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The map label for this gene is purK [H]

Identifier: 148826142

GI number: 148826142

Start: 1147749

End: 1148837

Strand: Direct

Name: purK [H]

Synonym: CGSHiEE_05745

Alternate gene names: 148826142

Gene position: 1147749-1148837 (Clockwise)

Preceding gene: 148826141

Following gene: 148826143

Centisome position: 63.31

GC content: 38.75

Gene sequence:

>1089_bases
ATGCAAAACTCCACCTTATACCCAACGGTTTATGTACTTGGCAACGGACAACTCGGCAGAATGTTAGGTTACGCTGGTGC
ACCTTTAGATATTTATGTTGAACCCTTAGCCTTCAATGCGCCAGTTTTTGACTTACCTGAAAATGCGATCATCACGGCAG
AAATTGAACGCTGGGAAAAAACACCTTTGACTGAATTACTCGGCAATCATAAAAACTTCGTTAATCAACATGTATTTGGA
TTATTAGCCGATCGTTTTGCTCAAAAATCCTTGCTTGATGAACTCAATCTTTCTACCTCGCCTTGGTGTTTATTAAAAGA
TAAAACGCAATGGAATGATGTTTTCCAAACTGTTGGCGAGAAAGTCGTCGTCAAACGTAGAACAGGCGGATATGATGGGC
GTGGTCAATGGATTATCAGCGATGAAAACAAAGCGGACATCACTGATGACTTATTTGGTGAAGTGATCGCAGAAAAATTT
ATTCCTTTTGATTATGAAGTTTCTATTGTAGGCGCAAGATTTAAAAATGGCGAAAAGCGTTTTTATCCCGTTACGCATAA
CCTGCAACAAAATGGTATTTTGCGTTACAGTGTGATTGATAATGCATTTCCTCAACAATCCGCACAACAAAAACAAGCTG
AAACAATGCTCGGCAAAATTATGGATAAGTTAGGCTATGTGGGTGTGATGGCAATGGAATGTTTTGTTGTAGGCGACAAA
TTACTAATTAACGAACTTGCGCCTCGCGTACATAACAGTGGACATTGGACACAACTGGGATGTGCTATTAGTCAATTTGA
ATTACATTTACGCACGCTACTTGATTTGCCAACACCTGACTTACAAATTTTTGCCCCAAGTGTAATGGTAAATTTAATCG
GCACAAACCATAATCCAAAATGGCTTAATATACCTTTTGCACAACTTCATTGGTACGGTAAAGAAGTGAGGGCTGGGCGT
AAGGTTGGGCATATCAATCTTTCTCATCCTAATAAAGCGGTCATTATTCAACAATTAGAAAAGCTCCGAATTGAATTACC
CGAAGATTATCAATCAGGATTAAATTGGGCGATTGAAAAACTAAAATAA

Upstream 100 bases:

>100_bases
TGTGCTTGATAATCCCGATCCACGCACATAAAAATATCTTCAGATCTGACCGCACTTTTAAAGTGCGGTTGTTTTTTTCG
TAAATTTAACGAGAAATATT

Downstream 100 bases:

>100_bases
TGTAAAAAATGACCGCACTTTTGATAAATATTTAAAAATATTAAAGTGCGGTTAATTTTTATAAAAATCTTTCTTGATAA
AATAAAACGGCTGAAGTATA

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MQNSTLYPTVYVLGNGQLGRMLGYAGAPLDIYVEPLAFNAPVFDLPENAIITAEIERWEKTPLTELLGNHKNFVNQHVFG
LLADRFAQKSLLDELNLSTSPWCLLKDKTQWNDVFQTVGEKVVVKRRTGGYDGRGQWIISDENKADITDDLFGEVIAEKF
IPFDYEVSIVGARFKNGEKRFYPVTHNLQQNGILRYSVIDNAFPQQSAQQKQAETMLGKIMDKLGYVGVMAMECFVVGDK
LLINELAPRVHNSGHWTQLGCAISQFELHLRTLLDLPTPDLQIFAPSVMVNLIGTNHNPKWLNIPFAQLHWYGKEVRAGR
KVGHINLSHPNKAVIIQQLEKLRIELPEDYQSGLNWAIEKLK

Sequences:

>Translated_362_residues
MQNSTLYPTVYVLGNGQLGRMLGYAGAPLDIYVEPLAFNAPVFDLPENAIITAEIERWEKTPLTELLGNHKNFVNQHVFG
LLADRFAQKSLLDELNLSTSPWCLLKDKTQWNDVFQTVGEKVVVKRRTGGYDGRGQWIISDENKADITDDLFGEVIAEKF
IPFDYEVSIVGARFKNGEKRFYPVTHNLQQNGILRYSVIDNAFPQQSAQQKQAETMLGKIMDKLGYVGVMAMECFVVGDK
LLINELAPRVHNSGHWTQLGCAISQFELHLRTLLDLPTPDLQIFAPSVMVNLIGTNHNPKWLNIPFAQLHWYGKEVRAGR
KVGHINLSHPNKAVIIQQLEKLRIELPEDYQSGLNWAIEKLK
>Mature_362_residues
MQNSTLYPTVYVLGNGQLGRMLGYAGAPLDIYVEPLAFNAPVFDLPENAIITAEIERWEKTPLTELLGNHKNFVNQHVFG
LLADRFAQKSLLDELNLSTSPWCLLKDKTQWNDVFQTVGEKVVVKRRTGGYDGRGQWIISDENKADITDDLFGEVIAEKF
IPFDYEVSIVGARFKNGEKRFYPVTHNLQQNGILRYSVIDNAFPQQSAQQKQAETMLGKIMDKLGYVGVMAMECFVVGDK
LLINELAPRVHNSGHWTQLGCAISQFELHLRTLLDLPTPDLQIFAPSVMVNLIGTNHNPKWLNIPFAQLHWYGKEVRAGR
KVGHINLSHPNKAVIIQQLEKLRIELPEDYQSGLNWAIEKLK

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=353, Percent_Identity=54.6742209631728, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1788155, Length=357, Percent_Identity=25.4901960784314, Blast_Score=83, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324702, Length=388, Percent_Identity=30.4123711340206, Blast_Score=142, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41026; Mature: 41026

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSTLYPTVYVLGNGQLGRMLGYAGAPLDIYVEPLAFNAPVFDLPENAIITAEIERWEK
CCCCEECEEEEEEECCCHHHHHHCCCCCEEEEEECEEECCCCEECCCCCEEEEEHHHCCC
TPLTELLGNHKNFVNQHVFGLLADRFAQKSLLDELNLSTSPWCLLKDKTQWNDVFQTVGE
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCC
KVVVKRRTGGYDGRGQWIISDENKADITDDLFGEVIAEKFIPFDYEVSIVGARFKNGEKR
EEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCE
FYPVTHNLQQNGILRYSVIDNAFPQQSAQQKQAETMLGKIMDKLGYVGVMAMECFVVGDK
EEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LLINELAPRVHNSGHWTQLGCAISQFELHLRTLLDLPTPDLQIFAPSVMVNLIGTNHNPK
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHEEEEEECCCCCC
WLNIPFAQLHWYGKEVRAGRKVGHINLSHPNKAVIIQQLEKLRIELPEDYQSGLNWAIEK
EEECCHHHHHHCCHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LK
CC
>Mature Secondary Structure
MQNSTLYPTVYVLGNGQLGRMLGYAGAPLDIYVEPLAFNAPVFDLPENAIITAEIERWEK
CCCCEECEEEEEEECCCHHHHHHCCCCCEEEEEECEEECCCCEECCCCCEEEEEHHHCCC
TPLTELLGNHKNFVNQHVFGLLADRFAQKSLLDELNLSTSPWCLLKDKTQWNDVFQTVGE
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCC
KVVVKRRTGGYDGRGQWIISDENKADITDDLFGEVIAEKFIPFDYEVSIVGARFKNGEKR
EEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCE
FYPVTHNLQQNGILRYSVIDNAFPQQSAQQKQAETMLGKIMDKLGYVGVMAMECFVVGDK
EEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LLINELAPRVHNSGHWTQLGCAISQFELHLRTLLDLPTPDLQIFAPSVMVNLIGTNHNPK
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHEEEEEECCCCCC
WLNIPFAQLHWYGKEVRAGRKVGHINLSHPNKAVIIQQLEKLRIELPEDYQSGLNWAIEK
EEECCHHHHHHCCHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]