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The map label for this gene is lipB [H]

Identifier: 148827185

GI number: 148827185

Start: 482034

End: 482672

Strand: Reverse

Name: lipB [H]

Synonym: CGSHiGG_02620

Alternate gene names: 148827185

Gene position: 482672-482034 (Counterclockwise)

Preceding gene: 148827186

Following gene: 148827184

Centisome position: 25.58

GC content: 37.72

Gene sequence:

>639_bases
ATGAACAATTCCCTTATCGTTCGTCAGTTAGGATTGCAAGATTATCAAGAAATTTGGCACAAAATGCAGGATTTTACTGA
TACTCGAAATGCTGAAACGCAAGACGAAATTTGGTTAGTACAACATTATCCTGTTTTTACCCAAGGGCAAGCAGGTAAAC
CAGAGCATCTTTTACAACGTAGTGAAATCCCTGTGGTGCAATCGGATCGTGGTGGGCAAATTACCTATCACGCACCTGGT
CAGCAAGTGATGTATGTGCTTATTGACATTAAACGCCATAAAAATTTAAATGTACGTCAATTAGTTACCGCACTTGAACA
AACGGTGGTTAAAACTTTGGCTGAATATGGCATTGAAAGCTATCCAAAGCCTGATGCGCCAGGTGTTTATGTGGATGGAA
AAAAGATTTGTTCTCTTGGATTACGTATTCGTCGTGGTTGCTCTTTTCACGGGTTGGCATTGAATATTAATATGGATCTT
AATCCTTTCCATTATATTAATCCTTGTGGTTATGCAGGGCTTGAAATGTGTCAGTTAGCGGATTTTGTTAATCAAGATGA
AGCTGATTGGGATAATGTTTCAGCAAAATTAATTAAACACTTCGCCGATCTTTTAGGCTATAATATAACAACTTTATAA

Upstream 100 bases:

>100_bases
AATTCAGTTTCAATTGATATTATTGCTGAAAACTTTGATCAGGTAGAAACGCTCTATAAAGAACTGGCTAAAGTTGAAGG
CGTGAAAATGGTTATTTAGT

Downstream 100 bases:

>100_bases
CATTTTATAAAAACTAAATGCCCAATTTGGGCATTTTTATTTAGGAAAATACTATGTCTACGCCTTTCAAAATGGAACGC
GGTGTAAAATATCGCGATGC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNNSLIVRQLGLQDYQEIWHKMQDFTDTRNAETQDEIWLVQHYPVFTQGQAGKPEHLLQRSEIPVVQSDRGGQITYHAPG
QQVMYVLIDIKRHKNLNVRQLVTALEQTVVKTLAEYGIESYPKPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNINMDL
NPFHYINPCGYAGLEMCQLADFVNQDEADWDNVSAKLIKHFADLLGYNITTL

Sequences:

>Translated_212_residues
MNNSLIVRQLGLQDYQEIWHKMQDFTDTRNAETQDEIWLVQHYPVFTQGQAGKPEHLLQRSEIPVVQSDRGGQITYHAPG
QQVMYVLIDIKRHKNLNVRQLVTALEQTVVKTLAEYGIESYPKPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNINMDL
NPFHYINPCGYAGLEMCQLADFVNQDEADWDNVSAKLIKHFADLLGYNITTL
>Mature_212_residues
MNNSLIVRQLGLQDYQEIWHKMQDFTDTRNAETQDEIWLVQHYPVFTQGQAGKPEHLLQRSEIPVVQSDRGGQITYHAPG
QQVMYVLIDIKRHKNLNVRQLVTALEQTVVKTLAEYGIESYPKPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNINMDL
NPFHYINPCGYAGLEMCQLADFVNQDEADWDNVSAKLIKHFADLLGYNITTL

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=179, Percent_Identity=31.2849162011173, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI87081767, Length=203, Percent_Identity=65.5172413793103, Blast_Score=280, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6323268, Length=189, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355483, Length=180, Percent_Identity=31.6666666666667, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24159; Mature: 24159

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSLIVRQLGLQDYQEIWHKMQDFTDTRNAETQDEIWLVQHYPVFTQGQAGKPEHLLQR
CCCCEEEHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCCCCCHHHHHHH
SEIPVVQSDRGGQITYHAPGQQVMYVLIDIKRHKNLNVRQLVTALEQTVVKTLAEYGIES
CCCCEEECCCCCEEEEECCCHHEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
YPKPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNINMDLNPFHYINPCGYAGLEMCQLA
CCCCCCCCEEECHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHHH
DFVNQDEADWDNVSAKLIKHFADLLGYNITTL
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNNSLIVRQLGLQDYQEIWHKMQDFTDTRNAETQDEIWLVQHYPVFTQGQAGKPEHLLQR
CCCCEEEHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCCCCCHHHHHHH
SEIPVVQSDRGGQITYHAPGQQVMYVLIDIKRHKNLNVRQLVTALEQTVVKTLAEYGIES
CCCCEEECCCCCEEEEECCCHHEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
YPKPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNINMDLNPFHYINPCGYAGLEMCQLA
CCCCCCCCEEECHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCHHHHHHHHH
DFVNQDEADWDNVSAKLIKHFADLLGYNITTL
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA