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The map label for this gene is dapE [H]

Identifier: 148827243

GI number: 148827243

Start: 556633

End: 557766

Strand: Reverse

Name: dapE [H]

Synonym: CGSHiGG_03055

Alternate gene names: 148827243

Gene position: 557766-556633 (Counterclockwise)

Preceding gene: 148827244

Following gene: 148827242

Centisome position: 29.56

GC content: 40.92

Gene sequence:

>1134_bases
ATGAGAGAAAAAGTGGTTTCGTTGGCGCAAGATTTAATTCATCGCTCATCTATTAGCCCGAATGACGAAGGCTGTCAGAA
AATTATTGCCGAACGTTTAGAAAAATTAGGTTTTCAAATTGAATGGATGCCTTTTAATGACACGTTGAATTTGTGGGCAA
AACACGGAACGAGCGAGCCAGTTATTGCTTTTGCAGGTCATACGGATGTTGTGCCTACTGGCGATGAAAATCAATGGTCG
TCTCCGCCGTTTTCTGCGGAGATTATTGATGGCATGCTTTATGGGCGTGGTGCGGCGGATATGAAAGGCTCCCTTGCAGC
AATGATTGTGGCGGCAGAAGAATACGTAAAAGCAAATCCTAACCATAAAGGCATCATTGCATTATTGATTACCTCTGATG
AAGAGGCTGCCGCAAAAGATGGAACTATACGCGTGGTTGAGACGTTAATGACGCGTGATGAAAAAATTACTTATTGTATG
GTTGGCGAGCCATCGAGTGCTAAAAACTTAGGCGATGTTGTCAAAAATGGTCGCCGCGGCTCAATTACAGGAAATCTCTA
TATTCAAGGTATTCAAGGACACGTAGCCTATCCGCATTTAGCTGAAAACCCAATCCATAAAGCAGCACTATTTTTGCAAG
AATTAACGACTTACCAATGGGATAAAGGCAATGAATTTTTCCCACCAACAAGCCTACAAATTGCTAACATTCACGCGGGT
ACGGGAAGTAATAATGTAATTCCTGCTGAGTTATATATTCAGTTCAATTTGCGTTATTGCACAGAAGTCACGGATGAAAT
CATTAAGCAAAAAGTGGCTGAAATGCTAGAAAAACACAATTTGAAATATCGTATTGAATGGAATTTATCAGGCAACCCCT
TTTTAACAAAACCAGGTAAATTATTAGATTCGATAACCTCCGCTATTGAGGAAACCATAGGCATAACGCCAAAGGCTGAA
ACAGGTGGTGGCACGTCAGACGGTCGTTTTATTGCATTGATGGGCGCAGAAGTAGTGGAATTTGGTCCGTTAAATTCAAC
CATTCATAAAGTCAATGAATGTGTCAGTGTAGAAGACCTTGGCAAGTGCGGTGAAATTTACCACAAAATGTTAGTGAATT
TATTGGATAGCTAA

Upstream 100 bases:

>100_bases
CCCAATTATTTTACAAGATGGAAAGGCATTAATTGGATTTAATGAAAAGGAATATCAGGCTGCTTTTGCTTAAAGTGCGG
TTATTTTCTTAGGAGTTTTT

Downstream 100 bases:

>100_bases
AATGAAATTAACCCCAGAAATGCTTACAGGAAAGTCTCGTGAGCATTTAGTCAATTTACCCACAGCTCATTCATCAAATC
ATTTTTTGCAAACGCAAGCT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MREKVVSLAQDLIHRSSISPNDEGCQKIIAERLEKLGFQIEWMPFNDTLNLWAKHGTSEPVIAFAGHTDVVPTGDENQWS
SPPFSAEIIDGMLYGRGAADMKGSLAAMIVAAEEYVKANPNHKGIIALLITSDEEAAAKDGTIRVVETLMTRDEKITYCM
VGEPSSAKNLGDVVKNGRRGSITGNLYIQGIQGHVAYPHLAENPIHKAALFLQELTTYQWDKGNEFFPPTSLQIANIHAG
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLEKHNLKYRIEWNLSGNPFLTKPGKLLDSITSAIEETIGITPKAE
TGGGTSDGRFIALMGAEVVEFGPLNSTIHKVNECVSVEDLGKCGEIYHKMLVNLLDS

Sequences:

>Translated_377_residues
MREKVVSLAQDLIHRSSISPNDEGCQKIIAERLEKLGFQIEWMPFNDTLNLWAKHGTSEPVIAFAGHTDVVPTGDENQWS
SPPFSAEIIDGMLYGRGAADMKGSLAAMIVAAEEYVKANPNHKGIIALLITSDEEAAAKDGTIRVVETLMTRDEKITYCM
VGEPSSAKNLGDVVKNGRRGSITGNLYIQGIQGHVAYPHLAENPIHKAALFLQELTTYQWDKGNEFFPPTSLQIANIHAG
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLEKHNLKYRIEWNLSGNPFLTKPGKLLDSITSAIEETIGITPKAE
TGGGTSDGRFIALMGAEVVEFGPLNSTIHKVNECVSVEDLGKCGEIYHKMLVNLLDS
>Mature_377_residues
MREKVVSLAQDLIHRSSISPNDEGCQKIIAERLEKLGFQIEWMPFNDTLNLWAKHGTSEPVIAFAGHTDVVPTGDENQWS
SPPFSAEIIDGMLYGRGAADMKGSLAAMIVAAEEYVKANPNHKGIIALLITSDEEAAAKDGTIRVVETLMTRDEKITYCM
VGEPSSAKNLGDVVKNGRRGSITGNLYIQGIQGHVAYPHLAENPIHKAALFLQELTTYQWDKGNEFFPPTSLQIANIHAG
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLEKHNLKYRIEWNLSGNPFLTKPGKLLDSITSAIEETIGITPKAE
TGGGTSDGRFIALMGAEVVEFGPLNSTIHKVNECVSVEDLGKCGEIYHKMLVNLLDS

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=60.8, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1790395, Length=259, Percent_Identity=28.957528957529, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789236, Length=401, Percent_Identity=22.9426433915212, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17551016, Length=215, Percent_Identity=26.5116279069767, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41370; Mature: 41370

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREKVVSLAQDLIHRSSISPNDEGCQKIIAERLEKLGFQIEWMPFNDTLNLWAKHGTSEP
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCCC
VIAFAGHTDVVPTGDENQWSSPPFSAEIIDGMLYGRGAADMKGSLAAMIVAAEEYVKANP
EEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHEEEHHHHHHCCC
NHKGIIALLITSDEEAAAKDGTIRVVETLMTRDEKITYCMVGEPSSAKNLGDVVKNGRRG
CCCCEEEEEEECCCHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCC
SITGNLYIQGIQGHVAYPHLAENPIHKAALFLQELTTYQWDKGNEFFPPTSLQIANIHAG
CEEEEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECC
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLEKHNLKYRIEWNLSGNPFLTKPGK
CCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHH
LLDSITSAIEETIGITPKAETGGGTSDGRFIALMGAEVVEFGPLNSTIHKVNECVSVEDL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHCCHHHH
GKCGEIYHKMLVNLLDS
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MREKVVSLAQDLIHRSSISPNDEGCQKIIAERLEKLGFQIEWMPFNDTLNLWAKHGTSEP
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCCC
VIAFAGHTDVVPTGDENQWSSPPFSAEIIDGMLYGRGAADMKGSLAAMIVAAEEYVKANP
EEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHEEEHHHHHHCCC
NHKGIIALLITSDEEAAAKDGTIRVVETLMTRDEKITYCMVGEPSSAKNLGDVVKNGRRG
CCCCEEEEEEECCCHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCC
SITGNLYIQGIQGHVAYPHLAENPIHKAALFLQELTTYQWDKGNEFFPPTSLQIANIHAG
CEEEEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECC
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLEKHNLKYRIEWNLSGNPFLTKPGK
CCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHH
LLDSITSAIEETIGITPKAETGGGTSDGRFIALMGAEVVEFGPLNSTIHKVNECVSVEDL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHCCHHHH
GKCGEIYHKMLVNLLDS
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA