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The map label for this gene is dnaG [H]

Identifier: 148827723

GI number: 148827723

Start: 1078112

End: 1079893

Strand: Direct

Name: dnaG [H]

Synonym: CGSHiGG_05910

Alternate gene names: 148827723

Gene position: 1078112-1079893 (Clockwise)

Preceding gene: 148827722

Following gene: 148827724

Centisome position: 57.13

GC content: 39.56

Gene sequence:

>1782_bases
ATGAAAGGTTCTATTCCACGCCCCTTTATTGATGATTTGCTGACAAAGTCCGATATTGTCGATGTGATTAACACGCGCGT
AAAACTAAAAAAAGCTGGCCGCGATTATCAAGCCTGCTGCCCTTTCCATCACGAAAAAACACCATCTTTCACAGTTAGCC
AAAAGAAACAGTTTTATCATTGCTTTGGTTGTGGCGCACATGGTAATGCGATTTCCTTTTTAATGGACTATGACAAACTT
GAATTTATAGAGGCGATTGAAGAACTTGCAGCTATGGCAGGGCTTGAAATACCTTACGAAAAACGCGCTAATCACAGCGG
GAAACCTCAAGCTAATTACCAAACCAAACGAAATCTCTATGAATTAATGCAAGAGATTGCCAAGTTTTATCAAAACCAAT
TACCATTAAATACTCAAGCACAAGAATATTTACAACAACGTGGGCTTTCACCTGAAATTATTGAGCGTTTCCAAATTGGG
TTTGTGCCGAATGCAATGGATACTGTACTGCGTAAATTTGGTGTTAATCGTGAAGAACAACAAAAACTCATTGAGTTAGG
CATGCTTTCTCGAAATGATCGTGGCAATATTTACGATAAATTCCGAAATCGCATCATGTTTCCAATTCGCGATAAACGTG
GGCGTACAGTGGCTTTTGGTGGGCGTGTATTAACAGATGAAAAACCGAAATATCTGAATTCGCCAGAAACCATTACTTAT
CATAAAGGTAAGGAGCTTTATGGTTTGTATGAAGCCTTACAAACCAACGATGAACCCAAACAATTACTTGTTGTTGAAGG
TTATATGGATGTAGTGGCATTAGCACAATTTGGCGTGGATTATGCGGTCGCTTCTTTAGGTACGTCCACGACATCAGAAC
AAATTCAACTTATTTTTCGTTCGACCGAGCAAGTTGTTTGTTGTTACGATGGTGACCGTGCAGGGCGTGATGCTGCTTGG
CGCGCACTAGAAAATGCGTTGCCCTATTTAGAAGATGGCAGACAACTCAAATTTATTTTCTTGCCAGATGGAGAAGATCC
TGATACTTACATTCGTCAATACGGCAAAGAAAAATTTGAAGAATACATCGAAAGTGCGCAATCTTTAAGCGAGTTTATGT
TTGCACATTTAAGTCCTCAGGTTGATTTTTCCACTAAAGAAGGGCGCGGAAAATTAGTTGCACTAGCTGCGCCGTTAATT
CACCAAATTCCAGGAGAAATGCTTCGCTTATCGCTACGCAATATGCTTGCTCAAAAACTTGGGATTTTTGATCAAACTCA
GCTGGAAAATCTTATTCCCAAAAAATTAGAACAAGCCAATACACAACAAAAAGTCACTCATAATAAGATCAAGAAGACAC
CAATGCGAATGGTCATTTCATTGCTTCTGCAAAATCCTGAGTTAGTAAAACGTATGTCTGAAAGCGGCGTGCAAGCATTA
CGTGCAGAAGCAGGGTTTGAAATTCTGGAAAAATTGACCGCACTTTGTCGCCAACGAGAGGGCATTACTACGGGGCAAAT
TTTGGAATACTTCCGCAATACATCTTACAGTAATCCCCTTGAAATACTGGCAACTTGGGATCATTTATTAGACGAATCCG
ACATAATCAACGCATTTTCTCAAAATTACCGTCGCTTAAATATTCAAGCTATTGAGCGCGATATTGAAATGCTTATTGCT
AAAGAACGAACAGAGGGTCTCACTAATGAAGAAAAAACGGTACTTGTACATCTTTTAGCTGGTAAAGAACAGCAGAAAAA
ACAGTTAGTGAATCCGCTATAA

Upstream 100 bases:

>100_bases
CCGTGAATCCTTTGGACTCACGGTTTTGTTACTTTAAGCACAATAAAAATCTACGCCAAAAACGACCGCACTTTCACACC
ACGATCACGGAGGCTCGACA

Downstream 100 bases:

>100_bases
CAATGGTAGAATCTCGGGTTCATAAAAAATCTTATCTTCTCAATCACATCAAGCAAAACGAGTATAAAAATGGAAAAAAA
TCAACAATCTACGGCTGAAC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MKGSIPRPFIDDLLTKSDIVDVINTRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYHCFGCGAHGNAISFLMDYDKL
EFIEAIEELAAMAGLEIPYEKRANHSGKPQANYQTKRNLYELMQEIAKFYQNQLPLNTQAQEYLQQRGLSPEIIERFQIG
FVPNAMDTVLRKFGVNREEQQKLIELGMLSRNDRGNIYDKFRNRIMFPIRDKRGRTVAFGGRVLTDEKPKYLNSPETITY
HKGKELYGLYEALQTNDEPKQLLVVEGYMDVVALAQFGVDYAVASLGTSTTSEQIQLIFRSTEQVVCCYDGDRAGRDAAW
RALENALPYLEDGRQLKFIFLPDGEDPDTYIRQYGKEKFEEYIESAQSLSEFMFAHLSPQVDFSTKEGRGKLVALAAPLI
HQIPGEMLRLSLRNMLAQKLGIFDQTQLENLIPKKLEQANTQQKVTHNKIKKTPMRMVISLLLQNPELVKRMSESGVQAL
RAEAGFEILEKLTALCRQREGITTGQILEYFRNTSYSNPLEILATWDHLLDESDIINAFSQNYRRLNIQAIERDIEMLIA
KERTEGLTNEEKTVLVHLLAGKEQQKKQLVNPL

Sequences:

>Translated_593_residues
MKGSIPRPFIDDLLTKSDIVDVINTRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYHCFGCGAHGNAISFLMDYDKL
EFIEAIEELAAMAGLEIPYEKRANHSGKPQANYQTKRNLYELMQEIAKFYQNQLPLNTQAQEYLQQRGLSPEIIERFQIG
FVPNAMDTVLRKFGVNREEQQKLIELGMLSRNDRGNIYDKFRNRIMFPIRDKRGRTVAFGGRVLTDEKPKYLNSPETITY
HKGKELYGLYEALQTNDEPKQLLVVEGYMDVVALAQFGVDYAVASLGTSTTSEQIQLIFRSTEQVVCCYDGDRAGRDAAW
RALENALPYLEDGRQLKFIFLPDGEDPDTYIRQYGKEKFEEYIESAQSLSEFMFAHLSPQVDFSTKEGRGKLVALAAPLI
HQIPGEMLRLSLRNMLAQKLGIFDQTQLENLIPKKLEQANTQQKVTHNKIKKTPMRMVISLLLQNPELVKRMSESGVQAL
RAEAGFEILEKLTALCRQREGITTGQILEYFRNTSYSNPLEILATWDHLLDESDIINAFSQNYRRLNIQAIERDIEMLIA
KERTEGLTNEEKTVLVHLLAGKEQQKKQLVNPL
>Mature_593_residues
MKGSIPRPFIDDLLTKSDIVDVINTRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYHCFGCGAHGNAISFLMDYDKL
EFIEAIEELAAMAGLEIPYEKRANHSGKPQANYQTKRNLYELMQEIAKFYQNQLPLNTQAQEYLQQRGLSPEIIERFQIG
FVPNAMDTVLRKFGVNREEQQKLIELGMLSRNDRGNIYDKFRNRIMFPIRDKRGRTVAFGGRVLTDEKPKYLNSPETITY
HKGKELYGLYEALQTNDEPKQLLVVEGYMDVVALAQFGVDYAVASLGTSTTSEQIQLIFRSTEQVVCCYDGDRAGRDAAW
RALENALPYLEDGRQLKFIFLPDGEDPDTYIRQYGKEKFEEYIESAQSLSEFMFAHLSPQVDFSTKEGRGKLVALAAPLI
HQIPGEMLRLSLRNMLAQKLGIFDQTQLENLIPKKLEQANTQQKVTHNKIKKTPMRMVISLLLQNPELVKRMSESGVQAL
RAEAGFEILEKLTALCRQREGITTGQILEYFRNTSYSNPLEILATWDHLLDESDIINAFSQNYRRLNIQAIERDIEMLIA
KERTEGLTNEEKTVLVHLLAGKEQQKKQLVNPL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=580, Percent_Identity=56.2068965517241, Blast_Score=673, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68003; Mature: 68003

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGSIPRPFIDDLLTKSDIVDVINTRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHH
CFGCGAHGNAISFLMDYDKLEFIEAIEELAAMAGLEIPYEKRANHSGKPQANYQTKRNLY
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHH
ELMQEIAKFYQNQLPLNTQAQEYLQQRGLSPEIIERFQIGFVPNAMDTVLRKFGVNREEQ
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
QKLIELGMLSRNDRGNIYDKFRNRIMFPIRDKRGRTVAFGGRVLTDEKPKYLNSPETITY
HHHHHHHHHCCCCCCCHHHHHHCCEECEEECCCCCEEEECCEEECCCCCCCCCCCCEEEE
HKGKELYGLYEALQTNDEPKQLLVVEGYMDVVALAQFGVDYAVASLGTSTTSEQIQLIFR
ECCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
STEQVVCCYDGDRAGRDAAWRALENALPYLEDGRQLKFIFLPDGEDPDTYIRQYGKEKFE
CCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
EYIESAQSLSEFMFAHLSPQVDFSTKEGRGKLVALAAPLIHQIPGEMLRLSLRNMLAQKL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHH
GIFDQTQLENLIPKKLEQANTQQKVTHNKIKKTPMRMVISLLLQNPELVKRMSESGVQAL
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RAEAGFEILEKLTALCRQREGITTGQILEYFRNTSYSNPLEILATWDHLLDESDIINAFS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHH
QNYRRLNIQAIERDIEMLIAKERTEGLTNEEKTVLVHLLAGKEQQKKQLVNPL
HHHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCCC
>Mature Secondary Structure
MKGSIPRPFIDDLLTKSDIVDVINTRVKLKKAGRDYQACCPFHHEKTPSFTVSQKKQFYH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHH
CFGCGAHGNAISFLMDYDKLEFIEAIEELAAMAGLEIPYEKRANHSGKPQANYQTKRNLY
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHH
ELMQEIAKFYQNQLPLNTQAQEYLQQRGLSPEIIERFQIGFVPNAMDTVLRKFGVNREEQ
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
QKLIELGMLSRNDRGNIYDKFRNRIMFPIRDKRGRTVAFGGRVLTDEKPKYLNSPETITY
HHHHHHHHHCCCCCCCHHHHHHCCEECEEECCCCCEEEECCEEECCCCCCCCCCCCEEEE
HKGKELYGLYEALQTNDEPKQLLVVEGYMDVVALAQFGVDYAVASLGTSTTSEQIQLIFR
ECCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
STEQVVCCYDGDRAGRDAAWRALENALPYLEDGRQLKFIFLPDGEDPDTYIRQYGKEKFE
CCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
EYIESAQSLSEFMFAHLSPQVDFSTKEGRGKLVALAAPLIHQIPGEMLRLSLRNMLAQKL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHH
GIFDQTQLENLIPKKLEQANTQQKVTHNKIKKTPMRMVISLLLQNPELVKRMSESGVQAL
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RAEAGFEILEKLTALCRQREGITTGQILEYFRNTSYSNPLEILATWDHLLDESDIINAFS
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHH
QNYRRLNIQAIERDIEMLIAKERTEGLTNEEKTVLVHLLAGKEQQKKQLVNPL
HHHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8294018; 7542800; 8316085 [H]