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The map label for this gene is purU [H]

Identifier: 148826108

GI number: 148826108

Start: 1105577

End: 1106413

Strand: Direct

Name: purU [H]

Synonym: CGSHiEE_05545

Alternate gene names: 148826108

Gene position: 1105577-1106413 (Clockwise)

Preceding gene: 148826107

Following gene: 148826109

Centisome position: 60.98

GC content: 35.01

Gene sequence:

>837_bases
ATGATCGAAAAAAAAATATTACTCACTGATTGCCCTGATGATAAAGGCTTAATCGCAAAAATAACTAATATTTGTTACAA
ACATCAACTAAATATTCTTCATAATAATGAATTTGTAGATTTTGAAACCAAACATTTCTTTATGCGCACAGAATTGGAAG
GTATTTTTAACGAAGCTACTTTATTAGAAGATCTAAAATATAGCTTACCCGAAGAAACGAATTGTCGATTAATTGGCACC
CAACGTAAGCGAATTGTAATTTTAGTCACCAAAGAAGCTCACTGCCTCGGCGATATTTTAATGAAAAATTATTATGGTGC
GCTTGATGTAGAAATTGCAGCCGTAATCGGCAATCACGATAATTTACGTGAACTTGTTGAACGTTTTAATATTCCATTTC
ATTTAGTTAGCCACGAAAACTTAACCCGTGTAGAACACGACAAATTACTGGCAGAAAAAATTGATGAATATACGCCAGAT
TATATTGTTCTCGCTAAATATATGCGTGTGCTAAACCCTGAATTTGTCGCACGTTATCCAAATCGAGTGATTAATATCCA
TCATTCATTCTTGCCTGCTTTTATTGGTGCCAAGCCTTATCAACAAGCCTATGAACGCGGTGTAAAAATTATTGGCGCAA
CGGCACATTTTATTAATAACGAATTAGATCAAGGCCCAATCATTATGCAAAATGTGATTAATGTGGATCATACTTATAAT
GCAGAAGCGATGATGCGTGCTGGCCGTGATGTGGAAAAAACCGTGTTAAGTCGCGCCCTTGATCTCGCTTTACACGATAG
AATTTTTGTTTATAAAAATAAAACGGTGGTGTTGTAA

Upstream 100 bases:

>100_bases
AGGTAAATCATTATCTGATTTCGAAATTTAATTTTTCTATTTGAAACTAGAACCTATTTTATGGGTTCTATTTTTTTATC
TAAACATAGCACACAACATT

Downstream 100 bases:

>100_bases
TGGAAAAAATTACTCTTGCACCAATAAGTGCGGTTGAAGGAACAATTAATTTACCAGGTTCTAAAAGTTTATCGAATCGC
GCATTACTTTTAGCCGCCTT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MIEKKILLTDCPDDKGLIAKITNICYKHQLNILHNNEFVDFETKHFFMRTELEGIFNEATLLEDLKYSLPEETNCRLIGT
QRKRIVILVTKEAHCLGDILMKNYYGALDVEIAAVIGNHDNLRELVERFNIPFHLVSHENLTRVEHDKLLAEKIDEYTPD
YIVLAKYMRVLNPEFVARYPNRVINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL

Sequences:

>Translated_278_residues
MIEKKILLTDCPDDKGLIAKITNICYKHQLNILHNNEFVDFETKHFFMRTELEGIFNEATLLEDLKYSLPEETNCRLIGT
QRKRIVILVTKEAHCLGDILMKNYYGALDVEIAAVIGNHDNLRELVERFNIPFHLVSHENLTRVEHDKLLAEKIDEYTPD
YIVLAKYMRVLNPEFVARYPNRVINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL
>Mature_278_residues
MIEKKILLTDCPDDKGLIAKITNICYKHQLNILHNNEFVDFETKHFFMRTELEGIFNEATLLEDLKYSLPEETNCRLIGT
QRKRIVILVTKEAHCLGDILMKNYYGALDVEIAAVIGNHDNLRELVERFNIPFHLVSHENLTRVEHDKLLAEKIDEYTPD
YIVLAKYMRVLNPEFVARYPNRVINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=185, Percent_Identity=28.6486486486486, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI209869995, Length=185, Percent_Identity=28.6486486486486, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI209869993, Length=185, Percent_Identity=28.6486486486486, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=72.9241877256318, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1788846, Length=154, Percent_Identity=34.4155844155844, Blast_Score=91, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=217, Percent_Identity=31.3364055299539, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24582400, Length=192, Percent_Identity=27.0833333333333, Blast_Score=81, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32175; Mature: 32175

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKKILLTDCPDDKGLIAKITNICYKHQLNILHNNEFVDFETKHFFMRTELEGIFNEAT
CCCCEEEEEECCCCCCHHHHHHHHHHHHHEEEEECCEEEEECHHHEEHHHHHHHHHHHHH
LLEDLKYSLPEETNCRLIGTQRKRIVILVTKEAHCLGDILMKNYYGALDVEIAAVIGNHD
HHHHHHHCCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEEEECCCH
NLRELVERFNIPFHLVSHENLTRVEHDKLLAEKIDEYTPDYIVLAKYMRVLNPEFVARYP
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHCC
NRVINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
CCEEEEHHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHHCCCCCCC
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL
HHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEECCEEEC
>Mature Secondary Structure
MIEKKILLTDCPDDKGLIAKITNICYKHQLNILHNNEFVDFETKHFFMRTELEGIFNEAT
CCCCEEEEEECCCCCCHHHHHHHHHHHHHEEEEECCEEEEECHHHEEHHHHHHHHHHHHH
LLEDLKYSLPEETNCRLIGTQRKRIVILVTKEAHCLGDILMKNYYGALDVEIAAVIGNHD
HHHHHHHCCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEEEECCCH
NLRELVERFNIPFHLVSHENLTRVEHDKLLAEKIDEYTPDYIVLAKYMRVLNPEFVARYP
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHCC
NRVINIHHSFLPAFIGAKPYQQAYERGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
CCEEEEHHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHHCCCCCCC
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL
HHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 8335255 [H]