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The map label for this gene is topA [H]

Identifier: 148826835

GI number: 148826835

Start: 90528

End: 93134

Strand: Direct

Name: topA [H]

Synonym: CGSHiGG_00485

Alternate gene names: 148826835

Gene position: 90528-93134 (Clockwise)

Preceding gene: 148826834

Following gene: 148826837

Centisome position: 4.8

GC content: 39.09

Gene sequence:

>2607_bases
ATGAGTAAATCCTTGGTGATTGTGGAGTCGCCAGCAAAAGCAAAAACTATTAATAAGTATTTAGGTAGCCAATATGTCGT
GAAATCGAGCGTTGGACATATTCGTGATTTGCCGACGGTTGGTTCTAGTACAGGTGAAAAAGCTAAACCAATTTCAACCA
AAGGTATGGATGCCGAAGAAAAAGCAAAAATTAAGGCAGAAAAGGAACGTAGCGCCTTAGTTAAACGTATGGGGATTGAT
CCTTATCACGATTGGAAAGCAAATTATCAGATTTTGCCTGGTAAAGAAAAAGTGGTTTCCGAGTTAAAGTCGCTAGCTAA
AAAAGCAGATCACATTTATCTCGCAACCGACTTGGATAGAGAAGGGGAAGCGATTGCGTGGCATTTACGTGAAGTGATTG
GTGGTAACGATGATCGTTTTAGTCGCGTGGTGTTTAACGAAATAACTAAAAATGCCATTAAACAGGCTTTTGAAAAGCCT
GAACAATTAAATATGGATCGTGTTAATGCTCAACAAACTCGTCGTTTTTTAGATAGAGTAGTGGGCTTTATGGTGTCGCC
ATTACTTTGGAAAAAAGTGGCACGCGGGCTTTCTGCTGGTCGAGTTCAATCGGTTGCGGTTAAATTATTAGTAGAACGCG
AGCGTGAAATTAAAGCGTTCCAGCCTGAAGAATATTGGGAAGTTGCCGTCCTTACGAATAATCAAAATAAACAAGCTATA
CGCTTGGATGTTACGGATTATAAAGGCAAGAAATTTGATCCAAAAAATCAAAAAGAAGCACAAAGTGCGGTAGATTTTCT
GAATGTTTCAGATTACGTTGTCACAGATTTGGAAACTAAGCCAACAAGTTCTCGCCCTCGAGCACCATTTATTACATCAA
CGCTTCAACAAACTGCAAGTACTCGCTTGGGATTTGGCGTTAAAAAAACAATGATGTTAGCCCAACGTTTATATGAAGCA
GGTTACATTACTTATATGCGTACGGATTCAACCAATTTGAGTCAAGATGCGCTTAATATGGCGCGTAGCTATATTGAAAA
TCATTTTGGTGCACAATATTTACCAGAAAAACCAAATTTCTATTCTAGTAAAGAAAATGCGCAAGAGGCGCATGAGGCTA
TTCGTCCATCAGATATTCGAGCATTGCCTGAATCTTTAGAGGGAATGGAAAAAGATGCGGTACGCCTTTATGATTTAATT
TGGCGTCAATTCTTAGCATGTCAAATGCCACCTGCACAATATGATAGTAGCACGCTGACCGTTACCGCTGGCGATTATAC
CTTGAAAGCAAAGGGCAGAATTTTACGTTTTGATGGTTGGACAAAAGTATTGCCACAAATAGGCAAAAATCCTGAAGATC
AAGAATTGCCTTCAGTTACTGTGTCAGAAAAACTTGCTTTAAAAGAAGTGCAACCAACACAACACTTTACCAAACCACCT
GCTCGTTTTACTGAAGCTGCTTTAGTAAAAGAATTGGAAAAACGTGGTATTGGCAGACCTTCCACTTATGCGGCAATTAT
TTCCACCATTCAAGAACGAGGTTATGTTCGTACTGAAAATCGTCGTTTCTATGCAGAAAAGATGGGCGAAATTGTGACTG
ATCGCCTCAATGAGTCTTTTGGCGAATTAATGAATTACGATTTCACCGCAAATATGGAAGATACTTTAGATAAAATTGCC
TCTGGTTCAGTAAATTGGAAAACTGAATTGAATCAATTCTTTAAGGATTTTTCTAGCCAGCTTTCTAAAGCTGAATTAGA
TGAACTTGAAGGTGGTATGCGCCCGAATAATCTCGTGGAAACCGATATTAAATGCCCAACTTGTGACAGAAAAATGGCCA
TTCGTACGGCAAGTACGGGGGTATTTTTAGGATGCTCGGGTTATGCATTGCCGCCAAAAGAGCGGTGTAAAACGACGATT
AATTTAATTCCTGAAGCGGAGTTATTAAATGTTTTAGATGAATCTTCAGAAACTAAAGCATTAATGGATCGTAAACGTTG
TACAAAATGTGGTACAGCAATGGACAGCTATGTAATCGATGCCCATCGTAAAATCCATATTTGCGGTAATAATCCAAATT
GTGATGGATATTTAATTGAAGAAGGCTCATTCAAAATTAAAGGTTATGATGGCCCTGTTGTTGAGTGTGATAAATGCGGT
GCGGATATGCATCTAAAACTTGGTCGTTTTGGCAAGTATATGGGATGTACAAATTGTGATAACACGCGCAAAATTTTGAA
AAATGGCGAGGTCGCACCACCGAAAGAAGAGCCAGTACATTTCCCTGAGTTAAAATGCGAAAAATCTGATGCATATTTTG
TATTACGTGATGGTGCGAGTGGTGTATTTATGTCAGCCCATAACTTCCCTAAATCACGCGAAACACGCCCAGTTAAAATC
GCTGAACTAGTGCAATATCGCGAGCGTTTACCTGAAAAATTAGCTTATTTAGCCGATGCTCCACAAAAAGACCCAGAAGA
GAATGAAGCGATTGTTCGTTTTAGTCGTAAAGAGAAGAAACAATATGTGACTTCTGAAAAAGAGGGAAAAGCGACTAAAT
GGATTGTTGATTTCACCAATGGAAAATGGGTTGAACGAAAGAAATAA

