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The map label for this gene is menD [H]

Identifier: 148827436

GI number: 148827436

Start: 794856

End: 796562

Strand: Reverse

Name: menD [H]

Synonym: CGSHiGG_04210

Alternate gene names: 148827436

Gene position: 796562-794856 (Counterclockwise)

Preceding gene: 148827437

Following gene: 148827435

Centisome position: 42.21

GC content: 40.95

Gene sequence:

>1707_bases
ATGTCGGTAAGCGTGTTTAATCGTTGTTGGTCGAAAGTGATTTTAGAAACCTTGGTGCGCCAAGGCGTTTCTCACGTTTG
TATCGCGCCAGGTTCGCGTTCGACACCTTTAACTCTAGAAGCCGTACGTTTGCAAAATGCAGGTTCAGTCACTTGTCATA
CGCATTTTGATGAACGCGGTTTAGGTTTTTTTGCTTTGGGTATTGCTAAGGCAACTCAATCGCCTGTTGCAATTATTGTT
ACATCAGGTACTGCAACAGCAAACCTTTATCCCGCCATTATTGAAGCACGCCAAACTGGTGTGAATTTATTTGTTTTAAC
TGCTGATCGTCCACCAGAACTTTGGGAGTGCGGTGCAAATCAAGCTATTTTGCAACAAAATATGTTTGGTCAGTATCCAG
TTGCAAATGTTAATTTACCTAAACCGAATGCTGATTATTCTGCGCAGTGGCTAATTTCTCTACTTGAACAGGCAGCTTTC
CAACAAAAACAACAAGGTGGCGTTGTTCATATTAATGTCCCATTTGCCGAGCCACTTTATGATGCAACTGATGAGGAAGT
AAATTCCCATAGTTGGCTACAGCCGTTACAACGCTGGTTAATTCAAAAAAAGTCTTGGATAAATGTAGAAGTCCAACAAA
ACGAAGTATTAATGCATGAAAATTGGGATCATTGGCGTACCAAACGTGGTGTCGTTGTGGTTGGTCAATTACCTGCAGAA
CAAGCGATGGGCATTAATTCTTGGGCAAGTGCTATGGGCTGGGTGTTACTGACGGATATTCAATCTGGTGTTGTTCCAAC
GACGCCTTATGAAGATATTTGGCTAGCAAACCAAACTGTTCGTGAAAAACTTCTACAAGCTGATATTGTGATTCAATTTG
GCGCGCGTTTTATTAGTAAACGCATTAATCAATTCTTGCAAGCGTTTAAAGGGGAATTTTGGCTAGTTGAACAAAGCGGT
AAAGCATTAGATCCTTACCATCATTCATTGACAAGATTTAATGCTAAAGTACATCATTGGTTGCGTGCACATCCACCTTT
ACGCCAAAAGCCTTGGTTGCTTGAGCCGTTAGCTTTATCTAAGTTCTGTGCGACCTTTATTGAACAGCAAGTAGGCGGAA
ATTTAACGGAAGCCTCGCTCGCATTACGTTTACCAACACTTTTACCTTATAACGGTGTTTTATTTTTAGGTAATAGTTTA
CTTGTTCGTCTAGTTGATGCGCTAACGCAATTACCAGAAAGTTATCCCGTTTATACCAATCGTGGTGCGAGTGGAATTGA
TGGTTTGTTGGCTACTGCTGCTGGAATAGGTATTGGTTCAAATAAACCTGTTGTTGCGGTGATTGGCGATACGTCCACTT
TATATGATTTGAATTCTTTCGCATTATTCAAAAACGTTACGCAACCAACGCTAATCTTTGTGATCAATAATAATGGCGGC
GCGATTTTTGATATGTTACCTGTGGATGAACAAGTCAAAGATCAGTTCTATCGATTACCGCATAATGGCGATTTTTCTCA
AATCGCGGCAATGTTCGATCTCAAATACGCTCATCCTTATACTTGGGCGGATCTCAATTCTGTTGTGAAACAGGCTTATA
GTCGCCGCAAGGCAACGTTAATTGAAATTAAAACGAATCCGAGTGATGGTAGCAGTTTGTATAAACGCTTAATCGAGCAA
ATTAGTCACGCCGTGATTGGTGCTTAA

Upstream 100 bases:

>100_bases
GGCAGGCTCGCAGCCATTGGAAGAATGGAAAGAAATTGAACGTAAAGCAGCAGGGTTAATTTCCTTGTTTGCAGAAGAAA
AATGATAACGGAGAAAAAAA

Downstream 100 bases:

>100_bases
GTATTCTGTAGGCGGGCTTTAGTCCGCCATATTTTATTATTTTCTATCAATAATCCCTTCAATGATAAACATCATTTTTC
TTCACGGACTTCTTGGTACA

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MSVSVFNRCWSKVILETLVRQGVSHVCIAPGSRSTPLTLEAVRLQNAGSVTCHTHFDERGLGFFALGIAKATQSPVAIIV
TSGTATANLYPAIIEARQTGVNLFVLTADRPPELWECGANQAILQQNMFGQYPVANVNLPKPNADYSAQWLISLLEQAAF
QQKQQGGVVHINVPFAEPLYDATDEEVNSHSWLQPLQRWLIQKKSWINVEVQQNEVLMHENWDHWRTKRGVVVVGQLPAE
QAMGINSWASAMGWVLLTDIQSGVVPTTPYEDIWLANQTVREKLLQADIVIQFGARFISKRINQFLQAFKGEFWLVEQSG
KALDPYHHSLTRFNAKVHHWLRAHPPLRQKPWLLEPLALSKFCATFIEQQVGGNLTEASLALRLPTLLPYNGVLFLGNSL
LVRLVDALTQLPESYPVYTNRGASGIDGLLATAAGIGIGSNKPVVAVIGDTSTLYDLNSFALFKNVTQPTLIFVINNNGG
AIFDMLPVDEQVKDQFYRLPHNGDFSQIAAMFDLKYAHPYTWADLNSVVKQAYSRRKATLIEIKTNPSDGSSLYKRLIEQ
ISHAVIGA

Sequences:

>Translated_568_residues
MSVSVFNRCWSKVILETLVRQGVSHVCIAPGSRSTPLTLEAVRLQNAGSVTCHTHFDERGLGFFALGIAKATQSPVAIIV
TSGTATANLYPAIIEARQTGVNLFVLTADRPPELWECGANQAILQQNMFGQYPVANVNLPKPNADYSAQWLISLLEQAAF
QQKQQGGVVHINVPFAEPLYDATDEEVNSHSWLQPLQRWLIQKKSWINVEVQQNEVLMHENWDHWRTKRGVVVVGQLPAE
QAMGINSWASAMGWVLLTDIQSGVVPTTPYEDIWLANQTVREKLLQADIVIQFGARFISKRINQFLQAFKGEFWLVEQSG
KALDPYHHSLTRFNAKVHHWLRAHPPLRQKPWLLEPLALSKFCATFIEQQVGGNLTEASLALRLPTLLPYNGVLFLGNSL
LVRLVDALTQLPESYPVYTNRGASGIDGLLATAAGIGIGSNKPVVAVIGDTSTLYDLNSFALFKNVTQPTLIFVINNNGG
AIFDMLPVDEQVKDQFYRLPHNGDFSQIAAMFDLKYAHPYTWADLNSVVKQAYSRRKATLIEIKTNPSDGSSLYKRLIEQ
ISHAVIGA
>Mature_567_residues
SVSVFNRCWSKVILETLVRQGVSHVCIAPGSRSTPLTLEAVRLQNAGSVTCHTHFDERGLGFFALGIAKATQSPVAIIVT
SGTATANLYPAIIEARQTGVNLFVLTADRPPELWECGANQAILQQNMFGQYPVANVNLPKPNADYSAQWLISLLEQAAFQ
QKQQGGVVHINVPFAEPLYDATDEEVNSHSWLQPLQRWLIQKKSWINVEVQQNEVLMHENWDHWRTKRGVVVVGQLPAEQ
AMGINSWASAMGWVLLTDIQSGVVPTTPYEDIWLANQTVREKLLQADIVIQFGARFISKRINQFLQAFKGEFWLVEQSGK
ALDPYHHSLTRFNAKVHHWLRAHPPLRQKPWLLEPLALSKFCATFIEQQVGGNLTEASLALRLPTLLPYNGVLFLGNSLL
VRLVDALTQLPESYPVYTNRGASGIDGLLATAAGIGIGSNKPVVAVIGDTSTLYDLNSFALFKNVTQPTLIFVINNNGGA
IFDMLPVDEQVKDQFYRLPHNGDFSQIAAMFDLKYAHPYTWADLNSVVKQAYSRRKATLIEIKTNPSDGSSLYKRLIEQI
SHAVIGA

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=566, Percent_Identity=47.5265017667844, Blast_Score=516, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 63114; Mature: 62983

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSVFNRCWSKVILETLVRQGVSHVCIAPGSRSTPLTLEAVRLQNAGSVTCHTHFDERG
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEEECCCCCEEEEECCCCCC
LGFFALGIAKATQSPVAIIVTSGTATANLYPAIIEARQTGVNLFVLTADRPPELWECGAN
CCEEEEEHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCC
QAILQQNMFGQYPVANVNLPKPNADYSAQWLISLLEQAAFQQKQQGGVVHINVPFAEPLY
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
DATDEEVNSHSWLQPLQRWLIQKKSWINVEVQQNEVLMHENWDHWRTKRGVVVVGQLPAE
CCCCHHCCCHHHHHHHHHHHHCCCCEEEEEEECCEEEECCCCHHHHCCCCEEEEECCCHH
QAMGINSWASAMGWVLLTDIQSGVVPTTPYEDIWLANQTVREKLLQADIVIQFGARFISK
HHCCCHHHHHHHCCEEEEEHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
RINQFLQAFKGEFWLVEQSGKALDPYHHSLTRFNAKVHHWLRAHPPLRQKPWLLEPLALS
HHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
KFCATFIEQQVGGNLTEASLALRLPTLLPYNGVLFLGNSLLVRLVDALTQLPESYPVYTN
HHHHHHHHHHHCCCCCHHHHEEECCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCEEC
RGASGIDGLLATAAGIGIGSNKPVVAVIGDTSTLYDLNSFALFKNVTQPTLIFVINNNGG
CCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECHHHHHHHCCCCCEEEEEEECCCC
AIFDMLPVDEQVKDQFYRLPHNGDFSQIAAMFDLKYAHPYTWADLNSVVKQAYSRRKATL
EEEEECCCCHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEE
IEIKTNPSDGSSLYKRLIEQISHAVIGA
EEEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVSVFNRCWSKVILETLVRQGVSHVCIAPGSRSTPLTLEAVRLQNAGSVTCHTHFDERG
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEEECCCCCEEEEECCCCCC
LGFFALGIAKATQSPVAIIVTSGTATANLYPAIIEARQTGVNLFVLTADRPPELWECGAN
CCEEEEEHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCC
QAILQQNMFGQYPVANVNLPKPNADYSAQWLISLLEQAAFQQKQQGGVVHINVPFAEPLY
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
DATDEEVNSHSWLQPLQRWLIQKKSWINVEVQQNEVLMHENWDHWRTKRGVVVVGQLPAE
CCCCHHCCCHHHHHHHHHHHHCCCCEEEEEEECCEEEECCCCHHHHCCCCEEEEECCCHH
QAMGINSWASAMGWVLLTDIQSGVVPTTPYEDIWLANQTVREKLLQADIVIQFGARFISK
HHCCCHHHHHHHCCEEEEEHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
RINQFLQAFKGEFWLVEQSGKALDPYHHSLTRFNAKVHHWLRAHPPLRQKPWLLEPLALS
HHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
KFCATFIEQQVGGNLTEASLALRLPTLLPYNGVLFLGNSLLVRLVDALTQLPESYPVYTN
HHHHHHHHHHHCCCCCHHHHEEECCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCEEC
RGASGIDGLLATAAGIGIGSNKPVVAVIGDTSTLYDLNSFALFKNVTQPTLIFVINNNGG
CCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECHHHHHHHCCCCCEEEEEEECCCC
AIFDMLPVDEQVKDQFYRLPHNGDFSQIAAMFDLKYAHPYTWADLNSVVKQAYSRRKATL
EEEEECCCCHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEE
IEIKTNPSDGSSLYKRLIEQISHAVIGA
EEEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA