Click here to switch to the map view.

The map label for this gene is amiB [H]

Identifier: 148827219

GI number: 148827219

Start: 523075

End: 524373

Strand: Direct

Name: amiB [H]

Synonym: CGSHiGG_02845

Alternate gene names: 148827219

Gene position: 523075-524373 (Clockwise)

Preceding gene: 148827218

Following gene: 148827220

Centisome position: 27.72

GC content: 32.64

Gene sequence:

>1299_bases
ATGAAAACTAAAATTTTATTTTTTCTTTTTTTCTCGACATTTAGCTTTTCTATTTTTGCAGCTCCTATTACGATTGCAAT
TGACCCTGGTCATGGGGGAAAAGATCCTGGTGCAATCGGCCGAAACTTAGGCATTTATGAAAAAAATGTTACCCTTTCAA
TTGCAAAAGAATTAAAAGCCTTATTAGATAAAGATCCACATTTTCGTGGTGTATTAACACGTAAAAGTGATTATTATATT
TCCGTGCCAGAACGTTCAGAAATCGCACGTAAATTTAAAGCGAATTATCTTATTTCCATTCATGCGGATTCATCTGAATC
GCCTGATCGTCGCGGGGCTTCTGTTTGGGTATTGTCAAACCGTCGAGCCAATGATGAAATGGGTCAATGGCTAGAAGACG
ATGAAAAACGCTCGGAATTACTTGGTGGAGCGGGAAAGGTGCTTTCACACAATAATGACAAATATCTTGACCAGACTGTA
CTTGATCTGCAATTCGGACATAGCCAACGTACAGGCTATGTGTTAGGCGAACATATTTTGCACCATTTTGCTAAAGTAAC
AACATTAAGTCGCAGTACGCCACAACATGCAAGTCTGGGTGTTCTACGTTCGCCAGATATTCCTTCTGTGTTGGTAGAAA
CGGGATTTCTTTCCAATTCAGAAGAAGAAAAAAAACTTCATTCCCAAACTTATCGTCGCCGCATTGCTTATATGATTTAT
GAAGGCTTAGTTGCATTCCATAGCGGTAAAACCAACACTTTAGTTAAAGATAATTTAGTGCAAAACATCAAACAAAACGA
TATAAAAAAATCAGGGAAAAATAACCGCACTTCAGAACAAAATATAAATGAAGATAATATTAAAGACAGTGGTATTCGCC
ATATTGTAAAAAAAGGAGAAAGTTTAGGTAGCTTATCAAATAAATATCACGTAAAAGTCTCTGATATTATTAAATTAAAT
CAATTAAAGAGAAAAACACTTTGGCTAAATGAAAGTATTAAAATCCCTGATAATGTCGAGATAAAAAATAAATCATTAAC
AATTAAAGAAAATGATTTTCATAAAAAACAAAATAGCTCAGTAAATAATACCAATAAAGATTTAAAAAAAGAGAAAAATA
CACAAACTAATAATCAAAAAAATATAATTCCGCTCTATCATAAAGTAACAAAAAACCAAACACTTTATGCTATTTCGAGG
GAATATAACATTCCAGTAAATATACTATTGAGCCTAAATCCACATTTAAAAAATGGCAAAGTTATTACAGGACAAAAAAT
AAAATTAAGAGAAAAATAA

Upstream 100 bases:

>100_bases
GTGAATATTGATTATTACGATGATGCACGAAATATTGAATTAATCGCACAAACAAATTTGGGTAAAAATATTATAAGCGC
ATTTTCTAATTAACTTATTT

Downstream 100 bases:

>100_bases
ATGCCTATTAAAATTCTTTCCCCACAACTTGCTAACCAAATTGCTGCTGGAGAAGTGGTAGAACGCCCTGCATCAGTGGT
AAAAGAATTAGTGGAAAATA

Product: lipid ABC transporter membrane protein/ATP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKTKILFFLFFSTFSFSIFAAPITIAIDPGHGGKDPGAIGRNLGIYEKNVTLSIAKELKALLDKDPHFRGVLTRKSDYYI
SVPERSEIARKFKANYLISIHADSSESPDRRGASVWVLSNRRANDEMGQWLEDDEKRSELLGGAGKVLSHNNDKYLDQTV
LDLQFGHSQRTGYVLGEHILHHFAKVTTLSRSTPQHASLGVLRSPDIPSVLVETGFLSNSEEEKKLHSQTYRRRIAYMIY
EGLVAFHSGKTNTLVKDNLVQNIKQNDIKKSGKNNRTSEQNINEDNIKDSGIRHIVKKGESLGSLSNKYHVKVSDIIKLN
QLKRKTLWLNESIKIPDNVEIKNKSLTIKENDFHKKQNSSVNNTNKDLKKEKNTQTNNQKNIIPLYHKVTKNQTLYAISR
EYNIPVNILLSLNPHLKNGKVITGQKIKLREK

Sequences:

>Translated_432_residues
MKTKILFFLFFSTFSFSIFAAPITIAIDPGHGGKDPGAIGRNLGIYEKNVTLSIAKELKALLDKDPHFRGVLTRKSDYYI
SVPERSEIARKFKANYLISIHADSSESPDRRGASVWVLSNRRANDEMGQWLEDDEKRSELLGGAGKVLSHNNDKYLDQTV
LDLQFGHSQRTGYVLGEHILHHFAKVTTLSRSTPQHASLGVLRSPDIPSVLVETGFLSNSEEEKKLHSQTYRRRIAYMIY
EGLVAFHSGKTNTLVKDNLVQNIKQNDIKKSGKNNRTSEQNINEDNIKDSGIRHIVKKGESLGSLSNKYHVKVSDIIKLN
QLKRKTLWLNESIKIPDNVEIKNKSLTIKENDFHKKQNSSVNNTNKDLKKEKNTQTNNQKNIIPLYHKVTKNQTLYAISR
EYNIPVNILLSLNPHLKNGKVITGQKIKLREK
>Mature_432_residues
MKTKILFFLFFSTFSFSIFAAPITIAIDPGHGGKDPGAIGRNLGIYEKNVTLSIAKELKALLDKDPHFRGVLTRKSDYYI
SVPERSEIARKFKANYLISIHADSSESPDRRGASVWVLSNRRANDEMGQWLEDDEKRSELLGGAGKVLSHNNDKYLDQTV
LDLQFGHSQRTGYVLGEHILHHFAKVTTLSRSTPQHASLGVLRSPDIPSVLVETGFLSNSEEEKKLHSQTYRRRIAYMIY
EGLVAFHSGKTNTLVKDNLVQNIKQNDIKKSGKNNRTSEQNINEDNIKDSGIRHIVKKGESLGSLSNKYHVKVSDIIKLN
QLKRKTLWLNESIKIPDNVEIKNKSLTIKENDFHKKQNSSVNNTNKDLKKEKNTQTNNQKNIIPLYHKVTKNQTLYAISR
EYNIPVNILLSLNPHLKNGKVITGQKIKLREK

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=222, Percent_Identity=59.9099099099099, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI87082163, Length=231, Percent_Identity=36.7965367965368, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788776, Length=222, Percent_Identity=40.0900900900901, Blast_Score=159, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01520 Amidase_3; PF01476 LysM [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 49078; Mature: 49078

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKILFFLFFSTFSFSIFAAPITIAIDPGHGGKDPGAIGRNLGIYEKNVTLSIAKELKA
CCCEEEEEEEHHHHCCEEEEEEEEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHH
LLDKDPHFRGVLTRKSDYYISVPERSEIARKFKANYLISIHADSSESPDRRGASVWVLSN
HHCCCCCCEEEEEECCCEEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEC
RRANDEMGQWLEDDEKRSELLGGAGKVLSHNNDKYLDQTVLDLQFGHSQRTGYVLGEHIL
CCCCHHHHHHHCCHHHHHHHHCCCCHHHCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHH
HHFAKVTTLSRSTPQHASLGVLRSPDIPSVLVETGFLSNSEEEKKLHSQTYRRRIAYMIY
HHHHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EGLVAFHSGKTNTLVKDNLVQNIKQNDIKKSGKNNRTSEQNINEDNIKDSGIRHIVKKGE
HHHHHEECCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
SLGSLSNKYHVKVSDIIKLNQLKRKTLWLNESIKIPDNVEIKNKSLTIKENDFHKKQNSS
HHHCCCCEEEEEHHHHHHHHHHHHHHEEECCCCCCCCCCEEECCEEEEECCCCCHHCCCC
VNNTNKDLKKEKNTQTNNQKNIIPLYHKVTKNQTLYAISREYNIPVNILLSLNPHLKNGK
CCCCHHHHHHHCCCCCCCCCCEEEHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCE
VITGQKIKLREK
EEECCEEEECCC
>Mature Secondary Structure
MKTKILFFLFFSTFSFSIFAAPITIAIDPGHGGKDPGAIGRNLGIYEKNVTLSIAKELKA
CCCEEEEEEEHHHHCCEEEEEEEEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHH
LLDKDPHFRGVLTRKSDYYISVPERSEIARKFKANYLISIHADSSESPDRRGASVWVLSN
HHCCCCCCEEEEEECCCEEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEC
RRANDEMGQWLEDDEKRSELLGGAGKVLSHNNDKYLDQTVLDLQFGHSQRTGYVLGEHIL
CCCCHHHHHHHCCHHHHHHHHCCCCHHHCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHH
HHFAKVTTLSRSTPQHASLGVLRSPDIPSVLVETGFLSNSEEEKKLHSQTYRRRIAYMIY
HHHHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EGLVAFHSGKTNTLVKDNLVQNIKQNDIKKSGKNNRTSEQNINEDNIKDSGIRHIVKKGE
HHHHHEECCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
SLGSLSNKYHVKVSDIIKLNQLKRKTLWLNESIKIPDNVEIKNKSLTIKENDFHKKQNSS
HHHCCCCEEEEEHHHHHHHHHHHHHHEEECCCCCCCCCCEEECCEEEEECCCCCHHCCCC
VNNTNKDLKKEKNTQTNNQKNIIPLYHKVTKNQTLYAISREYNIPVNILLSLNPHLKNGK
CCCCHHHHHHHCCCCCCCCCCEEEHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCE
VITGQKIKLREK
EEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]