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The map label for this gene is brnQ [H]

Identifier: 148827367

GI number: 148827367

Start: 712150

End: 713493

Strand: Direct

Name: brnQ [H]

Synonym: CGSHiGG_03760

Alternate gene names: 148827367

Gene position: 712150-713493 (Clockwise)

Preceding gene: 148827365

Following gene: 148827370

Centisome position: 37.74

GC content: 37.28

Gene sequence:

>1344_bases
ATGCAGGCCTTTTTATTTTTAGAGTCAAAAATTATGTTTTCACGTAAAGATATTATCGTTTTAGGAATGATGATCTTCGC
CTTATTTTTAGGTGCAGGTAATATCATTTTTCCGCCTATGGAAGGATTTTCCTCAGGACAACATTGGGCGAGTGCTTCCT
TAGGCTTTGTTTTAACTGGCGTTTTAATGCCATTTATTACGCTTGTTGTGGTAGCGATTTTAGGTCGTGGCGAAGAACTT
ACTAAAGATTTACCAAAATGGGCTGGCACAGGTTTCCTCATTATACTTTATTTAACCATTGGCTCTACCTTTGCAATGCC
CCGCATCACTAATGTTGCTTATGAAATGGCGTGGTTGCCTTTAGGCTTAACAGAAAATAATGCGAATGTTCGTTTTGTAT
TCTCACTAATTTTTAACTTAATCGCGATGGGCTTTATGATTAGCCCAAATACGATCATTTCAAGCGTGGGTAAATTTATG
ACACCAGCTTTATTAGTGTTACTTATTGCTGTAGCAATTACCGTATTTATTTCGCCTTTATCTGAAATTCAAGCGCCAAG
TAATGCTTATGAAAATAGCCATTCTTTATTAATCGGCTTAACCAGTGGTTATCAAACCATGGATGTACTCGCAGCCATCG
CTTTCGGTGGAATTGTCGCCCGAGCATTATCAGCGAAAAATGTGACAAAAACCAAAGATATTGTGAAATACACCATTTCA
GCAGGATTTGTTTCTGTCATTTTACTCGCCAGTTTATATTTTTCGCTTTTCTATCTAGGTGCAACCAGTGCTGCAGTTGC
AGAAGGGGCAACGAATGGTGGACAAATTTTCTCTCGTTATGTAAATGTCTTATTCGGTTCAGCTGGGACTTGGATTATGG
CAGGTATTATTGTGTTAGCGAGCTTAACAACACTTGTTGGTGTAACTAGCGCATCTGCTGATTATTTTTCAAAATTCTCT
GTGCGTTTTTCTTATCCATTCTGGGCCGCACTTTTTACCGCAATGACAATCACTGTTTCACAATATGGTTTAACGGATCT
ACTTCGCATAACGATCCCAGCCTTGTTATTAATTTACCCAGTAGCTATTGTGCTTGTTTTATTGCAATTCTTACGAAAAA
AACTTCCATCAATAAAATTTACATACAATAGTACATTGCTCGTTACAGTTTGTTTTAGTTTATGTGACAGCTTAAATAAC
GTTAAAATGTTACCAGAAAGCATCAATTCATTATTGAAACACTTCCCTCTTTCATCTGAAGGAATGGCTTGGTTGGTCCC
GACTTTAGTAATGTTAGTTGCAAGCATTTTTATTGGAAAAGCATTGCGTAAAACCCATTCTTAA

Upstream 100 bases:

>100_bases
GTCGGGATTTTTAGCGAATTGTCGATAAAATGCCATATATCGTGACGAAATTCACATTTTTAGTTTTACAGAATAGGGAT
TCTCTACACTTTGTAGTAGA

Downstream 100 bases:

>100_bases
TCTCACAAAGTGTTTTATTTATCAGCCCTGTGCCGTAAAACTCCGTCCTTCAGGGCGGAGATATAAGGCACAGGGCTGAT
AAATTTCTAACCGCACTTTT

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MQAFLFLESKIMFSRKDIIVLGMMIFALFLGAGNIIFPPMEGFSSGQHWASASLGFVLTGVLMPFITLVVVAILGRGEEL
TKDLPKWAGTGFLIILYLTIGSTFAMPRITNVAYEMAWLPLGLTENNANVRFVFSLIFNLIAMGFMISPNTIISSVGKFM
TPALLVLLIAVAITVFISPLSEIQAPSNAYENSHSLLIGLTSGYQTMDVLAAIAFGGIVARALSAKNVTKTKDIVKYTIS
AGFVSVILLASLYFSLFYLGATSAAVAEGATNGGQIFSRYVNVLFGSAGTWIMAGIIVLASLTTLVGVTSASADYFSKFS
VRFSYPFWAALFTAMTITVSQYGLTDLLRITIPALLLIYPVAIVLVLLQFLRKKLPSIKFTYNSTLLVTVCFSLCDSLNN
VKMLPESINSLLKHFPLSSEGMAWLVPTLVMLVASIFIGKALRKTHS

Sequences:

>Translated_447_residues
MQAFLFLESKIMFSRKDIIVLGMMIFALFLGAGNIIFPPMEGFSSGQHWASASLGFVLTGVLMPFITLVVVAILGRGEEL
TKDLPKWAGTGFLIILYLTIGSTFAMPRITNVAYEMAWLPLGLTENNANVRFVFSLIFNLIAMGFMISPNTIISSVGKFM
TPALLVLLIAVAITVFISPLSEIQAPSNAYENSHSLLIGLTSGYQTMDVLAAIAFGGIVARALSAKNVTKTKDIVKYTIS
AGFVSVILLASLYFSLFYLGATSAAVAEGATNGGQIFSRYVNVLFGSAGTWIMAGIIVLASLTTLVGVTSASADYFSKFS
VRFSYPFWAALFTAMTITVSQYGLTDLLRITIPALLLIYPVAIVLVLLQFLRKKLPSIKFTYNSTLLVTVCFSLCDSLNN
VKMLPESINSLLKHFPLSSEGMAWLVPTLVMLVASIFIGKALRKTHS
>Mature_447_residues
MQAFLFLESKIMFSRKDIIVLGMMIFALFLGAGNIIFPPMEGFSSGQHWASASLGFVLTGVLMPFITLVVVAILGRGEEL
TKDLPKWAGTGFLIILYLTIGSTFAMPRITNVAYEMAWLPLGLTENNANVRFVFSLIFNLIAMGFMISPNTIISSVGKFM
TPALLVLLIAVAITVFISPLSEIQAPSNAYENSHSLLIGLTSGYQTMDVLAAIAFGGIVARALSAKNVTKTKDIVKYTIS
AGFVSVILLASLYFSLFYLGATSAAVAEGATNGGQIFSRYVNVLFGSAGTWIMAGIIVLASLTTLVGVTSASADYFSKFS
VRFSYPFWAALFTAMTITVSQYGLTDLLRITIPALLLIYPVAIVLVLLQFLRKKLPSIKFTYNSTLLVTVCFSLCDSLNN
VKMLPESINSLLKHFPLSSEGMAWLVPTLVMLVASIFIGKALRKTHS

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=430, Percent_Identity=34.1860465116279, Blast_Score=256, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48381; Mature: 48381

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAFLFLESKIMFSRKDIIVLGMMIFALFLGAGNIIFPPMEGFSSGQHWASASLGFVLTG
CCEEEEEHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHH
VLMPFITLVVVAILGRGEELTKDLPKWAGTGFLIILYLTIGSTFAMPRITNVAYEMAWLP
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHCCCHHHHHHHHHHHEE
LGLTENNANVRFVFSLIFNLIAMGFMISPNTIISSVGKFMTPALLVLLIAVAITVFISPL
EEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEIQAPSNAYENSHSLLIGLTSGYQTMDVLAAIAFGGIVARALSAKNVTKTKDIVKYTIS
HHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AGFVSVILLASLYFSLFYLGATSAAVAEGATNGGQIFSRYVNVLFGSAGTWIMAGIIVLA
HHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SLTTLVGVTSASADYFSKFSVRFSYPFWAALFTAMTITVSQYGLTDLLRITIPALLLIYP
HHHHHHHHHCCCHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAIVLVLLQFLRKKLPSIKFTYNSTLLVTVCFSLCDSLNNVKMLPESINSLLKHFPLSSE
HHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
GMAWLVPTLVMLVASIFIGKALRKTHS
CCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQAFLFLESKIMFSRKDIIVLGMMIFALFLGAGNIIFPPMEGFSSGQHWASASLGFVLTG
CCEEEEEHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHH
VLMPFITLVVVAILGRGEELTKDLPKWAGTGFLIILYLTIGSTFAMPRITNVAYEMAWLP
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHCCCHHHHHHHHHHHEE
LGLTENNANVRFVFSLIFNLIAMGFMISPNTIISSVGKFMTPALLVLLIAVAITVFISPL
EEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEIQAPSNAYENSHSLLIGLTSGYQTMDVLAAIAFGGIVARALSAKNVTKTKDIVKYTIS
HHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AGFVSVILLASLYFSLFYLGATSAAVAEGATNGGQIFSRYVNVLFGSAGTWIMAGIIVLA
HHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SLTTLVGVTSASADYFSKFSVRFSYPFWAALFTAMTITVSQYGLTDLLRITIPALLLIYP
HHHHHHHHHCCCHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAIVLVLLQFLRKKLPSIKFTYNSTLLVTVCFSLCDSLNNVKMLPESINSLLKHFPLSSE
HHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
GMAWLVPTLVMLVASIFIGKALRKTHS
CCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]