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The map label for this gene is pgk [H]

Identifier: 148827716

GI number: 148827716

Start: 1072130

End: 1073290

Strand: Reverse

Name: pgk [H]

Synonym: CGSHiGG_05875

Alternate gene names: 148827716

Gene position: 1073290-1072130 (Counterclockwise)

Preceding gene: 148827717

Following gene: 148827715

Centisome position: 56.87

GC content: 39.62

Gene sequence:

>1161_bases
ATGTCAGTAATCAAAATGACCGATTTAGATTTAGCGGGTAAACGTGTATTTATCCGTGCGGATCTTAATGTGCCAGTAAA
AGATGGCAAAGTGACCTCAGATGCGCGTATTCGTGCGACCATTCCTACTTTAAAATTAGCCTTAGAAAAAGGTGCGAAAG
TGATGGTAACTTCTCACTTAGGTCGTCCAACTGAAGGAGAATTCAAACCAGAAGATTCTTTACAACCAGTTGTTGATTAC
TTAAAAAACGCAGGCTTTAATGTGCGTTTAGAACAAGATTATTTAAATGGTGTGGATGTTAAAGATGGCGAAATCGTTGT
TTTAGAAAACGTACGTGTAAACAAAGGCGAAAAGAAAAATGATCCAGAATTAGGTAAAAAATATGCTGCACTTTGCGACG
TATTTGTAATGGATGCCTTTGGTACAGCGCACCGCGCGCAAGCATCAACTTATGGCGTTGCAGAATTTGCGCCAATCGCT
TGTGCAGGCCCATTATTAGCGGCTGAGTTAGATGCATTAGGTAAAGCATTAAAAGAACCTGCTCGTCCAATGGTGGCTAT
TGTTGGTGGTTCTAAAGTTTCCACTAAATTAGAAGTATTAAATTCTCTTTCAAAAATCGCTGACCAAATTATTGTGGGCG
GTGGCATTGCGAATACTTTCATTGCGGCAGCTGGTCACAATGTAGGTAAATCTTTATATGAAGCTGATTTAATTCCAGTT
GCGAAAGAATTAGCCGCAAATACAGATATTCCAGTTCCAGTTGATGTGCGTGTAGGTTTAGAGTTCTCTGAAACGGCAAC
AGCAACAGAAAAAGCGGTAAATGAAGTGAAAGATGATGAATCTATTTTTGATATTGGCGATAAATCAGCAGAACAATTGG
CTGAAATTATCAAAAATGCAAAAACCGTTTTGTGGAATGGTCCAGTTGGTGTGTTTGAGTTCCCTCACTTCCGTAAAGGG
ACTGAAATCATTTCTCACGCCATTGCAAACAGCGATGCATTCTCTATTGCTGGCGGCGGCGATACTTTAGCGGCTATCGA
TTTATTTGGTATTGCTGATAAGATTTCTTACATTTCAACGGGTGGTGGTGCATTCTTAGAATTTGTAGAAGGCAAAGTAT
TACCTGCAGTAGAAATTCTTGAAAAACGTGCGAAAAACTAA

Upstream 100 bases:

>100_bases
AATAAGAATTTCTTTGATCTAAATCGTTACAACCTAGCACGAAGTAGGTATAATACGAGAAGTTTTCAATTTTAATTTTA
ACTTAACAAGAGGATAGATT

Downstream 100 bases:

>100_bases
CCGCACTTTTCCTCTATAACATATTGATGATGGCATCAAAAATGCCATCATCAATACAAATTCTTCAAATTGTGGGCTTA
TCCCACTAAACATAAAAAGG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHLGRPTEGEFKPEDSLQPVVDY
LKNAGFNVRLEQDYLNGVDVKDGEIVVLENVRVNKGEKKNDPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEFAPIA
CAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEADLIPV
AKELAANTDIPVPVDVRVGLEFSETATATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNAKTVLWNGPVGVFEFPHFRKG
TEIISHAIANSDAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVEILEKRAKN

Sequences:

>Translated_386_residues
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHLGRPTEGEFKPEDSLQPVVDY
LKNAGFNVRLEQDYLNGVDVKDGEIVVLENVRVNKGEKKNDPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEFAPIA
CAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEADLIPV
AKELAANTDIPVPVDVRVGLEFSETATATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNAKTVLWNGPVGVFEFPHFRKG
TEIISHAIANSDAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVEILEKRAKN
>Mature_385_residues
SVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHLGRPTEGEFKPEDSLQPVVDYL
KNAGFNVRLEQDYLNGVDVKDGEIVVLENVRVNKGEKKNDPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEFAPIAC
AGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEADLIPVA
KELAANTDIPVPVDVRVGLEFSETATATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNAKTVLWNGPVGVFEFPHFRKGT
EIISHAIANSDAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVEILEKRAKN

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=412, Percent_Identity=36.8932038834951, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=36.2530413625304, Blast_Score=234, Evalue=1e-61,
Organism=Escherichia coli, GI1789294, Length=386, Percent_Identity=81.3471502590674, Blast_Score=626, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=40.1937046004843, Blast_Score=253, Evalue=8e-68,
Organism=Saccharomyces cerevisiae, GI6319857, Length=419, Percent_Identity=38.1861575178998, Blast_Score=233, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=38.2566585956416, Blast_Score=237, Evalue=9e-63,
Organism=Drosophila melanogaster, GI17136394, Length=410, Percent_Identity=38.780487804878, Blast_Score=234, Evalue=9e-62,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 40985; Mature: 40854

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHL
CCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCEEEEEECC
GRPTEGEFKPEDSLQPVVDYLKNAGFNVRLEQDYLNGVDVKDGEIVVLENVRVNKGEKKN
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCEEEEECEEECCCCCCC
DPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEFAPIACAGPLLAAELDALGKALKEP
CHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHCCHHHCCHHHHHHHHHHHHHHHCC
ARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEADLIPV
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCHHHHHHHHHHHHHH
AKELAANTDIPVPVDVRVGLEFSETATATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNA
HHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHCC
KTVLWNGPVGVFEFPHFRKGTEIISHAIANSDAFSIAGGGDTLAAIDLFGIADKISYIST
CEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHEEC
GGGAFLEFVEGKVLPAVEILEKRAKN
CCHHHHHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHL
CEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCEEEEEECC
GRPTEGEFKPEDSLQPVVDYLKNAGFNVRLEQDYLNGVDVKDGEIVVLENVRVNKGEKKN
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCEEEEECEEECCCCCCC
DPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEFAPIACAGPLLAAELDALGKALKEP
CHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHCCHHHCCHHHHHHHHHHHHHHHCC
ARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEADLIPV
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCHHHHHHHHHHHHHH
AKELAANTDIPVPVDVRVGLEFSETATATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNA
HHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHCC
KTVLWNGPVGVFEFPHFRKGTEIISHAIANSDAFSIAGGGDTLAAIDLFGIADKISYIST
CEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHEEC
GGGAFLEFVEGKVLPAVEILEKRAKN
CCHHHHHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]