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The map label for this gene is hphIR [H]

Identifier: 148826165

GI number: 148826165

Start: 1166980

End: 1167963

Strand: Direct

Name: hphIR [H]

Synonym: CGSHiEE_05870

Alternate gene names: 148826165

Gene position: 1166980-1167963 (Clockwise)

Preceding gene: 148826164

Following gene: 148826167

Centisome position: 64.37

GC content: 32.22

Gene sequence:

>984_bases
ATGTTAATTGATGGAATTACTTATTTTCCTATAGATACTAAAGAAAAGATTACTATTGCGGATTCATTTGTCGTTAGAGC
TAATAAAATAGGTCATGGAAATGGAGAGGCAAAATTATATATCGGCAATGAAAATGAGGAAAATAGATCATTTTTTGGGG
AGAATGGATTCATAGTTCGTTGTTTTTTACTAAAGCAGGATTTAATTAAATACTTAGATGAGACGAAAGAGGAATATTTA
TCTCCAGAACAGCTTTATCAAAATGCTAGTGAACTCCCTACATTGTGGTTAGAGCGCCGACAAAAGATTACCAAGCTGCC
TGAAATAATTTGGTTTGATATAGAAGAACAAACACAAATTGCAGGCCCAAGATTGTATGTTAAATTTAATAATAAACAAT
CAAAATTTGGATATGAACTTATCCGAGAACTTTCCTTACCTAATGTGACATATATTTCTATCGTAAAACTAGTTAACTCA
AAAACTCTAAAAGAACCTATTTTTTATATTCGTCTCTTTGCAGATTATTTCGGTGATGTTATCCACCCTGCTATTCTTGA
ACAAGAAGAAAAAAATATCCTAGGAGAACCTGATAGTCGTGAAAAAACAGAAAAATTAAGAGCACGGCAAGGTCAGGGAG
AATATAGGAAAAAACTGTTAGAACAATGTCCATTTTGTCCAATTACATTGATTTCTGATGATCGCCTTTTAATCGCAAGT
CATATTAAACCATGGGCAAAATCAAATGACTTTGAGAAAACAGATCCATATAACGGATTTATGTTTACACCAACCATAGA
TCATCTATTTGATCGTGGTTTCATTACCTTCACAGATAATCAAGAAATGCTACTCTCTCCTTTTCTATCCAAAATGACTT
ATTCAAAATTAGGATTAAGCGACAGAAAAAAATATCCAATGTTAAAGATTGAAGGGAGAACAGGATATTTAGAATATCAT
AGACAGGAAATTTGGAAGGGTTGA

Upstream 100 bases:

>100_bases
GTGATACACAAGCATACCGTCAAGCAGGAAATAGTATCGTTGTTGATGTTTTAATTGCTATTTTGAAACAAATTGATATT
ACAAAATATGGAGTATAATA

Downstream 100 bases:

>100_bases
CCCAATTAATAAATAGGCTATCTAAAAAATTTTCCGCCCTTTTTTATTCAAATCGATCACTTAATAAAAATTGTAAAACC
GCATCCATTCTCAAATGAGG

Product: putative type II restriction endonuclease

Products: NA

Alternate protein names: R.HphI; Endonuclease HphI; Type II restriction enzyme HphI [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MLIDGITYFPIDTKEKITIADSFVVRANKIGHGNGEAKLYIGNENEENRSFFGENGFIVRCFLLKQDLIKYLDETKEEYL
SPEQLYQNASELPTLWLERRQKITKLPEIIWFDIEEQTQIAGPRLYVKFNNKQSKFGYELIRELSLPNVTYISIVKLVNS
KTLKEPIFYIRLFADYFGDVIHPAILEQEEKNILGEPDSREKTEKLRARQGQGEYRKKLLEQCPFCPITLISDDRLLIAS
HIKPWAKSNDFEKTDPYNGFMFTPTIDHLFDRGFITFTDNQEMLLSPFLSKMTYSKLGLSDRKKYPMLKIEGRTGYLEYH
RQEIWKG

Sequences:

>Translated_327_residues
MLIDGITYFPIDTKEKITIADSFVVRANKIGHGNGEAKLYIGNENEENRSFFGENGFIVRCFLLKQDLIKYLDETKEEYL
SPEQLYQNASELPTLWLERRQKITKLPEIIWFDIEEQTQIAGPRLYVKFNNKQSKFGYELIRELSLPNVTYISIVKLVNS
KTLKEPIFYIRLFADYFGDVIHPAILEQEEKNILGEPDSREKTEKLRARQGQGEYRKKLLEQCPFCPITLISDDRLLIAS
HIKPWAKSNDFEKTDPYNGFMFTPTIDHLFDRGFITFTDNQEMLLSPFLSKMTYSKLGLSDRKKYPMLKIEGRTGYLEYH
RQEIWKG
>Mature_327_residues
MLIDGITYFPIDTKEKITIADSFVVRANKIGHGNGEAKLYIGNENEENRSFFGENGFIVRCFLLKQDLIKYLDETKEEYL
SPEQLYQNASELPTLWLERRQKITKLPEIIWFDIEEQTQIAGPRLYVKFNNKQSKFGYELIRELSLPNVTYISIVKLVNS
KTLKEPIFYIRLFADYFGDVIHPAILEQEEKNILGEPDSREKTEKLRARQGQGEYRKKLLEQCPFCPITLISDDRLLIAS
HIKPWAKSNDFEKTDPYNGFMFTPTIDHLFDRGFITFTDNQEMLLSPFLSKMTYSKLGLSDRKKYPMLKIEGRTGYLEYH
RQEIWKG

Specific function: Recognizes the double-stranded sequences GGTGA and TCACC and cleaves respectively 13 bases after G-1 and 7 bases before T- 1, leaving a single 3' protruding nucleotide [H]

COG id: COG3440

COG function: function code V; Predicted restriction endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 38388; Mature: 38388

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDGITYFPIDTKEKITIADSFVVRANKIGHGNGEAKLYIGNENEENRSFFGENGFIVR
CEECCEEEECCCCCCEEEEECCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEE
CFLLKQDLIKYLDETKEEYLSPEQLYQNASELPTLWLERRQKITKLPEIIWFDIEEQTQI
HHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHC
AGPRLYVKFNNKQSKFGYELIRELSLPNVTYISIVKLVNSKTLKEPIFYIRLFADYFGDV
CCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
IHPAILEQEEKNILGEPDSREKTEKLRARQGQGEYRKKLLEQCPFCPITLISDDRLLIAS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCEEEEH
HIKPWAKSNDFEKTDPYNGFMFTPTIDHLFDRGFITFTDNQEMLLSPFLSKMTYSKLGLS
HCCCCCCCCCCCCCCCCCCEEECCCHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCC
DRKKYPMLKIEGRTGYLEYHRQEIWKG
CCCCCCEEEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLIDGITYFPIDTKEKITIADSFVVRANKIGHGNGEAKLYIGNENEENRSFFGENGFIVR
CEECCEEEECCCCCCEEEEECCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEE
CFLLKQDLIKYLDETKEEYLSPEQLYQNASELPTLWLERRQKITKLPEIIWFDIEEQTQI
HHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHC
AGPRLYVKFNNKQSKFGYELIRELSLPNVTYISIVKLVNSKTLKEPIFYIRLFADYFGDV
CCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
IHPAILEQEEKNILGEPDSREKTEKLRARQGQGEYRKKLLEQCPFCPITLISDDRLLIAS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCEEEEH
HIKPWAKSNDFEKTDPYNGFMFTPTIDHLFDRGFITFTDNQEMLLSPFLSKMTYSKLGLS
HCCCCCCCCCCCCCCCCCCEEECCCHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCC
DRKKYPMLKIEGRTGYLEYHRQEIWKG
CCCCCCEEEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]