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The map label for this gene is potD-A [H]

Identifier: 148827692

GI number: 148827692

Start: 1040019

End: 1041071

Strand: Reverse

Name: potD-A [H]

Synonym: CGSHiGG_05745

Alternate gene names: 148827692

Gene position: 1041071-1040019 (Counterclockwise)

Preceding gene: 148827701

Following gene: 148827690

Centisome position: 55.17

GC content: 36.94

Gene sequence:

>1053_bases
TTGAAAAAATTCTTTGCTCACTCACTCAAAAATCTTCTCCTTTCCACCACCGCACTTTTTGCGGCTTCCGCTTTTGCCAA
TAACAAACTTTACGTCTATAACTGGACAGACTATGTGCCTTCTGATTTAGTCGCACAGTTCTCTAAAGAAACTGGCATCG
AGGTAATTTATTCTACCTTTGAAAGTAACGAAGAAATGTATGCGAAACTTAAACTTACTCAGAATACTGGTTCTGGCTAT
GATTTGGTGTTTCCATCAAGTTACTACGTAAATAAAATGATTAAGGAAAAGATGCTTCAACCGATTGATCAAAGTAAATT
GACCAATATTCATCAAATCCCTAAACATTTATTAAATAAAGAATTCGACCCAGAAAATAAATATTCATTACCTTACGTTT
ACGGCTTAACAGGTATTGAAGTTAATGCGGATGAGATTGATCCTAAAACCATCACTAGCTGGGCGGATTTATGGAAACCT
GAATTTAAAGGCAAAGTTCTCATGACCAGTGATGCGCGTGAAGTGTTCCACGTAGCATTATTACTGGATGGTAAATCGCC
AAATACCACCAATGAAGAAGACATCAAAACAGCTTATGAGCGTTTAGAAAAACTGTTACCAAATGTAGCGACTTTTAACT
CTGATTCTCCTGAAGTCCCTTACGTACAAGGCGAAGTTGCAATTGGTATGATTTGGAATGGTTCAGCTTATTTAGCGCAG
AAAGAAAATCAATCTCTTCAATTTGTGTATCCAAAAGAAGGCGCAATTTTCTGGATGGATAACTATGCGATTCCGACTAC
AGCAAAAAATGTTGAAGGTGCACATAAGTTCATCGACTTTTTACTTCGCCCTGAAAATGCAAAAATTGTTATCGAACGCA
TGGGCTTTTCTATGCCAAATAATGGGGCGAAAACATTGCTAAGCGCGGAAGTTGCTAATGATCCAAAATTATTCCCACCA
GCGGAAGAAGTAGAAAAAGGAATTATGCAAGGCGATGTAGGAGAAGCTGTTGATATTTATGAAAAATATTGGAGTAAATT
AAAAACTAATTAG

Upstream 100 bases:

>100_bases
AAAGGGAAATATGTCAAACCTTTTAGAAAAAAATGCTATACTGCGCGCGCGCAAAATTTGCGTGTTTTATGATGAACGAT
AACCCTCGGAGGTCAATAAA

Downstream 100 bases:

>100_bases
TTTCTCTATCCTGAAAACAAAAAAGTGCGGTAAAAATTTACCGCACTTTTATATGTAAAATTTTTATCTTTAAAGAATAA
AACGGCTCAAATCTTCATTT

Product: spermidine/putrescine ABC transporter periplasmic-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKKFFAHSLKNLLLSTTALFAASAFANNKLYVYNWTDYVPSDLVAQFSKETGIEVIYSTFESNEEMYAKLKLTQNTGSGY
DLVFPSSYYVNKMIKEKMLQPIDQSKLTNIHQIPKHLLNKEFDPENKYSLPYVYGLTGIEVNADEIDPKTITSWADLWKP
EFKGKVLMTSDAREVFHVALLLDGKSPNTTNEEDIKTAYERLEKLLPNVATFNSDSPEVPYVQGEVAIGMIWNGSAYLAQ
KENQSLQFVYPKEGAIFWMDNYAIPTTAKNVEGAHKFIDFLLRPENAKIVIERMGFSMPNNGAKTLLSAEVANDPKLFPP
AEEVEKGIMQGDVGEAVDIYEKYWSKLKTN

Sequences:

>Translated_350_residues
MKKFFAHSLKNLLLSTTALFAASAFANNKLYVYNWTDYVPSDLVAQFSKETGIEVIYSTFESNEEMYAKLKLTQNTGSGY
DLVFPSSYYVNKMIKEKMLQPIDQSKLTNIHQIPKHLLNKEFDPENKYSLPYVYGLTGIEVNADEIDPKTITSWADLWKP
EFKGKVLMTSDAREVFHVALLLDGKSPNTTNEEDIKTAYERLEKLLPNVATFNSDSPEVPYVQGEVAIGMIWNGSAYLAQ
KENQSLQFVYPKEGAIFWMDNYAIPTTAKNVEGAHKFIDFLLRPENAKIVIERMGFSMPNNGAKTLLSAEVANDPKLFPP
AEEVEKGIMQGDVGEAVDIYEKYWSKLKTN
>Mature_350_residues
MKKFFAHSLKNLLLSTTALFAASAFANNKLYVYNWTDYVPSDLVAQFSKETGIEVIYSTFESNEEMYAKLKLTQNTGSGY
DLVFPSSYYVNKMIKEKMLQPIDQSKLTNIHQIPKHLLNKEFDPENKYSLPYVYGLTGIEVNADEIDPKTITSWADLWKP
EFKGKVLMTSDAREVFHVALLLDGKSPNTTNEEDIKTAYERLEKLLPNVATFNSDSPEVPYVQGEVAIGMIWNGSAYLAQ
KENQSLQFVYPKEGAIFWMDNYAIPTTAKNVEGAHKFIDFLLRPENAKIVIERMGFSMPNNGAKTLLSAEVANDPKLFPP
AEEVEKGIMQGDVGEAVDIYEKYWSKLKTN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=348, Percent_Identity=57.183908045977, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1787078, Length=360, Percent_Identity=33.0555555555556, Blast_Score=184, Evalue=8e-48,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39643; Mature: 39643

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFFAHSLKNLLLSTTALFAASAFANNKLYVYNWTDYVPSDLVAQFSKETGIEVIYSTF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEHHH
ESNEEMYAKLKLTQNTGSGYDLVFPSSYYVNKMIKEKMLQPIDQSKLTNIHQIPKHLLNK
HCCCEEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
EFDPENKYSLPYVYGLTGIEVNADEIDPKTITSWADLWKPEFKGKVLMTSDAREVFHVAL
CCCCCCCCCCCEEEECEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHEEEEEE
LLDGKSPNTTNEEDIKTAYERLEKLLPNVATFNSDSPEVPYVQGEVAIGMIWNGSAYLAQ
EEECCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEECCEEEEEEECCCEEEEE
KENQSLQFVYPKEGAIFWMDNYAIPTTAKNVEGAHKFIDFLLRPENAKIVIERMGFSMPN
CCCCCEEEEECCCCEEEEECCEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCC
NGAKTLLSAEVANDPKLFPPAEEVEKGIMQGDVGEAVDIYEKYWSKLKTN
CCHHHEEEHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKFFAHSLKNLLLSTTALFAASAFANNKLYVYNWTDYVPSDLVAQFSKETGIEVIYSTF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEHHH
ESNEEMYAKLKLTQNTGSGYDLVFPSSYYVNKMIKEKMLQPIDQSKLTNIHQIPKHLLNK
HCCCEEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
EFDPENKYSLPYVYGLTGIEVNADEIDPKTITSWADLWKPEFKGKVLMTSDAREVFHVAL
CCCCCCCCCCCEEEECEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHEEEEEE
LLDGKSPNTTNEEDIKTAYERLEKLLPNVATFNSDSPEVPYVQGEVAIGMIWNGSAYLAQ
EEECCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEECCEEEEEEECCCEEEEE
KENQSLQFVYPKEGAIFWMDNYAIPTTAKNVEGAHKFIDFLLRPENAKIVIERMGFSMPN
CCCCCEEEEECCCCEEEEECCEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCC
NGAKTLLSAEVANDPKLFPPAEEVEKGIMQGDVGEAVDIYEKYWSKLKTN
CCHHHEEEHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]