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The map label for this gene is pepT [H]

Identifier: 148825888

GI number: 148825888

Start: 883168

End: 884406

Strand: Direct

Name: pepT [H]

Synonym: CGSHiEE_04265

Alternate gene names: 148825888

Gene position: 883168-884406 (Clockwise)

Preceding gene: 148825883

Following gene: 148825890

Centisome position: 48.71

GC content: 36.88

Gene sequence:

>1239_bases
ATGATTTCTCAAATAGATAAAACAGAACTTTTAGAACGATTTTTACATTATGTGTCTTTTCACACCCAATCAAAACCTAA
TGCGAAGCATTCCCCCAGCTCTGTTGGGCAAATGAAGTTAGCCATGCAGTTACAAAAAGAATTAATCCAATTTGGTTTGG
AGAATGTTGAAGTAAGTAAATATGCAGTGGTAACAGCATTTTTACCCGCTAATGATCCTAACTTAACGAAAACCATTGGT
TTAGTTGCGCATCTTGATACATCTCCGCAATGTAGTGGTAAAAATGTACGCCTAGAAGTGATAGAAGAATATCGTGGCGG
AGATATTGCATTAGGTATTGGCGAGGAATTTATTAGCCCTGTTTATTATTCTTTTATGCAAAAATTAGTCGGACAAACGC
TTATTGTTACTGATGGAACCACTTTGCTTGGGGCGGATAATAAGGCAGGAATTGCAGAAATTATGACCGCACTTTCCATT
CTTCAAAAAGAGAATATTCCCCATTGCAATATTCGTGTTGCTTTTACGCCTGATGAAGAAATTGGTTTGGGAATCCATTA
TTTCCCAATGGAAAAGTTTTCCTGTGATTGGGCATATACCATTGATGGGGGAGAAGTAGGGGAATTAGAATACGAAAACT
TTAATGCTGCAACGGCAAAAGTGCGGTTTTTCGGGCGAAATATTCACACAGGTTATGCAAAAGGAAAAATGCTGAATGCG
CTGACTTTAGCTTGTGAATTTCAGCAAGTTTTTCCTGTTGATGAAGTACCAGAAAAAACCGATGGGAAAGCGGGCTTTTA
TCATTTAGAAGATTTTTTTGGCGATATTGAGCAAGTAGAGCTTACCTATTTGATTCGTGATTTTGACGAACAGAATTTCG
CACAAAGAAAAGCTTTTATTAAAAATCAGGTGGAAAAATTTAATGCTAAAAAAGGTTTGAAAAAGCCTATAGAGTTAGAA
ATTCAAGATAGCTACCAAAATATGTATGATGTGGTAAAAAACGTTCCTCAATCAATTGAACTTGCTGATCGCGCAATGAA
AGCGGTGGGAATTAAACCAAATCATAAGCCGATTCGAGGCGGTACAGATGGGGCATTTTTAGCCTCTAAAGGTTTAGCAT
GCCCAAATATTTTTACTGGCGGCTATAATTTCCATAGTAAACACGAGTTGGTTTCTTTACAAGGCATGGAAAATACTGTC
CAAGTAATTATCGAAATGCTGAAATGTAAAGATTTGTAA

Upstream 100 bases:

>100_bases
TGAATTTTACTTCGTTAATTTTATAAATATTGTTATATATCAAGATGTTTTCTATTTTTCTCGTTAAACTAAAAAAGATA
TTTTTAAAAGGAGCGAGAAT

Downstream 100 bases:

>100_bases
AGAAATGTAAAGATTTTTATTGCTATTCTTTAGATTACTCAATATAATGGTGGCGTTTTATCGAGATTCCTTTGATAAAG
CTAAACTTTAGAGATTTTAT

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MISQIDKTELLERFLHYVSFHTQSKPNAKHSPSSVGQMKLAMQLQKELIQFGLENVEVSKYAVVTAFLPANDPNLTKTIG
LVAHLDTSPQCSGKNVRLEVIEEYRGGDIALGIGEEFISPVYYSFMQKLVGQTLIVTDGTTLLGADNKAGIAEIMTALSI
LQKENIPHCNIRVAFTPDEEIGLGIHYFPMEKFSCDWAYTIDGGEVGELEYENFNAATAKVRFFGRNIHTGYAKGKMLNA
LTLACEFQQVFPVDEVPEKTDGKAGFYHLEDFFGDIEQVELTYLIRDFDEQNFAQRKAFIKNQVEKFNAKKGLKKPIELE
IQDSYQNMYDVVKNVPQSIELADRAMKAVGIKPNHKPIRGGTDGAFLASKGLACPNIFTGGYNFHSKHELVSLQGMENTV
QVIIEMLKCKDL

Sequences:

>Translated_412_residues
MISQIDKTELLERFLHYVSFHTQSKPNAKHSPSSVGQMKLAMQLQKELIQFGLENVEVSKYAVVTAFLPANDPNLTKTIG
LVAHLDTSPQCSGKNVRLEVIEEYRGGDIALGIGEEFISPVYYSFMQKLVGQTLIVTDGTTLLGADNKAGIAEIMTALSI
LQKENIPHCNIRVAFTPDEEIGLGIHYFPMEKFSCDWAYTIDGGEVGELEYENFNAATAKVRFFGRNIHTGYAKGKMLNA
LTLACEFQQVFPVDEVPEKTDGKAGFYHLEDFFGDIEQVELTYLIRDFDEQNFAQRKAFIKNQVEKFNAKKGLKKPIELE
IQDSYQNMYDVVKNVPQSIELADRAMKAVGIKPNHKPIRGGTDGAFLASKGLACPNIFTGGYNFHSKHELVSLQGMENTV
QVIIEMLKCKDL
>Mature_412_residues
MISQIDKTELLERFLHYVSFHTQSKPNAKHSPSSVGQMKLAMQLQKELIQFGLENVEVSKYAVVTAFLPANDPNLTKTIG
LVAHLDTSPQCSGKNVRLEVIEEYRGGDIALGIGEEFISPVYYSFMQKLVGQTLIVTDGTTLLGADNKAGIAEIMTALSI
LQKENIPHCNIRVAFTPDEEIGLGIHYFPMEKFSCDWAYTIDGGEVGELEYENFNAATAKVRFFGRNIHTGYAKGKMLNA
LTLACEFQQVFPVDEVPEKTDGKAGFYHLEDFFGDIEQVELTYLIRDFDEQNFAQRKAFIKNQVEKFNAKKGLKKPIELE
IQDSYQNMYDVVKNVPQSIELADRAMKAVGIKPNHKPIRGGTDGAFLASKGLACPNIFTGGYNFHSKHELVSLQGMENTV
QVIIEMLKCKDL

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=400, Percent_Identity=53.75, Blast_Score=440, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 46068; Mature: 46068

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISQIDKTELLERFLHYVSFHTQSKPNAKHSPSSVGQMKLAMQLQKELIQFGLENVEVSK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECE
YAVVTAFLPANDPNLTKTIGLVAHLDTSPQCSGKNVRLEVIEEYRGGDIALGIGEEFISP
EEEEEEEECCCCCCHHHHEEEEEECCCCCCCCCCCEEEEEEHHHCCCEEEEECCHHHHHH
VYYSFMQKLVGQTLIVTDGTTLLGADNKAGIAEIMTALSILQKENIPHCNIRVAFTPDEE
HHHHHHHHHHCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
IGLGIHYFPMEKFSCDWAYTIDGGEVGELEYENFNAATAKVRFFGRNIHTGYAKGKMLNA
CCCCEEEECCCCCCCCEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCHHHHH
LTLACEFQQVFPVDEVPEKTDGKAGFYHLEDFFGDIEQVELTYLIRDFDEQNFAQRKAFI
HHHEEHHHHHCCCCCCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHH
KNQVEKFNAKKGLKKPIELEIQDSYQNMYDVVKNVPQSIELADRAMKAVGIKPNHKPIRG
HHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCC
GTDGAFLASKGLACPNIFTGGYNFHSKHELVSLQGMENTVQVIIEMLKCKDL
CCCCCHHHHCCCCCCCEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISQIDKTELLERFLHYVSFHTQSKPNAKHSPSSVGQMKLAMQLQKELIQFGLENVEVSK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECE
YAVVTAFLPANDPNLTKTIGLVAHLDTSPQCSGKNVRLEVIEEYRGGDIALGIGEEFISP
EEEEEEEECCCCCCHHHHEEEEEECCCCCCCCCCCEEEEEEHHHCCCEEEEECCHHHHHH
VYYSFMQKLVGQTLIVTDGTTLLGADNKAGIAEIMTALSILQKENIPHCNIRVAFTPDEE
HHHHHHHHHHCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
IGLGIHYFPMEKFSCDWAYTIDGGEVGELEYENFNAATAKVRFFGRNIHTGYAKGKMLNA
CCCCEEEECCCCCCCCEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCHHHHH
LTLACEFQQVFPVDEVPEKTDGKAGFYHLEDFFGDIEQVELTYLIRDFDEQNFAQRKAFI
HHHEEHHHHHCCCCCCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHH
KNQVEKFNAKKGLKKPIELEIQDSYQNMYDVVKNVPQSIELADRAMKAVGIKPNHKPIRG
HHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCC
GTDGAFLASKGLACPNIFTGGYNFHSKHELVSLQGMENTVQVIIEMLKCKDL
CCCCCHHHHCCCCCCCEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA