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The map label for this gene is trmE [H]

Identifier: 148828171

GI number: 148828171

Start: 1595136

End: 1596494

Strand: Direct

Name: trmE [H]

Synonym: CGSHiGG_08615

Alternate gene names: 148828171

Gene position: 1595136-1596494 (Clockwise)

Preceding gene: 148828170

Following gene: 148828172

Centisome position: 84.52

GC content: 39.96

Gene sequence:

>1359_bases
ATGAAAGAAACAATCGTTGCTCAAGCTACTGCACCAGGTCGTGGTGGTATTGGGATTTTAAGAGTCTCTGGACCTTTAGC
AACAGAAGTTGCTCAAGCTATATTAGGTAAATGCCCTAAGCCAAGAATGGCAGATTATCTTCCTTTTAAAGATGCTGATG
GCACTATTTTAGATCAAGGGATTGCGCTTTATTTCAAATCGCCAAATTCATTTACTGGTGAAGATGTGTTGGAATTGCAA
GGTCACGGTGGGCAGGTTGTATTAGATTTATTACTCAAACGTATTTTACAAATTGACGGAATCCGTTTGGCGAGACCAGG
CGAGTTTTCTGAACAAGCTTTTTTGAATGATAAATTTGATCTTGCCCAAGCAGAGGCCATTGCAGATTTAATTGATGCAA
CATCAGAACAAGCGGCACGCTCTGCATTGAAATCCTTACAAGGCGAGTTTTCTAAAAAAGTAAATGAATTGGTTGATTCT
GTGATTTATTTACGTACTTATGTGGAAGCAAGCATTGATTTTCCTGATGAAGAAATTGATTTCTTGGCAGATGGAAAAAT
TGAAGCAAACTTACGCAGTATTATAAATCAGTTGGAAGATGTTCGCGCTGAAGCTAAACAAGGTTCTATTTTACGTGAGG
GGATGAAAGTCGTGATTGCTGGTCGCCCGAATGCAGGCAAATCGAGTTTGCTTAATGCACTTGCTGGTCGCGAAGCGGCT
ATCGTCACGGATATTGCAGGTACAACCCGTGATGTATTGCGTGAACATATTCATATTGACGGTATGCCATTGCATATTAT
TGATACCGCAGGTCTTCGAGATGCAACCGATGAAGTGGAGCGTATTGGCATTTCTCGTGCTTGGACAGAGATTGAGCAAG
CCGATCGAATTATTTTGATGTTAGATAGTAGCGATCCTGAAAGTGTAGATTTAAGCAAAGTGCGGTCAGAATTTTTAGCA
AAATTACCCTCAACCTTGCCAGTTACTATTGTTAGAAACAAAATTGATTTAAATGGCGAACAAGCAAGTGAAAGCGAACA
GAGCGGTTATCAAATGATTAGTCTTTCCGCTCAAACGCATGATGGCGTAAAACTTTTACGTGAACATTTGAAACAAGCAA
TGGGTTTCCAAACAGGCATGGAAGGAGGCTTTTTAGCTCGCCGCCGTCATTTAGATGCGTTGGATAAAGCCGCAGAGCAT
TTACAAATCGGTTTGGTTCAACTTACAGAATTTCACGCAGGAGAACTTTTAGCGGAAGAATTACGCTTAGTTCAAAGCTA
TTTAAGTGAAATTACGGGTCAATTCACATCTGATGATTTACTTGGTAATATTTTTAGTTCTTTTTGCATTGGAAAATAG

Upstream 100 bases:

>100_bases
CCCCTTTGGGGAACATTAAGCTCTACCCCAAAGGGGGAGCACTGGAATGAACCTAGGGTATACTTACCCTAGGTCTACCC
CAGACAATAATAGGTTTTTT

Downstream 100 bases:

>100_bases
GAAGCGTAAAGTGCGGTCATTTTTTGGCAAAATTTAAATCAAAAGCGGCAATATTTGGTGGTGTTTTGTTGATAGGAATT
GCTATTAGCTGGCTTTGTGA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKETIVAQATAPGRGGIGILRVSGPLATEVAQAILGKCPKPRMADYLPFKDADGTILDQGIALYFKSPNSFTGEDVLELQ
GHGGQVVLDLLLKRILQIDGIRLARPGEFSEQAFLNDKFDLAQAEAIADLIDATSEQAARSALKSLQGEFSKKVNELVDS
VIYLRTYVEASIDFPDEEIDFLADGKIEANLRSIINQLEDVRAEAKQGSILREGMKVVIAGRPNAGKSSLLNALAGREAA
IVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDATDEVERIGISRAWTEIEQADRIILMLDSSDPESVDLSKVRSEFLA
KLPSTLPVTIVRNKIDLNGEQASESEQSGYQMISLSAQTHDGVKLLREHLKQAMGFQTGMEGGFLARRRHLDALDKAAEH
LQIGLVQLTEFHAGELLAEELRLVQSYLSEITGQFTSDDLLGNIFSSFCIGK

Sequences:

>Translated_452_residues
MKETIVAQATAPGRGGIGILRVSGPLATEVAQAILGKCPKPRMADYLPFKDADGTILDQGIALYFKSPNSFTGEDVLELQ
GHGGQVVLDLLLKRILQIDGIRLARPGEFSEQAFLNDKFDLAQAEAIADLIDATSEQAARSALKSLQGEFSKKVNELVDS
VIYLRTYVEASIDFPDEEIDFLADGKIEANLRSIINQLEDVRAEAKQGSILREGMKVVIAGRPNAGKSSLLNALAGREAA
IVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDATDEVERIGISRAWTEIEQADRIILMLDSSDPESVDLSKVRSEFLA
KLPSTLPVTIVRNKIDLNGEQASESEQSGYQMISLSAQTHDGVKLLREHLKQAMGFQTGMEGGFLARRRHLDALDKAAEH
LQIGLVQLTEFHAGELLAEELRLVQSYLSEITGQFTSDDLLGNIFSSFCIGK
>Mature_452_residues
MKETIVAQATAPGRGGIGILRVSGPLATEVAQAILGKCPKPRMADYLPFKDADGTILDQGIALYFKSPNSFTGEDVLELQ
GHGGQVVLDLLLKRILQIDGIRLARPGEFSEQAFLNDKFDLAQAEAIADLIDATSEQAARSALKSLQGEFSKKVNELVDS
VIYLRTYVEASIDFPDEEIDFLADGKIEANLRSIINQLEDVRAEAKQGSILREGMKVVIAGRPNAGKSSLLNALAGREAA
IVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDATDEVERIGISRAWTEIEQADRIILMLDSSDPESVDLSKVRSEFLA
KLPSTLPVTIVRNKIDLNGEQASESEQSGYQMISLSAQTHDGVKLLREHLKQAMGFQTGMEGGFLARRRHLDALDKAAEH
LQIGLVQLTEFHAGELLAEELRLVQSYLSEITGQFTSDDLLGNIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=467, Percent_Identity=34.9036402569593, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=32.8657314629259, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI193082993, Length=461, Percent_Identity=32.1041214750542, Blast_Score=206, Evalue=5e-53,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=74.9445676274945, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=94, Percent_Identity=39.3617021276596, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=451, Percent_Identity=28.8248337028825, Blast_Score=165, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6323665, Length=498, Percent_Identity=32.9317269076305, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI45550813, Length=472, Percent_Identity=30.9322033898305, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49300; Mature: 49300

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETIVAQATAPGRGGIGILRVSGPLATEVAQAILGKCPKPRMADYLPFKDADGTILDQG
CCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCC
IALYFKSPNSFTGEDVLELQGHGGQVVLDLLLKRILQIDGIRLARPGEFSEQAFLNDKFD
EEEEEECCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHCCEEECCCCCCCHHHHCCCCHH
LAQAEAIADLIDATSEQAARSALKSLQGEFSKKVNELVDSVIYLRTYVEASIDFPDEEID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHC
FLADGKIEANLRSIINQLEDVRAEAKQGSILREGMKVVIAGRPNAGKSSLLNALAGREAA
EEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCE
IVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDATDEVERIGISRAWTEIEQADRIILM
EEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEE
LDSSDPESVDLSKVRSEFLAKLPSTLPVTIVRNKIDLNGEQASESEQSGYQMISLSAQTH
EECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECEECCCCCCCCCHHHCCCEEEEEECCCC
DGVKLLREHLKQAMGFQTGMEGGFLARRRHLDALDKAAEHLQIGLVQLTEFHAGELLAEE
HHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLVQSYLSEITGQFTSDDLLGNIFSSFCIGK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKETIVAQATAPGRGGIGILRVSGPLATEVAQAILGKCPKPRMADYLPFKDADGTILDQG
CCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCC
IALYFKSPNSFTGEDVLELQGHGGQVVLDLLLKRILQIDGIRLARPGEFSEQAFLNDKFD
EEEEEECCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHCCEEECCCCCCCHHHHCCCCHH
LAQAEAIADLIDATSEQAARSALKSLQGEFSKKVNELVDSVIYLRTYVEASIDFPDEEID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHC
FLADGKIEANLRSIINQLEDVRAEAKQGSILREGMKVVIAGRPNAGKSSLLNALAGREAA
EEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCE
IVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDATDEVERIGISRAWTEIEQADRIILM
EEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEE
LDSSDPESVDLSKVRSEFLAKLPSTLPVTIVRNKIDLNGEQASESEQSGYQMISLSAQTH
EECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECEECCCCCCCCCHHHCCCEEEEEECCCC
DGVKLLREHLKQAMGFQTGMEGGFLARRRHLDALDKAAEHLQIGLVQLTEFHAGELLAEE
HHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLVQSYLSEITGQFTSDDLLGNIFSSFCIGK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA