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The map label for this gene is menF [H]

Identifier: 148827437

GI number: 148827437

Start: 796578

End: 797735

Strand: Reverse

Name: menF [H]

Synonym: CGSHiGG_04215

Alternate gene names: 148827437

Gene position: 797735-796578 (Counterclockwise)

Preceding gene: 148827441

Following gene: 148827436

Centisome position: 42.27

GC content: 38.86

Gene sequence:

>1158_bases
TTGGCGTGGTTGAAAGGTCAATCTGCGTATCCTCAATTTTATTTACATTTTCGTGATGAAGAAAAAGCACTGGCTGCGCT
TGGGGCAGTGCAGTCATTTTCACAGTTAAATTTAGCGCAAGAGTTTATTGAAGAAAGTGGTTTTCCACTGGTAGGCGGTT
TGCAATTTCAAGGCACTGCACAATTTGTATTACCTAAAATGCTTGTTGAACAGGATAATAAAGGCACGTTGGTATCCTTT
TTTGTGAAAAATGAGCAAAGTGCGAATGATACTTTAGCCCACCTCAAAACTTTTGAAAATCTCACCGCACTTTCTGCGTT
ACCAAAACAAATTCCTTTGCATACAGAGCCTCGAGCTAACGAAAGAACTTGGTGTGATTGGGTAAATCAGGCTTTGGTAG
AAATTAAAAGTGGAGAGCTGACAAAAATTGTGTTAGCCAATGAAACCACTTTTCATTTAAAGCAAGCGATTAATGCGTAC
GATTTTTTAGCAGAAAGCGAAAAAAAAAATCAAGGTTGTTATCATTTTTTATGGGCTGAAAATTCTTATTCGGTTTTTGT
TGGTTCTACGCCAGAACGCCTATTTGCTCGTGAGTATAATTTGTTGCTAACCGAAGCTTTGGCGGGGACAGCATCAGTTT
CTGAAAGTGAGGAAGAAACGCAATCTCAAGCCAATTGGTTATTAAATGATGAGAAAAATTTAAAAGAAAATTGGTTGGTT
GTAGAAGATATTTCGCAGAATTTACGTAAGCAAGTAGAAAGTTTTGATGTAAGCAATGTGGAATTGAAACCGTTACGTAA
AGTACAACATTTGATTCGTAAAATTCGTGCAAATTTGACCGCACATTATGCAGATGTAAATATATTAAAAGCGATTCATC
CTACAGCTGCAGTATCTGGTTTGCCACAGCAACAAGCCAAAATGATTTTGTCAGAAATCGAAACCTTTGATCGAGGCTGG
TATGCGGGAACATTGGGCGTGATGAGCGATGTATGTTCAGAGTTTTGTGTGGCGATTCGTTCTGCTTTTATTGAAGGTCA
TCGTATTCGAGTATTTGCTGGCGCGGGCATTGTGGCAGGCTCGCAGCCATTGGAAGAATGGAAAGAAATTGAACGTAAAG
CAGCAGGGTTAATTTCCTTGTTTGCAGAAGAAAAATGA

Upstream 100 bases:

>100_bases
GCAGGCAATAGGCGAGTTAAAATCGCAAATTCACGCATATTTACAGCAATCAACGAATGAACTTGTCCGTTTTCAGGTAA
AACTGGACAAGGTCGATTTG

Downstream 100 bases:

>100_bases
TAACGGAGAAAAAAAATGTCGGTAAGCGTGTTTAATCGTTGTTGGTCGAAAGTGATTTTAGAAACCTTGGTGCGCCAAGG
CGTTTCTCACGTTTGTATCG

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MAWLKGQSAYPQFYLHFRDEEKALAALGAVQSFSQLNLAQEFIEESGFPLVGGLQFQGTAQFVLPKMLVEQDNKGTLVSF
FVKNEQSANDTLAHLKTFENLTALSALPKQIPLHTEPRANERTWCDWVNQALVEIKSGELTKIVLANETTFHLKQAINAY
DFLAESEKKNQGCYHFLWAENSYSVFVGSTPERLFAREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLV
VEDISQNLRKQVESFDVSNVELKPLRKVQHLIRKIRANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIETFDRGW
YAGTLGVMSDVCSEFCVAIRSAFIEGHRIRVFAGAGIVAGSQPLEEWKEIERKAAGLISLFAEEK

Sequences:

>Translated_385_residues
MAWLKGQSAYPQFYLHFRDEEKALAALGAVQSFSQLNLAQEFIEESGFPLVGGLQFQGTAQFVLPKMLVEQDNKGTLVSF
FVKNEQSANDTLAHLKTFENLTALSALPKQIPLHTEPRANERTWCDWVNQALVEIKSGELTKIVLANETTFHLKQAINAY
DFLAESEKKNQGCYHFLWAENSYSVFVGSTPERLFAREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLV
VEDISQNLRKQVESFDVSNVELKPLRKVQHLIRKIRANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIETFDRGW
YAGTLGVMSDVCSEFCVAIRSAFIEGHRIRVFAGAGIVAGSQPLEEWKEIERKAAGLISLFAEEK
>Mature_384_residues
AWLKGQSAYPQFYLHFRDEEKALAALGAVQSFSQLNLAQEFIEESGFPLVGGLQFQGTAQFVLPKMLVEQDNKGTLVSFF
VKNEQSANDTLAHLKTFENLTALSALPKQIPLHTEPRANERTWCDWVNQALVEIKSGELTKIVLANETTFHLKQAINAYD
FLAESEKKNQGCYHFLWAENSYSVFVGSTPERLFAREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLVV
EDISQNLRKQVESFDVSNVELKPLRKVQHLIRKIRANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIETFDRGWY
AGTLGVMSDVCSEFCVAIRSAFIEGHRIRVFAGAGIVAGSQPLEEWKEIERKAAGLISLFAEEK

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=397, Percent_Identity=38.2871536523929, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1786809, Length=262, Percent_Identity=28.6259541984733, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787518, Length=340, Percent_Identity=23.2352941176471, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788114, Length=245, Percent_Identity=25.7142857142857, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=249, Percent_Identity=25.7028112449799, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 43242; Mature: 43111

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWLKGQSAYPQFYLHFRDEEKALAALGAVQSFSQLNLAQEFIEESGFPLVGGLQFQGTA
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEECCCH
QFVLPKMLVEQDNKGTLVSFFVKNEQSANDTLAHLKTFENLTALSALPKQIPLHTEPRAN
HHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ERTWCDWVNQALVEIKSGELTKIVLANETTFHLKQAINAYDFLAESEKKNQGCYHFLWAE
CCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
NSYSVFVGSTPERLFAREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLV
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHCCEE
VEDISQNLRKQVESFDVSNVELKPLRKVQHLIRKIRANLTAHYADVNILKAIHPTAAVSG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHCC
LPQQQAKMILSEIETFDRGWYAGTLGVMSDVCSEFCVAIRSAFIEGHRIRVFAGAGIVAG
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECC
SQPLEEWKEIERKAAGLISLFAEEK
CCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AWLKGQSAYPQFYLHFRDEEKALAALGAVQSFSQLNLAQEFIEESGFPLVGGLQFQGTA
CCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEECCCH
QFVLPKMLVEQDNKGTLVSFFVKNEQSANDTLAHLKTFENLTALSALPKQIPLHTEPRAN
HHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ERTWCDWVNQALVEIKSGELTKIVLANETTFHLKQAINAYDFLAESEKKNQGCYHFLWAE
CCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
NSYSVFVGSTPERLFAREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLV
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHCCEE
VEDISQNLRKQVESFDVSNVELKPLRKVQHLIRKIRANLTAHYADVNILKAIHPTAAVSG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHCC
LPQQQAKMILSEIETFDRGWYAGTLGVMSDVCSEFCVAIRSAFIEGHRIRVFAGAGIVAG
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECC
SQPLEEWKEIERKAAGLISLFAEEK
CCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]