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The map label for this gene is nagK [H]

Identifier: 148827321

GI number: 148827321

Start: 652700

End: 653614

Strand: Direct

Name: nagK [H]

Synonym: CGSHiGG_03510

Alternate gene names: 148827321

Gene position: 652700-653614 (Clockwise)

Preceding gene: 148827315

Following gene: 148827322

Centisome position: 34.59

GC content: 40.0

Gene sequence:

>915_bases
ATGTATTATGGTTTAGATATTGGTGGCACGAAAATAGAGCTTGCCGTATTCAATGAAAAATTAGAAAAATTATATTCTGA
ACGGGTACCAACACCTAAAACCGACTATGAAGAATGGCTTAACACGATTGTGGATTTAGTGAATCGTGCTGATGAAAAGT
TTGGTGAGGCGGGCACCGTAGGTTTGGGCGTACCTGGTTTTGTTAATCAACAAACTGGATTAGCCGAAATTGCCAATATT
AGTGTGGCAGATAATAAACCAATTTTATGCGATTTGTCCGCGCGTTTGGGCCGTGAAGTGCGGGCAGAAAATGACGCAAA
TTGTTTTGCGTTATCTGAAGCATGGGATACGGAAAATCAGCAATATTCGACTGTACTTGGTTTAATTCTTGGGACTGGTT
TTGGTGGTGGCTTTGTCTTAAACGGTAAAGTTCATTCAGGTCAAGTGGGAATGGCGGGAGAGCTTGGTCATTTACAATTA
AATTATCATGCTTTGAAATTATTGGGATGGGATAACGCCCCAATTTATCAATGCGGTTGCGGCAACAAGGCTTGTTTGGA
TAATTATCTGTCTGGTCGCGGTTTTGAAATGCTTTATCAAGATTTGAAAGGCGAAACATTATCTGCTCGTAAAATTATTG
ATTTGTTCTATCAAAGCAATGAAAGTGCGGTAGATTTTGTGAATCTTTTTGTTGAATTGGCGGCGATTTCTATTGGCAAT
ATCATTACGGCATTTGATCCTCATATGATTGTGTTAGGTGGTGGATTATCTAATTTTGATTACCTTTATGAGGCTTTACC
AAAAGCGCTTCCGCCTCATTTGATGCGAAAAGCAAAAGTTCCGCCAATTAAAAAAGCGAAACACGGCGATTCTGGTGGTG
TGCGTGGTGCGGCAGCGTTGTTTTTAACCAAATAA

Upstream 100 bases:

>100_bases
CTTTTTGAAGAAAAAGGCGATCTTTGTCATAAAATAATGATTTAAGTGCGGTTAAAATTTTGCCTAATTTTAACCGCACT
TCTTTATACAGGAGCAGATT

Downstream 100 bases:

>100_bases
TTCAACAAACTAGTGGGGCGTATTCAAATAATACGCCCTTTATTTTGGAAAATATTTAGAATATTGTGAATTTATTAGAT
TTTTTCTTGTTTGTGTTTCT

Product: N-acetyl-D-glucosamine kinase

Products: NA

Alternate protein names: GlcNAc kinase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEAGTVGLGVPGFVNQQTGLAEIANI
SVADNKPILCDLSARLGREVRAENDANCFALSEAWDTENQQYSTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQL
NYHALKLLGWDNAPIYQCGCGNKACLDNYLSGRGFEMLYQDLKGETLSARKIIDLFYQSNESAVDFVNLFVELAAISIGN
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRKAKVPPIKKAKHGDSGGVRGAAALFLTK

Sequences:

>Translated_304_residues
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEAGTVGLGVPGFVNQQTGLAEIANI
SVADNKPILCDLSARLGREVRAENDANCFALSEAWDTENQQYSTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQL
NYHALKLLGWDNAPIYQCGCGNKACLDNYLSGRGFEMLYQDLKGETLSARKIIDLFYQSNESAVDFVNLFVELAAISIGN
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRKAKVPPIKKAKHGDSGGVRGAAALFLTK
>Mature_304_residues
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEAGTVGLGVPGFVNQQTGLAEIANI
SVADNKPILCDLSARLGREVRAENDANCFALSEAWDTENQQYSTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQL
NYHALKLLGWDNAPIYQCGCGNKACLDNYLSGRGFEMLYQDLKGETLSARKIIDLFYQSNESAVDFVNLFVELAAISIGN
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRKAKVPPIKKAKHGDSGGVRGAAALFLTK

Specific function: Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NagK subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787363, Length=303, Percent_Identity=50.1650165016502, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI87081733, Length=302, Percent_Identity=41.0596026490066, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI1790522, Length=314, Percent_Identity=26.1146496815287, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.59 [H]

Molecular weight: Translated: 32981; Mature: 32981

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS01125 ROK ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEAGTV
CEEEEECCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
GLGVPGFVNQQTGLAEIANISVADNKPILCDLSARLGREVRAENDANCFALSEAWDTENQ
CCCCCCCCCCCCCHHHHHEEEECCCCCEEEECHHHHCCHHCCCCCCCEEEEECCCCCCCH
QYSTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQLNYHALKLLGWDNAPIYQCGC
HHHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCEEEEEEEEEEEEECCCCCEEEECC
GNKACLDNYLSGRGFEMLYQDLKGETLSARKIIDLFYQSNESAVDFVNLFVELAAISIGN
CCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRKAKVPPIKKAKHGDSGGVRGAAAL
EEEECCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEE
FLTK
EEEC
>Mature Secondary Structure
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEAGTV
CEEEEECCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
GLGVPGFVNQQTGLAEIANISVADNKPILCDLSARLGREVRAENDANCFALSEAWDTENQ
CCCCCCCCCCCCCHHHHHEEEECCCCCEEEECHHHHCCHHCCCCCCCEEEEECCCCCCCH
QYSTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQLNYHALKLLGWDNAPIYQCGC
HHHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCEEEEEEEEEEEEECCCCCEEEECC
GNKACLDNYLSGRGFEMLYQDLKGETLSARKIIDLFYQSNESAVDFVNLFVELAAISIGN
CCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRKAKVPPIKKAKHGDSGGVRGAAAL
EEEECCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEE
FLTK
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA