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The map label for this gene is purF [H]

Identifier: 148828356

GI number: 148828356

Start: 1801730

End: 1803247

Strand: Direct

Name: purF [H]

Synonym: CGSHiGG_09760

Alternate gene names: 148828356

Gene position: 1801730-1803247 (Clockwise)

Preceding gene: 148828355

Following gene: 148828359

Centisome position: 95.47

GC content: 41.24

Gene sequence:

>1518_bases
ATGTGTGGTATTGTCGGTATTGTTAGCCAAAGTCCGGTTAATGAATCTATTTATGCAGCATTAACCCTTTTGCAACATCG
TGGACAAGATGCGGCAGGTATTGTAACGGTTGATGATGAAAACCGTTTCCGCTTACGTAAAGCTAATGGTTTAGTCAGCG
ATGTGTTTCATCAAGAACATATGCTACGCTTGCAAGGACATGCTGGGTTGGGGCATGTTCGCTATCCTACAGCGGGGAGT
TCTAGCGTCTCAGAGGCTCAGCCTTTTTATGTGAATTCTCCCTATGGTGTCACTTTGGTGCATAACGGCAACTTAACCAA
CTCAGTGGAGTTAAAAGAAAAGGTCTTTAAAACTGCTCGCCGCCATGTAAACACTAATTCAGATTCCGAACTTCTTCTTA
ATATTCTCGCCAATCACCTCGATCACATTCCTCAAGATCACCTCGATCCACAAGATATTTTCTATGCCGTTCGTAAAACT
CATAAGGATGTTCGTGGTGCTTATGCTTGTTTAGCGATGATTATTGGACATGGCATGGTTGCGTTTCGTGATCCTTTTGG
CATTCGTCCTTTGGTGTTGGGTAAACGCGAGGAAAATGGCAAAACCGATTATATGTTTGCCTCTGAAACAGTGGCTTTAG
ATATTGTAGGTTTCGAATTTGTGCGTGATATTGCTGCAGGTGAAGCCGTTTATGTGACGTTTGATGGCGAGCTTTATTCA
CAACAATGTGCTGAAAGTGCGGTGTTAAATCCTTGTATTTTTGAGTATGTTTATTTTGCACGCCCAGATTCAACGATTGA
TGGCGTCTCTGTTTATGCTGCGCGTGTTCACATGGGCGAAAAACTTGGACAAAAAATTGCAAAAGAATGGGCTGATGAAA
TTGATAATATTGATGTGGTGATTCCCGTTCCTGAAACTTCAACAGATATTGCGTTACAAATAGCTCGTGTACTAGGGAAG
CCTTATCGCCAAGGATTTGTGAAAAATCGCTATGTTGGCCGTACCTTTATTATGCCAGGACAAGCACAGCGTATTAGTTC
GGTTCGTCGTAAATTGAATACCATTAAAGCTGAATTTAAAGATAAAAACGTGTTATTGGTAGACGATTCTATTGTTCGTG
GCACAACCTCTGAGCAAATTGTTGAAATGGCTCGCTCAGCTGGCGCGAAGAAAATTTACTTCGCCTCGGCTGCCCCAGAA
ATTCGTTATCCGAATGTCTATGGTATTGATATGCCGAGTCGCGATGAGCTGATCGCTTATGGACGAAATGTTGATGAAAT
TGCACATTTGATTGGCGTAGATAAATTAATTTTCCAAGATCTGACCGCACTTACTGAATCTGTTCAATTGGAAAACCCAG
CCATTCAAGGTTTTGATTGTTCTGTTTTCACAGGCGAATATATTACAGGCGATATTAGCCCTGAATATTTAGAGAAAATC
GCAACTCAACGCAATGATAACGCAAAGAAGAAACGAGAAAAACAAGCATCCAATCTTGAAATTTACAATGAACAATAA

Upstream 100 bases:

>100_bases
TTAATACCGCACTTTGGTTTTATTATTGAATGGTTCTTTCAACAACTTCAGGCGAGTTCAAGTTTTTTAACTCCAACGCT
TAATCAATAAGGAACACAAA

Downstream 100 bases:

>100_bases
ATGCTAGGCATTTAGAGGGATAAAAATCATCGTATTAAAAGTGCGGTGATTTTTTTATTTGTTTTTTAGGATATCTTTTA
GATAGTTTTCGCAATCAGCA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MCGIVGIVSQSPVNESIYAALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFHQEHMLRLQGHAGLGHVRYPTAGS
SSVSEAQPFYVNSPYGVTLVHNGNLTNSVELKEKVFKTARRHVNTNSDSELLLNILANHLDHIPQDHLDPQDIFYAVRKT
HKDVRGAYACLAMIIGHGMVAFRDPFGIRPLVLGKREENGKTDYMFASETVALDIVGFEFVRDIAAGEAVYVTFDGELYS
QQCAESAVLNPCIFEYVYFARPDSTIDGVSVYAARVHMGEKLGQKIAKEWADEIDNIDVVIPVPETSTDIALQIARVLGK
PYRQGFVKNRYVGRTFIMPGQAQRISSVRRKLNTIKAEFKDKNVLLVDDSIVRGTTSEQIVEMARSAGAKKIYFASAAPE
IRYPNVYGIDMPSRDELIAYGRNVDEIAHLIGVDKLIFQDLTALTESVQLENPAIQGFDCSVFTGEYITGDISPEYLEKI
ATQRNDNAKKKREKQASNLEIYNEQ

Sequences:

>Translated_505_residues
MCGIVGIVSQSPVNESIYAALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFHQEHMLRLQGHAGLGHVRYPTAGS
SSVSEAQPFYVNSPYGVTLVHNGNLTNSVELKEKVFKTARRHVNTNSDSELLLNILANHLDHIPQDHLDPQDIFYAVRKT
HKDVRGAYACLAMIIGHGMVAFRDPFGIRPLVLGKREENGKTDYMFASETVALDIVGFEFVRDIAAGEAVYVTFDGELYS
QQCAESAVLNPCIFEYVYFARPDSTIDGVSVYAARVHMGEKLGQKIAKEWADEIDNIDVVIPVPETSTDIALQIARVLGK
PYRQGFVKNRYVGRTFIMPGQAQRISSVRRKLNTIKAEFKDKNVLLVDDSIVRGTTSEQIVEMARSAGAKKIYFASAAPE
IRYPNVYGIDMPSRDELIAYGRNVDEIAHLIGVDKLIFQDLTALTESVQLENPAIQGFDCSVFTGEYITGDISPEYLEKI
ATQRNDNAKKKREKQASNLEIYNEQ
>Mature_505_residues
MCGIVGIVSQSPVNESIYAALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFHQEHMLRLQGHAGLGHVRYPTAGS
SSVSEAQPFYVNSPYGVTLVHNGNLTNSVELKEKVFKTARRHVNTNSDSELLLNILANHLDHIPQDHLDPQDIFYAVRKT
HKDVRGAYACLAMIIGHGMVAFRDPFGIRPLVLGKREENGKTDYMFASETVALDIVGFEFVRDIAAGEAVYVTFDGELYS
QQCAESAVLNPCIFEYVYFARPDSTIDGVSVYAARVHMGEKLGQKIAKEWADEIDNIDVVIPVPETSTDIALQIARVLGK
PYRQGFVKNRYVGRTFIMPGQAQRISSVRRKLNTIKAEFKDKNVLLVDDSIVRGTTSEQIVEMARSAGAKKIYFASAAPE
IRYPNVYGIDMPSRDELIAYGRNVDEIAHLIGVDKLIFQDLTALTESVQLENPAIQGFDCSVFTGEYITGDISPEYLEKI
ATQRNDNAKKKREKQASNLEIYNEQ

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=477, Percent_Identity=34.8008385744235, Blast_Score=259, Evalue=4e-69,
Organism=Escherichia coli, GI1788651, Length=506, Percent_Identity=68.9723320158103, Blast_Score=729, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=224, Percent_Identity=29.4642857142857, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=33.1262939958592, Blast_Score=223, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6323958, Length=511, Percent_Identity=49.3150684931507, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24659598, Length=496, Percent_Identity=33.2661290322581, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24659604, Length=475, Percent_Identity=34.1052631578947, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI28573187, Length=499, Percent_Identity=33.2665330661323, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56237; Mature: 56237

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVSQSPVNESIYAALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFHQEH
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEHHCCHHHHHHHHHH
MLRLQGHAGLGHVRYPTAGSSSVSEAQPFYVNSPYGVTLVHNGNLTNSVELKEKVFKTAR
HHEEECCCCCCEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
RHVNTNSDSELLLNILANHLDHIPQDHLDPQDIFYAVRKTHKDVRGAYACLAMIIGHGMV
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AFRDPFGIRPLVLGKREENGKTDYMFASETVALDIVGFEFVRDIAAGEAVYVTFDGELYS
EECCCCCCCCEEECCCCCCCCCCEEEECCEEEEEEHHHHHHHHHHCCCEEEEEECCHHHH
QQCAESAVLNPCIFEYVYFARPDSTIDGVSVYAARVHMGEKLGQKIAKEWADEIDNIDVV
HHHHHHHHCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IPVPETSTDIALQIARVLGKPYRQGFVKNRYVGRTFIMPGQAQRISSVRRKLNTIKAEFK
EECCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
DKNVLLVDDSIVRGTTSEQIVEMARSAGAKKIYFASAAPEIRYPNVYGIDMPSRDELIAY
CCCEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCHHCC
GRNVDEIAHLIGVDKLIFQDLTALTESVQLENPAIQGFDCSVFTGEYITGDISPEYLEKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEECCCCHHHHHHH
ATQRNDNAKKKREKQASNLEIYNEQ
HHCCCCCHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MCGIVGIVSQSPVNESIYAALTLLQHRGQDAAGIVTVDDENRFRLRKANGLVSDVFHQEH
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEHHCCHHHHHHHHHH
MLRLQGHAGLGHVRYPTAGSSSVSEAQPFYVNSPYGVTLVHNGNLTNSVELKEKVFKTAR
HHEEECCCCCCEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
RHVNTNSDSELLLNILANHLDHIPQDHLDPQDIFYAVRKTHKDVRGAYACLAMIIGHGMV
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AFRDPFGIRPLVLGKREENGKTDYMFASETVALDIVGFEFVRDIAAGEAVYVTFDGELYS
EECCCCCCCCEEECCCCCCCCCCEEEECCEEEEEEHHHHHHHHHHCCCEEEEEECCHHHH
QQCAESAVLNPCIFEYVYFARPDSTIDGVSVYAARVHMGEKLGQKIAKEWADEIDNIDVV
HHHHHHHHCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IPVPETSTDIALQIARVLGKPYRQGFVKNRYVGRTFIMPGQAQRISSVRRKLNTIKAEFK
EECCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
DKNVLLVDDSIVRGTTSEQIVEMARSAGAKKIYFASAAPEIRYPNVYGIDMPSRDELIAY
CCCEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCHHCC
GRNVDEIAHLIGVDKLIFQDLTALTESVQLENPAIQGFDCSVFTGEYITGDISPEYLEKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEECCCCHHHHHHH
ATQRNDNAKKKREKQASNLEIYNEQ
HHCCCCCHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]