Upstream 100 bases:

>100_bases
TCCATTTATAGACCTTTTTTGAAAAAATGCTTGATTTTGCCAACATAACTTTGCACTATACCGCCAATTTTACAAAATTC
TAGTAATATTAGGTAATCAA

Downstream 100 bases:

>100_bases
GAAAAAAAGTGCGGTAAATTACCGCACTTTTTTGTTTGTAATACATTTTCTTATGTTTTGTAGATTATCTGAGGATTAAA
GGGCTGCTACAATTTCATTA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 868; Mature: 867

Protein sequence:

>868_residues
MSKSLVIVESPAKAKTINKYLGSQYVVKSSVGHIRDLPTVGSSTGEKAKPISTKGMDAEEKAKIKAEKERSALVKRMGID
PYHDWKANYQILPGKEKVVSELKSLAKKADHIYLATDLDREGEAIAWHLREVIGGNDDRFSRVVFNEITKNAIKQAFEKP
EQLNMDRVNAQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEVAVLTNNQNKQAI
RLDVTDYKGKKFDPKNQKEAQSAVDFLNVSDYVVTDLETKPTSSRPRAPFITSTLQQTASTRLGFGVKKTMMLAQRLYEA
GYITYMRTDSTNLSQDALNMARSYIENHFGAQYLPEKPNFYSSKENAQEAHEAIRPSDIRALPESLEGMEKDAVRLYDLI
WRQFLACQMPPAQYDSSTLTVTAGDYTLKAKGRILRFDGWTKVLPQIGKNPEDQELPSVTVSEKLALKEVQPTQHFTKPP
ARFTEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLNESFGELMNYDFTANMEDTLDKIA
SGSVNWKTELNQFFKDFSSQLSKAELDELEGGMRPNNLVETDIKCPTCDRKMAIRTASTGVFLGCSGYALPPKERCKTTI
NLIPEAELLNVLDESSETKALMDRKRCTKCGTAMDSYVIDAHRKIHICGNNPNCDGYLIEEGSFKIKGYDGPVVECDKCG
ADMHLKLGRFGKYMGCTNCDNTRKILKNGEVAPPKEEPVHFPELKCEKSDAYFVLRDGASGVFMSAHNFPKSRETRPVKI
AELVQYRERLPEKLAYLADAPQKDPEENEAIVRFSRKEKKQYVTSEKEGKATKWIVDFTNGKWVERKK

Sequences:

>Translated_868_residues
MSKSLVIVESPAKAKTINKYLGSQYVVKSSVGHIRDLPTVGSSTGEKAKPISTKGMDAEEKAKIKAEKERSALVKRMGID
PYHDWKANYQILPGKEKVVSELKSLAKKADHIYLATDLDREGEAIAWHLREVIGGNDDRFSRVVFNEITKNAIKQAFEKP
EQLNMDRVNAQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEVAVLTNNQNKQAI
RLDVTDYKGKKFDPKNQKEAQSAVDFLNVSDYVVTDLETKPTSSRPRAPFITSTLQQTASTRLGFGVKKTMMLAQRLYEA
GYITYMRTDSTNLSQDALNMARSYIENHFGAQYLPEKPNFYSSKENAQEAHEAIRPSDIRALPESLEGMEKDAVRLYDLI
WRQFLACQMPPAQYDSSTLTVTAGDYTLKAKGRILRFDGWTKVLPQIGKNPEDQELPSVTVSEKLALKEVQPTQHFTKPP
ARFTEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLNESFGELMNYDFTANMEDTLDKIA
SGSVNWKTELNQFFKDFSSQLSKAELDELEGGMRPNNLVETDIKCPTCDRKMAIRTASTGVFLGCSGYALPPKERCKTTI
NLIPEAELLNVLDESSETKALMDRKRCTKCGTAMDSYVIDAHRKIHICGNNPNCDGYLIEEGSFKIKGYDGPVVECDKCG
ADMHLKLGRFGKYMGCTNCDNTRKILKNGEVAPPKEEPVHFPELKCEKSDAYFVLRDGASGVFMSAHNFPKSRETRPVKI
AELVQYRERLPEKLAYLADAPQKDPEENEAIVRFSRKEKKQYVTSEKEGKATKWIVDFTNGKWVERKK
>Mature_867_residues
SKSLVIVESPAKAKTINKYLGSQYVVKSSVGHIRDLPTVGSSTGEKAKPISTKGMDAEEKAKIKAEKERSALVKRMGIDP
YHDWKANYQILPGKEKVVSELKSLAKKADHIYLATDLDREGEAIAWHLREVIGGNDDRFSRVVFNEITKNAIKQAFEKPE
QLNMDRVNAQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEVAVLTNNQNKQAIR
LDVTDYKGKKFDPKNQKEAQSAVDFLNVSDYVVTDLETKPTSSRPRAPFITSTLQQTASTRLGFGVKKTMMLAQRLYEAG
YITYMRTDSTNLSQDALNMARSYIENHFGAQYLPEKPNFYSSKENAQEAHEAIRPSDIRALPESLEGMEKDAVRLYDLIW
RQFLACQMPPAQYDSSTLTVTAGDYTLKAKGRILRFDGWTKVLPQIGKNPEDQELPSVTVSEKLALKEVQPTQHFTKPPA
RFTEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLNESFGELMNYDFTANMEDTLDKIAS
GSVNWKTELNQFFKDFSSQLSKAELDELEGGMRPNNLVETDIKCPTCDRKMAIRTASTGVFLGCSGYALPPKERCKTTIN
LIPEAELLNVLDESSETKALMDRKRCTKCGTAMDSYVIDAHRKIHICGNNPNCDGYLIEEGSFKIKGYDGPVVECDKCGA
DMHLKLGRFGKYMGCTNCDNTRKILKNGEVAPPKEEPVHFPELKCEKSDAYFVLRDGASGVFMSAHNFPKSRETRPVKIA
ELVQYRERLPEKLAYLADAPQKDPEENEAIVRFSRKEKKQYVTSEKEGKATKWIVDFTNGKWVERKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=570, Percent_Identity=27.1929824561404, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI4507635, Length=525, Percent_Identity=25.5238095238095, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI1787529, Length=871, Percent_Identity=71.8714121699196, Blast_Score=1320, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=541, Percent_Identity=23.4750462107209, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=593, Percent_Identity=25.9696458684654, Blast_Score=135, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=502, Percent_Identity=25.6972111553785, Blast_Score=127, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=485, Percent_Identity=26.3917525773196, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24585251, Length=624, Percent_Identity=23.8782051282051, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640096, Length=500, Percent_Identity=25.2, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 98242; Mature: 98111

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSLVIVESPAKAKTINKYLGSQYVVKSSVGHIRDLPTVGSSTGEKAKPISTKGMDAEE
CCCCEEEEECCCHHHHHHHHHCCHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCHHH
KAKIKAEKERSALVKRMGIDPYHDWKANYQILPGKEKVVSELKSLAKKADHIYLATDLDR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCEEEEEECCCC
EGEAIAWHLREVIGGNDDRFSRVVFNEITKNAIKQAFEKPEQLNMDRVNAQQTRRFLDRV
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHCCHHHHHHHHHHH
VGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEVAVLTNNQNKQAI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEE
RLDVTDYKGKKFDPKNQKEAQSAVDFLNVSDYVVTDLETKPTSSRPRAPFITSTLQQTAS
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH
TRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSQDALNMARSYIENHFGAQYLPEKPNF
HHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
YSSKENAQEAHEAIRPSDIRALPESLEGMEKDAVRLYDLIWRQFLACQMPPAQYDSSTLT
CCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VTAGDYTLKAKGRILRFDGWTKVLPQIGKNPEDQELPSVTVSEKLALKEVQPTQHFTKPP
EEECCEEEEECCCEEEECCHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHCCCHHHCCCCC
ARFTEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLNESF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHH
GELMNYDFTANMEDTLDKIASGSVNWKTELNQFFKDFSSQLSKAELDELEGGMRPNNLVE
HHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
TDIKCPTCDRKMAIRTASTGVFLGCSGYALPPKERCKTTINLIPEAELLNVLDESSETKA
CCCCCCCCCCHHEEEEECCCEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCHHHH
LMDRKRCTKCGTAMDSYVIDAHRKIHICGNNPNCDGYLIEEGSFKIKGYDGPVVECDKCG
HHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCEEEEECCCEEEECCCCCEEEHHHCC
ADMHLKLGRFGKYMGCTNCDNTRKILKNGEVAPPKEEPVHFPELKCEKSDAYFVLRDGAS
CCCEEEHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCC
GVFMSAHNFPKSRETRPVKIAELVQYRERLPEKLAYLADAPQKDPEENEAIVRFSRKEKK
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
QYVTSEKEGKATKWIVDFTNGKWVERKK
HHHCCCCCCCCEEEEEECCCCCEECCCC
>Mature Secondary Structure 
SKSLVIVESPAKAKTINKYLGSQYVVKSSVGHIRDLPTVGSSTGEKAKPISTKGMDAEE
CCCEEEEECCCHHHHHHHHHCCHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCHHH
KAKIKAEKERSALVKRMGIDPYHDWKANYQILPGKEKVVSELKSLAKKADHIYLATDLDR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCEEEEEECCCC
EGEAIAWHLREVIGGNDDRFSRVVFNEITKNAIKQAFEKPEQLNMDRVNAQQTRRFLDRV
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHCCHHHHHHHHHHH
VGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEVAVLTNNQNKQAI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEE
RLDVTDYKGKKFDPKNQKEAQSAVDFLNVSDYVVTDLETKPTSSRPRAPFITSTLQQTAS
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH
TRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSQDALNMARSYIENHFGAQYLPEKPNF
HHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
YSSKENAQEAHEAIRPSDIRALPESLEGMEKDAVRLYDLIWRQFLACQMPPAQYDSSTLT
CCCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VTAGDYTLKAKGRILRFDGWTKVLPQIGKNPEDQELPSVTVSEKLALKEVQPTQHFTKPP
EEECCEEEEECCCEEEECCHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHCCCHHHCCCCC
ARFTEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLNESF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHH
GELMNYDFTANMEDTLDKIASGSVNWKTELNQFFKDFSSQLSKAELDELEGGMRPNNLVE
HHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
TDIKCPTCDRKMAIRTASTGVFLGCSGYALPPKERCKTTINLIPEAELLNVLDESSETKA
CCCCCCCCCCHHEEEEECCCEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCHHHH
LMDRKRCTKCGTAMDSYVIDAHRKIHICGNNPNCDGYLIEEGSFKIKGYDGPVVECDKCG
HHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCEEEEECCCEEEECCCCCEEEHHHCC
ADMHLKLGRFGKYMGCTNCDNTRKILKNGEVAPPKEEPVHFPELKCEKSDAYFVLRDGAS
CCCEEEHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCC
GVFMSAHNFPKSRETRPVKIAELVQYRERLPEKLAYLADAPQKDPEENEAIVRFSRKEKK
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
QYVTSEKEGKATKWIVDFTNGKWVERKK
HHHCCCCCCCCEEEEEECCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]