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The map label for this gene is hpaIIM [H]

Identifier: 148826164

GI number: 148826164

Start: 1165748

End: 1166977

Strand: Direct

Name: hpaIIM [H]

Synonym: CGSHiEE_05865

Alternate gene names: 148826164

Gene position: 1165748-1166977 (Clockwise)

Preceding gene: 148826161

Following gene: 148826165

Centisome position: 64.3

GC content: 33.58

Gene sequence:

>1230_bases
GTGTCAAATTTACCATATAAAAATCAAGAGAATTATTCCCTTCCTTTCTCCTTTCAATATGAAAACTGGCGGAATAAACC
TTTTTCAAATCCCAGTTCTCATATTCGTTTAGCAACTGTTTTTAGCGGCATCGGTGCGATTGAACAGGCTATGAAACGTT
TGAAATTAAATCATGAAATTATATTTGCTGGAGATATTGACCCTCATGTAAAAAAAAGTTATTTAGCAAATTATCAATTA
TCAGAAGAGCACTGGCATTCTGACATTACAACCTTTGATGCGACGCCTTACCGAGAACAAATAGATTTATTAGTTGGTGG
AAGCCCTTGTCAAGCGTTCTCCATGGTAGGTAAAAGGCATGGTTTGGAGGATACTCGTGGTACTTTATTCTATGATTTTG
CCCGTGTAGTCAATGAAACAAAGCCAAAAATTTTTATTTATGAAAACGTAAAGGGATTATTGAATCATGATAATGGCAAA
ACATGGCAGGTAGTAAAAGATGTATTACATGCACTAGGTTATGATTTATATTTTCAAGTAATGAACAGCAAAAATTATGG
TATACCACAACATCGTGAGCGTATTTTCGTGGTTGGTTTTAAAACTCCTCCCCAAGGGGGATTTATGTTTCCAGCAGAAA
TTCCTCTTGAGCATTCAATGCAAGATTTCCTAGAAGATTACACAGATAGCAAATATTACCTAAAAGAAAAAGGAATTAAA
TTCGTAACTAGCTCTAAAAATCGCCAAAAACGGTACACTCAAATAAACGGTGATGTAATGCTATGCCAAAAAGCCAATCA
ACAATTTAACTGGCACGGGGATTTCATATTTGAATATGCTGTTAATTGTAGTAAATTTGATGAGTTTATTTTTAATGTAA
ATGATGTAGAAGAAAAATATTATTTATCAAATAAAATCCGCGATTATGTTTTAGCTGGTGGTACAAAGAATTTTAAAACT
AGTACAGAAACCGACTTAACTGTTGCAAGACCATTATTACAAACAATGCATAAAATGCATCGCGCTGGTGTTGATAATTA
TGTTACTCATAATCAAGGTCGCATTAGGAAATTAACACCAAGAGAATGCCTCCGACTTATGGGCTTTAGAGATGATTTCC
AAATTGTTGTAAGTGATACACAAGCATACCGTCAAGCAGGAAATAGTATCGTTGTTGATGTTTTAATTGCTATTTTGAAA
CAAATTGATATTACAAAATATGGAGTATAA

Upstream 100 bases:

>100_bases
GAGTTCGTCTGAAAATATTTCCCATGAATATCCCACTGACAGCTATAAGAAATTTTTGTTATAATTAACAAAGACTTTCG
AATTTATTAGAGATGATTTT

Downstream 100 bases:

>100_bases
TAATGTTAATTGATGGAATTACTTATTTTCCTATAGATACTAAAGAAAAGATTACTATTGCGGATTCATTTGTCGTTAGA
GCTAATAAAATAGGTCATGG

Product: modification methylase

Products: NA

Alternate protein names: M.HpaII; Cytosine-specific methyltransferase HpaII [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSNLPYKNQENYSLPFSFQYENWRNKPFSNPSSHIRLATVFSGIGAIEQAMKRLKLNHEIIFAGDIDPHVKKSYLANYQL
SEEHWHSDITTFDATPYREQIDLLVGGSPCQAFSMVGKRHGLEDTRGTLFYDFARVVNETKPKIFIYENVKGLLNHDNGK
TWQVVKDVLHALGYDLYFQVMNSKNYGIPQHRERIFVVGFKTPPQGGFMFPAEIPLEHSMQDFLEDYTDSKYYLKEKGIK
FVTSSKNRQKRYTQINGDVMLCQKANQQFNWHGDFIFEYAVNCSKFDEFIFNVNDVEEKYYLSNKIRDYVLAGGTKNFKT
STETDLTVARPLLQTMHKMHRAGVDNYVTHNQGRIRKLTPRECLRLMGFRDDFQIVVSDTQAYRQAGNSIVVDVLIAILK
QIDITKYGV

Sequences:

>Translated_409_residues
MSNLPYKNQENYSLPFSFQYENWRNKPFSNPSSHIRLATVFSGIGAIEQAMKRLKLNHEIIFAGDIDPHVKKSYLANYQL
SEEHWHSDITTFDATPYREQIDLLVGGSPCQAFSMVGKRHGLEDTRGTLFYDFARVVNETKPKIFIYENVKGLLNHDNGK
TWQVVKDVLHALGYDLYFQVMNSKNYGIPQHRERIFVVGFKTPPQGGFMFPAEIPLEHSMQDFLEDYTDSKYYLKEKGIK
FVTSSKNRQKRYTQINGDVMLCQKANQQFNWHGDFIFEYAVNCSKFDEFIFNVNDVEEKYYLSNKIRDYVLAGGTKNFKT
STETDLTVARPLLQTMHKMHRAGVDNYVTHNQGRIRKLTPRECLRLMGFRDDFQIVVSDTQAYRQAGNSIVVDVLIAILK
QIDITKYGV
>Mature_408_residues
SNLPYKNQENYSLPFSFQYENWRNKPFSNPSSHIRLATVFSGIGAIEQAMKRLKLNHEIIFAGDIDPHVKKSYLANYQLS
EEHWHSDITTFDATPYREQIDLLVGGSPCQAFSMVGKRHGLEDTRGTLFYDFARVVNETKPKIFIYENVKGLLNHDNGKT
WQVVKDVLHALGYDLYFQVMNSKNYGIPQHRERIFVVGFKTPPQGGFMFPAEIPLEHSMQDFLEDYTDSKYYLKEKGIKF
VTSSKNRQKRYTQINGDVMLCQKANQQFNWHGDFIFEYAVNCSKFDEFIFNVNDVEEKYYLSNKIRDYVLAGGTKNFKTS
TETDLTVARPLLQTMHKMHRAGVDNYVTHNQGRIRKLTPRECLRLMGFRDDFQIVVSDTQAYRQAGNSIVVDVLIAILKQ
IDITKYGV

Specific function: This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HpaII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=210, Percent_Identity=31.4285714285714, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 47470; Mature: 47338

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLPYKNQENYSLPFSFQYENWRNKPFSNPSSHIRLATVFSGIGAIEQAMKRLKLNHEI
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEE
IFAGDIDPHVKKSYLANYQLSEEHWHSDITTFDATPYREQIDLLVGGSPCQAFSMVGKRH
EEECCCCHHHHHHHHHCCCCCHHHHCCCCEECCCCCCHHHEEEEECCCCHHHHHHHHHHC
GLEDTRGTLFYDFARVVNETKPKIFIYENVKGLLNHDNGKTWQVVKDVLHALGYDLYFQV
CCCCCCCHHHHHHHHHHCCCCCEEEEEECCHHHEECCCCCHHHHHHHHHHHHCHHHEEHE
MNSKNYGIPQHRERIFVVGFKTPPQGGFMFPAEIPLEHSMQDFLEDYTDSKYYLKEKGIK
ECCCCCCCCCCCCEEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHCCCEEEEHHCCCE
FVTSSKNRQKRYTQINGDVMLCQKANQQFNWHGDFIFEYAVNCSKFDEFIFNVNDVEEKY
EEECCCCHHHHHEEECCCEEEEECCCCCCCCCCCCCEEHHCCHHHHHHHHCCCCHHHHHH
YLSNKIRDYVLAGGTKNFKTSTETDLTVARPLLQTMHKMHRAGVDNYVTHNQGRIRKLTP
HHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEECCH
RECLRLMGFRDDFQIVVSDTQAYRQAGNSIVVDVLIAILKQIDITKYGV
HHHHHHHCCCCCEEEEEECHHHHHHCCCCHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
SNLPYKNQENYSLPFSFQYENWRNKPFSNPSSHIRLATVFSGIGAIEQAMKRLKLNHEI
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEE
IFAGDIDPHVKKSYLANYQLSEEHWHSDITTFDATPYREQIDLLVGGSPCQAFSMVGKRH
EEECCCCHHHHHHHHHCCCCCHHHHCCCCEECCCCCCHHHEEEEECCCCHHHHHHHHHHC
GLEDTRGTLFYDFARVVNETKPKIFIYENVKGLLNHDNGKTWQVVKDVLHALGYDLYFQV
CCCCCCCHHHHHHHHHHCCCCCEEEEEECCHHHEECCCCCHHHHHHHHHHHHCHHHEEHE
MNSKNYGIPQHRERIFVVGFKTPPQGGFMFPAEIPLEHSMQDFLEDYTDSKYYLKEKGIK
ECCCCCCCCCCCCEEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHCCCEEEEHHCCCE
FVTSSKNRQKRYTQINGDVMLCQKANQQFNWHGDFIFEYAVNCSKFDEFIFNVNDVEEKY
EEECCCCHHHHHEEECCCEEEEECCCCCCCCCCCCCEEHHCCHHHHHHHHCCCCHHHHHH
YLSNKIRDYVLAGGTKNFKTSTETDLTVARPLLQTMHKMHRAGVDNYVTHNQGRIRKLTP
HHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEECCH
RECLRLMGFRDDFQIVVSDTQAYRQAGNSIVVDVLIAILKQIDITKYGV
HHHHHHHCCCCCEEEEEECHHHHHHCCCCHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2183189; 7514149 [H]