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The map label for this gene is prfC

Identifier: 148825730

GI number: 148825730

Start: 685445

End: 686233

Strand: Direct

Name: prfC

Synonym: CGSHiEE_03375

Alternate gene names: 148825730

Gene position: 685445-686233 (Clockwise)

Preceding gene: 148825728

Following gene: 148825731

Centisome position: 37.81

GC content: 43.35

Gene sequence:

>789_bases
ATGGGTTTCTTAACAGGTAAACGCATTTTAGTCACAGGTCTTGCAAGCAATCGTTCCATCGCTTACGGGATCGCAAAATC
AATGAAAGAACAAGGTGCAGAACTTGCTTTCACTTATTTAAACGATAAATTACAACCGCGCGTAGAAGAATTTGCCAAAG
AATTTGGTTCTGATATCGTGCTTCCTTTAGATGTGGCAACCGATGAAAGCATCCAAAACTGCTTTGCAGAATTAAGTAAG
CGTTGGGATAAATTTGATGGTTTTATTCACGCAATCGCATTTGCACCAGGTGATCAATTAGACGGCGATTACGTAAATGC
GGCAACTCGTGAAGGCTATCGTATCGCTCATGATATTAGCGCATACAGCTTTGTTGCAATGGCACAAGCGGCACGTCCTT
ACTTAAATCCAAATGCCGCATTATTAACGCTTTCTTATTTAGGTGCAGAACGTGCAATTCCTAACTACAACGTAATGTGC
CTAGCAAAAGCCTCTCTTGAAGCTGCAACGCGCGTGATGGCTGCTGATTTAGGGAAAGAAGGTATTCGTGTGAATGCAAT
CTCTGCAGGCCCAATCCGCACCTTAGCCGCATCAGGCATTAAAAACTTCAAAAAAATGCTTTCCACCTTTGAGAAAACGG
CTGCATTACGTCGCACTGTCACTATCGAAGATGTGGGTAACTCAGCGGCATTTTTATGCTCTGATTTAGCATCGGGCATT
ACAGGCGAAATCGTTCACGTTGATGCAGGTTTCAGCATCACTGCAATGGGCGAATTAGGCGAAGAATAA

Upstream 100 bases:

>100_bases
AGTGCGGTTGGTTTTCTTAGAAATATTGGTCTGCTTTGTACAAAGACAAATTTTCGCTTATACTACGCAAGTTTTTTACG
CTAACAACATAGGAAAAATT

Downstream 100 bases:

>100_bases
TTTTTCTTACATTCTTTTTTAGGCAGGCTTTTCAGTCTGCCTTTTCGCTTTTTTAAAATATAACGATGTTTCAAAATAAC
TATGTTTCAAGATAATTATG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MGFLTGKRILVTGLASNRSIAYGIAKSMKEQGAELAFTYLNDKLQPRVEEFAKEFGSDIVLPLDVATDESIQNCFAELSK
RWDKFDGFIHAIAFAPGDQLDGDYVNAATREGYRIAHDISAYSFVAMAQAARPYLNPNAALLTLSYLGAERAIPNYNVMC
LAKASLEAATRVMAADLGKEGIRVNAISAGPIRTLAASGIKNFKKMLSTFEKTAALRRTVTIEDVGNSAAFLCSDLASGI
TGEIVHVDAGFSITAMGELGEE

Sequences:

>Translated_262_residues
MGFLTGKRILVTGLASNRSIAYGIAKSMKEQGAELAFTYLNDKLQPRVEEFAKEFGSDIVLPLDVATDESIQNCFAELSK
RWDKFDGFIHAIAFAPGDQLDGDYVNAATREGYRIAHDISAYSFVAMAQAARPYLNPNAALLTLSYLGAERAIPNYNVMC
LAKASLEAATRVMAADLGKEGIRVNAISAGPIRTLAASGIKNFKKMLSTFEKTAALRRTVTIEDVGNSAAFLCSDLASGI
TGEIVHVDAGFSITAMGELGEE
>Mature_261_residues
GFLTGKRILVTGLASNRSIAYGIAKSMKEQGAELAFTYLNDKLQPRVEEFAKEFGSDIVLPLDVATDESIQNCFAELSKR
WDKFDGFIHAIAFAPGDQLDGDYVNAATREGYRIAHDISAYSFVAMAQAARPYLNPNAALLTLSYLGAERAIPNYNVMCL
AKASLEAATRVMAADLGKEGIRVNAISAGPIRTLAASGIKNFKKMLSTFEKTAALRRTVTIEDVGNSAAFLCSDLASGIT
GEIVHVDAGFSITAMGELGEE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily

Homologues:

Organism=Homo sapiens, GI4503301, Length=237, Percent_Identity=28.6919831223629, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=75.2895752895753, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1787905, Length=140, Percent_Identity=32.1428571428571, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI87082100, Length=263, Percent_Identity=27.7566539923954, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=24.901185770751, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=259, Percent_Identity=27.4131274131274, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644337, Length=120, Percent_Identity=35, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): FABI_HAEIN (P44432)

Other databases:

- EMBL:   L42023
- PIR:   B64139
- RefSeq:   NP_439876.2
- ProteinModelPortal:   P44432
- SMR:   P44432
- GeneID:   950535
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1734
- TIGR:   HI_1734
- HOGENOM:   HBG750976
- OMA:   LVHCLAF
- ProtClustDB:   CLSK918312
- BioCyc:   HINF71421:HI_1734-MONOMER
- BRENDA:   1.3.1.9
- BindingDB:   P44432
- GO:   GO:0005488
- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PANTHER:   PTHR19410:SF12
- PIRSF:   PIRSF000094
- PRINTS:   PR00081

Pfam domain/function: PF00106 adh_short

EC number: =1.3.1.9

Molecular weight: Translated: 28119; Mature: 27988

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: ACT_SITE 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLTGKRILVTGLASNRSIAYGIAKSMKEQGAELAFTYLNDKLQPRVEEFAKEFGSDIV
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHEEEEHHCCCCCHHHHHHHHHHCCCEE
LPLDVATDESIQNCFAELSKRWDKFDGFIHAIAFAPGDQLDGDYVNAATREGYRIAHDIS
EEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHCCCEEEEHHHH
AYSFVAMAQAARPYLNPNAALLTLSYLGAERAIPNYNVMCLAKASLEAATRVMAADLGKE
HHHHHHHHHHHCCCCCCCHHEEEEEHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCC
GIRVNAISAGPIRTLAASGIKNFKKMLSTFEKTAALRRTVTIEDVGNSAAFLCSDLASGI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHCCC
TGEIVHVDAGFSITAMGELGEE
CCEEEEEECCCEEEECCCCCCC
>Mature Secondary Structure 
GFLTGKRILVTGLASNRSIAYGIAKSMKEQGAELAFTYLNDKLQPRVEEFAKEFGSDIV
CCCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHEEEEHHCCCCCHHHHHHHHHHCCCEE
LPLDVATDESIQNCFAELSKRWDKFDGFIHAIAFAPGDQLDGDYVNAATREGYRIAHDIS
EEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHCCCEEEEHHHH
AYSFVAMAQAARPYLNPNAALLTLSYLGAERAIPNYNVMCLAKASLEAATRVMAADLGKE
HHHHHHHHHHHCCCCCCCHHEEEEEHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCC
GIRVNAISAGPIRTLAASGIKNFKKMLSTFEKTAALRRTVTIEDVGNSAAFLCSDLASGI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHCCC
TGEIVHVDAGFSITAMGELGEE
CCEEEEEECCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800