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The map label for this gene is ushA [C]

Identifier: 148827344

GI number: 148827344

Start: 676976

End: 678787

Strand: Direct

Name: ushA [C]

Synonym: CGSHiGG_03645

Alternate gene names: 148827344

Gene position: 676976-678787 (Clockwise)

Preceding gene: 148827343

Following gene: 148827345

Centisome position: 35.87

GC content: 36.31

Gene sequence:

>1812_bases
ATGCTTTTATCCAAAAAATCGGCCACCTTTGCACTCAGTGCATTTGCGATGCTTTTCACTAGTGTAGCTCTTGCCAAAGA
GGCACCACAAGCTCACAAAGCTGTGGAATTAAGTATTTTGCACATCAATGATCACCATTCTTATTTAGAACCGCACGAAA
CACGGATTAATTTAAATGGTCAGCAAACCAAAGTGGATATTGGTGGTTTTTCTGCTGTCAATGCAAAACTTAACAAATTG
CGTAAAAAATACAAAAATCCATTAGTACTGCATGCAGGCGATGCCATTACTGGTACGCTTTACTTCACGCTGTTTGGTGG
TTCTGCAGATGCAGCTGTGATGAATGCAGGTAATTTCCATTATTTTACTTTAGGTAATCATGAATTTGACGCGGGTAATG
AAGGGTTATTAAAACTGCTTGAACCATTAAAAATCCCAGTGCTTTCAGCTAATGTGATTCCTGATAAAAATTCAATTTTG
TATAACAAATGGAAACCTTACGATATTTTCACTGTGGATGGAGAAAAAATTGCCATTATCGGTTTAGATACCGTGAATAA
AACAGTGAATTCCTCTTCTCCGGGTAAGGATGTGAAGTTTTATGATGAAATTGCTACTGCACAAATTATGGCAAATGCTT
TAAAACAACAAGGCATTAATAAAATTATCCTACTTTCACACGCAGGTAGTGAAAAAAATATCGAAATTGCTCAAAAAGTA
AATGATATTGATGTGATCGTTACTGGCGATTCACATTATTTATACGGAAATGATGAATTACGTAGTTTAAAACTTCCAGT
AATCTATGAATATCCACTTGAATTTAAAAATCCAAATGGAGAGCCTGTATTTGTAATGGAAGGTTGGGCTTATTCTGCTG
TTGTGGGTGATTTAGGTGTTAAATTCAGTCCTGAAGGTATTGCGTCTATTACTCGTAAAATTCCTCACGTGTTAATGAGC
TCTCATAAACTTCAAGTGAAAAATTCGGAAGGTAAATGGGCTGAATTAACTGGCGATGAACGTAAAAAAGCACTTGATAC
TTTAAAATCAATGAAAAGTATTTCACTTGATGATCATGATGCAAAAACAGACATGCTTATTTCAAAATATAAAAGTGAAA
AAGATCGTTTAGCACAAGAAATTGTTGGTGTCATCACAGGTTCTGCAATGCCGGGTGGTTCAGCAAACCGTATCCCAAAT
AAAGCAGGATCTAATCCAGAAGGTTCTATTGCAACGCGTTTTATTGCAGAAACAATGTATAACGAACTCAAAACAGTGGA
TTTAACTATTCAAAATGCTGGCGGTGTACGCGCAGATATTTTACCGGGCAATGTAACCTTTAACGATGCTTATACTTTCT
TACCTTTCGGGAATACGTTATATACCTATAAAATGGAAGGTTCGTTAGTGAAACAAGTGCTTGAAGATGCAATGCAATTT
GCTTTGGTTGATGGCTCTACAGGCGCATTCCCTTATGGTGCAGGTATTCGTTATGAAGCGAATGAAACACCAAATGCGGA
AGGTAAGCGTTTAGTGAGTGTTGAAGTCTTGAATAAACAAACCCAACAATGGGAACCGATTGATGATAACAAACGTTATC
TTGTCGGAACAAATGCTTATGTTGCAGGCGGTAAAGACGGTTATAAAACCTTTGGTAAATTATTTAACGATCCAAAATAT
GAAGGCGTTGATACCTACTTACCTGATGCGGAAAGCTTCATTAAATTTATGAAAAAACACCCGCACTTTGAGGCTTATAC
TTCATCAAATGTGAAATTTAATGTTTCAACTGATGCATTACCTAAAAAATAA

Upstream 100 bases:

>100_bases
CCTCGAGAAAATTTACAGCACAATAATGATGCAATTCGTTACAAAACCTTTCAAGCAATGCTAGAAGGTTTAAACTAATC
CCTTACCAATAGGAGTTTTT

Downstream 100 bases:

>100_bases
AATTGAAAAATAGGCTAAAAATAGCGCGTATTTAACGCGCTATTTTTGTTATTAGTCCTAGGTTTGAAGACTTCATAGAT
GATTAAGAATTTTAACTATT

Product: NAD nucleotidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MLLSKKSATFALSAFAMLFTSVALAKEAPQAHKAVELSILHINDHHSYLEPHETRINLNGQQTKVDIGGFSAVNAKLNKL
RKKYKNPLVLHAGDAITGTLYFTLFGGSADAAVMNAGNFHYFTLGNHEFDAGNEGLLKLLEPLKIPVLSANVIPDKNSIL
YNKWKPYDIFTVDGEKIAIIGLDTVNKTVNSSSPGKDVKFYDEIATAQIMANALKQQGINKIILLSHAGSEKNIEIAQKV
NDIDVIVTGDSHYLYGNDELRSLKLPVIYEYPLEFKNPNGEPVFVMEGWAYSAVVGDLGVKFSPEGIASITRKIPHVLMS
SHKLQVKNSEGKWAELTGDERKKALDTLKSMKSISLDDHDAKTDMLISKYKSEKDRLAQEIVGVITGSAMPGGSANRIPN
KAGSNPEGSIATRFIAETMYNELKTVDLTIQNAGGVRADILPGNVTFNDAYTFLPFGNTLYTYKMEGSLVKQVLEDAMQF
ALVDGSTGAFPYGAGIRYEANETPNAEGKRLVSVEVLNKQTQQWEPIDDNKRYLVGTNAYVAGGKDGYKTFGKLFNDPKY
EGVDTYLPDAESFIKFMKKHPHFEAYTSSNVKFNVSTDALPKK

Sequences:

>Translated_603_residues
MLLSKKSATFALSAFAMLFTSVALAKEAPQAHKAVELSILHINDHHSYLEPHETRINLNGQQTKVDIGGFSAVNAKLNKL
RKKYKNPLVLHAGDAITGTLYFTLFGGSADAAVMNAGNFHYFTLGNHEFDAGNEGLLKLLEPLKIPVLSANVIPDKNSIL
YNKWKPYDIFTVDGEKIAIIGLDTVNKTVNSSSPGKDVKFYDEIATAQIMANALKQQGINKIILLSHAGSEKNIEIAQKV
NDIDVIVTGDSHYLYGNDELRSLKLPVIYEYPLEFKNPNGEPVFVMEGWAYSAVVGDLGVKFSPEGIASITRKIPHVLMS
SHKLQVKNSEGKWAELTGDERKKALDTLKSMKSISLDDHDAKTDMLISKYKSEKDRLAQEIVGVITGSAMPGGSANRIPN
KAGSNPEGSIATRFIAETMYNELKTVDLTIQNAGGVRADILPGNVTFNDAYTFLPFGNTLYTYKMEGSLVKQVLEDAMQF
ALVDGSTGAFPYGAGIRYEANETPNAEGKRLVSVEVLNKQTQQWEPIDDNKRYLVGTNAYVAGGKDGYKTFGKLFNDPKY
EGVDTYLPDAESFIKFMKKHPHFEAYTSSNVKFNVSTDALPKK
>Mature_603_residues
MLLSKKSATFALSAFAMLFTSVALAKEAPQAHKAVELSILHINDHHSYLEPHETRINLNGQQTKVDIGGFSAVNAKLNKL
RKKYKNPLVLHAGDAITGTLYFTLFGGSADAAVMNAGNFHYFTLGNHEFDAGNEGLLKLLEPLKIPVLSANVIPDKNSIL
YNKWKPYDIFTVDGEKIAIIGLDTVNKTVNSSSPGKDVKFYDEIATAQIMANALKQQGINKIILLSHAGSEKNIEIAQKV
NDIDVIVTGDSHYLYGNDELRSLKLPVIYEYPLEFKNPNGEPVFVMEGWAYSAVVGDLGVKFSPEGIASITRKIPHVLMS
SHKLQVKNSEGKWAELTGDERKKALDTLKSMKSISLDDHDAKTDMLISKYKSEKDRLAQEIVGVITGSAMPGGSANRIPN
KAGSNPEGSIATRFIAETMYNELKTVDLTIQNAGGVRADILPGNVTFNDAYTFLPFGNTLYTYKMEGSLVKQVLEDAMQF
ALVDGSTGAFPYGAGIRYEANETPNAEGKRLVSVEVLNKQTQQWEPIDDNKRYLVGTNAYVAGGKDGYKTFGKLFNDPKY
EGVDTYLPDAESFIKFMKKHPHFEAYTSSNVKFNVSTDALPKK

Specific function: Degradation Of External Udp-Glucose To Uridine Monophosphate And Glucose-1-Phosphate, Which Can Then Be Used By The Cell. [C]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=548, Percent_Identity=29.7445255474453, Blast_Score=164, Evalue=3e-40,
Organism=Escherichia coli, GI1786687, Length=239, Percent_Identity=30.1255230125523, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922446, Length=540, Percent_Identity=27.962962962963, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24654424, Length=540, Percent_Identity=27.962962962963, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19922444, Length=606, Percent_Identity=26.5676567656766, Blast_Score=149, Evalue=8e-36,
Organism=Drosophila melanogaster, GI28573524, Length=575, Percent_Identity=26.9565217391304, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24641187, Length=532, Percent_Identity=27.2556390977444, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI221329836, Length=557, Percent_Identity=26.032315978456, Blast_Score=136, Evalue=5e-32,
Organism=Drosophila melanogaster, GI161076508, Length=219, Percent_Identity=27.8538812785388, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921980, Length=219, Percent_Identity=27.8538812785388, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652512, Length=219, Percent_Identity=27.8538812785388, Blast_Score=82, Evalue=2e-15,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843
- InterPro:   IPR006420 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 66288; Mature: 66288

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSKKSATFALSAFAMLFTSVALAKEAPQAHKAVELSILHINDHHSYLEPHETRINLNG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEECC
QQTKVDIGGFSAVNAKLNKLRKKYKNPLVLHAGDAITGTLYFTLFGGSADAAVMNAGNFH
CEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEECCCCCEEEEECCCEE
YFTLGNHEFDAGNEGLLKLLEPLKIPVLSANVIPDKNSILYNKWKPYDIFTVDGEKIAII
EEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCEEEEEECCCEEEEE
GLDTVNKTVNSSSPGKDVKFYDEIATAQIMANALKQQGINKIILLSHAGSEKNIEIAQKV
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEHEECC
NDIDVIVTGDSHYLYGNDELRSLKLPVIYEYPLEFKNPNGEPVFVMEGWAYSAVVGDLGV
CCEEEEEECCCEEEECCCCCEEEECCEEEECCCEECCCCCCEEEEECCCEEEEHHHCCCC
KFSPEGIASITRKIPHVLMSSHKLQVKNSEGKWAELTGDERKKALDTLKSMKSISLDDHD
EECCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCC
AKTDMLISKYKSEKDRLAQEIVGVITGSAMPGGSANRIPNKAGSNPEGSIATRFIAETMY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NELKTVDLTIQNAGGVRADILPGNVTFNDAYTFLPFGNTLYTYKMEGSLVKQVLEDAMQF
HCCEEEEEEEECCCCEEEEEECCCEEECCCEEEEECCCEEEEEEECHHHHHHHHHHHHHE
ALVDGSTGAFPYGAGIRYEANETPNAEGKRLVSVEVLNKQTQQWEPIDDNKRYLVGTNAY
EEECCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCHHHCCCCCCCCCCEEEECCEE
VAGGKDGYKTFGKLFNDPKYEGVDTYLPDAESFIKFMKKHPHFEAYTSSNVKFNVSTDAL
EECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEECCCCC
PKK
CCC
>Mature Secondary Structure
MLLSKKSATFALSAFAMLFTSVALAKEAPQAHKAVELSILHINDHHSYLEPHETRINLNG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEECC
QQTKVDIGGFSAVNAKLNKLRKKYKNPLVLHAGDAITGTLYFTLFGGSADAAVMNAGNFH
CEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEECCCCCEEEEECCCEE
YFTLGNHEFDAGNEGLLKLLEPLKIPVLSANVIPDKNSILYNKWKPYDIFTVDGEKIAII
EEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCEEEEEECCCEEEEE
GLDTVNKTVNSSSPGKDVKFYDEIATAQIMANALKQQGINKIILLSHAGSEKNIEIAQKV
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEHEECC
NDIDVIVTGDSHYLYGNDELRSLKLPVIYEYPLEFKNPNGEPVFVMEGWAYSAVVGDLGV
CCEEEEEECCCEEEECCCCCEEEECCEEEECCCEECCCCCCEEEEECCCEEEEHHHCCCC
KFSPEGIASITRKIPHVLMSSHKLQVKNSEGKWAELTGDERKKALDTLKSMKSISLDDHD
EECCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCC
AKTDMLISKYKSEKDRLAQEIVGVITGSAMPGGSANRIPNKAGSNPEGSIATRFIAETMY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NELKTVDLTIQNAGGVRADILPGNVTFNDAYTFLPFGNTLYTYKMEGSLVKQVLEDAMQF
HCCEEEEEEEECCCCEEEEEECCCEEECCCEEEEECCCEEEEEEECHHHHHHHHHHHHHE
ALVDGSTGAFPYGAGIRYEANETPNAEGKRLVSVEVLNKQTQQWEPIDDNKRYLVGTNAY
EEECCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCHHHCCCCCCCCCCEEEECCEE
VAGGKDGYKTFGKLFNDPKYEGVDTYLPDAESFIKFMKKHPHFEAYTSSNVKFNVSTDAL
EECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEECCCCC
PKK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]