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The map label for this gene is rffG [H]

Identifier: 148828056

GI number: 148828056

Start: 1456742

End: 1457746

Strand: Direct

Name: rffG [H]

Synonym: CGSHiGG_07900

Alternate gene names: 148828056

Gene position: 1456742-1457746 (Clockwise)

Preceding gene: 148828055

Following gene: 148828057

Centisome position: 77.19

GC content: 37.61

Gene sequence:

>1005_bases
ATGAATATTTTAATCACCGGCGGTTCCGGCTTCATCGGTTCCGCTCTTATTCGATATATCATTTATCAGACTCAAGATTC
CGTTATCAATATTGATAAACTGACCTATGCGGCAAATCAATCAGCTTTGAAAGAGGTTGAAAATAATCCTCGCTATGCTT
TTGAGCAAATTGATATCTGTGATCTTAAGGCGATAGAAAGCGTTTTCGAGAAATATCAGCCTGATGCAGTGATGCATTTG
GCGGCAGAAAGTCATGTCGATCGTTCTATTTCTGGAGCAGCTGATTTTATTCAAACCAATATTGTTGGTACTTATACGTT
GTTAGAGGTTGCTAAGAATTATTGGCATACCTTAGACGAAGCTAAAAAAACGACCTTTCGATTCCACCATATTTCTACTG
ATGAAGTTTATGGTGATTTATCTCTTTCAGAACCTGCTTTTACCGAATATTCTCCTTATCATCCAAGCAGCCCTTATTCA
GCATCAAAAGCAGCGAGTGATCATTTAGTTTATGCTTGGCATCGCACTTATGGCTTGCCGGTGATTATTACAAACAGTTC
CAATAACTATGGAGCTTACCAACATCCGGAAAAACTCATCCCTTTAATGATTTCAAATGCACTGATGGGGAAGCCTTTGC
CAATTTATGGTGATGGGCAGCAGATTCGCGATTGGTTATTTGTGGAAGAGCATGTTCAAGCCTTATATTTAGTTTTAACA
AAAGGTCGAGTCGGGGAAAACTATAATATCGGTGGAAATTGTGAAAAAACAAACCTTGAAGTGGTTAAAACAATTTGTCA
ATTACTCGAAGAACTTGCACCAAATAAGCCTAATAATATTAAGTATTACGATGATTTAATTATTTTTGTAAAAGACCGAC
CTGGTCACGATGTGCGATATTCATTGGATTGTTCCAAAATTCATGCAGAATTAGGTTGGCAACCGAAAATAACCTTTGAA
CGAGGACTTAGCCAGACGGTACAATGGTATTTGTTTAACAGTTAA

Upstream 100 bases:

>100_bases
TAGTTTTAAAACGAGATGGAGCTTATTAATGTTTTATTTAATTATTTCTTAAGCGAGATTTCTTCTATTTGTATTACTTT
AAATTTATTAACTCAACAAG

Downstream 100 bases:

>100_bases
TCTTCTTAAATAGGGTAAAAGATAAGAATGTTTATCTATCTTGTGATTTTTTCTCTTTTTATTAGACAATAAAATATAAG
ATAGTTGAAGAAGTAAAAAA

Product: dTDP-glucose 46-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MNILITGGSGFIGSALIRYIIYQTQDSVINIDKLTYAANQSALKEVENNPRYAFEQIDICDLKAIESVFEKYQPDAVMHL
AAESHVDRSISGAADFIQTNIVGTYTLLEVAKNYWHTLDEAKKTTFRFHHISTDEVYGDLSLSEPAFTEYSPYHPSSPYS
ASKAASDHLVYAWHRTYGLPVIITNSSNNYGAYQHPEKLIPLMISNALMGKPLPIYGDGQQIRDWLFVEEHVQALYLVLT
KGRVGENYNIGGNCEKTNLEVVKTICQLLEELAPNKPNNIKYYDDLIIFVKDRPGHDVRYSLDCSKIHAELGWQPKITFE
RGLSQTVQWYLFNS

Sequences:

