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The map label for this gene is gph [C]

Identifier: 148827757

GI number: 148827757

Start: 1109508

End: 1110182

Strand: Reverse

Name: gph [C]

Synonym: CGSHiGG_06100

Alternate gene names: 148827757

Gene position: 1110182-1109508 (Counterclockwise)

Preceding gene: 148827758

Following gene: 148827755

Centisome position: 58.83

GC content: 38.37

Gene sequence:

>675_bases
ATGAATACACAATTTAAACTTATTGGCTTTGATTTAGACGGCACCTTGGTAAATAGCTTGCCTGATTTAGCGTTATCCGT
AAATTCTGCTTTGGCTGAATTTGATTTACCGAAAGCGCCAGAAGAATTAGTTTTAACTTGGATCGGTAATGGCGCGCCTG
TATTAATTGCTCGAGCGTTAGATTGGGCGAAAAAACAAACAGGAAAAGTTTTAACTGAAGAAGAGATTAAGCAAGTAACA
GAACGTTTTAATTTTTATTATGGCGAGAATTTATGTAATGTCAGCCGCTTGTATCCAAATGTAAAAGAAACTTTAGAAAC
CTTGAAAGAAAAAGGCTATGTCCTAGCAGTTGTTACGAATAAACCGACAAAGCACGTTCAACCTGTATTAGCTGCATTTG
GCATTGATCATTTATTTAGTGAAATGTTGGGCGGTCAATCCTTACCTGCCATTAAGCCACATCCAGCTCCACTTTATTAT
TTATGCGGAAAATTTGGTTTTGAACCACGCCAAGTGCTTTTCGTGGGCGATTCTAAAAATGATATTATCGCGGCTCACGC
TGCGGGCTGTGCAGTTGTTGGTTTAACTTACGGCTACAATTACAATATCCCTATCCGTGAATCCAATCCAGATTGGGTGT
TTGATGATTTTGCCCAGCTATTAAGTATTCTTTAA

Upstream 100 bases:

>100_bases
TTATAAACAAGTTATTGACCAAATGCGTACACAGTTAGCAAGTGTTAGCGCATAACTGTTATTTTAATAAAATACGATCG
TAATAGAACAGAGAAAAACA

Downstream 100 bases:

>100_bases
GTTTTCCATCTAATAAAAAGTGCGGTTAATTTTTACCGCACTTTTGTTATCTACTTAATTGATTTTAAAGAATGTTTGAA
AATTCTTCTAACATTTCTTT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MNTQFKLIGFDLDGTLVNSLPDLALSVNSALAEFDLPKAPEELVLTWIGNGAPVLIARALDWAKKQTGKVLTEEEIKQVT
ERFNFYYGENLCNVSRLYPNVKETLETLKEKGYVLAVVTNKPTKHVQPVLAAFGIDHLFSEMLGGQSLPAIKPHPAPLYY
LCGKFGFEPRQVLFVGDSKNDIIAAHAAGCAVVGLTYGYNYNIPIRESNPDWVFDDFAQLLSIL

Sequences:

>Translated_224_residues
MNTQFKLIGFDLDGTLVNSLPDLALSVNSALAEFDLPKAPEELVLTWIGNGAPVLIARALDWAKKQTGKVLTEEEIKQVT
ERFNFYYGENLCNVSRLYPNVKETLETLKEKGYVLAVVTNKPTKHVQPVLAAFGIDHLFSEMLGGQSLPAIKPHPAPLYY
LCGKFGFEPRQVLFVGDSKNDIIAAHAAGCAVVGLTYGYNYNIPIRESNPDWVFDDFAQLLSIL
>Mature_224_residues
MNTQFKLIGFDLDGTLVNSLPDLALSVNSALAEFDLPKAPEELVLTWIGNGAPVLIARALDWAKKQTGKVLTEEEIKQVT
ERFNFYYGENLCNVSRLYPNVKETLETLKEKGYVLAVVTNKPTKHVQPVLAAFGIDHLFSEMLGGQSLPAIKPHPAPLYY
LCGKFGFEPRQVLFVGDSKNDIIAAHAAGCAVVGLTYGYNYNIPIRESNPDWVFDDFAQLLSIL

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=230, Percent_Identity=47.3913043478261, Blast_Score=197, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR000150
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24791; Mature: 24791

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTQFKLIGFDLDGTLVNSLPDLALSVNSALAEFDLPKAPEELVLTWIGNGAPVLIARAL
CCCEEEEEEEECCCHHHHHCHHHHHHHHHHHHHCCCCCCCHHEEEEEECCCCCEEHHHHH
DWAKKQTGKVLTEEEIKQVTERFNFYYGENLCNVSRLYPNVKETLETLKEKGYVLAVVTN
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCEEEEEEEC
KPTKHVQPVLAAFGIDHLFSEMLGGQSLPAIKPHPAPLYYLCGKFGFEPRQVLFVGDSKN
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEHHCCCCCCCEEEEEECCCC
DIIAAHAAGCAVVGLTYGYNYNIPIRESNPDWVFDDFAQLLSIL
CEEEEECCCEEEEEEEECEECCCEEECCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MNTQFKLIGFDLDGTLVNSLPDLALSVNSALAEFDLPKAPEELVLTWIGNGAPVLIARAL
CCCEEEEEEEECCCHHHHHCHHHHHHHHHHHHHCCCCCCCHHEEEEEECCCCCEEHHHHH
DWAKKQTGKVLTEEEIKQVTERFNFYYGENLCNVSRLYPNVKETLETLKEKGYVLAVVTN
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCEEEEEEEC
KPTKHVQPVLAAFGIDHLFSEMLGGQSLPAIKPHPAPLYYLCGKFGFEPRQVLFVGDSKN
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEHHCCCCCCCEEEEEECCCC
DIIAAHAAGCAVVGLTYGYNYNIPIRESNPDWVFDDFAQLLSIL
CEEEEECCCEEEEEEEECEECCCEEECCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA