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The map label for this gene is rimK [H]

Identifier: 148826810

GI number: 148826810

Start: 48630

End: 49538

Strand: Direct

Name: rimK [H]

Synonym: CGSHiGG_00300

Alternate gene names: 148826810

Gene position: 48630-49538 (Clockwise)

Preceding gene: 148826808

Following gene: 148826814

Centisome position: 2.58

GC content: 38.39

Gene sequence:

>909_bases
ATGAAATTACTAATGCTTTGCCGAGAACCTCGTCTTTATAGTTGCCAACGCTTAAAAGAAGCGGCTAAACATCAAGGGCA
TGAGATGGATATTTTAGATCCGAATCACTGTTTCTTAAAACTCTCGCAAAATCCACCGCACTTTCAGATTTTTTATCAAG
AAAATTCTGAGTCGAAACCTTATTTATTGCCTGATTATGATGCAGTTTTGCCTCGATTTGGCACAACGAGTACGCAAATG
GGATGTTCAGTCTTACAACATTTTGAAGGCAAAGGGACTTTTTGTTTAAATTCATCTCAGGCATTTTTAAATGCACGTGA
TAAGTGGAAAAGTTTGCAACTTTTGCTGAAGGCTGGTGTTCCTGTTCCCAATTCACTTTTTAGTGGCGGTGAAGTACAAG
CTCATGCGACGATACCTCATATCAGTTCGCCGACAATTTTAAAAACGCTAAATGGTTCACAAGGAATTGGGGTAATTTTA
GCTGAAAAACCACAAAGTGCGGTGAGCATTATGGAAGCTTTTAAGCAAACCAATATATCTATGTTGCAACAAGATTTTAT
CGAAGAAGCGGGCAATGCGGATATTCGTTGTTTTGTGATGGGTGATCAAGTGGTCGCAACGATGCAGAGAATTGGGCAAA
ATGGTGAGTTTCGGGCGAATTGCCATCGTGGCGGAAAAACCGAAAAAATTACCCTAAGCGATGAAGAAAAGCAGATAGCC
ATTCAAGCAACAAAAGCTATCGGTTTAGATGTAGCTGGCGTGGATTTAATTCGTTCAAAAAATGGATTATTGGTTTTAGA
AGTGAATGCAAGCCCAGGCTTAGAAATGATTGAAAAAACAAGTGGGATTGATATTGCAGCTGAAATAATTGATTATATTG
AAATAAATGCCTTTATCAATTCAAGATAA

Upstream 100 bases:

>100_bases
TGAGTTAAAATTCTAGGTAATTCTACTGATTTTTATCATCTACATAAAGCGTTTTTATTCGTGTAATATAAGGAACATCG
TAGACAATGAGTTAAATATT

Downstream 100 bases:

>100_bases
AGGCATTTCTCTAAAACATTGTCAATTTCAACCGCACTTTACGCGAGCATATTTACAACTGCAAATGAAACTTTTAATAG
CGCAGCGTTGATTAAGTCGA

Product: ribosomal protein S6 modification protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKLLMLCREPRLYSCQRLKEAAKHQGHEMDILDPNHCFLKLSQNPPHFQIFYQENSESKPYLLPDYDAVLPRFGTTSTQM
GCSVLQHFEGKGTFCLNSSQAFLNARDKWKSLQLLLKAGVPVPNSLFSGGEVQAHATIPHISSPTILKTLNGSQGIGVIL
AEKPQSAVSIMEAFKQTNISMLQQDFIEEAGNADIRCFVMGDQVVATMQRIGQNGEFRANCHRGGKTEKITLSDEEKQIA
IQATKAIGLDVAGVDLIRSKNGLLVLEVNASPGLEMIEKTSGIDIAAEIIDYIEINAFINSR

Sequences:

>Translated_302_residues
MKLLMLCREPRLYSCQRLKEAAKHQGHEMDILDPNHCFLKLSQNPPHFQIFYQENSESKPYLLPDYDAVLPRFGTTSTQM
GCSVLQHFEGKGTFCLNSSQAFLNARDKWKSLQLLLKAGVPVPNSLFSGGEVQAHATIPHISSPTILKTLNGSQGIGVIL
AEKPQSAVSIMEAFKQTNISMLQQDFIEEAGNADIRCFVMGDQVVATMQRIGQNGEFRANCHRGGKTEKITLSDEEKQIA
IQATKAIGLDVAGVDLIRSKNGLLVLEVNASPGLEMIEKTSGIDIAAEIIDYIEINAFINSR
>Mature_302_residues
MKLLMLCREPRLYSCQRLKEAAKHQGHEMDILDPNHCFLKLSQNPPHFQIFYQENSESKPYLLPDYDAVLPRFGTTSTQM
GCSVLQHFEGKGTFCLNSSQAFLNARDKWKSLQLLLKAGVPVPNSLFSGGEVQAHATIPHISSPTILKTLNGSQGIGVIL
AEKPQSAVSIMEAFKQTNISMLQQDFIEEAGNADIRCFVMGDQVVATMQRIGQNGEFRANCHRGGKTEKITLSDEEKQIA
IQATKAIGLDVAGVDLIRSKNGLLVLEVNASPGLEMIEKTSGIDIAAEIIDYIEINAFINSR

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=232, Percent_Identity=31.4655172413793, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI134254462, Length=214, Percent_Identity=31.7757009345794, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1787076, Length=298, Percent_Identity=44.6308724832215, Blast_Score=264, Evalue=7e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 33306; Mature: 33306

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLMLCREPRLYSCQRLKEAAKHQGHEMDILDPNHCFLKLSQNPPHFQIFYQENSESKP
CEEEEEECCCCHHHHHHHHHHHHHCCCEECEECCCCEEEEECCCCCEEEEEEECCCCCCC
YLLPDYDAVLPRFGTTSTQMGCSVLQHFEGKGTFCLNSSQAFLNARDKWKSLQLLLKAGV
EECCCHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHCHHHHHHHHHHHHHCCC
PVPNSLFSGGEVQAHATIPHISSPTILKTLNGSQGIGVILAEKPQSAVSIMEAFKQTNIS
CCCHHHCCCCCEEEEEECCCCCCCCEEEECCCCCCEEEEEECCCHHHHHHHHHHHHCCHH
MLQQDFIEEAGNADIRCFVMGDQVVATMQRIGQNGEFRANCHRGGKTEKITLSDEEKQIA
HHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCCEEE
IQATKAIGLDVAGVDLIRSKNGLLVLEVNASPGLEMIEKTSGIDIAAEIIDYIEINAFIN
EEEEHHHCCEEECCCEEECCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHEEEEEEC
SR
CC
>Mature Secondary Structure
MKLLMLCREPRLYSCQRLKEAAKHQGHEMDILDPNHCFLKLSQNPPHFQIFYQENSESKP
CEEEEEECCCCHHHHHHHHHHHHHCCCEECEECCCCEEEEECCCCCEEEEEEECCCCCCC
YLLPDYDAVLPRFGTTSTQMGCSVLQHFEGKGTFCLNSSQAFLNARDKWKSLQLLLKAGV
EECCCHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHCHHHHHHHHHHHHHCCC
PVPNSLFSGGEVQAHATIPHISSPTILKTLNGSQGIGVILAEKPQSAVSIMEAFKQTNIS
CCCHHHCCCCCEEEEEECCCCCCCCEEEECCCCCCEEEEEECCCHHHHHHHHHHHHCCHH
MLQQDFIEEAGNADIRCFVMGDQVVATMQRIGQNGEFRANCHRGGKTEKITLSDEEKQIA
HHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCCEEE
IQATKAIGLDVAGVDLIRSKNGLLVLEVNASPGLEMIEKTSGIDIAAEIIDYIEINAFIN
EEEEHHHCCEEECCCEEECCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHEEEEEEC
SR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA