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The map label for this gene is 148827016

Identifier: 148827016

GI number: 148827016

Start: 258986

End: 260365

Strand: Direct

Name: 148827016

Synonym: CGSHiGG_01530

Alternate gene names: NA

Gene position: 258986-260365 (Clockwise)

Preceding gene: 148827015

Following gene: 148827017

Centisome position: 13.72

GC content: 36.3

Gene sequence:

>1380_bases
ATGAGTAAACTTGGTAATTATATCTCGGTACAAAATGGATATGCTTTCAAAAGCAAAGATTTTATAAAGAATTTATCTGG
AATGCCTGTAATTAAAATTGGTAATGTAACTGGTGGCAGTTTTATTGATTTGAGTAGCTATGACACTATATCAGAAGAAA
TTGCAAGAAAAGTAAAATCTTTTCAGACAAAAGATGACGATATTTTAATTGCAATGACAGGAGCAAATGTGGGGAAAGTC
TCTCGAATTGCAAAAGGAACTCAACCCTGTCTGATTAATCAACGTGTTGGTCGTCTTATATTAAAAGAAGATTGCCCATA
CTCATCTGATTTTATTTATTATTTAGTAAGTTCAAATAAAAGTTTCCAATATTTTTCTAATACAGCTGATGGCGCAGCAC
AACCTAATATCAGCGGAAAACTTATTGAAGATCTAGAATTTCCAGATATTTCGCCCAAATCAGCTAATAAAGCAGGAAAA
CACTTAAAGGTACTGGATGAAAAAATCCAACTCAACACCCAAATCAACCAAACCTTGGAACAAATCGCTCAAGCCCTGTT
TAAAAGCTGGTTTGTCGATTTCGATCCCGTGCGCGCCAAAGTCCAAGCCCTTTCAGAGGGCATGAGCCTTGAACAAGCGG
AACTGGCAGCCATGCAGACAATCAGCGGAAAAACACCCGAAGAACTGACCGCACTTTCACAAACACAACCTGACCGCTAC
GCCGAATTAGCCGAAACCGCCAAAGCGTTTCCGTGTGAGATGGTGGAGGTTGATGGGGGCGAGGTGCCGAAGGGGTGGGA
AAAAACAACACTTTCAGAAATTTGTGAAATGCAAAATGGTTATGCATTCAAAAGTTTTGACTGGATGGAGCAAGGTATAC
CTGTTATTAAAATTGGATCTGTTAAGCCAATAATTGTAGAAGTAGAAGGAAATGGTTTTGTTTCTGAAGACTACTCAAAA
TTAAAACCTGATTTCTTGCTTACAAGCAGCGATATTTTAGTTGGCTTAACAGGTTATGTTGGTGAAGTTGGACGCATTCC
AACAGGAAAAATTGCAATGCTAAATCAACGAGTAGCAAAATTCTTACCAAAAGAGATAGATAAGAATCATTGTTTCTATA
ATTACATTTATTGCTTAGCACGACAATCCCAATTTAAAGAGTTTGCAGAAATAAATGCTAAAGGATCCGCACAGGCAAAT
ATCAGCACAAAAGAACTACTCAAATTCCCGATTATCAAAGCAAATGATAAATTACATATTTTGTTTGAAAATAGAGTAAA
AGAACTTTTAGAAAGAATTTTATGGAATTCTCAAAACGCTGAAACTTTAGCTAAGACTAGAGATTTATTATTACCTAGAT
TGTTAAATGGGGAAGTTTAA

Upstream 100 bases:

>100_bases
GCAGAAAAAATGCAAAATCTGACCGCTCTTTTGAAGGAACAGTTTGCAAAAAGTGCAGAGTTGGAAGCAGAGATTAAGAA
GAATTTAGGGGGATTGGGGT

Downstream 100 bases:

>100_bases
AATGGAAGATTTTAAATTTATAGGAAAAGATAAAGTCCCAGTTGTTGGTGAAATCAATACGTTAGATGAATTCAAAAGAT
TTGTTTTTAGTTATAGTTAT

Product: putative type I restriction enzyme HindVIIP specificity protein

Products: NA

Alternate protein names: S.HindVIIP; Type I restriction enzyme HindVIIP specificity protein; S protein [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSKLGNYISVQNGYAFKSKDFIKNLSGMPVIKIGNVTGGSFIDLSSYDTISEEIARKVKSFQTKDDDILIAMTGANVGKV
SRIAKGTQPCLINQRVGRLILKEDCPYSSDFIYYLVSSNKSFQYFSNTADGAAQPNISGKLIEDLEFPDISPKSANKAGK
HLKVLDEKIQLNTQINQTLEQIAQALFKSWFVDFDPVRAKVQALSEGMSLEQAELAAMQTISGKTPEELTALSQTQPDRY
AELAETAKAFPCEMVEVDGGEVPKGWEKTTLSEICEMQNGYAFKSFDWMEQGIPVIKIGSVKPIIVEVEGNGFVSEDYSK
LKPDFLLTSSDILVGLTGYVGEVGRIPTGKIAMLNQRVAKFLPKEIDKNHCFYNYIYCLARQSQFKEFAEINAKGSAQAN
ISTKELLKFPIIKANDKLHILFENRVKELLERILWNSQNAETLAKTRDLLLPRLLNGEV

Sequences:

>Translated_459_residues
MSKLGNYISVQNGYAFKSKDFIKNLSGMPVIKIGNVTGGSFIDLSSYDTISEEIARKVKSFQTKDDDILIAMTGANVGKV
SRIAKGTQPCLINQRVGRLILKEDCPYSSDFIYYLVSSNKSFQYFSNTADGAAQPNISGKLIEDLEFPDISPKSANKAGK
HLKVLDEKIQLNTQINQTLEQIAQALFKSWFVDFDPVRAKVQALSEGMSLEQAELAAMQTISGKTPEELTALSQTQPDRY
AELAETAKAFPCEMVEVDGGEVPKGWEKTTLSEICEMQNGYAFKSFDWMEQGIPVIKIGSVKPIIVEVEGNGFVSEDYSK
LKPDFLLTSSDILVGLTGYVGEVGRIPTGKIAMLNQRVAKFLPKEIDKNHCFYNYIYCLARQSQFKEFAEINAKGSAQAN
ISTKELLKFPIIKANDKLHILFENRVKELLERILWNSQNAETLAKTRDLLLPRLLNGEV
>Mature_458_residues
SKLGNYISVQNGYAFKSKDFIKNLSGMPVIKIGNVTGGSFIDLSSYDTISEEIARKVKSFQTKDDDILIAMTGANVGKVS
RIAKGTQPCLINQRVGRLILKEDCPYSSDFIYYLVSSNKSFQYFSNTADGAAQPNISGKLIEDLEFPDISPKSANKAGKH
LKVLDEKIQLNTQINQTLEQIAQALFKSWFVDFDPVRAKVQALSEGMSLEQAELAAMQTISGKTPEELTALSQTQPDRYA
ELAETAKAFPCEMVEVDGGEVPKGWEKTTLSEICEMQNGYAFKSFDWMEQGIPVIKIGSVKPIIVEVEGNGFVSEDYSKL
KPDFLLTSSDILVGLTGYVGEVGRIPTGKIAMLNQRVAKFLPKEIDKNHCFYNYIYCLARQSQFKEFAEINAKGSAQANI
STKELLKFPIIKANDKLHILFENRVKELLERILWNSQNAETLAKTRDLLLPRLLNGEV

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. Subunit S dictates DNA sequences specificity [H]

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 51111; Mature: 50980

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLGNYISVQNGYAFKSKDFIKNLSGMPVIKIGNVTGGSFIDLSSYDTISEEIARKVKS
CCCCCCEEEECCCCCCCCHHHHHCCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHH
FQTKDDDILIAMTGANVGKVSRIAKGTQPCLINQRVGRLILKEDCPYSSDFIYYLVSSNK
CCCCCCCEEEEEECCCCHHHHHHHCCCCCEEEHHHHCCEEEECCCCCCCCEEEEEEECCC
SFQYFSNTADGAAQPNISGKLIEDLEFPDISPKSANKAGKHLKVLDEKIQLNTQINQTLE
CEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHH
QIAQALFKSWFVDFDPVRAKVQALSEGMSLEQAELAAMQTISGKTPEELTALSQTQPDRY
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHH
AELAETAKAFPCEMVEVDGGEVPKGWEKTTLSEICEMQNGYAFKSFDWMEQGIPVIKIGS
HHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCEECCHHHHHCCCCEEEECC
VKPIIVEVEGNGFVSEDYSKLKPDFLLTSSDILVGLTGYVGEVGRIPTGKIAMLNQRVAK
CCEEEEEECCCCCCCCCHHHCCCCEEEECCCCEEEEHHHHCCCCCCCCCHHHHHHHHHHH
FLPKEIDKNHCFYNYIYCLARQSQFKEFAEINAKGSAQANISTKELLKFPIIKANDKLHI
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCEECCCCEEE
LFENRVKELLERILWNSQNAETLAKTRDLLLPRLLNGEV
EEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKLGNYISVQNGYAFKSKDFIKNLSGMPVIKIGNVTGGSFIDLSSYDTISEEIARKVKS
CCCCCEEEECCCCCCCCHHHHHCCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHH
FQTKDDDILIAMTGANVGKVSRIAKGTQPCLINQRVGRLILKEDCPYSSDFIYYLVSSNK
CCCCCCCEEEEEECCCCHHHHHHHCCCCCEEEHHHHCCEEEECCCCCCCCEEEEEEECCC
SFQYFSNTADGAAQPNISGKLIEDLEFPDISPKSANKAGKHLKVLDEKIQLNTQINQTLE
CEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHH
QIAQALFKSWFVDFDPVRAKVQALSEGMSLEQAELAAMQTISGKTPEELTALSQTQPDRY
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHH
AELAETAKAFPCEMVEVDGGEVPKGWEKTTLSEICEMQNGYAFKSFDWMEQGIPVIKIGS
HHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCEECCHHHHHCCCCEEEECC
VKPIIVEVEGNGFVSEDYSKLKPDFLLTSSDILVGLTGYVGEVGRIPTGKIAMLNQRVAK
CCEEEEEECCCCCCCCCHHHCCCCEEEECCCCEEEEHHHHCCCCCCCCCHHHHHHHHHHH
FLPKEIDKNHCFYNYIYCLARQSQFKEFAEINAKGSAQANISTKELLKFPIIKANDKLHI
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCEECCCCEEE
LFENRVKELLERILWNSQNAETLAKTRDLLLPRLLNGEV
EEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]