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The map label for this gene is aspS [H]

Identifier: 148827469

GI number: 148827469

Start: 826549

End: 828315

Strand: Reverse

Name: aspS [H]

Synonym: CGSHiGG_04400

Alternate gene names: 148827469

Gene position: 828315-826549 (Counterclockwise)

Preceding gene: 148827476

Following gene: 148827468

Centisome position: 43.89

GC content: 41.65

Gene sequence:

>1767_bases
ATGATGCGTACACATTATTGCGGAGCATTAAACCGTAACAATATCGGACAAGACGTAACATTAAGCGGTTGGGTTCACCG
TCGTCGTGATTTAGGTGGTTTGATTTTTATTGATATGCGCGATCGTGATGGCATTGTGCAAGTTTATTTTGATCCTAAAT
ATCAAGATGCATTGACAGCGGCTGCGAGTTTACGTAACGAATTCTGTATCCAAATTAAAGGTGAAGTGATTGCGCGTCCT
GAAAACCAAATCAATAAAAATATGGCAACAGGCGAAGTGGAAGTGTTAGCGAAAGAATTACGCATCTACAATGCTTCTGA
TGTTTTACCATTAGACTTCAACCAAAATAACACCGAAGAACAACGTTTAAAATACCGTTATTTAGATTTACGTCGTCCTG
AAATGGCGCAGCGTTTGAAAACCCGTGCAAAAATCACCAGCTTTGTGCGTCGTTTCATGGATGATAATGGTTTCCTTGAT
ATTGAAACCCCAATGCTTACCAAAGCAACGCCAGAAGGCGCGCGTGACTATTTAGTGCCAAGCCGTGTGCATAAAGGCAA
ATTCTATGCATTGCCGCAATCACCACAGCTTTTCAAACAGCTTTTAATGATGTCTGGTTTTGACCGCTATTATCAAATCG
TAAAATGTTTCCGTGATGAAGATTTGCGAGCAGACCGTCAGCCTGAGTTTACTCAAATCGATGTGGAAACTTCTTTCCTA
ACTGCGCCAGAAGTCCGCGAAATTATGGAACGTATGGTGCACGGCTTATGGCTTGATACCATCGGTGTGGATTTAGGTAA
GTTCCCAGTGATGACTTGGCAAGAAGCAATGTGTCGTTTCGGTTCTGATAAGCCAGATTTGCGTAACCCATTAGAAATGG
TAGATGTAGCAGATATTGTCAAAGATGTTGAATTTAAAGTATTTAATGAGCCAGCAAACAATCCAAATGGCCGTGTTGCA
GTCATTCGAGTACCAAATGGTGCAGAAATTACTCGTAAACAAATTGATGAATATACACAATTTGTTGGTATTTATGGTGC
AAAAGGCTTGGCTTGGGCAAAAGTAAACGATATTAATGTTGGTCTTGAAGGCGTGCAAAGTCCGATTGCGAAATTCTTAA
ATGAAGAGGTATGGAAAGCGTTAGCAGAACGTGTGAATGCACAAACTGGCGATATTTTATTCTTCGGTGCAGACAAATGG
CAAACGACCACTGATGCAATGGGTGCGTTACGTTTGAAATTAGGTCGTGATCTTGGCTTAACTCGTTTAGACGAATGGCA
ACCGCTTTGGGTCATTGATTTCCCAATGTTTGAACGTGATGAAGAAGGTAATCTTGCAGCAATGCACCATCCATTCACTT
CACCAAAAGATTTCAGCCCAGAGCAATTAGAGGCTGATCCAACAAGTGCGGTAGCCAATGCTTACGATATGGTCATCAAC
GGCTACGAAGTGGGTGGTGGTTCTGTGCGTATTTTTGATCCGAAAATGCAACAAACTGTGTTCCGTATTCTTGGTATTGA
CGAAGAACAACAACGAGAGAAATTCGGTTTCTTATTAGATGCGTTAAAATTTGGTACTCCACCGCATGCTGGTTTAGCAT
TTGGTTTAGACCGTTTAACCATGCTTTTAACAGGCACTGAAAATATCCGTGATGTGATTGCTTTTCCTAAAACTACGGCA
GCAGCGTGCTTAATGACGGAAGCACCGAGTTTTGCGAATCCGCAAGCATTGGAAGAGTTGGCAATTTCTGTCGTTAAAGC
AGAATAA

Upstream 100 bases:

>100_bases
TTGATTTGTCAGCATAAATAAACTTTTTATCTCTCTTGGTTTTCCGCTATAATTGCGATAGATTTTTGAGCATAATTAAA
AGAGTACAAAGGATTTTGAA

Downstream 100 bases:

>100_bases
GTTTGAAATATTAGGAATGAAGTGCGGTCAGATTTGACCGCATTTTTAATGATGCAATACAAAAATAATCAGTCTGTTCT
CGTTGTAATTTACACTAAAG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVYFDPKYQDALTAAASLRNEFCIQIKGEVIARP
ENQINKNMATGEVEVLAKELRIYNASDVLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLD
IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMCRFGSDKPDLRNPLEMVDVADIVKDVEFKVFNEPANNPNGRVA
VIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINVGLEGVQSPIAKFLNEEVWKALAERVNAQTGDILFFGADKW
QTTTDAMGALRLKLGRDLGLTRLDEWQPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLTMLLTGTENIRDVIAFPKTTA
AACLMTEAPSFANPQALEELAISVVKAE

Sequences:

>Translated_588_residues
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVYFDPKYQDALTAAASLRNEFCIQIKGEVIARP
ENQINKNMATGEVEVLAKELRIYNASDVLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLD
IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMCRFGSDKPDLRNPLEMVDVADIVKDVEFKVFNEPANNPNGRVA
VIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINVGLEGVQSPIAKFLNEEVWKALAERVNAQTGDILFFGADKW
QTTTDAMGALRLKLGRDLGLTRLDEWQPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLTMLLTGTENIRDVIAFPKTTA
AACLMTEAPSFANPQALEELAISVVKAE
>Mature_588_residues
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVYFDPKYQDALTAAASLRNEFCIQIKGEVIARP
ENQINKNMATGEVEVLAKELRIYNASDVLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLD
IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMCRFGSDKPDLRNPLEMVDVADIVKDVEFKVFNEPANNPNGRVA
VIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINVGLEGVQSPIAKFLNEEVWKALAERVNAQTGDILFFGADKW
QTTTDAMGALRLKLGRDLGLTRLDEWQPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLTMLLTGTENIRDVIAFPKTTA
AACLMTEAPSFANPQALEELAISVVKAE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=604, Percent_Identity=37.9139072847682, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI5031815, Length=253, Percent_Identity=29.2490118577075, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI194272210, Length=253, Percent_Identity=29.2490118577075, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI45439306, Length=253, Percent_Identity=24.901185770751, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI226491502, Length=271, Percent_Identity=23.2472324723247, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788173, Length=585, Percent_Identity=76.4102564102564, Blast_Score=929, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=289, Percent_Identity=28.3737024221453, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790571, Length=289, Percent_Identity=27.681660899654, Blast_Score=93, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=600, Percent_Identity=33.8333333333333, Blast_Score=314, Evalue=8e-86,
Organism=Caenorhabditis elegans, GI71994340, Length=248, Percent_Identity=24.1935483870968, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=248, Percent_Identity=24.1935483870968, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=263, Percent_Identity=23.574144486692, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=298, Percent_Identity=27.5167785234899, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=313, Percent_Identity=37.3801916932907, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6320242, Length=259, Percent_Identity=24.7104247104247, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=250, Percent_Identity=26, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323011, Length=245, Percent_Identity=26.530612244898, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24584738, Length=610, Percent_Identity=36.8852459016393, Blast_Score=342, Evalue=6e-94,
Organism=Drosophila melanogaster, GI24640849, Length=264, Percent_Identity=29.5454545454545, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640851, Length=332, Percent_Identity=27.710843373494, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17136276, Length=241, Percent_Identity=26.1410788381743, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66747; Mature: 66747

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVYFDPKYQDALTA
CCCCCCCCCCCCCCCCCCCEEHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHH
AASLRNEFCIQIKGEVIARPENQINKNMATGEVEVLAKELRIYNASDVLPLDFNQNNTEE
HHHHCCCEEEEECCCEEECCHHHHHCCCCCCHHHHHHHHHHEECCCCEEEECCCCCCCHH
QRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLDIETPMLTKATPEGARDYLVP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCCCCC
SRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
CCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHCC
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMCRFGSDKPDLRNPLEMVDVADIV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
KDVEFKVFNEPANNPNGRVAVIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINV
HHCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
GLEGVQSPIAKFLNEEVWKALAERVNAQTGDILFFGADKWQTTTDAMGALRLKLGRDLGL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCC
TRLDEWQPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHEEE
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLT
EEEECCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MLLTGTENIRDVIAFPKTTAAACLMTEAPSFANPQALEELAISVVKAE
EEEECCHHHHHHHCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVYFDPKYQDALTA
CCCCCCCCCCCCCCCCCCCEEHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHH
AASLRNEFCIQIKGEVIARPENQINKNMATGEVEVLAKELRIYNASDVLPLDFNQNNTEE
HHHHCCCEEEEECCCEEECCHHHHHCCCCCCHHHHHHHHHHEECCCCEEEECCCCCCCHH
QRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLDIETPMLTKATPEGARDYLVP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCCCCC
SRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
CCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHCC
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMCRFGSDKPDLRNPLEMVDVADIV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
KDVEFKVFNEPANNPNGRVAVIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINV
HHCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
GLEGVQSPIAKFLNEEVWKALAERVNAQTGDILFFGADKWQTTTDAMGALRLKLGRDLGL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCC
TRLDEWQPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHEEE
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLT
EEEECCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MLLTGTENIRDVIAFPKTTAAACLMTEAPSFANPQALEELAISVVKAE
EEEECCHHHHHHHCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA