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The map label for this gene is folA [H]

Identifier: 148826456

GI number: 148826456

Start: 1491447

End: 1491929

Strand: Direct

Name: folA [H]

Synonym: CGSHiEE_07515

Alternate gene names: 148826456

Gene position: 1491447-1491929 (Clockwise)

Preceding gene: 148826449

Following gene: 148826457

Centisome position: 82.26

GC content: 38.3

Gene sequence:

>483_bases
ATGACATTTAGTTTAATTGTGGCGACAACATTAAATAATGTGATTGGTAAAGATAACCAAATGCCTTGGCATTTGCCTGC
AGATTTAGCTTGGTTTCGTCAGAACACCACTGGTAAACCTGTCATTATGGGGCGTAAAACCTTTGAAAGCATTGGTCGTC
CACTACCTAAACGTACCAATATCGTACTTTCTCGCCAGCTTTTTGAACACGACGGTGTGGTATGGAAAGGTAGCCTTGAA
AGTGCGGTCGATTTTGTCAGAGATTTTGATGAAATTATGCTGATTGGTGGGGGAGAGTTATTCAAACAATATTTGCCCAA
AGCAGATAAGTTATACCTTACTCAAATTCAAACAGAACTAGATGGCGATACTTTTTTCCCTCAATTGAATTGGGAGGAGT
GGGAAATTGAATTTGATGAATATCGTAAGGCAGATGAACAAAATCGCTATGATTGCCGATTTTTAATTCTTACTCGAAAA
TAA

Upstream 100 bases:

>100_bases
AGTCCTTTCCAAGTTCTTAAAATTGGCATTAACGTTATCACTAAATCAATGAAAAATCACTTAATATCAGGTATAGTAAC
GGCAAATTTTAGGGGGATTT

Downstream 100 bases:

>100_bases
ATCAAATAAATGAAACAATACTTCTAGAACATTCATTAATCTAGCAAAAACAAAGAGATGATAATTTCCCCTTGAGTTCC
CCTTTTTAAAGTTTCATAAT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTFSLIVATTLNNVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTNIVLSRQLFEHDGVVWKGSLE
SAVDFVRDFDEIMLIGGGELFKQYLPKADKLYLTQIQTELDGDTFFPQLNWEEWEIEFDEYRKADEQNRYDCRFLILTRK

Sequences:

>Translated_160_residues
MTFSLIVATTLNNVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTNIVLSRQLFEHDGVVWKGSLE
SAVDFVRDFDEIMLIGGGELFKQYLPKADKLYLTQIQTELDGDTFFPQLNWEEWEIEFDEYRKADEQNRYDCRFLILTRK
>Mature_159_residues
TFSLIVATTLNNVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTNIVLSRQLFEHDGVVWKGSLES
AVDFVRDFDEIMLIGGGELFKQYLPKADKLYLTQIQTELDGDTFFPQLNWEEWEIEFDEYRKADEQNRYDCRFLILTRK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=156, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI1786233, Length=158, Percent_Identity=53.7974683544304, Blast_Score=177, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17505899, Length=153, Percent_Identity=33.9869281045752, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324810, Length=123, Percent_Identity=36.5853658536585, Blast_Score=71, Evalue=9e-14,
Organism=Drosophila melanogaster, GI116008026, Length=147, Percent_Identity=36.734693877551, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647458, Length=147, Percent_Identity=36.734693877551, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18794; Mature: 18662

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSLIVATTLNNVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTN
CCEEEEHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCC
IVLSRQLFEHDGVVWKGSLESAVDFVRDFDEIMLIGGGELFKQYLPKADKLYLTQIQTEL
HHHHHHHHHHCCEEEECCHHHHHHHHHHHHHEEEECCHHHHHHHCCCCCCEEEEEHHHHC
DGDTFFPQLNWEEWEIEFDEYRKADEQNRYDCRFLILTRK
CCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TFSLIVATTLNNVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTN
CEEEEHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCC
IVLSRQLFEHDGVVWKGSLESAVDFVRDFDEIMLIGGGELFKQYLPKADKLYLTQIQTEL
HHHHHHHHHHCCEEEECCHHHHHHHHHHHHHEEEECCHHHHHHHCCCCCCEEEEEHHHHC
DGDTFFPQLNWEEWEIEFDEYRKADEQNRYDCRFLILTRK
CCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]