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The map label for this gene is rpoD [H]

Identifier: 148827724

GI number: 148827724

Start: 1079963

End: 1081852

Strand: Direct

Name: rpoD [H]

Synonym: CGSHiGG_05915

Alternate gene names: 148827724

Gene position: 1079963-1081852 (Clockwise)

Preceding gene: 148827723

Following gene: 148827725

Centisome position: 57.23

GC content: 37.72

Gene sequence:

>1890_bases
ATGGAAAAAAATCAACAATCTACGGCTGAACAATATTCAGAACAAATTGAGCAATTGATGGAGCTAGGTCGTACACAAGG
GTATTTAACTTTTGCTGAAATCAATGACTTATTGCCCGAAGATGCTATTGATCCTGAATATTACGACAAATTGCTACAAA
CGTTGCAAAATGATGCAGGTATCCCTGTATTAGATGAAGCACCAGAAAGCGATGATATGATGTTGAGTGATACCATCCCT
GATGAAGATGCAGTGGAAGAAGCAACTCAAATTCTTTCAAATGTAGAATCTGAAATAGGTCGAACAACGGATCCCGTACG
CATGTATATGCGCGAAATGGGAACGGTGGATCTTCTTACTCGTGAAGATGAAATTAGCATTGCAAAACGTATTGAAGGAG
GGATTGATGAAGTTCAAACCTCTATCGCAGCCTATCCTGAAGCATTAAATGGATTACTAAAAAATTATGATGATGTAGAA
AAAGGTAATTTCCGTTTGACCGATTTAATTACGGGTTTTGTTGATCCAAATGCCGAAATAGAAGAACACAACGGACTTGA
TGAAGACTTTTCTGACGAAGATGATGAAGAAGAATCTAGTAATGCAGATGTGGAAGACAATGAAGATGAAGAAGACAATG
AATCAGAATCTACATCAGATTCAAGCGACTCTGATAACAGTATTGATCCTGAAGTTGCTCGCGAAAAATTTCAGCAATTA
AGAGAACAGCACTCTAAAACCCTTGCTGTTATTGAAAAACACGGTCGCTCTGGAAAACGTGCCCAAGATCAAATTGCACT
ACTTGGCGAAATTTTTAAACAATTCCGTTTAGTACCAAAACAATTTGATTTACTCGTTCTATCAATGAAAGAAATGATGA
AACGTGTGCGCTACCAAGAACGTCAGCTACAAAAAATATTAGTGGATATAGCAGGAATGCCAAAGGATGATTTTGAAAAA
ATTATTACCGCTAATGGCAGTAACAGTGAATGGGTAGCAAAAGCATTAAAATCTAGCAAACCTTGGGCTAAACGTTTAAT
TAAATATGAAGATCGTATTTATGAAGCCTTAAACAATCTAGCGATAACAGAAGAAAATACTAAACTTACCATTACTCAAA
TGCGTGACATTTGCGATGCAGTCGCACGAGGCGAACAAAAAGCACGTCGAGCTAAAAAAGAAATGGTTGAAGCTAACTTG
CGTTTAGTAATTTCCATCGCGAAAAAATACACAAATCGAGGTTTACAATTCTTAGATTTAATTCAAGAAGGGAATATCGG
TTTAATGAAAGCGGTAGATAAATTTGAATATCGACGAGGCTACAAATTCTCTACATATGCTACTTGGTGGATTCGTCAAG
CAATCACACGCTCTATTGCGGATCAAGCGCGTACAATCCGTATTCCTGTACATATGATTGAAACAATCAATAAACTTAAT
CGTATTTCTCGCCAGTTACTACAAGAAATGGGACGGGAAGCAACACCAGAAGAATTGGCAGAACGTATGGGAATGCCAGA
AGATAAAATCCGTAAAGTACTGAAAATTGCAAAAGAGCCGATTTCTATGGAAACACCTATTGGCGATGACGATGATTCGC
ATTTAGGGGATTTTATTGAAGATTCAACCTTAGAACTTCCTCTTGATTCTGCCACAGCACAAAGTTTAAAAGTTGCAACT
CACGAAGTGCTAGAAGGCTTGACGCCACGTGAAGCAAAAGTATTACGTATGCGTTTTGGCATTGATATGAATACCGACCA
TACACTTGAAGAAGTGGGTAAACAATTCGATGTAACACGCGAACGTATTCGTCAAATTGAAGCAAAAGCATTACGTAAAT
TACGTCATCCTAGCCGTTCTGAAACACTGAGAAGTTTTTTAGATGAATAG

Upstream 100 bases:

>100_bases
GCAGAAAAAACAGTTAGTGAATCCGCTATAACAATGGTAGAATCTCGGGTTCATAAAAAATCTTATCTTCTCAATCACAT
CAAGCAAAACGAGTATAAAA

Downstream 100 bases:

>100_bases
AGAGAAACATAAAAGAGAAAAATCGATCTAATCTTAAAAAATCAGATACATTTGTTTAAGGACTAAATTCTATAATCCGC
AGAATTTAGTCCTTTTACTT

Product: DNA primase

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MEKNQQSTAEQYSEQIEQLMELGRTQGYLTFAEINDLLPEDAIDPEYYDKLLQTLQNDAGIPVLDEAPESDDMMLSDTIP
DEDAVEEATQILSNVESEIGRTTDPVRMYMREMGTVDLLTREDEISIAKRIEGGIDEVQTSIAAYPEALNGLLKNYDDVE
KGNFRLTDLITGFVDPNAEIEEHNGLDEDFSDEDDEEESSNADVEDNEDEEDNESESTSDSSDSDNSIDPEVAREKFQQL
REQHSKTLAVIEKHGRSGKRAQDQIALLGEIFKQFRLVPKQFDLLVLSMKEMMKRVRYQERQLQKILVDIAGMPKDDFEK
IITANGSNSEWVAKALKSSKPWAKRLIKYEDRIYEALNNLAITEENTKLTITQMRDICDAVARGEQKARRAKKEMVEANL
RLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLN
RISRQLLQEMGREATPEELAERMGMPEDKIRKVLKIAKEPISMETPIGDDDDSHLGDFIEDSTLELPLDSATAQSLKVAT
HEVLEGLTPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDE

Sequences:

>Translated_629_residues
MEKNQQSTAEQYSEQIEQLMELGRTQGYLTFAEINDLLPEDAIDPEYYDKLLQTLQNDAGIPVLDEAPESDDMMLSDTIP
DEDAVEEATQILSNVESEIGRTTDPVRMYMREMGTVDLLTREDEISIAKRIEGGIDEVQTSIAAYPEALNGLLKNYDDVE
KGNFRLTDLITGFVDPNAEIEEHNGLDEDFSDEDDEEESSNADVEDNEDEEDNESESTSDSSDSDNSIDPEVAREKFQQL
REQHSKTLAVIEKHGRSGKRAQDQIALLGEIFKQFRLVPKQFDLLVLSMKEMMKRVRYQERQLQKILVDIAGMPKDDFEK
IITANGSNSEWVAKALKSSKPWAKRLIKYEDRIYEALNNLAITEENTKLTITQMRDICDAVARGEQKARRAKKEMVEANL
RLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLN
RISRQLLQEMGREATPEELAERMGMPEDKIRKVLKIAKEPISMETPIGDDDDSHLGDFIEDSTLELPLDSATAQSLKVAT
HEVLEGLTPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDE
>Mature_629_residues
MEKNQQSTAEQYSEQIEQLMELGRTQGYLTFAEINDLLPEDAIDPEYYDKLLQTLQNDAGIPVLDEAPESDDMMLSDTIP
DEDAVEEATQILSNVESEIGRTTDPVRMYMREMGTVDLLTREDEISIAKRIEGGIDEVQTSIAAYPEALNGLLKNYDDVE
KGNFRLTDLITGFVDPNAEIEEHNGLDEDFSDEDDEEESSNADVEDNEDEEDNESESTSDSSDSDNSIDPEVAREKFQQL
REQHSKTLAVIEKHGRSGKRAQDQIALLGEIFKQFRLVPKQFDLLVLSMKEMMKRVRYQERQLQKILVDIAGMPKDDFEK
IITANGSNSEWVAKALKSSKPWAKRLIKYEDRIYEALNNLAITEENTKLTITQMRDICDAVARGEQKARRAKKEMVEANL
RLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLN
RISRQLLQEMGREATPEELAERMGMPEDKIRKVLKIAKEPISMETPIGDDDDSHLGDFIEDSTLELPLDSATAQSLKVAT
HEVLEGLTPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=629, Percent_Identity=67.2496025437202, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=241, Percent_Identity=41.9087136929461, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=28.9256198347107, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788231, Length=212, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 72040; Mature: 72040

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNQQSTAEQYSEQIEQLMELGRTQGYLTFAEINDLLPEDAIDPEYYDKLLQTLQNDAG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
IPVLDEAPESDDMMLSDTIPDEDAVEEATQILSNVESEIGRTTDPVRMYMREMGTVDLLT
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHEEC
REDEISIAKRIEGGIDEVQTSIAAYPEALNGLLKNYDDVEKGNFRLTDLITGFVDPNAEI
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCCC
EEHNGLDEDFSDEDDEEESSNADVEDNEDEEDNESESTSDSSDSDNSIDPEVAREKFQQL
HHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
REQHSKTLAVIEKHGRSGKRAQDQIALLGEIFKQFRLVPKQFDLLVLSMKEMMKRVRYQE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RQLQKILVDIAGMPKDDFEKIITANGSNSEWVAKALKSSKPWAKRLIKYEDRIYEALNNL
HHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
AITEENTKLTITQMRDICDAVARGEQKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDL
EEECCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLN
HHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHH
RISRQLLQEMGREATPEELAERMGMPEDKIRKVLKIAKEPISMETPIGDDDDSHLGDFIE
HHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHC
DSTLELPLDSATAQSLKVATHEVLEGLTPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTR
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ERIRQIEAKALRKLRHPSRSETLRSFLDE
HHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MEKNQQSTAEQYSEQIEQLMELGRTQGYLTFAEINDLLPEDAIDPEYYDKLLQTLQNDAG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
IPVLDEAPESDDMMLSDTIPDEDAVEEATQILSNVESEIGRTTDPVRMYMREMGTVDLLT
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHEEC
REDEISIAKRIEGGIDEVQTSIAAYPEALNGLLKNYDDVEKGNFRLTDLITGFVDPNAEI
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCCC
EEHNGLDEDFSDEDDEEESSNADVEDNEDEEDNESESTSDSSDSDNSIDPEVAREKFQQL
HHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
REQHSKTLAVIEKHGRSGKRAQDQIALLGEIFKQFRLVPKQFDLLVLSMKEMMKRVRYQE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RQLQKILVDIAGMPKDDFEKIITANGSNSEWVAKALKSSKPWAKRLIKYEDRIYEALNNL
HHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
AITEENTKLTITQMRDICDAVARGEQKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDL
EEECCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLN
HHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHH
RISRQLLQEMGREATPEELAERMGMPEDKIRKVLKIAKEPISMETPIGDDDDSHLGDFIE
HHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHC
DSTLELPLDSATAQSLKVATHEVLEGLTPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTR
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ERIRQIEAKALRKLRHPSRSETLRSFLDE
HHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]