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The map label for this gene is tmcA [H]

Identifier: 148825847

GI number: 148825847

Start: 828123

End: 830093

Strand: Direct

Name: tmcA [H]

Synonym: CGSHiEE_04030

Alternate gene names: 148825847

Gene position: 828123-830093 (Clockwise)

Preceding gene: 148825846

Following gene: 148825848

Centisome position: 45.68

GC content: 39.52

Gene sequence:

>1971_bases
ATGTCATCTCGCCAGCTTCAAATTTTAATCTGCAAAACTTTGCCGCTTGTGCCTGATAACGTATTAATTATCGGCGAAAG
TGGCATTGCTTTTTCTAAAGCAACGAATTTGCTTGGACAAGAATTTGAACATATCTTATTTGATGCGCGAAATGGCATTC
ATCTTGAAGCGCTGGCTATTGCAGCTGGAACACTGAAAATGGGCGGCACACTTTGTTTAGTGTTATCTGATTGGGAAAAT
TTATCTCAGCAGCCTGATCAAGATAGTTTACGTTGGAATGGAAATCAGTCAGCAATCGCCACGCCTAATTTTATTGATCA
TTTCAAACAGTGCATTGAACGTTATCATTTCCCCATTTTAAGAGAGGAAAGTGCGGTTGAATTTCCTACGGTTTTTTATT
CTAACGAACATCACAAAAATGCTACGTTAGCGCAACAACAAATTATCGAGACTATTTTACAAGCCGAGCAGGATATTTAT
TTTCTAACCTCCAAGCGGGGAAGAGGAAAATCCGCATTATTGGGTATGCTTGCTAACCAAATTCAAGCACCAGTTTATCT
CACCGCACCGAATAAAAGTGCGGTGCATTCCGTTATTGAATTTTCAGAACGAGAGATAGAATTTATCGCGCCTGATGAAC
TGGCTATTCGGTTACAACATGAACCAGAATTCAGTCAATCAGCTTGGTTGCTGGTAGATGAAGCGGCGGTAATCCCATTG
CCGTTATTGCAAGAATATTCTCAATATTTTCAACATATTGTCTTCAGCACAACAATTCATAGCTATGAAGGTACGGGGCG
TGGTTTCGAATTAAAATTTAAACGGAAAATTCACCGCACTTTTCAGCATTTTGAGCTTAAACAGCCCCTACGTTGGCAAG
AAAATGACCCGTTAGAACATTTTATTGATGATCTTTTGTTGCTAAATGCCGAAGATGATTTTCAGCACTTTGACTATAAC
AATATTACTTATAACATAGAGGAAAACGCTAAAAATCTTTCCTTCCCCTGCTTGCGGGGGAAGGTGCCCGAAGGGCGGAA
GGGGGATTTAGATATAGCCAGCCTCCCTCAAGCTTTGGAAGCTCCCCTCACAAGTAAGGGTAGTAAAGGAAAATATAATC
GTCAATTTTTATTTCGAGATTTTTATGGCTTAATGACATTGGCGCATTATCGAACATCGCCACTCGATCTTCGCCGTTTA
CTTGATGGAGAAAATCAACGTTTTTATTTTGCCGAATATCAACAGAATTTGCTCGGTGCAATTTGGGCATTAGAAGAAGG
AAATATGGCTGATGATGAGTTGATTATTCAAATTCAACAAGGCAAACGCCGCCCGAAAGGGAATCTTGTTCCACAGGCGT
TATGTTTCTATGAAAACTTGCCTCAAGCCTGTAAATTACGATCTTTACGTATTTCTCGTATAGCGGTTCAGCCAAATTGG
CAGCAAAAGGGCATTGGGCAAAATTTGATGCAAGCCATGGAAAATGCCGATGTGGATTTTCTTTCAGTGAGTTTTGGCTA
CACCGACGAACTGGCAAAATTTTGGCAAAAGTGCGGTTTTGTTTTGGTGCATTTAGGCGAGCATCAAGAAGCGAGTAGTG
GTTGTTATTCAGCGATTGCCCTTAAAGGACTTTCCAAAGAAGGCTTGGCTTTAGTTGATACTGCGTACAAACAATTTCAG
CGTAATCTCCCTTTATCTTTTCATCCGTTTGCCATAAATTTTGAGCAAAATCAGCTTGATTGGCAGCTTGATGATTTTGA
TTGGATGAGCTTAAAAAACTTCGCTAATTTTCACCGCACTTTATTTTCGAGCGTTCCTGCGATGAGGCGTTTATTAAAAT
TAGCGGGCAAAGAAAATTTCCCATTAATTTCCGCTTATTTAACAAAAAAACAACTTCCTATTAACAAGAAAAAAGGCGTA
GAATGTTTGCGTTTAGAAATTAAACAATATTTAGAGCGAGGAACTTTATGA

Upstream 100 bases:

>100_bases
TTTTTCAGTAAAAAAGTTTCTCAACCCAAAAGCATTTTCTTTTGGCTTGCTTGCGTAAGTTTTATTGGTGCGATGTTAAT
TGCCTATTTAAAATAAAACG

Downstream 100 bases:

>100_bases
CAGAACAAACCGCAAAACCAAAAGGCTGGTTTAAACGCGCACTTGAAAAATATGACAACTTCATTAAAGAATGGGGTTTA
GATCAACCGAATTGTAGCTG

Product: P-loop ATPase fused to an acetyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 656; Mature: 655

Protein sequence:

>656_residues
MSSRQLQILICKTLPLVPDNVLIIGESGIAFSKATNLLGQEFEHILFDARNGIHLEALAIAAGTLKMGGTLCLVLSDWEN
LSQQPDQDSLRWNGNQSAIATPNFIDHFKQCIERYHFPILREESAVEFPTVFYSNEHHKNATLAQQQIIETILQAEQDIY
FLTSKRGRGKSALLGMLANQIQAPVYLTAPNKSAVHSVIEFSEREIEFIAPDELAIRLQHEPEFSQSAWLLVDEAAVIPL
PLLQEYSQYFQHIVFSTTIHSYEGTGRGFELKFKRKIHRTFQHFELKQPLRWQENDPLEHFIDDLLLLNAEDDFQHFDYN
NITYNIEENAKNLSFPCLRGKVPEGRKGDLDIASLPQALEAPLTSKGSKGKYNRQFLFRDFYGLMTLAHYRTSPLDLRRL
LDGENQRFYFAEYQQNLLGAIWALEEGNMADDELIIQIQQGKRRPKGNLVPQALCFYENLPQACKLRSLRISRIAVQPNW
QQKGIGQNLMQAMENADVDFLSVSFGYTDELAKFWQKCGFVLVHLGEHQEASSGCYSAIALKGLSKEGLALVDTAYKQFQ
RNLPLSFHPFAINFEQNQLDWQLDDFDWMSLKNFANFHRTLFSSVPAMRRLLKLAGKENFPLISAYLTKKQLPINKKKGV
ECLRLEIKQYLERGTL

Sequences:

>Translated_656_residues
MSSRQLQILICKTLPLVPDNVLIIGESGIAFSKATNLLGQEFEHILFDARNGIHLEALAIAAGTLKMGGTLCLVLSDWEN
LSQQPDQDSLRWNGNQSAIATPNFIDHFKQCIERYHFPILREESAVEFPTVFYSNEHHKNATLAQQQIIETILQAEQDIY
FLTSKRGRGKSALLGMLANQIQAPVYLTAPNKSAVHSVIEFSEREIEFIAPDELAIRLQHEPEFSQSAWLLVDEAAVIPL
PLLQEYSQYFQHIVFSTTIHSYEGTGRGFELKFKRKIHRTFQHFELKQPLRWQENDPLEHFIDDLLLLNAEDDFQHFDYN
NITYNIEENAKNLSFPCLRGKVPEGRKGDLDIASLPQALEAPLTSKGSKGKYNRQFLFRDFYGLMTLAHYRTSPLDLRRL
LDGENQRFYFAEYQQNLLGAIWALEEGNMADDELIIQIQQGKRRPKGNLVPQALCFYENLPQACKLRSLRISRIAVQPNW
QQKGIGQNLMQAMENADVDFLSVSFGYTDELAKFWQKCGFVLVHLGEHQEASSGCYSAIALKGLSKEGLALVDTAYKQFQ
RNLPLSFHPFAINFEQNQLDWQLDDFDWMSLKNFANFHRTLFSSVPAMRRLLKLAGKENFPLISAYLTKKQLPINKKKGV
ECLRLEIKQYLERGTL
>Mature_655_residues
SSRQLQILICKTLPLVPDNVLIIGESGIAFSKATNLLGQEFEHILFDARNGIHLEALAIAAGTLKMGGTLCLVLSDWENL
SQQPDQDSLRWNGNQSAIATPNFIDHFKQCIERYHFPILREESAVEFPTVFYSNEHHKNATLAQQQIIETILQAEQDIYF
LTSKRGRGKSALLGMLANQIQAPVYLTAPNKSAVHSVIEFSEREIEFIAPDELAIRLQHEPEFSQSAWLLVDEAAVIPLP
LLQEYSQYFQHIVFSTTIHSYEGTGRGFELKFKRKIHRTFQHFELKQPLRWQENDPLEHFIDDLLLLNAEDDFQHFDYNN
ITYNIEENAKNLSFPCLRGKVPEGRKGDLDIASLPQALEAPLTSKGSKGKYNRQFLFRDFYGLMTLAHYRTSPLDLRRLL
DGENQRFYFAEYQQNLLGAIWALEEGNMADDELIIQIQQGKRRPKGNLVPQALCFYENLPQACKLRSLRISRIAVQPNWQ
QKGIGQNLMQAMENADVDFLSVSFGYTDELAKFWQKCGFVLVHLGEHQEASSGCYSAIALKGLSKEGLALVDTAYKQFQR
NLPLSFHPFAINFEQNQLDWQLDDFDWMSLKNFANFHRTLFSSVPAMRRLLKLAGKENFPLISAYLTKKQLPINKKKGVE
CLRLEIKQYLERGTL

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP) [H]

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788818, Length=599, Percent_Identity=37.5626043405676, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013562
- InterPro:   IPR007807 [H]

Pfam domain/function: PF08351 DUF1726; PF05127 DUF699 [H]

EC number: NA

Molecular weight: Translated: 75214; Mature: 75083

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRQLQILICKTLPLVPDNVLIIGESGIAFSKATNLLGQEFEHILFDARNGIHLEALAI
CCCCCEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
AAGTLKMGGTLCLVLSDWENLSQQPDQDSLRWNGNQSAIATPNFIDHFKQCIERYHFPIL
EHHHEECCCEEEEEEECHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHCCCEE
REESAVEFPTVFYSNEHHKNATLAQQQIIETILQAEQDIYFLTSKRGRGKSALLGMLANQ
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
IQAPVYLTAPNKSAVHSVIEFSEREIEFIAPDELAIRLQHEPEFSQSAWLLVDEAAVIPL
HCCCEEEECCCHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCCEEEEEECCCCCCH
PLLQEYSQYFQHIVFSTTIHSYEGTGRGFELKFKRKIHRTFQHFELKQPLRWQENDPLEH
HHHHHHHHHHHHHHHHHHEECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
FIDDLLLLNAEDDFQHFDYNNITYNIEENAKNLSFPCLRGKVPEGRKGDLDIASLPQALE
HHHHHEEECCCCCHHHCCCCCEEEEECCCCCCCCCCHHCCCCCCCCCCCCCHHHCCHHHH
APLTSKGSKGKYNRQFLFRDFYGLMTLAHYRTSPLDLRRLLDGENQRFYFAEYQQNLLGA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEHHHHHHHHHH
IWALEEGNMADDELIIQIQQGKRRPKGNLVPQALCFYENLPQACKLRSLRISRIAVQPNW
HHEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHEEEEEEEECCCH
QQKGIGQNLMQAMENADVDFLSVSFGYTDELAKFWQKCGFVLVHLGEHQEASSGCYSAIA
HHCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHH
LKGLSKEGLALVDTAYKQFQRNLPLSFHPFAINFEQNQLDWQLDDFDWMSLKNFANFHRT
HCCCCCCCCHHHHHHHHHHHHCCCCEECCEEEEEECCCCCEEECCCCHHHHHHHHHHHHH
LFSSVPAMRRLLKLAGKENFPLISAYLTKKQLPINKKKGVECLRLEIKQYLERGTL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSRQLQILICKTLPLVPDNVLIIGESGIAFSKATNLLGQEFEHILFDARNGIHLEALAI
CCCCEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
AAGTLKMGGTLCLVLSDWENLSQQPDQDSLRWNGNQSAIATPNFIDHFKQCIERYHFPIL
EHHHEECCCEEEEEEECHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHCCCEE
REESAVEFPTVFYSNEHHKNATLAQQQIIETILQAEQDIYFLTSKRGRGKSALLGMLANQ
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
IQAPVYLTAPNKSAVHSVIEFSEREIEFIAPDELAIRLQHEPEFSQSAWLLVDEAAVIPL
HCCCEEEECCCHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCCEEEEEECCCCCCH
PLLQEYSQYFQHIVFSTTIHSYEGTGRGFELKFKRKIHRTFQHFELKQPLRWQENDPLEH
HHHHHHHHHHHHHHHHHHEECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
FIDDLLLLNAEDDFQHFDYNNITYNIEENAKNLSFPCLRGKVPEGRKGDLDIASLPQALE
HHHHHEEECCCCCHHHCCCCCEEEEECCCCCCCCCCHHCCCCCCCCCCCCCHHHCCHHHH
APLTSKGSKGKYNRQFLFRDFYGLMTLAHYRTSPLDLRRLLDGENQRFYFAEYQQNLLGA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEHHHHHHHHHH
IWALEEGNMADDELIIQIQQGKRRPKGNLVPQALCFYENLPQACKLRSLRISRIAVQPNW
HHEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHEEEEEEEECCCH
QQKGIGQNLMQAMENADVDFLSVSFGYTDELAKFWQKCGFVLVHLGEHQEASSGCYSAIA
HHCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHH
LKGLSKEGLALVDTAYKQFQRNLPLSFHPFAINFEQNQLDWQLDDFDWMSLKNFANFHRT
HCCCCCCCCHHHHHHHHHHHHCCCCEECCEEEEEECCCCCEEECCCCHHHHHHHHHHHHH
LFSSVPAMRRLLKLAGKENFPLISAYLTKKQLPINKKKGVECLRLEIKQYLERGTL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]