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The map label for this gene is metB [H]

Identifier: 148825637

GI number: 148825637

Start: 576581

End: 577690

Strand: Direct

Name: metB [H]

Synonym: CGSHiEE_02855

Alternate gene names: 148825637

Gene position: 576581-577690 (Clockwise)

Preceding gene: 148825636

Following gene: 148825638

Centisome position: 31.8

GC content: 38.2

Gene sequence:

>1110_bases
ATGACACAACAATATGCTATTGATACTTTATTAGCTCAAGCAGGCAATCGCAGTGATGAGCGTACGGGCGCGGTTTCTGC
ACCAATTTTTCTTTCTACGGCTTATGGTCATTGTGGTATCGGTGAAAGTACGGGATTTGATTACACACGTACCAAAAATC
CAACTCGCACAGTATTAGAAGAAACGATAGCTAAATTAGAGAATGGAGATCGTGGCTTTGCGTTTTCTTCTGGTATGGCT
GCAATTCAAGTTTTGATGACGCTTTTTACCGCCCTAGATGAATGGATTGTTTCTAGCGATGTGTATGGTGGTACTTATCG
ACTATTAGATTTTTCTTATAAAAATAACAATAGTGTAAAACCTGTTTATGTTAATACAGCATCTGCTTCAGAAATTGAGG
CTGCCATTAACCCAAATACGAAAGCGATTTTTATTGAAACCCCATCGAATCCTTTAATGGAAGAATGTGATGTGGTTGAA
ATTGCAAAACTTGCGAAAAAACATAATTTGATGTTGATCGTGGATAATACGTTTCTAACGCCAGTGCTTTCTCGCCCATT
AGATTTAGGTGCGGATGTGGTAATTCACAGCGGGACTAAATATATTGCTGGTCATAATGATGCACTTGTTGGTTTAATCG
TTGCAAAAGGGCAAGAACTTTGTGATCGCATTGCTTATATTCAAAATGGTGCAGGTGCGGTGCTTTCTCCTTTTGATTCT
TGGCTGACTATTCGCGGTATGAAGACACTTTCTTTGCGTATGAAACGCCATCAAGAAAATGCACAAGCTATTGCGGAATT
TTTAAAAGATCAACCACAAGTGGAATCGGTGCTTTATCCAAATAAAGGTGGAATGTTGTCTTTCCGTTTACAAGATGAAG
CTTGGGTTAATACATTCTTAAAATCCATTAAATTGATTACTTTTGCAGAAAGTCTAGGCGGCACAGAAAGTTTTATTACT
TATCCTGCCACTCAAACTCATATGGATATTCCAGAAACCGAGCGTGTAGCTCGTGGGATTACTAATACCTTGTTGCGTTT
TTCAGTCGGTATTGAAGATGTAGAAGATATTAAAGCGGACTTATTACAAGCTTTTGCAAACTTAAAATAA

Upstream 100 bases:

>100_bases
CTAATTTCTACTTTTATCACTTTTTCCTTTGTGCCAAAATGCTAAACGTTTTTTAACACGTAAAGTGCGGTCAATTTTTT
CATTATTTTTAGAGGATGTT

Downstream 100 bases:

>100_bases
TTGGAGCAAACCATGAAAATTGCAATCTATAGCACTAAAAGTTATGACAGAAAATATATTGAGCTCATTAATGCGAAATA
TAATTTCGATTTAGAATTTT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTQQYAIDTLLAQAGNRSDERTGAVSAPIFLSTAYGHCGIGESTGFDYTRTKNPTRTVLEETIAKLENGDRGFAFSSGMA
AIQVLMTLFTALDEWIVSSDVYGGTYRLLDFSYKNNNSVKPVYVNTASASEIEAAINPNTKAIFIETPSNPLMEECDVVE
IAKLAKKHNLMLIVDNTFLTPVLSRPLDLGADVVIHSGTKYIAGHNDALVGLIVAKGQELCDRIAYIQNGAGAVLSPFDS
WLTIRGMKTLSLRMKRHQENAQAIAEFLKDQPQVESVLYPNKGGMLSFRLQDEAWVNTFLKSIKLITFAESLGGTESFIT
YPATQTHMDIPETERVARGITNTLLRFSVGIEDVEDIKADLLQAFANLK

Sequences:

>Translated_369_residues
MTQQYAIDTLLAQAGNRSDERTGAVSAPIFLSTAYGHCGIGESTGFDYTRTKNPTRTVLEETIAKLENGDRGFAFSSGMA
AIQVLMTLFTALDEWIVSSDVYGGTYRLLDFSYKNNNSVKPVYVNTASASEIEAAINPNTKAIFIETPSNPLMEECDVVE
IAKLAKKHNLMLIVDNTFLTPVLSRPLDLGADVVIHSGTKYIAGHNDALVGLIVAKGQELCDRIAYIQNGAGAVLSPFDS
WLTIRGMKTLSLRMKRHQENAQAIAEFLKDQPQVESVLYPNKGGMLSFRLQDEAWVNTFLKSIKLITFAESLGGTESFIT
YPATQTHMDIPETERVARGITNTLLRFSVGIEDVEDIKADLLQAFANLK
>Mature_368_residues
TQQYAIDTLLAQAGNRSDERTGAVSAPIFLSTAYGHCGIGESTGFDYTRTKNPTRTVLEETIAKLENGDRGFAFSSGMAA
IQVLMTLFTALDEWIVSSDVYGGTYRLLDFSYKNNNSVKPVYVNTASASEIEAAINPNTKAIFIETPSNPLMEECDVVEI
AKLAKKHNLMLIVDNTFLTPVLSRPLDLGADVVIHSGTKYIAGHNDALVGLIVAKGQELCDRIAYIQNGAGAVLSPFDSW
LTIRGMKTLSLRMKRHQENAQAIAEFLKDQPQVESVLYPNKGGMLSFRLQDEAWVNTFLKSIKLITFAESLGGTESFITY
PATQTHMDIPETERVARGITNTLLRFSVGIEDVEDIKADLLQAFANLK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=345, Percent_Identity=40.2898550724638, Blast_Score=262, Evalue=3e-70,
Organism=Homo sapiens, GI299473759, Length=345, Percent_Identity=36.5217391304348, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI25453487, Length=344, Percent_Identity=35.4651162790698, Blast_Score=206, Evalue=3e-53,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=39.686684073107, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI1789383, Length=399, Percent_Identity=29.5739348370927, Blast_Score=137, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17538055, Length=347, Percent_Identity=42.0749279538905, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17560056, Length=347, Percent_Identity=42.0749279538905, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI71980708, Length=395, Percent_Identity=33.9240506329114, Blast_Score=200, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6319307, Length=378, Percent_Identity=39.9470899470899, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6321254, Length=403, Percent_Identity=30.7692307692308, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=31.9444444444444, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6321170, Length=282, Percent_Identity=31.5602836879433, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=386, Percent_Identity=25.6476683937824, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922554, Length=373, Percent_Identity=39.6782841823056, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 40504; Mature: 40372

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQYAIDTLLAQAGNRSDERTGAVSAPIFLSTAYGHCGIGESTGFDYTRTKNPTRTVLE
CCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
ETIAKLENGDRGFAFSSGMAAIQVLMTLFTALDEWIVSSDVYGGTYRLLDFSYKNNNSVK
HHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCC
PVYVNTASASEIEAAINPNTKAIFIETPSNPLMEECDVVEIAKLAKKHNLMLIVDNTFLT
EEEEECCCCHHHHEEECCCCCEEEEECCCCHHHHHCCHHHHHHHHHHCCEEEEECCCHHH
PVLSRPLDLGADVVIHSGTKYIAGHNDALVGLIVAKGQELCDRIAYIQNGAGAVLSPFDS
HHHCCCCCCCCCEEEECCCEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCEECCCCC
WLTIRGMKTLSLRMKRHQENAQAIAEFLKDQPQVESVLYPNKGGMLSFRLQDEAWVNTFL
CEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCEEEEEECHHHHHHHHH
KSIKLITFAESLGGTESFITYPATQTHMDIPETERVARGITNTLLRFSVGIEDVEDIKAD
HHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LLQAFANLK
HHHHHHCCC
>Mature Secondary Structure 
TQQYAIDTLLAQAGNRSDERTGAVSAPIFLSTAYGHCGIGESTGFDYTRTKNPTRTVLE
CCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
ETIAKLENGDRGFAFSSGMAAIQVLMTLFTALDEWIVSSDVYGGTYRLLDFSYKNNNSVK
HHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCC
PVYVNTASASEIEAAINPNTKAIFIETPSNPLMEECDVVEIAKLAKKHNLMLIVDNTFLT
EEEEECCCCHHHHEEECCCCCEEEEECCCCHHHHHCCHHHHHHHHHHCCEEEEECCCHHH
PVLSRPLDLGADVVIHSGTKYIAGHNDALVGLIVAKGQELCDRIAYIQNGAGAVLSPFDS
HHHCCCCCCCCCEEEECCCEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCEECCCCC
WLTIRGMKTLSLRMKRHQENAQAIAEFLKDQPQVESVLYPNKGGMLSFRLQDEAWVNTFL
CEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCEEEEEECHHHHHHHHH
KSIKLITFAESLGGTESFITYPATQTHMDIPETERVARGITNTLLRFSVGIEDVEDIKAD
HHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LLQAFANLK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]