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The map label for this gene is leuC2 [H]

Identifier: 148828161

GI number: 148828161

Start: 1574323

End: 1575690

Strand: Direct

Name: leuC2 [H]

Synonym: CGSHiGG_08525

Alternate gene names: 148828161

Gene position: 1574323-1575690 (Clockwise)

Preceding gene: 148828155

Following gene: 148828162

Centisome position: 83.42

GC content: 43.64

Gene sequence:

>1368_bases
GTGTATGAAGCAGAGGGCGAAACGCCGATTTTGTATATCAACCGTCATTTGATTCACGAAGTAACCAGCCCGCAGGCCTT
TGATGGGTTACGCGTCGCAAATCGCCAAGTACGTCAAGTAAACAAAACTTTTGGCACAATGGATCACAGCATTTCTACTC
AAGTACGAGATGTCAATAAACTTGAAGGTCAGGCTAAAATTCAAGTGTTAGAACTGGATAAAAATACCAAAGCAACAGGC
ATTAAATTATTTGATATAACAACCAAAGAGCAAGGCATTGTACACGTTATGGGGCCTGAACAAGGTTTAACCTTACCGGG
TATGACAATTGTTTGTGGCGATTCACACACCGCAACGCACGGTGCATTTGGTGCCTTGGCATTTGGTATTGGTACTTCTG
AAGTAGAACACGTATTAGCCACCCAAACTTTAAAACAGGCTCGCGCAAAAAGTATGAAAATTGAAGTGCGTGGTAAAGTT
GCATCAGGTATTACGGCAAAAGATATTATTCTTGCCATTATCGGTAAAACCACTATGGCAGGCGGAACAGGACACGTTGT
AGAATTCTGCGGCGAAGCGATCCGAGACCTTTCTATGGAGGGACGTATGACCGTCTGTAATATGGCAATTGAAATGGGTG
CAAAAGCAGGCTTGATTGCGCCAGACGAAACCACATTTGCATATTTGAAAGATCGTCCACATGCACCTAAAGGCAAGGAT
TGGGATGATGCCGTAGCTTATTGGAAAACCTTAAAATCTGACGATGATGCACAATTTGACACTGTGGTAACGCTAGAAGC
AAAAGACATCGCACCACAAGTCACTTGGGGGACAAACCCAGGGCAGGTAATTTCTGTGAATGAAACTATACCAAATCCGC
AAGAAATGGCAGATCCTGTACAACGTGCTTCGGCAGAAAAAGCCTTACATTATATTGGCTTGGAAGCTGGCACTAATTTA
AAAGATATTAAAGTTGATCAAGTATTTATTGGATCTTGCACCAACTCGCGTATTGAAGATTTACGCGCAGCAGCGGCTGT
AATGAAAGGTCGTAAAAAAGCGGATAATGTAAAACGTATTTTAGTAGTACCAGGTTCTGGCTTAGTGAAAGAACAAGCGG
AAAAAGAGGGCTTGGATAAAATCTTTATTGCCGCAGGTGCAGAATGGCGTAATCCGGGCTGCTCAATGTGCTTAGGGATG
AACGACGACCGTTTAGGCGAATGGGAACGTTGTGCTTCCACGTCAAACCGTAACTTTGAAGGTCGCCAAGGCCGTAATGG
ACGCACCCATTTAGTAAGTCCTGCAATGGCAGCGGCAGCAGGAGTGTTCGGTAAATTTGTTGATATTCGTGACGTAACAT
TAAACTAA

Upstream 100 bases:

>100_bases
GTTGAAAAACACGTCGAATTTTAACCGCACTTCCCTTAACGAATAGAGAAAAATATTATGGCAAAAAACACTTTACGAAA
AATTATTTGATTCACACATT

Downstream 100 bases:

>100_bases
GGAGCAGAAAATGGCAGGATTTAAACAACTTTCAGGCTTAGTCGTACCATTGGATGCGGCAAATGTAGATACCGATGCAA
TTATTCCAAAACAATTTTTA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MYEAEGETPILYINRHLIHEVTSPQAFDGLRVANRQVRQVNKTFGTMDHSISTQVRDVNKLEGQAKIQVLELDKNTKATG
IKLFDITTKEQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQARAKSMKIEVRGKV
ASGITAKDIILAIIGKTTMAGGTGHVVEFCGEAIRDLSMEGRMTVCNMAIEMGAKAGLIAPDETTFAYLKDRPHAPKGKD
WDDAVAYWKTLKSDDDAQFDTVVTLEAKDIAPQVTWGTNPGQVISVNETIPNPQEMADPVQRASAEKALHYIGLEAGTNL
KDIKVDQVFIGSCTNSRIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKEGLDKIFIAAGAEWRNPGCSMCLGM
NDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAGVFGKFVDIRDVTLN

Sequences:

