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The map label for this gene is glyS

Identifier: 148826424

GI number: 148826424

Start: 1460464

End: 1462530

Strand: Direct

Name: glyS

Synonym: CGSHiEE_07355

Alternate gene names: 148826424

Gene position: 1460464-1462530 (Clockwise)

Preceding gene: 148826423

Following gene: 148826425

Centisome position: 80.55

GC content: 44.46

Gene sequence:

>2067_bases
ATGACAACCCAAAACTTCCTAGTAGAAATCGGCACGGAAGAGCTGCCACCAAAAGCTCTCAAAACATTAGCAACCTCTTT
TGCGGATAACGTTGAGGCGGAATTAATCCAAGCGGGCTTATCATTCGACAAAATCGAATGGTTTGCGGCGCCGCGTCGTT
TAGCGGTGAAAGTGTTGAACTTGGCGACACAACAACCGAGCAAAGAAATCGAAAAACGAGGGCCTGCAGTATCAGCAGCC
TTTGATGCGGAAGGTAAACCAACTAAAGCGGCTGAAGGCTGGGCGCGTGGTTGTGGCATTACTGTTGAGCAAGCAGAACG
CATTGCGACTGATAAAGGTGAATGGCTCGTTCATTGTGCGAAAATTGAAGGCCAACCGACCAAAAACTTATTAAACGACA
TTGTGGCAAATGCGTTAGCGAAATTGCCAATTCCAAAACCAATGCGTTGGGCTGACAAAACTGTGCAATTTATTCGCCCA
GTTCATACTGTGACTATGTTGTTAGGTGATGAGTTAATTGAAGGCGAAATTTTAGGTGTAGCAAGTGCTCGCACCATTCG
CGGTCACCGTTTCTTAGGCGAGAAAGAATTTGAAATTCAACATGCAGACCAATATCCGCAATTATTGCGTGAAAAAGGTT
CTGTGGTAGCAGACTTCAATGAACGTAAAGCAGAAATTTTGGCAAAATCTCAAGCAAAAGCGACCGCACTTGGCGGCGTG
GCTGATATTGAAGAAAGCTTGCTTGAAGAAGTGACTTCGTTGGTGGAATATCCAAATGTTTTAGCAGCAAAATTTGAAGA
ACGTTTCTTAGCCGTGCCTGCGGAAGCCTTGGTTTACACCATGAAAGGCGACCAAAAATATTTCCCGATTTATGATAAAG
ACGGCAAATTATTACCGCACTTTATTTTTGTCTCGAACATCAATCCAGAAGATCCAACCGCAATTATCGAAGGGAACGAA
AAAGTGGTTCGCCCACGTTTAACCGATGCGGAATTCTTCTTCAAAACCGACTTAAAACAAAAACTGGTTGATCGTTTACC
GCGTTTAGAAACCGTGTTGTTCCAACAACAATTGGGTACATTGAAAGACAAAACTGACCGTATTGAACAACTTGCAGGCG
AAATTGCAAAACAAATAGGCGCAGACGAAGCGAAAGCAAAACGTGCTGGCTTACTGTCAAAATGTGATTTGATGACCAAC
ATGGTATTCGAATTCACTGATACGCAAGGCGTAATGGGTATGCATTATGCTCGTCACGACGGTGAAGATGAAGAAGTGGC
AGTGGCGTTAAACGAACAATATATGCCACGTTTTGCGGGTGATGAGTTACCAAAATCACTCGTTGCAAGTGCGGTTGCTT
TAGCGGATAAATTTGACACTTTAACAGGTATCTTCGGTATCGGCCAAGCACCAAAAGGTAGCGCAGACCCGTTCGCATTA
CGCCGTGCAGCATTAGGTGCATTGCGTATTATCGTCGAGAAAAACTTACCACTGGATTTAGAAGATTTAGTGAAAAAATC
AGCCGCACTTTTCAGCGATAAACTCACGAATAAAAACGTGGTTGCTGATGTGGTGGACTTCATGCTCGGTCGTTTCCGTG
CATGGTATCAAGATGAAGGCATTGCGGTGGATGTAATTCAAGCGGTATTGGCACGTCGTCCAACTCGCCCAGCTGATTTT
GATGCGCGTGTACGTGCGGTTTCACACTTCCGTACTTTAGATTCAGCGGAAGCGTTAGCAGCAGCAAACAAACGTGTAAG
CAACATCTTAGCCAAAGCGGATGCTGCAATTGGCGAGATCAATTTAACCGCTTGCGTAGAGCCAGCAGAAAAAGCCCTTG
CTGAAGCGGTACTTGTATTACGCACTGAAGTACAACCGCTTATCGCACAAGGCGATTACACTGCAGTGTTAGATAAATTA
GCAAACTTACGCGCACCGGTAGACAGCTTCTTTGATAACGTGATGGTAAATGCTGAAGATCCAGCATTACGCCAAAACCG
TTTGGCGATATTGAACACACTTCAAGGGTTATTCTTACAAGTAGCTGATATTTCAGTGCTTCAATAA

Upstream 100 bases:

>100_bases
ATCCCCTCTTTAGCAAAGAGGGGTAGGGGAGATTTGGCAGAAGTAATAACGAAGAAAATAAGAATTTAAATCTACAGAAT
TTTTAAATAAGAGAAACAAA

Downstream 100 bases:

>100_bases
TGATAAAAGGGATGGTTGATTTTTCGTCAATCATCCCTTCTTTTATAGACTTCAAGTGATTATTTCTCTATAATTACCGC
ACTTTCCCCCCCTTAGCTCA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MTTQNFLVEIGTEELPPKALKTLATSFADNVEAELIQAGLSFDKIEWFAAPRRLAVKVLNLATQQPSKEIEKRGPAVSAA
FDAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLVHCAKIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRP
VHTVTMLLGDELIEGEILGVASARTIRGHRFLGEKEFEIQHADQYPQLLREKGSVVADFNERKAEILAKSQAKATALGGV
ADIEESLLEEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPHFIFVSNINPEDPTAIIEGNE
KVVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGTLKDKTDRIEQLAGEIAKQIGADEAKAKRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFAL
RRAALGALRIIVEKNLPLDLEDLVKKSAALFSDKLTNKNVVADVVDFMLGRFRAWYQDEGIAVDVIQAVLARRPTRPADF
DARVRAVSHFRTLDSAEALAAANKRVSNILAKADAAIGEINLTACVEPAEKALAEAVLVLRTEVQPLIAQGDYTAVLDKL
ANLRAPVDSFFDNVMVNAEDPALRQNRLAILNTLQGLFLQVADISVLQ

Sequences:

>Translated_688_residues
MTTQNFLVEIGTEELPPKALKTLATSFADNVEAELIQAGLSFDKIEWFAAPRRLAVKVLNLATQQPSKEIEKRGPAVSAA
FDAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLVHCAKIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRP
VHTVTMLLGDELIEGEILGVASARTIRGHRFLGEKEFEIQHADQYPQLLREKGSVVADFNERKAEILAKSQAKATALGGV
ADIEESLLEEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPHFIFVSNINPEDPTAIIEGNE
KVVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGTLKDKTDRIEQLAGEIAKQIGADEAKAKRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFAL
RRAALGALRIIVEKNLPLDLEDLVKKSAALFSDKLTNKNVVADVVDFMLGRFRAWYQDEGIAVDVIQAVLARRPTRPADF
DARVRAVSHFRTLDSAEALAAANKRVSNILAKADAAIGEINLTACVEPAEKALAEAVLVLRTEVQPLIAQGDYTAVLDKL
ANLRAPVDSFFDNVMVNAEDPALRQNRLAILNTLQGLFLQVADISVLQ
>Mature_687_residues
TTQNFLVEIGTEELPPKALKTLATSFADNVEAELIQAGLSFDKIEWFAAPRRLAVKVLNLATQQPSKEIEKRGPAVSAAF
DAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLVHCAKIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRPV
HTVTMLLGDELIEGEILGVASARTIRGHRFLGEKEFEIQHADQYPQLLREKGSVVADFNERKAEILAKSQAKATALGGVA
DIEESLLEEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPHFIFVSNINPEDPTAIIEGNEK
VVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGTLKDKTDRIEQLAGEIAKQIGADEAKAKRAGLLSKCDLMTNM
VFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFALR
RAALGALRIIVEKNLPLDLEDLVKKSAALFSDKLTNKNVVADVVDFMLGRFRAWYQDEGIAVDVIQAVLARRPTRPADFD
ARVRAVSHFRTLDSAEALAAANKRVSNILAKADAAIGEINLTACVEPAEKALAEAVLVLRTEVQPLIAQGDYTAVLDKLA
NLRAPVDSFFDNVMVNAEDPALRQNRLAILNTLQGLFLQVADISVLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=69.66618287373, Blast_Score=969, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_HAEIE (A5UDE3)

Other databases:

- EMBL:   CP000671
- RefSeq:   YP_001291177.1
- ProteinModelPortal:   A5UDE3
- STRING:   A5UDE3
- GeneID:   5225730
- GenomeReviews:   CP000671_GR
- KEGG:   hip:CGSHiEE_07355
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- BioCyc:   HINF374930:CGSHIEE_07355-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75617; Mature: 75485

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQNFLVEIGTEELPPKALKTLATSFADNVEAELIQAGLSFDKIEWFAAPRRLAVKVLN
CCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LATQQPSKEIEKRGPAVSAAFDAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLVHCA
HHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
KIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRPVHTVTMLLGDELIEGEILGV
ECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ASARTIRGHRFLGEKEFEIQHADQYPQLLREKGSVVADFNERKAEILAKSQAKATALGGV
CCHHHHCCHHCCCCCCCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHCCH
ADIEESLLEEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHEEEEECCCCEECCEECCCCCCCCE
FIFVSNINPEDPTAIIEGNEKVVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGT
EEEEECCCCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LKDKTDRIEQLAGEIAKQIGADEAKAKRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
GEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFAL
CCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGALRIIVEKNLPLDLEDLVKKSAALFSDKLTNKNVVADVVDFMLGRFRAWYQDEG
HHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
IAVDVIQAVLARRPTRPADFDARVRAVSHFRTLDSAEALAAANKRVSNILAKADAAIGEI
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
NLTACVEPAEKALAEAVLVLRTEVQPLIAQGDYTAVLDKLANLRAPVDSFFDNVMVNAED
EEHEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCEEECCCC
PALRQNRLAILNTLQGLFLQVADISVLQ
CHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQNFLVEIGTEELPPKALKTLATSFADNVEAELIQAGLSFDKIEWFAAPRRLAVKVLN
CCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LATQQPSKEIEKRGPAVSAAFDAEGKPTKAAEGWARGCGITVEQAERIATDKGEWLVHCA
HHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
KIEGQPTKNLLNDIVANALAKLPIPKPMRWADKTVQFIRPVHTVTMLLGDELIEGEILGV
ECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ASARTIRGHRFLGEKEFEIQHADQYPQLLREKGSVVADFNERKAEILAKSQAKATALGGV
CCHHHHCCHHCCCCCCCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHCCH
ADIEESLLEEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHEEEEECCCCEECCEECCCCCCCCE
FIFVSNINPEDPTAIIEGNEKVVRPRLTDAEFFFKTDLKQKLVDRLPRLETVLFQQQLGT
EEEEECCCCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LKDKTDRIEQLAGEIAKQIGADEAKAKRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
GEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQAPKGSADPFAL
CCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGALRIIVEKNLPLDLEDLVKKSAALFSDKLTNKNVVADVVDFMLGRFRAWYQDEG
HHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
IAVDVIQAVLARRPTRPADFDARVRAVSHFRTLDSAEALAAANKRVSNILAKADAAIGEI
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
NLTACVEPAEKALAEAVLVLRTEVQPLIAQGDYTAVLDKLANLRAPVDSFFDNVMVNAED
EEHEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCEEECCCC
PALRQNRLAILNTLQGLFLQVADISVLQ
CHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA