE
FHPTALFHPGDRPSFLITEAMRGYGAVLKNQSGEEFMQKYDPRLSLAPRDIVARAIDNEM
ECCCEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
KQRGEDHVYLDVTHKDPEETKKHFPNIYKKCLSLGIDITKDYIPVAPAAHYLCGGIKVDL
HHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCEEEEE
DGQSSINRLYAIGECSCTGLHGGNRLASNSLIEAVVYADAAAKHALNVLDRYDFNEDIPE
CCCCCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
WNDEGTMNNEERVLITQSMKEVNQIMEAYVGIVRSNTRLIRAWNRLDILYEETERLFKRC
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHH
KASRELCELRNMINIGYLITRQAMERKESRGLHYTIDYPHAAAEKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MVKKFDFLVIGSGLAGMSFALKVAHKGKVALICKAGLEEANTYFAQGGIASVTNLKVDNF
CCCEEEEEEEECCCCCCHHEEEEECCCCEEEEEECCCHHHHHEECCCCCCEEEECCHHHH
EKHIEDTMIAGDWISDRAAVEKVVREAPAQIQELIKWGVDFDKKEDGEFDLHKEGGHSEF
HHHHHHHEECCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEE
RILHHKDNTGAEIQTSLIEAVKAHPNITIFTDHYAVEIITQHHLGIIVTRHTPGIKCFGA
EEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCEEEEEECCCCCEEEEE
YVLNEKTGEVDTFLSKVTVMATGGCEAVYRNTTNPLVATGDGIAMVYRAKGAVKDMEFIQ
EEECCCCCHHHHHHHHEEEEECCCCHHEEECCCCCEEECCCCEEEEEECCCCCCCCHHEE
FHPTALFHPGDRPSFLITEAMRGYGAVLKNQSGEEFMQKYDPRLSLAPRDIVARAIDNEM
ECCCEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
KQRGEDHVYLDVTHKDPEETKKHFPNIYKKCLSLGIDITKDYIPVAPAAHYLCGGIKVDL
HHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCEEEEE
DGQSSINRLYAIGECSCTGLHGGNRLASNSLIEAVVYADAAAKHALNVLDRYDFNEDIPE
CCCCCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
WNDEGTMNNEERVLITQSMKEVNQIMEAYVGIVRSNTRLIRAWNRLDILYEETERLFKRC
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHH
KASRELCELRNMINIGYLITRQAMERKESRGLHYTIDYPHAAAEKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]