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The map label for this gene is aspA [H]

Identifier: 148827726

GI number: 148827726

Start: 1082152

End: 1083570

Strand: Reverse

Name: aspA [H]

Synonym: CGSHiGG_05925

Alternate gene names: 148827726

Gene position: 1083570-1082152 (Counterclockwise)

Preceding gene: 148827727

Following gene: 148827721

Centisome position: 57.42

GC content: 37.77

Gene sequence:

>1419_bases
ATGACTCAATTTAGAAAAGAAGTAGATTTACTTGGCGAACGTGATGTGCCTGCAGAAGCATATTGGGGGATTCATACATT
AAGAGCGGTAGAAAATTTCAATATTTCTAACGTAACCATTTCTGACGTGCCTGAGTTTGTACGTGGTATGGTAATGGTAA
AAAAAGCAACGGCTTTAGCAAATGGTGAATTAGGTGCAATTCCAAGTGATATTGCAAAAGCGATTGTAGCAGCTTGTGAT
GAAATTCTTACCACTGGAAAATGCTTAGATCAATTCCCATCAGATGTATATCAAGGTGGTGCAGGTACCTCAGTGAATAT
GAATACCAATGAAGTTGTGGCGAATTTGGCGTTAGAGTTATTAGGACATAAGAAAGGGGAATACCAATATTTAGATCCAA
TGGATCATGTTAACGCTAGCCAATCAACCAACGATGCGTATCCTACCGGTTTCCGTATTGCAGTGTATAACAGCATCTTA
AAATTGATCGATAAAATTCAATATTTACACGACGGTTTTGATAATAAAGCAAAAGAGTTTGCAAATATCTTAAAAATGGG
TCGTACCCAATTGCAAGATGCGGTGCCAATGACTGTTGGTCAAGAATTCAAAGCTTTCGCCGTATTACTTGAAGAAGAAG
TGCGTAACTTAAAACGTACAGCAGGTTTATTACTTGAAGTAAACCTTGGTGCGACTGCAATCGGTACTGGTTTAAATACG
CCACAAGGCTATACAGAATTAGTTGTAAAACATCTTGCTGAAGTGACTGGATTAGCTTGTGTACCAGCAGAAAACTTAAT
TGAAGCAACATCTGACTGTGGCGCTTATGTCATGGTTCACGGTGCATTAAAACGTACTGCAGTGAAACTTTCTAAAGTAT
GTAATGACTTGCGTTTACTTTCTTCTGGCCCTCGTGCAGGTTTAAAAGAAATTAATCTTCCTGAATTACAAGCGGGTTCT
TCTATTATGCCAGCAAAAGTAAACCCAGTTGTTCCAGAGGTGGTAAACCAAGTGTGCTTTAAAGTAATTGGTAACGATAC
CACTGTGACTTTCGCATCTGAAGCAGGTCAATTACAATTAAACGTAATGGAACCAGTGATTGGTCAAGCAATGTTCGAAT
CTATCGACATCTTAACCAATGCTTGTGTGAACTTACGCGATAAATGCGTGGATGGCATCACTGTAAACAAAGAAATTTGT
GAAAACTACGTGTTTAATTCAATTGGTATTGTGACTTACTTAAATCCATTTATTGGTCACCACAACGGCGACTTAGTGGG
TAAAATCTGTGCACAAACAGGCAAAGGCGTACGTGAAGTTGTGTTAGAAAAAGGTTTATTAACAGAAGAACAATTAGATG
ACATTCTTTCTGTAGAAAACTTAATGAATCCAACTTACAAAGCGAAATTAAATAAATAA

Upstream 100 bases:

>100_bases
GTCTTGAGAGCATTTTTTGAAAATGTGATCTTCATCAAGTTTTTTCTTCTTGAAATTTCTATAATGACGATAAATTAACT
AACCCAAAAAGGTAATTAAA

Downstream 100 bases:

>100_bases
TTTACGCCAAATATCAAAAAAATTTACCGCACTTTAATCTGTGCCTTAAAGCTGAATAAATAAACCAGCTAATTGGGGCG
CAGATTTTTTTTTATGATTA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTQFRKEVDLLGERDVPAEAYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALANGELGAIPSDIAKAIVAACD
EILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALELLGHKKGEYQYLDPMDHVNASQSTNDAYPTGFRIAVYNSIL
KLIDKIQYLHDGFDNKAKEFANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEVNLGATAIGTGLNT
PQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLKEINLPELQAGS
SIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQLNVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNKEIC
ENYVFNSIGIVTYLNPFIGHHNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK

Sequences:

>Translated_472_residues
MTQFRKEVDLLGERDVPAEAYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALANGELGAIPSDIAKAIVAACD
EILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALELLGHKKGEYQYLDPMDHVNASQSTNDAYPTGFRIAVYNSIL
KLIDKIQYLHDGFDNKAKEFANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEVNLGATAIGTGLNT
PQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLKEINLPELQAGS
SIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQLNVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNKEIC
ENYVFNSIGIVTYLNPFIGHHNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK
>Mature_471_residues
TQFRKEVDLLGERDVPAEAYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALANGELGAIPSDIAKAIVAACDE
ILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALELLGHKKGEYQYLDPMDHVNASQSTNDAYPTGFRIAVYNSILK
LIDKIQYLHDGFDNKAKEFANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEVNLGATAIGTGLNTP
QGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLKEINLPELQAGSS
IMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQLNVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNKEICE
NYVFNSIGIVTYLNPFIGHHNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=40.6926406926407, Blast_Score=334, Evalue=1e-91,
Organism=Escherichia coli, GI87082375, Length=466, Percent_Identity=79.3991416309013, Blast_Score=762, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=37.7969762419007, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1790398, Length=282, Percent_Identity=23.0496453900709, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17553882, Length=465, Percent_Identity=40.8602150537634, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=41.1042944785276, Blast_Score=257, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6324993, Length=464, Percent_Identity=40.301724137931, Blast_Score=339, Evalue=4e-94,
Organism=Drosophila melanogaster, GI24640177, Length=465, Percent_Identity=40.4301075268817, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24640179, Length=465, Percent_Identity=40.4301075268817, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=38.7931034482759, Blast_Score=331, Evalue=7e-91,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=39.4396551724138, Blast_Score=331, Evalue=7e-91,
Organism=Drosophila melanogaster, GI24583245, Length=449, Percent_Identity=33.184855233853, Blast_Score=270, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51173; Mature: 51041

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFRKEVDLLGERDVPAEAYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHC
NGELGAIPSDIAKAIVAACDEILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCHHHHHHHHHHHH
LGHKKGEYQYLDPMDHVNASQSTNDAYPTGFRIAVYNSILKLIDKIQYLHDGFDNKAKEF
HCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEVNLGATAIGTGLNT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEECCCCCC
PQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLL
CCCHHHHHHHHHHHHHCCEECCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SSGPRAGLKEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
NVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNKEICENYVFNSIGIVTYLNPFIGH
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
HNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK
CCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHCCCCC
>Mature Secondary Structure 
TQFRKEVDLLGERDVPAEAYWGIHTLRAVENFNISNVTISDVPEFVRGMVMVKKATALA
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHC
NGELGAIPSDIAKAIVAACDEILTTGKCLDQFPSDVYQGGAGTSVNMNTNEVVANLALEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCHHHHHHHHHHHH
LGHKKGEYQYLDPMDHVNASQSTNDAYPTGFRIAVYNSILKLIDKIQYLHDGFDNKAKEF
HCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ANILKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAGLLLEVNLGATAIGTGLNT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEECCCCCC
PQGYTELVVKHLAEVTGLACVPAENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLL
CCCHHHHHHHHHHHHHCCEECCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SSGPRAGLKEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFASEAGQLQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
NVMEPVIGQAMFESIDILTNACVNLRDKCVDGITVNKEICENYVFNSIGIVTYLNPFIGH
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
HNGDLVGKICAQTGKGVREVVLEKGLLTEEQLDDILSVENLMNPTYKAKLNK
CCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]