Click here to switch to the map view.

The map label for this gene is cca [H]

Identifier: 148828409

GI number: 148828409

Start: 1862921

End: 1864171

Strand: Reverse

Name: cca [H]

Synonym: CGSHiGG_10090

Alternate gene names: 148828409

Gene position: 1864171-1862921 (Counterclockwise)

Preceding gene: 148828410

Following gene: 148828408

Centisome position: 98.78

GC content: 39.01

Gene sequence:

>1251_bases
ATGAAAATTTATTTAGTTGGTGGGGCTGTTCGTGATCAATTATTGGGGTTACCAGTAAAAGATCGTGATTGGATTGTGGT
AGGCGCAGACCCCGCTACGCTACTCTCTCTCGGTTATCAGCAAGTAGGCAAAGATTTTCCAGTTTTTCTCAATCCTAAAA
CAAAAGAAGAATATGCGCTTGCCCGAACAGAACGTAAATCTAGCACAGGTTACACGGGCTTTATTTGTGATTTCTCCCCA
ACAATCACATTAGAACAAGATTTAATTCGACGAGATCTCACTATTAACGCCATGGCTCAAAGCGAAGACGGTGAAATTAT
CGATCCTTATGGTGGAAAACAAGATTTAGAAAATCGAATTTTGCGCCATATTTCTCCCGCTTTTTCAGAAGATCCTTTAC
GAGTATTGCGCGTGGCACGCTTTGCAGCCCGTTATCATTCTCTCGGTTTTAAAATCGCCTCGGAAACATTAGCTCTAATG
GCAGAACTTGCCCAATCGGGAGAATTACAACATCTCACGGCGGAACGCGTTTGGCTAGAAACAGAAAAAGCCTTAAACGA
AAAAAATCCTGAAATTTATTTTGAAACCTTACATAAAACAGGCGCATTAAGCGTTTTATTTTCTGAAATCGATGCCCTTC
ATGGGGTGCCAAATCCAGTAAAACATCATCCCGAAGTGGATAGTTTTATTCATACCATGTTGGTATTAAAACAAGCGGTT
AATTTGACTGAAAATAACCCTATCCTAAATAAAAGTGCGGTTCGTTTTGCCGCAATTTGTCACGATCTTGGCAAGGCTCT
TACACCTCAAAATATTCTTCCTCATCATTATGGGCATGAACAAGCTGGCATAAAACCGACTAGATCATTGTGCAAACGCT
TGAAAGTCCCAAGTTATTTTCAAGAACTTGCAGAACTGACTTGTGAATTTCACACACATATTCACAAAGCCTTTGAGCTT
CGAGCTGAAACCGTCATAACGCTGTTTAATCGTTTTGATGTGTGGAGAAAATCACAACGCTTTCAAGAATTTCTACAAGT
ATGTCTAGCTGATACTCGAGGTAGAACAGGTTTTGAAAATAAAGACTATCCACAAATAGATTACATCAATCAATTATTGC
ATGCGGCAAATGAAGTAGATGTACAGCAAGTTATTGCTGATGGATTTGAGAAACAAGCAATCAAAAATGAATTGACGAAA
CGAAGAATCTTAGCGGTTAAACAAACAAAAACGAATTACCCAACAAATTAA

Upstream 100 bases:

>100_bases
TTATGGCGGTTCAGTATTAGGCGTAGGCTTACTCTTCGGCTTGCTCATTCCTTATGTGTTACCAAAACGTCGCCGTCGTG
ATGGTTGGGCATAATCACGA

Downstream 100 bases:

>100_bases
CAAATAAAAGTAACTTTACTCAATTAAACTAAATCCTTAAAATATGCGAGCTTATTAACTTGATGAATAATATGAAAACC
TTTAAATTTTTCACCGCACT

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKIYLVGGAVRDQLLGLPVKDRDWIVVGADPATLLSLGYQQVGKDFPVFLNPKTKEEYALARTERKSSTGYTGFICDFSP
TITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRILRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALM
AELAQSGELQHLTAERVWLETEKALNEKNPEIYFETLHKTGALSVLFSEIDALHGVPNPVKHHPEVDSFIHTMLVLKQAV
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYFQELAELTCEFHTHIHKAFEL
RAETVITLFNRFDVWRKSQRFQEFLQVCLADTRGRTGFENKDYPQIDYINQLLHAANEVDVQQVIADGFEKQAIKNELTK
RRILAVKQTKTNYPTN

Sequences:

>Translated_416_residues
MKIYLVGGAVRDQLLGLPVKDRDWIVVGADPATLLSLGYQQVGKDFPVFLNPKTKEEYALARTERKSSTGYTGFICDFSP
TITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRILRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALM
AELAQSGELQHLTAERVWLETEKALNEKNPEIYFETLHKTGALSVLFSEIDALHGVPNPVKHHPEVDSFIHTMLVLKQAV
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYFQELAELTCEFHTHIHKAFEL
RAETVITLFNRFDVWRKSQRFQEFLQVCLADTRGRTGFENKDYPQIDYINQLLHAANEVDVQQVIADGFEKQAIKNELTK
RRILAVKQTKTNYPTN
>Mature_416_residues
MKIYLVGGAVRDQLLGLPVKDRDWIVVGADPATLLSLGYQQVGKDFPVFLNPKTKEEYALARTERKSSTGYTGFICDFSP
TITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRILRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALM
AELAQSGELQHLTAERVWLETEKALNEKNPEIYFETLHKTGALSVLFSEIDALHGVPNPVKHHPEVDSFIHTMLVLKQAV
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYFQELAELTCEFHTHIHKAFEL
RAETVITLFNRFDVWRKSQRFQEFLQVCLADTRGRTGFENKDYPQIDYINQLLHAANEVDVQQVIADGFEKQAIKNELTK
RRILAVKQTKTNYPTN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=195, Percent_Identity=31.2820512820513, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1789436, Length=410, Percent_Identity=58.5365853658537, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI87081691, Length=177, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321016, Length=238, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281360112, Length=246, Percent_Identity=27.6422764227642, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21357337, Length=246, Percent_Identity=27.6422764227642, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47255; Mature: 47255

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAVRDQLLGLPVKDRDWIVVGADPATLLSLGYQQVGKDFPVFLNPKTKEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKSSTGYTGFICDFSPTITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRI
HHHHCCCCCCCEEEEECCCCCEEEHHHHHHHCCEEEEEECCCCCCEECCCCCHHHHHHHH
LRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALMAELAQSGELQHLTAERVWLE
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TEKALNEKNPEIYFETLHKTGALSVLFSEIDALHGVPNPVKHHPEVDSFIHTMLVLKQAV
HHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHH
QELAELTCEFHTHIHKAFELRAETVITLFNRFDVWRKSQRFQEFLQVCLADTRGRTGFEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KDYPQIDYINQLLHAANEVDVQQVIADGFEKQAIKNELTKRRILAVKQTKTNYPTN
CCCCHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIYLVGGAVRDQLLGLPVKDRDWIVVGADPATLLSLGYQQVGKDFPVFLNPKTKEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKSSTGYTGFICDFSPTITLEQDLIRRDLTINAMAQSEDGEIIDPYGGKQDLENRI
HHHHCCCCCCCEEEEECCCCCEEEHHHHHHHCCEEEEEECCCCCCEECCCCCHHHHHHHH
LRHISPAFSEDPLRVLRVARFAARYHSLGFKIASETLALMAELAQSGELQHLTAERVWLE
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TEKALNEKNPEIYFETLHKTGALSVLFSEIDALHGVPNPVKHHPEVDSFIHTMLVLKQAV
HHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
NLTENNPILNKSAVRFAAICHDLGKALTPQNILPHHYGHEQAGIKPTRSLCKRLKVPSYF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHH
QELAELTCEFHTHIHKAFELRAETVITLFNRFDVWRKSQRFQEFLQVCLADTRGRTGFEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KDYPQIDYINQLLHAANEVDVQQVIADGFEKQAIKNELTKRRILAVKQTKTNYPTN
CCCCHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA