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The map label for this gene is cysS

Identifier: 150003580

GI number: 150003580

Start: 1343891

End: 1345372

Strand: Direct

Name: cysS

Synonym: BVU_1009

Alternate gene names: 150003580

Gene position: 1343891-1345372 (Clockwise)

Preceding gene: 150003579

Following gene: 150003581

Centisome position: 26.03

GC content: 46.63

Gene sequence:

>1482_bases
ATGGAAAATAAACTAACCATTTACAATACATTAAGCCGTCAAAAGGAACTTTTCGTTCCCTTGCATGCCCCTCATGTAGG
TATGTATGTATGCGGTCCTACCGTATATGGCGATGCCCATTTAGGACACGCACGCCCCGCCATCACGTTCGATATCCTGT
TCCGTTATCTTACCCATCTGGGATACAAGGTACGTTATGTCCGTAACATTACCGATGTCGGTCATCTGGAACACGATGCA
GACGAAGGCGAAGATAAAATCGCCAAAAAGGCCCGTCTGGAGCAACTGGAACCCATGGAAGTAGTGCAATATTACCTCAA
TCGCTACCACAAGGCAATGGAAGCCTTGAATGTACTTCCACCCAGTATCGAGCCACATGCATCAGGCCATATCATTGAAC
AGATAGAACTGGTAGAAGAAATTCTGAAAAACGGCTATGCTTATGAAAGTGAAGGTTCCGTTTATTTCGATGTAGCAAAA
TACAACAAAGACCATCATTACGGCAAACTGTCCGGCCGCAACCTGGACGATGTGCTGAACACCACCCGCGAGCTGGACGG
TCAAAGCGAGAAGCGCAATCCTGCCGATTTCGCCCTGTGGAAATGTGCACAACCCGAACATATCATGCGCTGGCCCAGCC
CGTGGAGTAACGGATTCCCCGGTTGGCATTGTGAATGTACCGCAATGGGTAAGAAATACCTGGGCGAGCATTTCGATATT
CATGGAGGGGGAATGGACTTAATTTTCCCACACCACGAATGTGAAATCGCACAAAGCGTGGCTTCACAAGGAGATGACAT
GGTTCACTATTGGATGCACAACAACATGATTACCATTAATGGACAGAAGATGGGAAAATCATACGGCAACTTCATTAACT
TGGATGAGTTCTTCCACGGTACCCACAAGTTACTGACCCAAGCCTACAGCCCCATGACCATCCGTTTCTTCATCCTTCAG
GCACATTACCGCAGTACAGTGGACTTCAGTAACGAAGCATTACAAGCAGCCGAAAAAGGATTGGAACGGCTGACAGAAGC
TGTGAAAGGTCTTGAACGCATCACTCCGGCAACACAAACCACCGGCATAGAGGGGGTAAAAGACTTGCGTGAAAAGTGTT
ATACAGCCATGAATGATGACTTGAACTCACCGATTGTCATTGCCCATCTGTTTGACGGCGCCCGTATGATTAATACGGTT
CTGGACAAGAAAGCCACTATTTCCGCAGAAGATCTGGAAGAACTGAAAAGTATGTTCCATCTCTTTATGTACGAAATCCT
GGGTCTGAAAGAAGAAGCCGCCAATAACGAGGCACATGAAGAGGCATACGGCAAAGTAGTAGATATGCTGCTGGAACAAC
GTATGAAAGCCAAAGCCAATAAAGACTGGGCTACAAGCGATAAAATCCGTGATGAGCTGGCCGCTCTTGGCTTTGAAGTG
AAAGATACCAAAGACGGTTTCACATGGAAACTGAATAAATAG

Upstream 100 bases:

>100_bases
ATTTGTAGACAAGCAGAGGACATCAGCATCAGATTTTTTGTTAATTATTAGCCAAATACACACTTTAATTTATAACTTTG
CAATTCTAATAGAATGAATT

Downstream 100 bases:

>100_bases
AATACATAGAGCGACACTATATTTTTCACCACAGACTATACAGATTCACACAGATTACCCACCTGACAATCAATGATTTA
AGAAGCATCTGCGTTTATTT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MENKLTIYNTLSRQKELFVPLHAPHVGMYVCGPTVYGDAHLGHARPAITFDILFRYLTHLGYKVRYVRNITDVGHLEHDA
DEGEDKIAKKARLEQLEPMEVVQYYLNRYHKAMEALNVLPPSIEPHASGHIIEQIELVEEILKNGYAYESEGSVYFDVAK
YNKDHHYGKLSGRNLDDVLNTTRELDGQSEKRNPADFALWKCAQPEHIMRWPSPWSNGFPGWHCECTAMGKKYLGEHFDI
HGGGMDLIFPHHECEIAQSVASQGDDMVHYWMHNNMITINGQKMGKSYGNFINLDEFFHGTHKLLTQAYSPMTIRFFILQ
AHYRSTVDFSNEALQAAEKGLERLTEAVKGLERITPATQTTGIEGVKDLREKCYTAMNDDLNSPIVIAHLFDGARMINTV
LDKKATISAEDLEELKSMFHLFMYEILGLKEEAANNEAHEEAYGKVVDMLLEQRMKAKANKDWATSDKIRDELAALGFEV
KDTKDGFTWKLNK

Sequences:

>Translated_493_residues
MENKLTIYNTLSRQKELFVPLHAPHVGMYVCGPTVYGDAHLGHARPAITFDILFRYLTHLGYKVRYVRNITDVGHLEHDA
DEGEDKIAKKARLEQLEPMEVVQYYLNRYHKAMEALNVLPPSIEPHASGHIIEQIELVEEILKNGYAYESEGSVYFDVAK
YNKDHHYGKLSGRNLDDVLNTTRELDGQSEKRNPADFALWKCAQPEHIMRWPSPWSNGFPGWHCECTAMGKKYLGEHFDI
HGGGMDLIFPHHECEIAQSVASQGDDMVHYWMHNNMITINGQKMGKSYGNFINLDEFFHGTHKLLTQAYSPMTIRFFILQ
AHYRSTVDFSNEALQAAEKGLERLTEAVKGLERITPATQTTGIEGVKDLREKCYTAMNDDLNSPIVIAHLFDGARMINTV
LDKKATISAEDLEELKSMFHLFMYEILGLKEEAANNEAHEEAYGKVVDMLLEQRMKAKANKDWATSDKIRDELAALGFEV
KDTKDGFTWKLNK
>Mature_493_residues
MENKLTIYNTLSRQKELFVPLHAPHVGMYVCGPTVYGDAHLGHARPAITFDILFRYLTHLGYKVRYVRNITDVGHLEHDA
DEGEDKIAKKARLEQLEPMEVVQYYLNRYHKAMEALNVLPPSIEPHASGHIIEQIELVEEILKNGYAYESEGSVYFDVAK
YNKDHHYGKLSGRNLDDVLNTTRELDGQSEKRNPADFALWKCAQPEHIMRWPSPWSNGFPGWHCECTAMGKKYLGEHFDI
HGGGMDLIFPHHECEIAQSVASQGDDMVHYWMHNNMITINGQKMGKSYGNFINLDEFFHGTHKLLTQAYSPMTIRFFILQ
AHYRSTVDFSNEALQAAEKGLERLTEAVKGLERITPATQTTGIEGVKDLREKCYTAMNDDLNSPIVIAHLFDGARMINTV
LDKKATISAEDLEELKSMFHLFMYEILGLKEEAANNEAHEEAYGKVVDMLLEQRMKAKANKDWATSDKIRDELAALGFEV
KDTKDGFTWKLNK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=532, Percent_Identity=31.5789473684211, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI10835051, Length=359, Percent_Identity=33.7047353760446, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI62240992, Length=359, Percent_Identity=33.7047353760446, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI21269877, Length=359, Percent_Identity=33.7047353760446, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI303304987, Length=359, Percent_Identity=33.7047353760446, Blast_Score=188, Evalue=1e-47,
Organism=Escherichia coli, GI1786737, Length=491, Percent_Identity=41.3441955193483, Blast_Score=344, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI17509795, Length=255, Percent_Identity=36.4705882352941, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17509797, Length=255, Percent_Identity=36.4705882352941, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324082, Length=323, Percent_Identity=32.5077399380805, Blast_Score=169, Evalue=7e-43,
Organism=Drosophila melanogaster, GI19922218, Length=526, Percent_Identity=30.2281368821293, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI19922364, Length=265, Percent_Identity=36.6037735849057, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_BACV8 (A6KZ38)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298324.1
- ProteinModelPortal:   A6KZ38
- SMR:   A6KZ38
- STRING:   A6KZ38
- GeneID:   5301976
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1009
- NMPDR:   fig|435590.6.peg.949
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 56317; Mature: 56317

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 286-286

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKLTIYNTLSRQKELFVPLHAPHVGMYVCGPTVYGDAHLGHARPAITFDILFRYLTHL
CCCCEEEHHHHHCCCCEEEEECCCCCCEEEECCEEECCCCCCCCCCHHHHHHHHHHHHHC
GYKVRYVRNITDVGHLEHDADEGEDKIAKKARLEQLEPMEVVQYYLNRYHKAMEALNVLP
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
PSIEPHASGHIIEQIELVEEILKNGYAYESEGSVYFDVAKYNKDHHYGKLSGRNLDDVLN
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
TTRELDGQSEKRNPADFALWKCAQPEHIMRWPSPWSNGFPGWHCECTAMGKKYLGEHFDI
HHHHCCCCCCCCCCCHHHHEECCCCHHHCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCEE
HGGGMDLIFPHHECEIAQSVASQGDDMVHYWMHNNMITINGQKMGKSYGNFINLDEFFHG
CCCCEEEECCCHHHHHHHHHHHCCCCEEEEEEECCEEEECCHHHHHHHCCEECHHHHHHH
THKLLTQAYSPMTIRFFILQAHYRSTVDFSNEALQAAEKGLERLTEAVKGLERITPATQT
HHHHHHHHCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHC
TGIEGVKDLREKCYTAMNDDLNSPIVIAHLFDGARMINTVLDKKATISAEDLEELKSMFH
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
LFMYEILGLKEEAANNEAHEEAYGKVVDMLLEQRMKAKANKDWATSDKIRDELAALGFEV
HHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCE
KDTKDGFTWKLNK
EECCCCCEEEECC
>Mature Secondary Structure
MENKLTIYNTLSRQKELFVPLHAPHVGMYVCGPTVYGDAHLGHARPAITFDILFRYLTHL
CCCCEEEHHHHHCCCCEEEEECCCCCCEEEECCEEECCCCCCCCCCHHHHHHHHHHHHHC
GYKVRYVRNITDVGHLEHDADEGEDKIAKKARLEQLEPMEVVQYYLNRYHKAMEALNVLP
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
PSIEPHASGHIIEQIELVEEILKNGYAYESEGSVYFDVAKYNKDHHYGKLSGRNLDDVLN
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
TTRELDGQSEKRNPADFALWKCAQPEHIMRWPSPWSNGFPGWHCECTAMGKKYLGEHFDI
HHHHCCCCCCCCCCCHHHHEECCCCHHHCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCEE
HGGGMDLIFPHHECEIAQSVASQGDDMVHYWMHNNMITINGQKMGKSYGNFINLDEFFHG
CCCCEEEECCCHHHHHHHHHHHCCCCEEEEEEECCEEEECCHHHHHHHCCEECHHHHHHH
THKLLTQAYSPMTIRFFILQAHYRSTVDFSNEALQAAEKGLERLTEAVKGLERITPATQT
HHHHHHHHCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHC
TGIEGVKDLREKCYTAMNDDLNSPIVIAHLFDGARMINTVLDKKATISAEDLEELKSMFH
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
LFMYEILGLKEEAANNEAHEEAYGKVVDMLLEQRMKAKANKDWATSDKIRDELAALGFEV
HHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCE
KDTKDGFTWKLNK
EECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA