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The map label for this gene is ligA [H]

Identifier: 148828335

GI number: 148828335

Start: 1777363

End: 1778058

Strand: Direct

Name: ligA [H]

Synonym: CGSHiGG_09605

Alternate gene names: 148828335

Gene position: 1777363-1778058 (Clockwise)

Preceding gene: 148828328

Following gene: 148828342

Centisome position: 94.18

GC content: 37.21

Gene sequence:

>696_bases
ATGTCTGAAAAATTAGATGGCGTACGCGGTTATTGGGATGGAAAGCAATTATTAACCCGACAAGGGCAACCTTTATCGCC
GCCCGCTTATTTCATTAAGGATTTTCCGCCTTTTGCGATTGATGGCGAATTATTTAGTGAGCGAAATCATTTTGAGGAAA
TTTCATCCATCACAAAATCTTTTAAAGGCGATGGTTGGGCAAAACTTAAATTGTATGTTTTTGATGTGCCTGATGCAGAG
GGCAATTTATTTGAGCGTTTAGCTAAATTAAAAGCGCATTTACTTGAACATCCAACAACTTACATTGAAATTATTGAGCA
AATTCCTGTTAAAGATAAAACCCATTTATATCAATTTCTTGCACAAGTCGAAAATTTGCAAGGCGAGGGCGTTGTTGTAC
GTAACCCTAATGCACCTTATGAACGTAAGCGAAGCTCACAAATTTTAAAATTGAAAACAGCACGAGATGAAGAATGTACG
GTTATTGCACACCATAAAGGCAAAGGACAATTTGAAAATGTGATGGGTGCTTTAACTTGTAAAAATCATCGGGGAGAATT
TAAAATCGGTTCGGGTTTTAATTTAAACGAACGAGAAAATCCCCCTCCGATAGGATCTGTGATTACTTATAAATATCGAG
GCATTACGAATAGTGGAAAACCTCGATTTGCAACCTATTGGCGAGAGAAAAAATGA

Upstream 100 bases:

>100_bases
CCGCACTTTGTTACTTTTCTTTGCTAGTTCTTTTGCTTTTGCTAACTCAGATTTAATGCTTCTTCATACCTACAATAATC
AACCCATAGAGGGTTGGGTA

Downstream 100 bases:

>100_bases
GCCTATTTATAAATCTGACAAAATGAATTTAATCAATATGGAAACAAGCGATTAATTTTCCATCAGTATATTGTTCCAAG
TGCGGTTGATTTTCACGACA

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSEKLDGVRGYWDGKQLLTRQGQPLSPPAYFIKDFPPFAIDGELFSERNHFEEISSITKSFKGDGWAKLKLYVFDVPDAE
GNLFERLAKLKAHLLEHPTTYIEIIEQIPVKDKTHLYQFLAQVENLQGEGVVVRNPNAPYERKRSSQILKLKTARDEECT
VIAHHKGKGQFENVMGALTCKNHRGEFKIGSGFNLNERENPPPIGSVITYKYRGITNSGKPRFATYWREKK

Sequences:

>Translated_231_residues
MSEKLDGVRGYWDGKQLLTRQGQPLSPPAYFIKDFPPFAIDGELFSERNHFEEISSITKSFKGDGWAKLKLYVFDVPDAE
GNLFERLAKLKAHLLEHPTTYIEIIEQIPVKDKTHLYQFLAQVENLQGEGVVVRNPNAPYERKRSSQILKLKTARDEECT
VIAHHKGKGQFENVMGALTCKNHRGEFKIGSGFNLNERENPPPIGSVITYKYRGITNSGKPRFATYWREKK
>Mature_230_residues
SEKLDGVRGYWDGKQLLTRQGQPLSPPAYFIKDFPPFAIDGELFSERNHFEEISSITKSFKGDGWAKLKLYVFDVPDAEG
NLFERLAKLKAHLLEHPTTYIEIIEQIPVKDKTHLYQFLAQVENLQGEGVVVRNPNAPYERKRSSQILKLKTARDEECTV
IAHHKGKGQFENVMGALTCKNHRGEFKIGSGFNLNERENPPPIGSVITYKYRGITNSGKPRFATYWREKK

Specific function: Catalyzes efficient strand joining on a single nicked DNA [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 26455; Mature: 26324

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS00333 DNA_LIGASE_A2 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLDGVRGYWDGKQLLTRQGQPLSPPAYFIKDFPPFAIDGELFSERNHFEEISSITKS
CCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHH
FKGDGWAKLKLYVFDVPDAEGNLFERLAKLKAHLLEHPTTYIEIIEQIPVKDKTHLYQFL
HCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHH
AQVENLQGEGVVVRNPNAPYERKRSSQILKLKTARDEECTVIAHHKGKGQFENVMGALTC
HHHHCCCCCEEEEECCCCCHHHHHCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHEEE
KNHRGEFKIGSGFNLNERENPPPIGSVITYKYRGITNSGKPRFATYWREKK
CCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHCCCCCC
>Mature Secondary Structure 
SEKLDGVRGYWDGKQLLTRQGQPLSPPAYFIKDFPPFAIDGELFSERNHFEEISSITKS
CCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHH
FKGDGWAKLKLYVFDVPDAEGNLFERLAKLKAHLLEHPTTYIEIIEQIPVKDKTHLYQFL
HCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHH
AQVENLQGEGVVVRNPNAPYERKRSSQILKLKTARDEECTVIAHHKGKGQFENVMGALTC
HHHHCCCCCEEEEECCCCCHHHHHCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHEEE
KNHRGEFKIGSGFNLNERENPPPIGSVITYKYRGITNSGKPRFATYWREKK
CCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550458; 7542800; 9060431 [H]