>Translated_455_residues
MYEAEGETPILYINRHLIHEVTSPQAFDGLRVANRQVRQVNKTFGTMDHSISTQVRDVNKLEGQAKIQVLELDKNTKATG
IKLFDITTKEQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQARAKSMKIEVRGKV
ASGITAKDIILAIIGKTTMAGGTGHVVEFCGEAIRDLSMEGRMTVCNMAIEMGAKAGLIAPDETTFAYLKDRPHAPKGKD
WDDAVAYWKTLKSDDDAQFDTVVTLEAKDIAPQVTWGTNPGQVISVNETIPNPQEMADPVQRASAEKALHYIGLEAGTNL
KDIKVDQVFIGSCTNSRIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKEGLDKIFIAAGAEWRNPGCSMCLGM
NDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAGVFGKFVDIRDVTLN
>Mature_455_residues
MYEAEGETPILYINRHLIHEVTSPQAFDGLRVANRQVRQVNKTFGTMDHSISTQVRDVNKLEGQAKIQVLELDKNTKATG
IKLFDITTKEQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQARAKSMKIEVRGKV
ASGITAKDIILAIIGKTTMAGGTGHVVEFCGEAIRDLSMEGRMTVCNMAIEMGAKAGLIAPDETTFAYLKDRPHAPKGKD
WDDAVAYWKTLKSDDDAQFDTVVTLEAKDIAPQVTWGTNPGQVISVNETIPNPQEMADPVQRASAEKALHYIGLEAGTNL
KDIKVDQVFIGSCTNSRIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKEGLDKIFIAAGAEWRNPGCSMCLGM
NDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAGVFGKFVDIRDVTLN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=361, Percent_Identity=26.8698060941828, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI8659555, Length=447, Percent_Identity=26.3982102908277, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI41352693, Length=378, Percent_Identity=25.9259259259259, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI1786259, Length=452, Percent_Identity=76.1061946902655, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI87081781, Length=325, Percent_Identity=26.4615384615385, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787531, Length=362, Percent_Identity=25.414364640884, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI2367097, Length=354, Percent_Identity=25.7062146892655, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=362, Percent_Identity=29.5580110497238, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=363, Percent_Identity=29.4765840220386, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=304, Percent_Identity=29.6052631578947, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=462, Percent_Identity=25.974025974026, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=457, Percent_Identity=60.6126914660831, Blast_Score=565, Evalue=1e-162,
Organism=Saccharomyces cerevisiae, GI6323335, Length=406, Percent_Identity=29.3103448275862, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=369, Percent_Identity=29.2682926829268, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6320440, Length=429, Percent_Identity=25.8741258741259, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI281365315, Length=362, Percent_Identity=25.9668508287293, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17864292, Length=362, Percent_Identity=25.9668508287293, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161076999, Length=363, Percent_Identity=25.8953168044077, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28571643, Length=365, Percent_Identity=26.3013698630137, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24645686, Length=460, Percent_Identity=26.5217391304348, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137564, Length=459, Percent_Identity=27.2331154684096, Blast_Score=95, Evalue=9e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 49243; Mature: 49243

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEAEGETPILYINRHLIHEVTSPQAFDGLRVANRQVRQVNKTFGTMDHSISTQVRDVNK
CCCCCCCCCEEEEEHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LEGQAKIQVLELDKNTKATGIKLFDITTKEQGIVHVMGPEQGLTLPGMTIVCGDSHTATH
CCCCEEEEEEEECCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCCCCC
GAFGALAFGIGTSEVEHVLATQTLKQARAKSMKIEVRGKVASGITAKDIILAIIGKTTMA
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHEECCCCCC
GGTGHVVEFCGEAIRDLSMEGRMTVCNMAIEMGAKAGLIAPDETTFAYLKDRPHAPKGKD
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCC
WDDAVAYWKTLKSDDDAQFDTVVTLEAKDIAPQVTWGTNPGQVISVNETIPNPQEMADPV
HHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCEEEECCCCCEEEEECCCCCCHHHHHHHH
QRASAEKALHYIGLEAGTNLKDIKVDQVFIGSCTNSRIEDLRAAAAVMKGRKKADNVKRI
HHHHHHHHHHEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEE
LVVPGSGLVKEQAEKEGLDKIFIAAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFE
EEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCC
GRQGRNGRTHLVSPAMAAAAGVFGKFVDIRDVTLN
CCCCCCCCEEECCHHHHHHHHHHHHHEEEEEEECC
>Mature Secondary Structure
MYEAEGETPILYINRHLIHEVTSPQAFDGLRVANRQVRQVNKTFGTMDHSISTQVRDVNK
CCCCCCCCCEEEEEHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LEGQAKIQVLELDKNTKATGIKLFDITTKEQGIVHVMGPEQGLTLPGMTIVCGDSHTATH
CCCCEEEEEEEECCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCCCCC
GAFGALAFGIGTSEVEHVLATQTLKQARAKSMKIEVRGKVASGITAKDIILAIIGKTTMA
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHEECCCCCC
GGTGHVVEFCGEAIRDLSMEGRMTVCNMAIEMGAKAGLIAPDETTFAYLKDRPHAPKGKD
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCC
WDDAVAYWKTLKSDDDAQFDTVVTLEAKDIAPQVTWGTNPGQVISVNETIPNPQEMADPV
HHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCEEEECCCCCEEEEECCCCCCHHHHHHHH
QRASAEKALHYIGLEAGTNLKDIKVDQVFIGSCTNSRIEDLRAAAAVMKGRKKADNVKRI
HHHHHHHHHHEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEE
LVVPGSGLVKEQAEKEGLDKIFIAAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFE
EEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCC
GRQGRNGRTHLVSPAMAAAAGVFGKFVDIRDVTLN
CCCCCCCCEEECCHHHHHHHHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA