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The map label for this gene is pdxT [H]

Identifier: 148827073

GI number: 148827073

Start: 340251

End: 340829

Strand: Direct

Name: pdxT [H]

Synonym: CGSHiGG_01920

Alternate gene names: 148827073

Gene position: 340251-340829 (Clockwise)

Preceding gene: 148827072

Following gene: 148827075

Centisome position: 18.03

GC content: 36.27

Gene sequence:

>579_bases
ATGAAAATCGGAATATTAGCTCTACAAGGTGCCTTTGCGGAACATGCACAAATGCTAGAAAAATTAGGAATTGAAAGTGT
CGAACTGAGAAATTTAAAAAATTTTCAACAACATTACAGTGATTTATCAGGTTTGATTCTACCTGGCGGTGAGTCCACCG
CCATAGGAAAACTTTTAAGAGAGCTGTATATGCTGGAACCGATAAAACAAGCTATCTCTTCTGGCTTTCCTGTCTTTGGA
ACTTGTGCTGGTTTGATTCTGTTGGCTAAAGAGATTACTTCTCAGAAAGAGAGTCATTTTGGAACAATGGACATTGTGGT
TGAGAGGAATGCCTATGGACGCCAATTGGGAAGTTTCTATACAGAAGCAGATTGCAAAGGGGTTGGTAAAATTCCTATGA
CTTTTATCAGAGGACCTATTATCAGTAGTGTTGGTAAAAAAGTCAATATTCTTGCAACGGTAAATAATAAAATCGTTGCA
GCCCAAGAAAAGAATATGCTGGTAACATCATTTCATCCTGAATTAACAAATAACTTGAGTTTGCATAAGTACTTTATTGA
TATATGTAAAGTAGCATAA

Upstream 100 bases:

>100_bases
TCCACAAATCCTAGCAAAAATCTCCGAAGATTTAGGTGAAGCCATGGTTGGTATCAATGAAAATGAAATCCAAATTCTCA
TGGCTGAGCGAGGAAAATAA

Downstream 100 bases:

>100_bases
ACCATTATCTGCATATCTGCCCTCCCTAGGAAACAAACCATATTGTAGCAATGAGAGCAGATAATTTTCATCAAAAAACC
GAACAGTTACTAGACTTAAA

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MKIGILALQGAFAEHAQMLEKLGIESVELRNLKNFQQHYSDLSGLILPGGESTAIGKLLRELYMLEPIKQAISSGFPVFG
TCAGLILLAKEITSQKESHFGTMDIVVERNAYGRQLGSFYTEADCKGVGKIPMTFIRGPIISSVGKKVNILATVNNKIVA
AQEKNMLVTSFHPELTNNLSLHKYFIDICKVA

Sequences:

>Translated_192_residues
MKIGILALQGAFAEHAQMLEKLGIESVELRNLKNFQQHYSDLSGLILPGGESTAIGKLLRELYMLEPIKQAISSGFPVFG
TCAGLILLAKEITSQKESHFGTMDIVVERNAYGRQLGSFYTEADCKGVGKIPMTFIRGPIISSVGKKVNILATVNNKIVA
AQEKNMLVTSFHPELTNNLSLHKYFIDICKVA
>Mature_192_residues
MKIGILALQGAFAEHAQMLEKLGIESVELRNLKNFQQHYSDLSGLILPGGESTAIGKLLRELYMLEPIKQAISSGFPVFG
TCAGLILLAKEITSQKESHFGTMDIVVERNAYGRQLGSFYTEADCKGVGKIPMTFIRGPIISSVGKKVNILATVNNKIVA
AQEKNMLVTSFHPELTNNLSLHKYFIDICKVA

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS [H]

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=204, Percent_Identity=33.8235294117647, Blast_Score=108, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323995, Length=208, Percent_Identity=33.1730769230769, Blast_Score=102, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321048, Length=208, Percent_Identity=33.6538461538462, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002161
- InterPro:   IPR021196 [H]

Pfam domain/function: PF01174 SNO [H]

EC number: NA

Molecular weight: Translated: 21042; Mature: 21042

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS01236 PDXT_SNO_1 ; PS51130 PDXT_SNO_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGILALQGAFAEHAQMLEKLGIESVELRNLKNFQQHYSDLSGLILPGGESTAIGKLLR
CCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
ELYMLEPIKQAISSGFPVFGTCAGLILLAKEITSQKESHFGTMDIVVERNAYGRQLGSFY
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
TEADCKGVGKIPMTFIRGPIISSVGKKVNILATVNNKIVAAQEKNMLVTSFHPELTNNLS
HCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCEEEEECCCCEEEEECCCHHHCCCH
LHKYFIDICKVA
HHHHHHHHHHCC
>Mature Secondary Structure
MKIGILALQGAFAEHAQMLEKLGIESVELRNLKNFQQHYSDLSGLILPGGESTAIGKLLR
CCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
ELYMLEPIKQAISSGFPVFGTCAGLILLAKEITSQKESHFGTMDIVVERNAYGRQLGSFY
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
TEADCKGVGKIPMTFIRGPIISSVGKKVNILATVNNKIVAAQEKNMLVTSFHPELTNNLS
HCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCEEEEECCCCEEEEECCCHHHCCCH
LHKYFIDICKVA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA