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The map label for this gene is ygbJ [C]

Identifier: 148828177

GI number: 148828177

Start: 1603214

End: 1604119

Strand: Direct

Name: ygbJ [C]

Synonym: CGSHiGG_08655

Alternate gene names: 148828177

Gene position: 1603214-1604119 (Clockwise)

Preceding gene: 148828176

Following gene: 148828178

Centisome position: 84.95

GC content: 40.29

Gene sequence:

>906_bases
ATGGAAAATCAAAATTATTCAGTCGCAGTAATTGGTCTTGGTTCTATGGGTATGGGCGCAGCGGTATCTTGCATTAATGC
AGGATTAACGACCTATGGTATTGATTTAAACCCAGTTGCACTTGAAAAACTAAAAGCGGCAGGTGCAAAAGCTGTAGCGG
CAAATGGCTATGACTTCGCTCACGAACTTGATGCTGTAGTGATTTTAGTGGTTAATGCAGCCCAAGCTAACGCAGTATTA
TTTGGCGAAAATGGTATTGCTAAAAAATTAAAAGCAGGTACAGCGGTGATGGTTTCTTCTACCATGGCGGCTCAAGATGC
TCAAATAATTTCACAAAAATTAACAGAATTAGGTTTGATTATGTTAGATGCACCTGTTTCTGGTGGTGCAGCTAAAGCAT
TAAAAGGTGAAATGACTGTTATGGCATCGGGTTCAAAACAAGCATTTGAGTTGTTACAACCTGTTTTAGATGCCACTGCG
GCAAAAGTTTATAACATTGGTGAAGAAATTGGTTTAGGTGCAACGGTGAAAATCGTCCATCAATTATTAGCTGGCGTACA
TATTGCAGCAGGTGCGGAAGCTATGGCTCTTGCCTCAAAAGCAGGTATTCCACTTGATGTTATGTATGATGTTGTGACCA
ATGCAGCTGGTAACTCTTGGATGTTTGAAAACCGGATGAAACACGTAGTAGAAGGTGACTATACGCCGCTTTCTATGGTT
GATATTTTTGTGAAAGATTTAGGCTTAGTTAATGACACTGCAAAATCACTTCATTTCCCACTTCATTTGGCAAGCACAGC
CTATTCTATGTTCACTGAGGCAAGCAATGCGGGTTATGGTAAAGAAGATGATAGTGCAGTGATTAAAATTTTTAGTGGCG
TTAGCTTACCGAAAAAAGGAGCGTAG

Upstream 100 bases:

>100_bases
ATAACATTTTTTATCATTCTGTGATCTAGATCTCAGATTTAATTTATTGTATCGGCTAATATACAGATAAATTTAGTTAA
GATCTATTGGGAGATTAAAA

Downstream 100 bases:

>100_bases
CGATGTTAGGTGTTATTGCAGATGATTTTACAGGCGCAAGCGATATCGCCAGTTTTTGGTTGAAAATGGCTTAAGTACGG
TGCAAATGAACGGTGTTCCA

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MENQNYSVAVIGLGSMGMGAAVSCINAGLTTYGIDLNPVALEKLKAAGAKAVAANGYDFAHELDAVVILVVNAAQANAVL
FGENGIAKKLKAGTAVMVSSTMAAQDAQIISQKLTELGLIMLDAPVSGGAAKALKGEMTVMASGSKQAFELLQPVLDATA
AKVYNIGEEIGLGATVKIVHQLLAGVHIAAGAEAMALASKAGIPLDVMYDVVTNAAGNSWMFENRMKHVVEGDYTPLSMV
DIFVKDLGLVNDTAKSLHFPLHLASTAYSMFTEASNAGYGKEDDSAVIKIFSGVSLPKKGA

Sequences:

>Translated_301_residues
MENQNYSVAVIGLGSMGMGAAVSCINAGLTTYGIDLNPVALEKLKAAGAKAVAANGYDFAHELDAVVILVVNAAQANAVL
FGENGIAKKLKAGTAVMVSSTMAAQDAQIISQKLTELGLIMLDAPVSGGAAKALKGEMTVMASGSKQAFELLQPVLDATA
AKVYNIGEEIGLGATVKIVHQLLAGVHIAAGAEAMALASKAGIPLDVMYDVVTNAAGNSWMFENRMKHVVEGDYTPLSMV
DIFVKDLGLVNDTAKSLHFPLHLASTAYSMFTEASNAGYGKEDDSAVIKIFSGVSLPKKGA
>Mature_301_residues
MENQNYSVAVIGLGSMGMGAAVSCINAGLTTYGIDLNPVALEKLKAAGAKAVAANGYDFAHELDAVVILVVNAAQANAVL
FGENGIAKKLKAGTAVMVSSTMAAQDAQIISQKLTELGLIMLDAPVSGGAAKALKGEMTVMASGSKQAFELLQPVLDATA
AKVYNIGEEIGLGATVKIVHQLLAGVHIAAGAEAMALASKAGIPLDVMYDVVTNAAGNSWMFENRMKHVVEGDYTPLSMV
DIFVKDLGLVNDTAKSLHFPLHLASTAYSMFTEASNAGYGKEDDSAVIKIFSGVSLPKKGA

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=28.5223367697594, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI40556376, Length=287, Percent_Identity=20.5574912891986, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1789092, Length=294, Percent_Identity=72.4489795918367, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=32.8571428571429, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1790315, Length=284, Percent_Identity=30.2816901408451, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1786719, Length=279, Percent_Identity=28.673835125448, Blast_Score=111, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17557316, Length=273, Percent_Identity=27.1062271062271, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24655230, Length=288, Percent_Identity=26.7361111111111, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922568, Length=288, Percent_Identity=26.7361111111111, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28574115, Length=279, Percent_Identity=24.7311827956989, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1010_HAEIN (P44979)

Other databases:

- EMBL:   L42023
- PIR:   B64164
- RefSeq:   NP_439171.1
- ProteinModelPortal:   P44979
- SMR:   P44979
- GeneID:   950607
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1010
- TIGR:   HI_1010
- HOGENOM:   HBG729179
- OMA:   SAGFGRE
- ProtClustDB:   CLSK2752809
- BioCyc:   HINF71421:HI_1010-MONOMER
- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR22981

Pfam domain/function: PF03446 NAD_binding_2; SSF48179 6DGDH_C_like

EC number: 1.1.1.31

Molecular weight: Translated: 31035; Mature: 31035

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: ACT_SITE 177-177 BINDING 101-101 BINDING 245-245

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENQNYSVAVIGLGSMGMGAAVSCINAGLTTYGIDLNPVALEKLKAAGAKAVAANGYDFA
CCCCCEEEEEEEECCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCEEECCCCCHH
HELDAVVILVVNAAQANAVLFGENGIAKKLKAGTAVMVSSTMAAQDAQIISQKLTELGLI
HHHCEEEEEEEECCCCCEEEECCCCHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCEE
MLDAPVSGGAAKALKGEMTVMASGSKQAFELLQPVLDATAAKVYNIGEEIGLGATVKIVH
EEECCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHH
QLLAGVHIAAGAEAMALASKAGIPLDVMYDVVTNAAGNSWMFENRMKHVVEGDYTPLSMV
HHHHCCCHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHH
DIFVKDLGLVNDTAKSLHFPLHLASTAYSMFTEASNAGYGKEDDSAVIKIFSGVSLPKKG
HHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCCCCCCCC
A
C
>Mature Secondary Structure
MENQNYSVAVIGLGSMGMGAAVSCINAGLTTYGIDLNPVALEKLKAAGAKAVAANGYDFA
CCCCCEEEEEEEECCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCEEECCCCCHH
HELDAVVILVVNAAQANAVLFGENGIAKKLKAGTAVMVSSTMAAQDAQIISQKLTELGLI
HHHCEEEEEEEECCCCCEEEECCCCHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCEE
MLDAPVSGGAAKALKGEMTVMASGSKQAFELLQPVLDATAAKVYNIGEEIGLGATVKIVH
EEECCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHH
QLLAGVHIAAGAEAMALASKAGIPLDVMYDVVTNAAGNSWMFENRMKHVVEGDYTPLSMV
HHHHCCCHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHH
DIFVKDLGLVNDTAKSLHFPLHLASTAYSMFTEASNAGYGKEDDSAVIKIFSGVSLPKKG
HHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800