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The map label for this gene is pyrF [H]

Identifier: 148827066

GI number: 148827066

Start: 333801

End: 334475

Strand: Reverse

Name: pyrF [H]

Synonym: CGSHiGG_01885

Alternate gene names: 148827066

Gene position: 334475-333801 (Counterclockwise)

Preceding gene: 148827067

Following gene: 148827065

Centisome position: 17.72

GC content: 40.59

Gene sequence:

>675_bases
GTGGCATTGGATTTCGAGAAAGAAGCAGAGGCTCTTGCTTTAGTTGATCAAATTGATCCAAGTTTGTGTCGATTAAAAGT
GGGTAAAGAAATGTTTACTACACTTGGTATTAATTTTGTAAAGCAATTACATCAACGTAATTTTGATGTTTTTTTGGATC
TGAAATATCACGATATTCCAAATACGGTAGCAAGAGCAGTTCGTTCTGCTGCTGATTTAGGCGTATGGATGGTGGATTTA
CACGCAAGTGGTGGCTTGCGTATGATGGAAGACGCGAAGAAAATTCTTGAACCTTATGGGAAAGATGCGCCATTGCTTAT
TGCTGTGACGGTATTAACCAGTATGGAAGACTTGGATTTATTACAAATTGGGATTAATGCATCGCCAATGGAACAAGTTT
TACGCTTAGCTCATTTAACCCAACGGGCGGGTTTAGATGGTGTAGTCTGTTCCCCTCAAGAAGTTGAAATTTTACGTAAC
GCTTGTGGAGAAGACTTTAAATTAGTGACCCCAGGGATTCGTCCAATAGGAACTGATTTTGGCGATCAACGACGTGTGAT
GACGCCTACTGCAGCAATTCGTGCAGGTTCAGATTATTTAGTTATTGGACGCCCAATAACTCAAGCGGATAATCCTGCGG
AGGTTCTCCGTTCTATTAATGTATCTATTGGGTAA

Upstream 100 bases:

>100_bases
CCCATCTTGCCGTCAATGGGAAAGTATTAAACCGGTTTCAAATCAAGAACATAATTAATTGACACTCAGTGAGGTAACAT
TATGACAAGTAAAATTAATT

Downstream 100 bases:

>100_bases
TCATAAATGTCAGATTCTGTTTTAGTGTATTCTACAGATGTAGGTCGAATTAAAGAGGAAAAATTGACTGTAGCTCGCCC
AAAAGGAGACGGTGTTGTAC

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MALDFEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDL
HASGGLRMMEDAKKILEPYGKDAPLLIAVTVLTSMEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQEVEILRN
ACGEDFKLVTPGIRPIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG

Sequences:

>Translated_224_residues
MALDFEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDL
HASGGLRMMEDAKKILEPYGKDAPLLIAVTVLTSMEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQEVEILRN
ACGEDFKLVTPGIRPIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG
>Mature_223_residues
ALDFEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDLH
ASGGLRMMEDAKKILEPYGKDAPLLIAVTVLTSMEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQEVEILRNA
CGEDFKLVTPGIRPIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=223, Percent_Identity=62.780269058296, Blast_Score=286, Evalue=9e-79,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 24551; Mature: 24420

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDFEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDLKYHDIP
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
NTVARAVRSAADLGVWMVDLHASGGLRMMEDAKKILEPYGKDAPLLIAVTVLTSMEDLDL
HHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCE
LQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQEVEILRNACGEDFKLVTPGIRPIGTDF
EEECCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCEEECCCCCCCCCCC
GDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG
CCCCEEECCHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
ALDFEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDLKYHDIP
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
NTVARAVRSAADLGVWMVDLHASGGLRMMEDAKKILEPYGKDAPLLIAVTVLTSMEDLDL
HHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCE
LQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQEVEILRNACGEDFKLVTPGIRPIGTDF
EEECCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCEEECCCCCCCCCCC
GDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG
CCCCEEECCHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]