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The map label for this gene is yaiM [C]

Identifier: 148825551

GI number: 148825551

Start: 459193

End: 460020

Strand: Direct

Name: yaiM [C]

Synonym: CGSHiEE_02340

Alternate gene names: 148825551

Gene position: 459193-460020 (Clockwise)

Preceding gene: 148825550

Following gene: 148825552

Centisome position: 25.33

GC content: 44.32

Gene sequence:

>828_bases
ATGAAACTAATTGAACAACATCAAATTTTTGGCGGTTCACAGCAAGTTTGGGCACATAATGCCCAAACGCTTCAATGCGA
AATGAAATTTGCCGTCTATTTGCCAAATAATCCAGAAAATCGACCGCTTGGCGTGATTTATTGGCTTTCTGGCTTAACTT
GTACAGAACAAAATTTCATTACCAAATCAGGCTTCCAGCGTTATGCGGCAGAACATCAAGTGATTGTGGTTGCGCCTGAT
ACTAGCCCTCGTGGAGAGCAAGTGCCGAACGATGCGGTTTACGATTTAGGGCAGGGAGCGGGCTTTTATCTTAATGCGAC
CGAGCAGCCTTGGGCGACGAATTATCAAATGTATGATTATATCTTGAATGAATTGCCTGATTTGATTGAAGCAAATTTCC
CTACCAACGGCAAACGTTCCATTATGGGACATTCAATGGGCGGACACGGTGCATTGGTATTGGCACTGCGAAATCGGGAA
CGTTATCAAAGTGTTTCTGCCTTTTCGCCCATTTTGTCGCCAAGCCAGGTGCCTTGGGGAGAAAAAGCCTTTACTGCCTA
TTTAGGGGAAGATCGTGAAAAATGGCAGCAATATGATGCCAGCTCGCTCATTCAACAAGGCTATAAAGTGCAAGGTATAC
GCATTGATCAGGGCTTGGAAGATGAGTTTTTGCCTACACAATTGCGTACCGAAGATTTTATCGAAACCTGTCGTGCAGCA
AATCAGCCAGTCGATGTGCGCTTCCATAAAGGCTATGATCACAGCTATTACTTCATCGCCAGTTTTATTGGCGAGCATAT
TGCCTATCATGCGGCATTTTTGAAGTAA

Upstream 100 bases:

>100_bases
TTGTGACTCACACAATGCCACTTGATCAAATTAATGAAGCCTTTGAATTAATGCACGAAGGTAAATCAATCCGTACTGTT
ATTCACTACTAAGGTACGCT

Downstream 100 bases:

>100_bases
GCTAAAGAGCGGTCAGTTTTCAAAGCAGTTTGGGATAGCCGGCACGAGGGCGGTAAAAAGTGCCGGCAAAATGCCGATAT
AAACGTATGCCATCTGAACT

Product: esterase

Products: NA

Alternate protein names: FGH [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLGVIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPD
TSPRGEQVPNDAVYDLGQGAGFYLNATEQPWATNYQMYDYILNELPDLIEANFPTNGKRSIMGHSMGGHGALVLALRNRE
RYQSVSAFSPILSPSQVPWGEKAFTAYLGEDREKWQQYDASSLIQQGYKVQGIRIDQGLEDEFLPTQLRTEDFIETCRAA
NQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK

Sequences:

>Translated_275_residues
MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLGVIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPD
TSPRGEQVPNDAVYDLGQGAGFYLNATEQPWATNYQMYDYILNELPDLIEANFPTNGKRSIMGHSMGGHGALVLALRNRE
RYQSVSAFSPILSPSQVPWGEKAFTAYLGEDREKWQQYDASSLIQQGYKVQGIRIDQGLEDEFLPTQLRTEDFIETCRAA
NQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK
>Mature_275_residues
MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLGVIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPD
TSPRGEQVPNDAVYDLGQGAGFYLNATEQPWATNYQMYDYILNELPDLIEANFPTNGKRSIMGHSMGGHGALVLALRNRE
RYQSVSAFSPILSPSQVPWGEKAFTAYLGEDREKWQQYDASSLIQQGYKVQGIRIDQGLEDEFLPTQLRTEDFIETCRAA
NQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK

Specific function: Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate [H]

COG id: COG0627

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the esterase D family [H]

Homologues:

Organism=Homo sapiens, GI33413400, Length=278, Percent_Identity=54.3165467625899, Blast_Score=314, Evalue=6e-86,
Organism=Escherichia coli, GI1786551, Length=271, Percent_Identity=63.4686346863469, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1788477, Length=276, Percent_Identity=52.536231884058, Blast_Score=309, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17510185, Length=278, Percent_Identity=48.5611510791367, Blast_Score=266, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6322393, Length=293, Percent_Identity=41.296928327645, Blast_Score=228, Evalue=5e-61,
Organism=Drosophila melanogaster, GI45551932, Length=281, Percent_Identity=49.4661921708185, Blast_Score=279, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24648347, Length=281, Percent_Identity=49.4661921708185, Blast_Score=279, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000801
- InterPro:   IPR014186 [H]

Pfam domain/function: PF00756 Esterase [H]

EC number: =3.1.2.12 [H]

Molecular weight: Translated: 31270; Mature: 31270

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLGVIYWLSGLTCTEQNFI
CCCCHHHHHCCCCCCEEECCCEEEEEEEEEEEECCCCCCCCCEEEEEEECCCEECCCCHH
TKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDAVYDLGQGAGFYLNATEQPWATNYQMYDY
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCCCHHHHHH
ILNELPDLIEANFPTNGKRSIMGHSMGGHGALVLALRNRERYQSVSAFSPILSPSQVPWG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCC
EKAFTAYLGEDREKWQQYDASSLIQQGYKVQGIRIDQGLEDEFLPTQLRTEDFIETCRAA
CHHHHHHHCCCHHHHHHCCHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHC
NQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLGVIYWLSGLTCTEQNFI
CCCCHHHHHCCCCCCEEECCCEEEEEEEEEEEECCCCCCCCCEEEEEEECCCEECCCCHH
TKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDAVYDLGQGAGFYLNATEQPWATNYQMYDY
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCCCHHHHHH
ILNELPDLIEANFPTNGKRSIMGHSMGGHGALVLALRNRERYQSVSAFSPILSPSQVPWG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCC
EKAFTAYLGEDREKWQQYDASSLIQQGYKVQGIRIDQGLEDEFLPTQLRTEDFIETCRAA
CHHHHHHHCCCHHHHHHCCHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHC
NQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]