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The map label for this gene is mdh [H]

Identifier: 148828359

GI number: 148828359

Start: 1804854

End: 1805789

Strand: Direct

Name: mdh [H]

Synonym: CGSHiGG_09775

Alternate gene names: 148828359

Gene position: 1804854-1805789 (Clockwise)

Preceding gene: 148828356

Following gene: 148828362

Centisome position: 95.64

GC content: 40.81

Gene sequence:

>936_bases
ATGAAAGTTGCTGTATTAGGTGCCGCAGGTGGTATTGGTCAAGCATTAGCGTTATTACTAAAACTTCAGTTGCCAGCAGG
CACCGACTTAGCATTATATGATATTGCCCCAGTAACACCAGGTGTTGCAGTAGATGTGAGCCATATTCCAACGGCAGTGA
ATGTGAAAGGTTTTTCTGGTGAAGATCCAACACCCGCACTTGAAGGTGCGGATGTTGTATTAATTTCTGCTGGTGTTGCG
CGTAAACCTGGTATGGATCGTTCAGATTTATTCAATATTAATGCAGGTATCGTGCGTGGTTTAATTGAGAAAGTCGCGAT
TACTTGCCCAAAAGCTTGTGTTGGTATCATCACTAACCCAGTAAATACTACTGTTGCGATTGCGGCTGAAGTGTTGAAAA
AAGCAGGTGTTTACGACAAACGTAAATTATTTGGTGTGACAACTTTAGACGTGTTACGTTCTGAAACTTTTGTGGCTGAA
TTAAAAGGTTTAAATGTTTCTCGTACGAGCGTTCCTGTTATTGGTGGTCACTCAGGTGTGACTATTCTTCCATTACTTTC
TCAAGTTCAATATGCAAAATGGAACGAAGAAGAAATCGAACCATTAACAAAACGTATCCAAAATGCAGGTACAGAAGTGG
TCAATGCAAAAGCAGGTGGCGGTTCTGCAACGCTTTCAATGGCGCAAGCTGCAGCACGTTTTGCGCGTTCTTTAGTGAAA
GGATTAAGTGGCGAGACAGTGGTTGAATGTACTTATGTTGAAGGCGATGGCAAATATGCTCGTTTCTTCTCTCAACCCGT
TCGTTTAGGCAAAGAGGGTGTAGAAGAAATTTTACCAATTGGCCCATTAAGCAATTTTGAACAACAAGCTTTAGAAAATA
TGTTGCCAACTTTACGTGCAGATATTGAATTAGGCGAAAAATTTATTAATGGTTAA

Upstream 100 bases:

>100_bases
TGTGAACTAGATCATAGAATGATATTTCTCTATTTGTAATTGTAAGATGAACATTTTAAAATCAGGCTGTTTTAAATTAA
CTAACACATAAGGAGTATTT

Downstream 100 bases:

>100_bases
TCGTTAAACAATAAGAAGGGAATATAAAATTCTCTTTTTATTAAAATAAAAAAGCCACCAAATGGTGGCTTTTGTTTGTA
TCTTTATTATTTCTGACGCA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVAVLGAAGGIGQALALLLKLQLPAGTDLALYDIAPVTPGVAVDVSHIPTAVNVKGFSGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRGLIEKVAITCPKACVGIITNPVNTTVAIAAEVLKKAGVYDKRKLFGVTTLDVLRSETFVAE
LKGLNVSRTSVPVIGGHSGVTILPLLSQVQYAKWNEEEIEPLTKRIQNAGTEVVNAKAGGGSATLSMAQAAARFARSLVK
GLSGETVVECTYVEGDGKYARFFSQPVRLGKEGVEEILPIGPLSNFEQQALENMLPTLRADIELGEKFING

Sequences:

>Translated_311_residues
MKVAVLGAAGGIGQALALLLKLQLPAGTDLALYDIAPVTPGVAVDVSHIPTAVNVKGFSGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRGLIEKVAITCPKACVGIITNPVNTTVAIAAEVLKKAGVYDKRKLFGVTTLDVLRSETFVAE
LKGLNVSRTSVPVIGGHSGVTILPLLSQVQYAKWNEEEIEPLTKRIQNAGTEVVNAKAGGGSATLSMAQAAARFARSLVK
GLSGETVVECTYVEGDGKYARFFSQPVRLGKEGVEEILPIGPLSNFEQQALENMLPTLRADIELGEKFING
>Mature_311_residues
MKVAVLGAAGGIGQALALLLKLQLPAGTDLALYDIAPVTPGVAVDVSHIPTAVNVKGFSGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVRGLIEKVAITCPKACVGIITNPVNTTVAIAAEVLKKAGVYDKRKLFGVTTLDVLRSETFVAE
LKGLNVSRTSVPVIGGHSGVTILPLLSQVQYAKWNEEEIEPLTKRIQNAGTEVVNAKAGGGSATLSMAQAAARFARSLVK
GLSGETVVECTYVEGDGKYARFFSQPVRLGKEGVEEILPIGPLSNFEQQALENMLPTLRADIELGEKFING

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=311, Percent_Identity=55.6270096463022, Blast_Score=318, Evalue=3e-87,
Organism=Escherichia coli, GI1789632, Length=311, Percent_Identity=79.0996784565916, Blast_Score=488, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=59.1054313099042, Blast_Score=342, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=50.1587301587302, Blast_Score=272, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6320125, Length=338, Percent_Identity=40.2366863905325, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6324446, Length=278, Percent_Identity=43.5251798561151, Blast_Score=182, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24647881, Length=312, Percent_Identity=58.974358974359, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24663599, Length=316, Percent_Identity=49.6835443037975, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24663595, Length=298, Percent_Identity=45.3020134228188, Blast_Score=254, Evalue=6e-68,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32541; Mature: 32541

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVLGAAGGIGQALALLLKLQLPAGTDLALYDIAPVTPGVAVDVSHIPTAVNVKGFSG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHCCCEEEECCCCC
EDPTPALEGADVVLISAGVARKPGMDRSDLFNINAGIVRGLIEKVAITCPKACVGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECHHHHHHHHHHHHHHHCCHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKRKLFGVTTLDVLRSETFVAELKGLNVSRTSVPVIGGHSGV
CHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCC
TILPLLSQVQYAKWNEEEIEPLTKRIQNAGTEVVNAKAGGGSATLSMAQAAARFARSLVK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GLSGETVVECTYVEGDGKYARFFSQPVRLGKEGVEEILPIGPLSNFEQQALENMLPTLRA
CCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
DIELGEKFING
HHHHHHHHHCC
>Mature Secondary Structure
MKVAVLGAAGGIGQALALLLKLQLPAGTDLALYDIAPVTPGVAVDVSHIPTAVNVKGFSG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHCCCEEEECCCCC
EDPTPALEGADVVLISAGVARKPGMDRSDLFNINAGIVRGLIEKVAITCPKACVGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECHHHHHHHHHHHHHHHCCHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKRKLFGVTTLDVLRSETFVAELKGLNVSRTSVPVIGGHSGV
CHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCC
TILPLLSQVQYAKWNEEEIEPLTKRIQNAGTEVVNAKAGGGSATLSMAQAAARFARSLVK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GLSGETVVECTYVEGDGKYARFFSQPVRLGKEGVEEILPIGPLSNFEQQALENMLPTLRA
CCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
DIELGEKFING
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA