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The map label for this gene is bcr [H]

Identifier: 148827049

GI number: 148827049

Start: 311870

End: 313066

Strand: Direct

Name: bcr [H]

Synonym: CGSHiGG_01800

Alternate gene names: 148827049

Gene position: 311870-313066 (Clockwise)

Preceding gene: 148827048

Following gene: 148827051

Centisome position: 16.53

GC content: 41.35

Gene sequence:

>1197_bases
ATGAATCAACAAAAATCGACTTTCATCTTTATTCTTACCCTCGGTATTCTCTCTATGCTACCGCCCTTTGGCGTGGATAT
GTATTTGCCGTCTTTTTTAGAAATTGCAAAGGATCTCGACGTAAGTCCAGAACAAGTACAACACACGCTCACTTCTTTTG
CTTATGGCATGGCGTTTGGTCAGCTTTTTTGGGGGCCTTTTGGTGATAGTTTCGGACGAAAACCGATCATTTTACTCGGT
GTTATTGTGGGCGCACTAACCGCTTTAGTGCTTACCGAAATAAATTCAGTTGGAAATTTTACCGCACTTCGTTTTGTGCA
AGGTTTCTTTGGTGCCGCACCTGTAGTACTTTCTGGCGCATTATTACGTGATTTATTTAGTAAAGATCAGCTATCCAAAG
TGATGTCCACCATCACGTTAGTATTTATGCTTGCACCTTTAGTTGCACCAATCATTGGTGGTTACATCGTTAAATTTTTC
CATTGGCATGCGATTTTTTACGTTATTTCGCTCGTGGGATTATTAGCCGCTGCATTGGTCTTTTTCATCATTCCAGAAAC
CCATAAAAAAGAAAATCGCATTCCGCTACGCTTAAATATTATCGCTCGCAATTTCCTTCTGCTTTGGAAGCAAAAAGAAG
TGCTTGGCTATATGTTTGCCGCCTCTTTTAGCTTTGGCGGGTTGTTTGCTTTTGTTACTGCAGGATCAATTGTCTATATC
GGCATTTATGGCGTTCCCGTCGATCAGTTCGGTTATTTCTTTATGATGAATATCGTTACGATGATCTTTGCCTCTTTTTT
AAATAGCCGATTTGTTACTAAAGTGGGTGCAGAAACAATGTTACGGATAGCCCTCGCAATTCAATTCCTTTCTGGAATGT
GGCTAATTTTGACCGCACTTTTAGATCTTGGTTTTTGGCCAATGGCAATTGGCGTCGCCTTTTTCGTCGGTCCAAACCCT
GTGATTTCATCAAATGCAATGGCATCAGCTTTAGAAAAATGTCCTCAAATGGCAGGAACAGCAAATTCTTTGATTGGTAG
CGTACGTTTTGCAGTGGGTGCAATTATGGGAAGTTTAGTCGCATCAATGAAAATGGATACTGCCGCACCAATGCTCTTTA
CTATGGGGGCATGTGTCGTGATTTCCGTGTTGGCGTATTACTTTCTAACCAGCCGAAATCTTAAAAGCCGTGGGTAA

Upstream 100 bases:

>100_bases
CATCGCTGGAAAATTGGTGATGTAGTGTTGGATGAATCCTTAGAAGAAGGCGAATACCGCCCATTAACTCAAAGTGAAAT
TGAAAAGTTAGTAAAATAAT

Downstream 100 bases:

>100_bases
ATTCCCCACGGTGCAATGATTGTTTTCTTTATTAAACAAAAACCATCCATAACGGCAAGCAAACAGCAATAAATAATAGG
ATAAATAAACTATTGGTAGA

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNQQKSTFIFILTLGILSMLPPFGVDMYLPSFLEIAKDLDVSPEQVQHTLTSFAYGMAFGQLFWGPFGDSFGRKPIILLG
VIVGALTALVLTEINSVGNFTALRFVQGFFGAAPVVLSGALLRDLFSKDQLSKVMSTITLVFMLAPLVAPIIGGYIVKFF
HWHAIFYVISLVGLLAAALVFFIIPETHKKENRIPLRLNIIARNFLLLWKQKEVLGYMFAASFSFGGLFAFVTAGSIVYI
GIYGVPVDQFGYFFMMNIVTMIFASFLNSRFVTKVGAETMLRIALAIQFLSGMWLILTALLDLGFWPMAIGVAFFVGPNP
VISSNAMASALEKCPQMAGTANSLIGSVRFAVGAIMGSLVASMKMDTAAPMLFTMGACVVISVLAYYFLTSRNLKSRG

Sequences:

>Translated_398_residues
MNQQKSTFIFILTLGILSMLPPFGVDMYLPSFLEIAKDLDVSPEQVQHTLTSFAYGMAFGQLFWGPFGDSFGRKPIILLG
VIVGALTALVLTEINSVGNFTALRFVQGFFGAAPVVLSGALLRDLFSKDQLSKVMSTITLVFMLAPLVAPIIGGYIVKFF
HWHAIFYVISLVGLLAAALVFFIIPETHKKENRIPLRLNIIARNFLLLWKQKEVLGYMFAASFSFGGLFAFVTAGSIVYI
GIYGVPVDQFGYFFMMNIVTMIFASFLNSRFVTKVGAETMLRIALAIQFLSGMWLILTALLDLGFWPMAIGVAFFVGPNP
VISSNAMASALEKCPQMAGTANSLIGSVRFAVGAIMGSLVASMKMDTAAPMLFTMGACVVISVLAYYFLTSRNLKSRG
>Mature_398_residues
MNQQKSTFIFILTLGILSMLPPFGVDMYLPSFLEIAKDLDVSPEQVQHTLTSFAYGMAFGQLFWGPFGDSFGRKPIILLG
VIVGALTALVLTEINSVGNFTALRFVQGFFGAAPVVLSGALLRDLFSKDQLSKVMSTITLVFMLAPLVAPIIGGYIVKFF
HWHAIFYVISLVGLLAAALVFFIIPETHKKENRIPLRLNIIARNFLLLWKQKEVLGYMFAASFSFGGLFAFVTAGSIVYI
GIYGVPVDQFGYFFMMNIVTMIFASFLNSRFVTKVGAETMLRIALAIQFLSGMWLILTALLDLGFWPMAIGVAFFVGPNP
VISSNAMASALEKCPQMAGTANSLIGSVRFAVGAIMGSLVASMKMDTAAPMLFTMGACVVISVLAYYFLTSRNLKSRG

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=383, Percent_Identity=42.8198433420365, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI48994889, Length=183, Percent_Identity=32.2404371584699, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1790146, Length=172, Percent_Identity=31.3953488372093, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI87082312, Length=349, Percent_Identity=25.214899713467, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790794, Length=309, Percent_Identity=23.3009708737864, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787065, Length=386, Percent_Identity=24.6113989637306, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321838, Length=162, Percent_Identity=29.0123456790123, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43432; Mature: 43432

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]