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The map label for this gene is patB [H]

Identifier: 148826277

GI number: 148826277

Start: 1293089

End: 1294261

Strand: Direct

Name: patB [H]

Synonym: CGSHiEE_06520

Alternate gene names: 148826277

Gene position: 1293089-1294261 (Clockwise)

Preceding gene: 148826276

Following gene: 148826278

Centisome position: 71.32

GC content: 27.71

Gene sequence:

>1173_bases
ATGAATAATTTTTATAATTTTGATGTGATTATTGATCGCCTAAATACATTTAGTGCTAAATGGAATGTAGATAATGATAT
TATTCCAATGTCTGTTGCGGATATGGATATTCCAGCCCCTCAGATAATGATAGATGAATTAGAAAAATTTAATCGTTTAG
GAATTTATGGTTATACTGAATTACCAGCCGACTATTACAAGATAATCAGTGATTATATTTTCGAACAATATCATTATTCT
GTTCTCTCTGAAAAAATTGTATTTTGCCCAAGAATAATCCAAGCTGTATCTATTTATATACGAGCATTTACAAAAGAAAC
TGATGGAATATGCATTCTTTCACCTTCATATAGTCCAATATTAAACACGATAAAATTAAATGACAGAAAATTATATAAAT
GTCCTTTGGTTTATGAGAATAGAAAGTATCGTATTGATTTTAATTTATTAGAGGAATGTTTTGAACATTCTAAAGTATTT
GTTTTAATTTCTCCTCATAACCCAACTGGAACGATTTGGAAAAAAGAAGTCCTTATTAAAATTATAAACTTAGCTAAAAA
ACATAATGTATTTATCATTTCCGATGATGTCCACGCAGACTTTACACTAACAAATGACTCCCACTATTTAATATCATCAT
TAGATGAATGGGTAGCAAATCACTCAATGATCTGTTTATCCCCAGCAAAAACTTTTAATATTCCTGGATTGGAAATAGCT
AACCTAATTATTGAGAATAAAGAGATTCGAGAAAAATTTATAAAAGAAATGATGGCATTAGGTATACATAATCCAAATTA
TTTCTCTATACCAGCTATTATTTCGTTATATAAATATTGTACTGACTGGGTAATCTATCTAAAAGAATACATAAAGAATA
ATAAGAAGATAGTAAAAGAATTTTTCAATGAGTATATTCCATTATTAGATATTACTGAAAGTGATGGAACTTATTTACTT
TGGATAAACTATAAAAAGCTAAGTATAAATGAAAATGAATTAGCTTATTGGATTAATAATTTATCAAGAGTAAAAGTATC
TTTGGGGAGTGAATTTAGCAAAGAGGGAGACGGTTTCTTTCGTATGAATGTCGCTATGCCTAGAGAAAAATTATTAGAAG
CATTGAATCGAATCAAAAATGGGTTTTGTTTATTAAACAATAGGGAGATTTAA

Upstream 100 bases:

>100_bases
TCACAGTTGTGAAAAAACGTAATAGATAAAGTAGTTTTAGTAATTTTAATTCTTTTCTTCTTTGACTAAAGTAAATTTGC
TTTATACATCAGGAGGAATA

Downstream 100 bases:

>100_bases
TATGAAAACCACTCATCGCACAAGAATGCCAACAACATTAGAAGCTTTTTCACCAATCATTGTGATGCTATTACTGTTAG
GGCTTGGGTATGCTTTATTT

Product: putative aspartate aminotransferase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNNFYNFDVIIDRLNTFSAKWNVDNDIIPMSVADMDIPAPQIMIDELEKFNRLGIYGYTELPADYYKIISDYIFEQYHYS
VLSEKIVFCPRIIQAVSIYIRAFTKETDGICILSPSYSPILNTIKLNDRKLYKCPLVYENRKYRIDFNLLEECFEHSKVF
VLISPHNPTGTIWKKEVLIKIINLAKKHNVFIISDDVHADFTLTNDSHYLISSLDEWVANHSMICLSPAKTFNIPGLEIA
NLIIENKEIREKFIKEMMALGIHNPNYFSIPAIISLYKYCTDWVIYLKEYIKNNKKIVKEFFNEYIPLLDITESDGTYLL
WINYKKLSINENELAYWINNLSRVKVSLGSEFSKEGDGFFRMNVAMPREKLLEALNRIKNGFCLLNNREI

Sequences:

>Translated_390_residues
MNNFYNFDVIIDRLNTFSAKWNVDNDIIPMSVADMDIPAPQIMIDELEKFNRLGIYGYTELPADYYKIISDYIFEQYHYS
VLSEKIVFCPRIIQAVSIYIRAFTKETDGICILSPSYSPILNTIKLNDRKLYKCPLVYENRKYRIDFNLLEECFEHSKVF
VLISPHNPTGTIWKKEVLIKIINLAKKHNVFIISDDVHADFTLTNDSHYLISSLDEWVANHSMICLSPAKTFNIPGLEIA
NLIIENKEIREKFIKEMMALGIHNPNYFSIPAIISLYKYCTDWVIYLKEYIKNNKKIVKEFFNEYIPLLDITESDGTYLL
WINYKKLSINENELAYWINNLSRVKVSLGSEFSKEGDGFFRMNVAMPREKLLEALNRIKNGFCLLNNREI
>Mature_390_residues
MNNFYNFDVIIDRLNTFSAKWNVDNDIIPMSVADMDIPAPQIMIDELEKFNRLGIYGYTELPADYYKIISDYIFEQYHYS
VLSEKIVFCPRIIQAVSIYIRAFTKETDGICILSPSYSPILNTIKLNDRKLYKCPLVYENRKYRIDFNLLEECFEHSKVF
VLISPHNPTGTIWKKEVLIKIINLAKKHNVFIISDDVHADFTLTNDSHYLISSLDEWVANHSMICLSPAKTFNIPGLEIA
NLIIENKEIREKFIKEMMALGIHNPNYFSIPAIISLYKYCTDWVIYLKEYIKNNKKIVKEFFNEYIPLLDITESDGTYLL
WINYKKLSINENELAYWINNLSRVKVSLGSEFSKEGDGFFRMNVAMPREKLLEALNRIKNGFCLLNNREI

Specific function: Catalyzes the transformation of cystathionine to homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine [H]

COG id: COG1168

COG function: function code E; Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787909, Length=390, Percent_Identity=27.1794871794872, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1786816, Length=294, Percent_Identity=20.0680272108844, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17567663, Length=343, Percent_Identity=23.0320699708455, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322401, Length=270, Percent_Identity=25.5555555555556, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 45719; Mature: 45719

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNFYNFDVIIDRLNTFSAKWNVDNDIIPMSVADMDIPAPQIMIDELEKFNRLGIYGYTE
CCCEEEHHHEEEHHHCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECC
LPADYYKIISDYIFEQYHYSVLSEKIVFCPRIIQAVSIYIRAFTKETDGICILSPSYSPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHH
LNTIKLNDRKLYKCPLVYENRKYRIDFNLLEECFEHSKVFVLISPHNPTGTIWKKEVLIK
HHEEEECCCEEEECCEEECCCEEEEEHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHH
IINLAKKHNVFIISDDVHADFTLTNDSHYLISSLDEWVANHSMICLSPAKTFNIPGLEIA
HHHHHHHCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
NLIIENKEIREKFIKEMMALGIHNPNYFSIPAIISLYKYCTDWVIYLKEYIKNNKKIVKE
HHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FFNEYIPLLDITESDGTYLLWINYKKLSINENELAYWINNLSRVKVSLGSEFSKEGDGFF
HHHCCCCEEEEECCCCCEEEEEEEEEEECCCCHHHHHHCCHHEEEEECCCCCCCCCCCEE
RMNVAMPREKLLEALNRIKNGFCLLNNREI
EEEECCCHHHHHHHHHHHCCCEEEEECCCC
>Mature Secondary Structure
MNNFYNFDVIIDRLNTFSAKWNVDNDIIPMSVADMDIPAPQIMIDELEKFNRLGIYGYTE
CCCEEEHHHEEEHHHCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECC
LPADYYKIISDYIFEQYHYSVLSEKIVFCPRIIQAVSIYIRAFTKETDGICILSPSYSPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHH
LNTIKLNDRKLYKCPLVYENRKYRIDFNLLEECFEHSKVFVLISPHNPTGTIWKKEVLIK
HHEEEECCCEEEECCEEECCCEEEEEHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHH
IINLAKKHNVFIISDDVHADFTLTNDSHYLISSLDEWVANHSMICLSPAKTFNIPGLEIA
HHHHHHHCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
NLIIENKEIREKFIKEMMALGIHNPNYFSIPAIISLYKYCTDWVIYLKEYIKNNKKIVKE
HHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FFNEYIPLLDITESDGTYLLWINYKKLSINENELAYWINNLSRVKVSLGSEFSKEGDGFF
HHHCCCCEEEEECCCCCEEEEEEEEEEECCCCHHHHHHCCHHEEEEECCCCCCCCCCCEE
RMNVAMPREKLLEALNRIKNGFCLLNNREI
EEEECCCHHHHHHHHHHHCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]