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The map label for this gene is holB [H]

Identifier: 148827650

GI number: 148827650

Start: 1000031

End: 1001011

Strand: Reverse

Name: holB [H]

Synonym: CGSHiGG_05505

Alternate gene names: 148827650

Gene position: 1001011-1000031 (Counterclockwise)

Preceding gene: 148827653

Following gene: 148827649

Centisome position: 53.04

GC content: 40.16

Gene sequence:

>981_bases
ATGACCGCACTTTACCCTTGGTTAATGCCAATTTATCATCAAATTGCTCAAACTTTTGACGAAGGTTTGGGGCATCATGC
TGTGCTGATTAAAGCTGATTCTGGTTTAGGTGTAGAACGTTTATTTGATGCACTTACACAGAAAATAATGTGCGTGGCTC
AAGGCGATAAACCTTGTGGTCAATGCCATTCTTGTCATTTAATGCAAGCCCATAGTCATCCAGATTATCACGAGTTAAGC
CCGATTGACGGTAAGGATATTGGCGTTGATCAAGTACGCAATATTAATGAAATGGTTGCTCAACATGCACAACAAAACGG
TAATAAAGTGGTGTATGTGCAAGGTGCGGAACGTTTAACGGAAGCAGCTGCCAATGCGTTATTGAAAACATTGGAAGAGC
CTCGTCCAAATACTTATTTTTTACTTCAAACGGATAGTTCAGCTAGTTTATTAGCAACCATTTACAGTCGTTGCCAAGTG
TGGAATCTGTCTGTGCCTAATGAACAAATGGCTGTTGATTGGTTGAAATCAGAAAGTGCGGCTGAAAATCAGGAAATTTT
GACCGCACTTGCGATGAATCTTGGGCGACCGCTTTTAGCCTTAGAAACGTTACAACAAGGGCTGATTGAACAGCGTAAAA
ACTTCTTACGTCAATTTTGGGTGTTCTATCGCCGACGTTCGCCATTGGAATTACTTCCGTTGTTTGATAAAGAACGCTAT
GTTCAGCAAGTGGATTGGATTTTGGCTTTTCTTTCTGATTGTTTAAAGCATAAACTTGAAATTGATAGCTATCGACAAGT
TGCCGATCTTGGGCGTGGTATCGAACAATTCAGCGATGAGCAAACTGCTCTTGGTTTATTACAAGCCATTAAAATTATGC
AAAAAGTGCGGTCAGATTTGCTGACGATTAATGGCGTGAATGTTGAATTAATGCTATTAGATGGCTTGACCAGATTAGTT
ACAGAAGTGTTTAAGGGTTAA

Upstream 100 bases:

>100_bases
TCTTATTTATTAAAGGTAAACGTTTGCTTTTTGCTATTTTGTCAAGCCAGTTTGAAAATGTGTATAATTGCCCTCGTTAT
TTACAAAAATTTCAGGAAAA

Downstream 100 bases:

>100_bases
TTATCAATTTACCCAAGGTACAAATTAGGAAAATAATTATGTTTATTGTTGATTCTCACTGTCATTTAGATGCGTTGGAT
TATGAAAATTTACACAAAAA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTALYPWLMPIYHQIAQTFDEGLGHHAVLIKADSGLGVERLFDALTQKIMCVAQGDKPCGQCHSCHLMQAHSHPDYHELS
PIDGKDIGVDQVRNINEMVAQHAQQNGNKVVYVQGAERLTEAAANALLKTLEEPRPNTYFLLQTDSSASLLATIYSRCQV
WNLSVPNEQMAVDWLKSESAAENQEILTALAMNLGRPLLALETLQQGLIEQRKNFLRQFWVFYRRRSPLELLPLFDKERY
VQQVDWILAFLSDCLKHKLEIDSYRQVADLGRGIEQFSDEQTALGLLQAIKIMQKVRSDLLTINGVNVELMLLDGLTRLV
TEVFKG

Sequences:

>Translated_326_residues
MTALYPWLMPIYHQIAQTFDEGLGHHAVLIKADSGLGVERLFDALTQKIMCVAQGDKPCGQCHSCHLMQAHSHPDYHELS
PIDGKDIGVDQVRNINEMVAQHAQQNGNKVVYVQGAERLTEAAANALLKTLEEPRPNTYFLLQTDSSASLLATIYSRCQV
WNLSVPNEQMAVDWLKSESAAENQEILTALAMNLGRPLLALETLQQGLIEQRKNFLRQFWVFYRRRSPLELLPLFDKERY
VQQVDWILAFLSDCLKHKLEIDSYRQVADLGRGIEQFSDEQTALGLLQAIKIMQKVRSDLLTINGVNVELMLLDGLTRLV
TEVFKG
>Mature_325_residues
TALYPWLMPIYHQIAQTFDEGLGHHAVLIKADSGLGVERLFDALTQKIMCVAQGDKPCGQCHSCHLMQAHSHPDYHELSP
IDGKDIGVDQVRNINEMVAQHAQQNGNKVVYVQGAERLTEAAANALLKTLEEPRPNTYFLLQTDSSASLLATIYSRCQVW
NLSVPNEQMAVDWLKSESAAENQEILTALAMNLGRPLLALETLQQGLIEQRKNFLRQFWVFYRRRSPLELLPLFDKERYV
QQVDWILAFLSDCLKHKLEIDSYRQVADLGRGIEQFSDEQTALGLLQAIKIMQKVRSDLLTINGVNVELMLLDGLTRLVT
EVFKG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=317, Percent_Identity=36.5930599369085, Blast_Score=172, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36915; Mature: 36784

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALYPWLMPIYHQIAQTFDEGLGHHAVLIKADSGLGVERLFDALTQKIMCVAQGDKPCG
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
QCHSCHLMQAHSHPDYHELSPIDGKDIGVDQVRNINEMVAQHAQQNGNKVVYVQGAERLT
CCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHH
EAAANALLKTLEEPRPNTYFLLQTDSSASLLATIYSRCQVWNLSVPNEQMAVDWLKSESA
HHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCC
AENQEILTALAMNLGRPLLALETLQQGLIEQRKNFLRQFWVFYRRRSPLELLPLFDKERY
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHH
VQQVDWILAFLSDCLKHKLEIDSYRQVADLGRGIEQFSDEQTALGLLQAIKIMQKVRSDL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LTINGVNVELMLLDGLTRLVTEVFKG
EEECCCCEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TALYPWLMPIYHQIAQTFDEGLGHHAVLIKADSGLGVERLFDALTQKIMCVAQGDKPCG
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
QCHSCHLMQAHSHPDYHELSPIDGKDIGVDQVRNINEMVAQHAQQNGNKVVYVQGAERLT
CCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHH
EAAANALLKTLEEPRPNTYFLLQTDSSASLLATIYSRCQVWNLSVPNEQMAVDWLKSESA
HHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCC
AENQEILTALAMNLGRPLLALETLQQGLIEQRKNFLRQFWVFYRRRSPLELLPLFDKERY
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHH
VQQVDWILAFLSDCLKHKLEIDSYRQVADLGRGIEQFSDEQTALGLLQAIKIMQKVRSDL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LTINGVNVELMLLDGLTRLVTEVFKG
EEECCCCEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]