>Translated_334_residues
MNILITGGSGFIGSALIRYIIYQTQDSVINIDKLTYAANQSALKEVENNPRYAFEQIDICDLKAIESVFEKYQPDAVMHL
AAESHVDRSISGAADFIQTNIVGTYTLLEVAKNYWHTLDEAKKTTFRFHHISTDEVYGDLSLSEPAFTEYSPYHPSSPYS
ASKAASDHLVYAWHRTYGLPVIITNSSNNYGAYQHPEKLIPLMISNALMGKPLPIYGDGQQIRDWLFVEEHVQALYLVLT
KGRVGENYNIGGNCEKTNLEVVKTICQLLEELAPNKPNNIKYYDDLIIFVKDRPGHDVRYSLDCSKIHAELGWQPKITFE
RGLSQTVQWYLFNS
>Mature_334_residues
MNILITGGSGFIGSALIRYIIYQTQDSVINIDKLTYAANQSALKEVENNPRYAFEQIDICDLKAIESVFEKYQPDAVMHL
AAESHVDRSISGAADFIQTNIVGTYTLLEVAKNYWHTLDEAKKTTFRFHHISTDEVYGDLSLSEPAFTEYSPYHPSSPYS
ASKAASDHLVYAWHRTYGLPVIITNSSNNYGAYQHPEKLIPLMISNALMGKPLPIYGDGQQIRDWLFVEEHVQALYLVLT
KGRVGENYNIGGNCEKTNLEVVKTICQLLEELAPNKPNNIKYYDDLIIFVKDRPGHDVRYSLDCSKIHAELGWQPKITFE
RGLSQTVQWYLFNS

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=39.1566265060241, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI42516563, Length=333, Percent_Identity=24.6246246246246, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI4504031, Length=347, Percent_Identity=24.207492795389, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI56237023, Length=368, Percent_Identity=23.9130434782609, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI56118217, Length=368, Percent_Identity=23.9130434782609, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI189083684, Length=368, Percent_Identity=23.9130434782609, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI48994969, Length=331, Percent_Identity=67.97583081571, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=62.5730994152047, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1788366, Length=354, Percent_Identity=25.1412429378531, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788365, Length=353, Percent_Identity=21.5297450424929, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=332, Percent_Identity=37.6506024096386, Blast_Score=207, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=35.6495468277946, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17539532, Length=333, Percent_Identity=23.1231231231231, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=365, Percent_Identity=25.7534246575342, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=367, Percent_Identity=25.6130790190736, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=341, Percent_Identity=24.3401759530792, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=341, Percent_Identity=24.3401759530792, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=341, Percent_Identity=24.3401759530792, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=338, Percent_Identity=23.9644970414201, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=341, Percent_Identity=24.3401759530792, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=341, Percent_Identity=24.3401759530792, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=360, Percent_Identity=28.0555555555556, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356223, Length=333, Percent_Identity=25.8258258258258, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19923002, Length=361, Percent_Identity=25.4847645429363, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 37887; Mature: 37887

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILITGGSGFIGSALIRYIIYQTQDSVINIDKLTYAANQSALKEVENNPRYAFEQIDIC
CEEEEECCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHCCHHHHHHHHCCCCEEECCCCCH
DLKAIESVFEKYQPDAVMHLAAESHVDRSISGAADFIQTNIVGTYTLLEVAKNYWHTLDE
HHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AKKTTFRFHHISTDEVYGDLSLSEPAFTEYSPYHPSSPYSASKAASDHLVYAWHRTYGLP
HHHHEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEEEECCCCCE
VIITNSSNNYGAYQHPEKLIPLMISNALMGKPLPIYGDGQQIRDWLFVEEHVQALYLVLT
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEE
KGRVGENYNIGGNCEKTNLEVVKTICQLLEELAPNKPNNIKYYDDLIIFVKDRPGHDVRY
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCEEE
SLDCSKIHAELGWQPKITFERGLSQTVQWYLFNS
ECCHHHHHHHCCCCCCCHHHHCCCCEEEEEEECC
>Mature Secondary Structure
MNILITGGSGFIGSALIRYIIYQTQDSVINIDKLTYAANQSALKEVENNPRYAFEQIDIC
CEEEEECCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHCCHHHHHHHHCCCCEEECCCCCH
DLKAIESVFEKYQPDAVMHLAAESHVDRSISGAADFIQTNIVGTYTLLEVAKNYWHTLDE
HHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AKKTTFRFHHISTDEVYGDLSLSEPAFTEYSPYHPSSPYSASKAASDHLVYAWHRTYGLP
HHHHEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEEEECCCCCE
VIITNSSNNYGAYQHPEKLIPLMISNALMGKPLPIYGDGQQIRDWLFVEEHVQALYLVLT
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEE
KGRVGENYNIGGNCEKTNLEVVKTICQLLEELAPNKPNNIKYYDDLIIFVKDRPGHDVRY
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCEEE
SLDCSKIHAELGWQPKITFERGLSQTVQWYLFNS
ECCHHHHHHHCCCCCCCHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]