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The map label for this gene is thiI [H]

Identifier: 148826939

GI number: 148826939

Start: 182550

End: 184007

Strand: Reverse

Name: thiI [H]

Synonym: CGSHiGG_01065

Alternate gene names: 148826939

Gene position: 184007-182550 (Counterclockwise)

Preceding gene: 148826943

Following gene: 148826938

Centisome position: 9.75

GC content: 37.17

Gene sequence:

>1458_bases
ATGAAATTTATCGTTAAACTTTTTCCTGAAATTATGATTAAGAGCGAAACTGTGCGTAAACGTTTCGCAAAAATTTTAAC
CTCTAATATCCGCAATATTTTACAGAAATATGATGAAGAAACTGCAGTTGTGCGTCACTGGGATTACATTGAAGTGCGGT
CAAAAAATGAAGAGAATCGTGAAGAATTAATCGCCTTGTTACAACGTATTCCTGGTATTCATCATTTTTTAGAAGTTGAA
GAAAAACCGTTTACCGATTTACATCATATTTTTGAATTGACGCTTGCGGATGTCGCACAACAACTTCAAGGCAAAACCTT
TTGCGTTCGAGTAAAACGTAAGGGTAAACATAAATTTAGTTCGATTGAAGCAGAACGTTATATCGGCGGTGGATTAAATC
AACATATTGAAAGCACAAAAGTGCGGTTAAAAAATCCAGATGTGACTGTTCGTATTGATATTGAAGATGACAAAATGATG
TTAGTTAAAGCACGTCACGCTGGTATTGGTGGTTATCCGATTGGTACGCAAGAGGATGTGCTTTCTTTAATTTCGGGGGG
ATTTGATTCAGGCGTATCGAGTTATATGTTGATTCGTCGTGGTTCCCGAGTTCATTATTGTTTCTTTAATCTTGGCGGTG
CAGCCCATGAAATTGGCGTTAAACAAATGGCTTATCATATTTGGCAACGCTATAGTGCTTCACATAAAGTACGCTTTATT
GCGATTAACTTTGAGGGAGTTGTCGGTGAGATTTTAGAGAAAGTCGATAACGGACAAATGGGCGTTGTATTAAAACGGAT
GATGGTACGTGCAGCAAGTAAAGTCGCTCAACGTTTTAATATTGAAGCGATTGTGACAGGGGAGGCATTAGGGCAAGTTT
CTAGCCAAACTTTAACCAATTTACGCTTGATTGATGAAGCCGCTGATGCCTTAGTATTACGTCCGTTAATTACCCATGAT
AAAGAACAAATTATCGCGATGGCGAAAGAAATTGGTACTGACGATATTGCAAAATCTATGCCTGAATTTTGTGGCGTGAT
TTCAAAAAATCCTACGATCAAAGCTGTGCGTGAAAAGATTCTTGCAGAAGAAGGGAATTTTAATTTTGAGATTCTTGAAA
GTGCGGTACAAAATGCGAAATATTTAGATATTCGCCAGATCGCAGAAGAAACAGAAAAAGAAGTCGTAGAAGTCGAGGCA
ATTTCTGTATTGGGTGAAAATGAAGTGATTTTGGATATTCGTAGCCCAGAAGAAACGGATGAAAAGCCATTTGAATCAGG
TGCACATGACGTCATTCAAATGCCGTTCTACAAACTTTCTTCTCAATTTGGTAGCCTTGATCAAAGTAAAAATTACGTGT
TGTATTGTGAACGTGGTGTGATGAGTAAATTACAAGCCTTATATTTGAAAGAAAATGGTTTTTCAAATGTGCGTGTATTT
GCAAAAAATATTCATTAA

Upstream 100 bases:

>100_bases
TGTTAAAAAATTATTTCAAGCTAGGACCAGTGGACTTTTCACTATCTTTGCGTAAAATACTCGCCACTTTAAATACCCAA
GAAATACCTAAGCTATTTAT

Downstream 100 bases:

>100_bases
TTAGGATATAAAATATGCGTAATCAAACTAAACAGCATCTTGAACAGCTTCAAATTACCATGCAGCAACTTAATTTATGG
CAAACGATGCCCCCTGCTGC

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKFIVKLFPEIMIKSETVRKRFAKILTSNIRNILQKYDEETAVVRHWDYIEVRSKNEENREELIALLQRIPGIHHFLEVE
EKPFTDLHHIFELTLADVAQQLQGKTFCVRVKRKGKHKFSSIEAERYIGGGLNQHIESTKVRLKNPDVTVRIDIEDDKMM
LVKARHAGIGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWQRYSASHKVRFI
AINFEGVVGEILEKVDNGQMGVVLKRMMVRAASKVAQRFNIEAIVTGEALGQVSSQTLTNLRLIDEAADALVLRPLITHD
KEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTIKAVREKILAEEGNFNFEILESAVQNAKYLDIRQIAEETEKEVVEVEA
ISVLGENEVILDIRSPEETDEKPFESGAHDVIQMPFYKLSSQFGSLDQSKNYVLYCERGVMSKLQALYLKENGFSNVRVF
AKNIH

Sequences:

>Translated_485_residues
MKFIVKLFPEIMIKSETVRKRFAKILTSNIRNILQKYDEETAVVRHWDYIEVRSKNEENREELIALLQRIPGIHHFLEVE
EKPFTDLHHIFELTLADVAQQLQGKTFCVRVKRKGKHKFSSIEAERYIGGGLNQHIESTKVRLKNPDVTVRIDIEDDKMM
LVKARHAGIGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWQRYSASHKVRFI
AINFEGVVGEILEKVDNGQMGVVLKRMMVRAASKVAQRFNIEAIVTGEALGQVSSQTLTNLRLIDEAADALVLRPLITHD
KEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTIKAVREKILAEEGNFNFEILESAVQNAKYLDIRQIAEETEKEVVEVEA
ISVLGENEVILDIRSPEETDEKPFESGAHDVIQMPFYKLSSQFGSLDQSKNYVLYCERGVMSKLQALYLKENGFSNVRVF
AKNIH
>Mature_485_residues
MKFIVKLFPEIMIKSETVRKRFAKILTSNIRNILQKYDEETAVVRHWDYIEVRSKNEENREELIALLQRIPGIHHFLEVE
EKPFTDLHHIFELTLADVAQQLQGKTFCVRVKRKGKHKFSSIEAERYIGGGLNQHIESTKVRLKNPDVTVRIDIEDDKMM
LVKARHAGIGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWQRYSASHKVRFI
AINFEGVVGEILEKVDNGQMGVVLKRMMVRAASKVAQRFNIEAIVTGEALGQVSSQTLTNLRLIDEAADALVLRPLITHD
KEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTIKAVREKILAEEGNFNFEILESAVQNAKYLDIRQIAEETEKEVVEVEA
ISVLGENEVILDIRSPEETDEKPFESGAHDVIQMPFYKLSSQFGSLDQSKNYVLYCERGVMSKLQALYLKENGFSNVRVF
AKNIH

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=480, Percent_Identity=67.9166666666667, Blast_Score=691, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 55013; Mature: 55013

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS51165 THUMP ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIVKLFPEIMIKSETVRKRFAKILTSNIRNILQKYDEETAVVRHWDYIEVRSKNEENR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCEEEEECCCCCCH
EELIALLQRIPGIHHFLEVEEKPFTDLHHIFELTLADVAQQLQGKTFCVRVKRKGKHKFS
HHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHH
SIEAERYIGGGLNQHIESTKVRLKNPDVTVRIDIEDDKMMLVKARHAGIGGYPIGTQEDV
HHHHHHHHCCCHHHHHHHCEEEEECCCEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWQRYSASHKVRFI
HHHHHCCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
AINFEGVVGEILEKVDNGQMGVVLKRMMVRAASKVAQRFNIEAIVTGEALGQVSSQTLTN
EEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHH
LRLIDEAADALVLRPLITHDKEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTIKAVREKI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAEEGNFNFEILESAVQNAKYLDIRQIAEETEKEVVEVEAISVLGENEVILDIRSPEETD
HHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCC
EKPFESGAHDVIQMPFYKLSSQFGSLDQSKNYVLYCERGVMSKLQALYLKENGFSNVRVF
CCCHHCCCHHHHHCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEE
AKNIH
EECCC
>Mature Secondary Structure
MKFIVKLFPEIMIKSETVRKRFAKILTSNIRNILQKYDEETAVVRHWDYIEVRSKNEENR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCEEEEECCCCCCH
EELIALLQRIPGIHHFLEVEEKPFTDLHHIFELTLADVAQQLQGKTFCVRVKRKGKHKFS
HHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHH
SIEAERYIGGGLNQHIESTKVRLKNPDVTVRIDIEDDKMMLVKARHAGIGGYPIGTQEDV
HHHHHHHHCCCHHHHHHHCEEEEECCCEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWQRYSASHKVRFI
HHHHHCCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
AINFEGVVGEILEKVDNGQMGVVLKRMMVRAASKVAQRFNIEAIVTGEALGQVSSQTLTN
EEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHH
LRLIDEAADALVLRPLITHDKEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTIKAVREKI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAEEGNFNFEILESAVQNAKYLDIRQIAEETEKEVVEVEAISVLGENEVILDIRSPEETD
HHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCC
EKPFESGAHDVIQMPFYKLSSQFGSLDQSKNYVLYCERGVMSKLQALYLKENGFSNVRVF
CCCHHCCCHHHHHCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEE
AKNIH
EECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA