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The map label for this gene is citF [H]

Identifier: 148825697

GI number: 148825697

Start: 641536

End: 643038

Strand: Direct

Name: citF [H]

Synonym: CGSHiEE_03200

Alternate gene names: 148825697

Gene position: 641536-643038 (Clockwise)

Preceding gene: 148825696

Following gene: 148825698

Centisome position: 35.38

GC content: 41.92

Gene sequence:

>1503_bases
ATGACAACAAGAGAACAACGTATTGAAAAATATCACGCTGATCGCTCAGTCTATCAAGCGGTGCCTAAATCTGAATCCCT
TTCTCGTACAGCGAAAGATCGCAAACTTTGTACCTCTCTAGAAGAAGCGATTAAACGCTCTGGCTTAAAAGATGGTATGA
CTGTTTCATTTCATCATGCTTTTCGGGGCGGAGATTTTGTGGTGAATATGGTGATGAATAAAATTGCCGAAATGGGTTTC
AAAAATCTGACCCTTGCATCTTCATCCTTAATTGATAGCCACTTCCCGATTGTCGAACATATCAAAAATGGCGTGGTCAC
CAAAATCTATTCTTCTGGTTTACGCGGTGAACTTGCAGAGCAAATCTCTCGCGGTTTATTGAATGAACCTGTAAATATTC
ATTCTCATGGCGGACGTGTTCACTTAGTAAAATCTGGTGAATTAAAAATTGACGTTGCTTTTTTAGGCGTACCTTGCTGT
GACACATTTGGTAATTCCAATGGATTTACTGGAAAAAGTAAATGCGGCTCTCTAGGCTATGCGCGTGTCGATGCTGAATA
CGCTGACAAAGTTGTGTTGCTCACAGAGGAATTTGCTGAATACCCGCACCACCCAATCGGCATTGCTCAAGATCAAGTAA
ATTTAATCGTGCAAGTTGAAGCCGTCGGTGACCCGAAAAAAATTGGCGGTGGCGCAACCCGAATGACTACCAATCCACGC
GAATTACTCATCGCGCGTAAATGTGCAGAAGTAATTTTTGCCAGTGGTTATTTTAAAGATGGCTTCTCATTACAAACTGG
TTCTGGGGGGGCTGCATTAGCTGTTACACGCTTCCTAGAAGAAAAAATGCGTCGTGAAAATATCACTGCCGATTTCGCAC
TTGGCGGTATTACCGCAAGTATGGTGGCATTACATGAGGCTGGTCTTATTAAAAAATTATTAGATGTTCAAAGTTTTGAC
TCTGTTGCGGCTGAATCACTTGCTCGCAACCCAAATCATATTGAAGTTTCCGCTAACCAATATGCGAACTATAGTTCCAA
AGGAGCATCGGTTGAACGTTTAGATATGGTTATTCTTTCTGCTCTAGAAATCGACACTAAATTTAACGTGAATGTATTGA
CTGGCTCTGACGGTGTGATTCGCGGTGCATCTGGTGGTCATTGCGATACCGCAGCTTCTGCACAAATAGCAATCATTGTG
GCACCATTAGTTCGTGGTCGTATTCCAACCGTGGTGGAAAACGTGATTACCTGTGTAACACCTGGTGAAAACGTCGATAT
TCTCGTTACCGATCATGGTGTAGCAGTAAATCCTAAACGTCCAGATTTGATAGACGCTTTAAATAAAGCAGATATTCCTT
TGTTTACCATTGAACAGCTTTGTGAGCGCGCTTACAGCATTACAGGTAAACCAAAAGAAATTGAGTTTACTAATAAACCT
GTGGCTGTTGTTCGTTATCGTGATGGTTCGGTGATTGATACGGTGTATCAAGTTAAAGACTAG

Upstream 100 bases:

>100_bases
TAAACGGTAAAATGATTGACGCACCGATTATTGATCGCGCAAAACTTGTATTAGAACGTGCGAAATCAGGTATTCGTGAA
GAGTAAAGGGAGATAAAAAG

Downstream 100 bases:

>100_bases
AATCAGCATTACAAGCGATCTACTTTCAATAGCCAAAATTAACTCTTGTCTTCCCATGATTATCAGGAAAGAAACCCAAA
GAGTTGAAAGGGACTCAATT

Product: citrate lyase subunit alpha

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MTTREQRIEKYHADRSVYQAVPKSESLSRTAKDRKLCTSLEEAIKRSGLKDGMTVSFHHAFRGGDFVVNMVMNKIAEMGF
KNLTLASSSLIDSHFPIVEHIKNGVVTKIYSSGLRGELAEQISRGLLNEPVNIHSHGGRVHLVKSGELKIDVAFLGVPCC
DTFGNSNGFTGKSKCGSLGYARVDAEYADKVVLLTEEFAEYPHHPIGIAQDQVNLIVQVEAVGDPKKIGGGATRMTTNPR
ELLIARKCAEVIFASGYFKDGFSLQTGSGGAALAVTRFLEEKMRRENITADFALGGITASMVALHEAGLIKKLLDVQSFD
SVAAESLARNPNHIEVSANQYANYSSKGASVERLDMVILSALEIDTKFNVNVLTGSDGVIRGASGGHCDTAASAQIAIIV
APLVRGRIPTVVENVITCVTPGENVDILVTDHGVAVNPKRPDLIDALNKADIPLFTIEQLCERAYSITGKPKEIEFTNKP
VAVVRYRDGSVIDTVYQVKD

Sequences:

>Translated_500_residues
MTTREQRIEKYHADRSVYQAVPKSESLSRTAKDRKLCTSLEEAIKRSGLKDGMTVSFHHAFRGGDFVVNMVMNKIAEMGF
KNLTLASSSLIDSHFPIVEHIKNGVVTKIYSSGLRGELAEQISRGLLNEPVNIHSHGGRVHLVKSGELKIDVAFLGVPCC
DTFGNSNGFTGKSKCGSLGYARVDAEYADKVVLLTEEFAEYPHHPIGIAQDQVNLIVQVEAVGDPKKIGGGATRMTTNPR
ELLIARKCAEVIFASGYFKDGFSLQTGSGGAALAVTRFLEEKMRRENITADFALGGITASMVALHEAGLIKKLLDVQSFD
SVAAESLARNPNHIEVSANQYANYSSKGASVERLDMVILSALEIDTKFNVNVLTGSDGVIRGASGGHCDTAASAQIAIIV
APLVRGRIPTVVENVITCVTPGENVDILVTDHGVAVNPKRPDLIDALNKADIPLFTIEQLCERAYSITGKPKEIEFTNKP
VAVVRYRDGSVIDTVYQVKD
>Mature_499_residues
TTREQRIEKYHADRSVYQAVPKSESLSRTAKDRKLCTSLEEAIKRSGLKDGMTVSFHHAFRGGDFVVNMVMNKIAEMGFK
NLTLASSSLIDSHFPIVEHIKNGVVTKIYSSGLRGELAEQISRGLLNEPVNIHSHGGRVHLVKSGELKIDVAFLGVPCCD
TFGNSNGFTGKSKCGSLGYARVDAEYADKVVLLTEEFAEYPHHPIGIAQDQVNLIVQVEAVGDPKKIGGGATRMTTNPRE
LLIARKCAEVIFASGYFKDGFSLQTGSGGAALAVTRFLEEKMRRENITADFALGGITASMVALHEAGLIKKLLDVQSFDS
VAAESLARNPNHIEVSANQYANYSSKGASVERLDMVILSALEIDTKFNVNVLTGSDGVIRGASGGHCDTAASAQIAIIVA
PLVRGRIPTVVENVITCVTPGENVDILVTDHGVAVNPKRPDLIDALNKADIPLFTIEQLCERAYSITGKPKEIEFTNKPV
AVVRYRDGSVIDTVYQVKD

Specific function: Represents a citrate:acetyl-ACP transferase [H]

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=503, Percent_Identity=71.3717693836978, Blast_Score=750, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006472 [H]

Pfam domain/function: PF04223 CitF [H]

EC number: =4.1.3.6; =2.8.3.10 [H]

Molecular weight: Translated: 54088; Mature: 53957

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTREQRIEKYHADRSVYQAVPKSESLSRTAKDRKLCTSLEEAIKRSGLKDGMTVSFHHA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
FRGGDFVVNMVMNKIAEMGFKNLTLASSSLIDSHFPIVEHIKNGVVTKIYSSGLRGELAE
CCCCHHHHHHHHHHHHHCCCCCEEECHHHHHHCCCHHHHHHHCCHHHHHHHCCCCHHHHH
QISRGLLNEPVNIHSHGGRVHLVKSGELKIDVAFLGVPCCDTFGNSNGFTGKSKCGSLGY
HHHHHHHCCCCEEECCCCEEEEEECCCEEEEEEEEECCCHHHCCCCCCCCCCCCCCCCCE
ARVDAEYADKVVLLTEEFAEYPHHPIGIAQDQVNLIVQVEAVGDPKKIGGGATRMTTNPR
EEECHHHCCEEEEEEHHHHHCCCCCCCCCCCCEEEEEEEEECCCHHHCCCCCCEECCCHH
ELLIARKCAEVIFASGYFKDGFSLQTGSGGAALAVTRFLEEKMRRENITADFALGGITAS
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
MVALHEAGLIKKLLDVQSFDSVAAESLARNPNHIEVSANQYANYSSKGASVERLDMVILS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCCCCCCCHHHHHHHHHH
ALEIDTKFNVNVLTGSDGVIRGASGGHCDTAASAQIAIIVAPLVRGRIPTVVENVITCVT
HHEECCEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEEEEHHHCCCCHHHHHHHEEEEC
PGENVDILVTDHGVAVNPKRPDLIDALNKADIPLFTIEQLCERAYSITGKPKEIEFTNKP
CCCCEEEEEECCCEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCC
VAVVRYRDGSVIDTVYQVKD
EEEEEECCCCEEEHEEECCC
>Mature Secondary Structure 
TTREQRIEKYHADRSVYQAVPKSESLSRTAKDRKLCTSLEEAIKRSGLKDGMTVSFHHA
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
FRGGDFVVNMVMNKIAEMGFKNLTLASSSLIDSHFPIVEHIKNGVVTKIYSSGLRGELAE
CCCCHHHHHHHHHHHHHCCCCCEEECHHHHHHCCCHHHHHHHCCHHHHHHHCCCCHHHHH
QISRGLLNEPVNIHSHGGRVHLVKSGELKIDVAFLGVPCCDTFGNSNGFTGKSKCGSLGY
HHHHHHHCCCCEEECCCCEEEEEECCCEEEEEEEEECCCHHHCCCCCCCCCCCCCCCCCE
ARVDAEYADKVVLLTEEFAEYPHHPIGIAQDQVNLIVQVEAVGDPKKIGGGATRMTTNPR
EEECHHHCCEEEEEEHHHHHCCCCCCCCCCCCEEEEEEEEECCCHHHCCCCCCEECCCHH
ELLIARKCAEVIFASGYFKDGFSLQTGSGGAALAVTRFLEEKMRRENITADFALGGITAS
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
MVALHEAGLIKKLLDVQSFDSVAAESLARNPNHIEVSANQYANYSSKGASVERLDMVILS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCCCCCCCHHHHHHHHHH
ALEIDTKFNVNVLTGSDGVIRGASGGHCDTAASAQIAIIVAPLVRGRIPTVVENVITCVT
HHEECCEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEEEEHHHCCCCHHHHHHHEEEEC
PGENVDILVTDHGVAVNPKRPDLIDALNKADIPLFTIEQLCERAYSITGKPKEIEFTNKP
CCCCEEEEEECCCEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCC
VAVVRYRDGSVIDTVYQVKD
EEEEEECCCCEEEHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]