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The map label for this gene is frmA [H]

Identifier: 148825550

GI number: 148825550

Start: 458048

End: 459184

Strand: Direct

Name: frmA [H]

Synonym: CGSHiEE_02335

Alternate gene names: 148825550

Gene position: 458048-459184 (Clockwise)

Preceding gene: 148825544

Following gene: 148825551

Centisome position: 25.26

GC content: 45.65

Gene sequence:

>1137_bases
ATGGAAATTAAACAAATCAACTCAATCATCAAATCTCGTGCGGCGGTAGCATTCGCCCCAAATCAACCCTTACAAATTGT
AGAAATCGACGTAGAAATGCCTCGCAAAGGAGAAGTGTTAATTCGTAATACGCACACAGGCGTTTGTCATACTGATGCAT
TTACGTTATCAGGAAGCGATCCTGAAGGGGTATTCCCTGTGGTGCTAGGACACGAAGGTGCGGGTGTGGTTGTTGCGGTT
GGCGAAGGTGTGTTAAGCGTAAAACCGGGCGATCACGTCATTCCTCTTTATACTGCTGAATGTGGCGAATGTGAGTTTTG
CCGCTCGGGTAAAACCAACTTATGCGTCTCAGTGCGTGACACGCAAGGTAAAGGCTTAATGCCTGATTGCACGACACGTT
TTTCTTATCAAGGTCAGCCGATCTATCACTATATGGGCTGTTCAACCTTCAGTGAATACTCAGTTGTTGCAGAAGTTTCA
CTGGCGAAAATCAACCCAGAAGCCAACCACGAACAAGTGTGTTTGCTCGGCTGCGGTGTCACCACAGGTATTGGTGCGGT
ACATAATACGGCAAAAGTGCAAGAAGGCGACTCTGTTGCCGTGTTTGGCTTGGGTGCGATTGGTTTAGCGGTAGTGCAAG
GTGCGCGTCAAGCCAAAGCAGGTCGCATTATCGCCATTGATACCAATCCTGCAAAATTTGAGTTGGCAAAACAGTTTGGT
GCAACGGATTGTTTAAACCCTAATGATTACGATAAGCCGATCAAAGATGTGTTGTTAGATATTAATAAATGGGGCATTGA
CCATACCTTTGAATGTATCGGTAATGTAAACGTAATGCGTCAAGCATTAGAAAGTGCTCACCGTGGCTGGGGACAATCCA
TTATCATCGGCGTAGCGGGTGCAGGACAAGAAATTTCAACGCGTCCGTTCCAATTGGTAACAGGCCGTGTTTGGAAAGGT
TCGGCATTTGGTGGCGTGAAAGGTCGCTCTGAACTTCCGCAAATGGTGGAAGATTCAATGAAAGGCGACATCCAGTTAGA
ACCGTTTGTGACTCACACAATGCCACTTGATCAAATTAATGAAGCCTTTGAATTAATGCACGAAGGTAAATCAATCCGTA
CTGTTATTCACTACTAA

Upstream 100 bases:

>100_bases
AATGATAAAAAACACTTGCTTTAGAGTGAACTCTAAAGTGTAAACTGTTGCTATCTTGCTTAGGCAAGGCAAATTTGTCA
ATGAATTAAGAGGAAAAACA

Downstream 100 bases:

>100_bases
GGTACGCTATGAAACTAATTGAACAACATCAAATTTTTGGCGGTTCACAGCAAGTTTGGGCACATAATGCCCAAACGCTT
CAATGCGAAATGAAATTTGC

Product: alcohol dehydrogenase class III

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MEIKQINSIIKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDAFTLSGSDPEGVFPVVLGHEGAGVVVAV
GEGVLSVKPGDHVIPLYTAECGECEFCRSGKTNLCVSVRDTQGKGLMPDCTTRFSYQGQPIYHYMGCSTFSEYSVVAEVS
LAKINPEANHEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPAKFELAKQFG
ATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALESAHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKG
SAFGGVKGRSELPQMVEDSMKGDIQLEPFVTHTMPLDQINEAFELMHEGKSIRTVIHY

Sequences:

>Translated_378_residues
MEIKQINSIIKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDAFTLSGSDPEGVFPVVLGHEGAGVVVAV
GEGVLSVKPGDHVIPLYTAECGECEFCRSGKTNLCVSVRDTQGKGLMPDCTTRFSYQGQPIYHYMGCSTFSEYSVVAEVS
LAKINPEANHEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPAKFELAKQFG
ATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALESAHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKG
SAFGGVKGRSELPQMVEDSMKGDIQLEPFVTHTMPLDQINEAFELMHEGKSIRTVIHY
>Mature_378_residues
MEIKQINSIIKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDAFTLSGSDPEGVFPVVLGHEGAGVVVAV
GEGVLSVKPGDHVIPLYTAECGECEFCRSGKTNLCVSVRDTQGKGLMPDCTTRFSYQGQPIYHYMGCSTFSEYSVVAEVS
LAKINPEANHEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPAKFELAKQFG
ATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALESAHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKG
SAFGGVKGRSELPQMVEDSMKGDIQLEPFVTHTMPLDQINEAFELMHEGKSIRTVIHY

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=370, Percent_Identity=59.4594594594595, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI34577061, Length=372, Percent_Identity=45.6989247311828, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI71565152, Length=375, Percent_Identity=46.9333333333333, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI4501933, Length=372, Percent_Identity=44.8924731182796, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI4501929, Length=372, Percent_Identity=44.3548387096774, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=44.8648648648649, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=44.8648648648649, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=45.1086956521739, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI4501939, Length=362, Percent_Identity=45.3038674033149, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI156627571, Length=362, Percent_Identity=24.8618784530387, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=89.9728997289973, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI87082125, Length=385, Percent_Identity=25.7142857142857, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787863, Length=356, Percent_Identity=28.6516853932584, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788073, Length=364, Percent_Identity=26.0989010989011, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790045, Length=358, Percent_Identity=26.2569832402235, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI87081918, Length=339, Percent_Identity=28.023598820059, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786825, Length=362, Percent_Identity=24.8618784530387, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786518, Length=369, Percent_Identity=28.7262872628726, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788407, Length=235, Percent_Identity=26.8085106382979, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788075, Length=359, Percent_Identity=25.3481894150418, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI226510992, Length=370, Percent_Identity=27.027027027027, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI25146526, Length=374, Percent_Identity=55.8823529411765, Blast_Score=448, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI71997431, Length=376, Percent_Identity=55.5851063829787, Blast_Score=444, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17565904, Length=338, Percent_Identity=56.508875739645, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71988145, Length=219, Percent_Identity=31.5068493150685, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17562584, Length=360, Percent_Identity=23.6111111111111, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562582, Length=172, Percent_Identity=31.3953488372093, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=380, Percent_Identity=59.4736842105263, Blast_Score=471, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6319621, Length=368, Percent_Identity=27.1739130434783, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323729, Length=378, Percent_Identity=27.7777777777778, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=27.4725274725275, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324486, Length=366, Percent_Identity=27.0491803278689, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323099, Length=381, Percent_Identity=27.2965879265092, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322619, Length=349, Percent_Identity=26.0744985673352, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319955, Length=349, Percent_Identity=26.0744985673352, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI17737895, Length=372, Percent_Identity=61.8279569892473, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI17137530, Length=347, Percent_Identity=25.6484149855908, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI17737897, Length=348, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 40664; Mature: 40664

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKQINSIIKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDAFTLSGSD
CCHHHHHHHHHHCCEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCEEECCEEEECCCC
PEGVFPVVLGHEGAGVVVAVGEGVLSVKPGDHVIPLYTAECGECEFCRSGKTNLCVSVRD
CCCEEEEEECCCCCEEEEEECCCEEEECCCCEEEEEEECCCCCCHHHHCCCCEEEEEEEE
TQGKGLMPDCTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEANHEQVCLLGCGV
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEEEECCCCCCCEEEEEECCH
TTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPAKFELAKQFG
HHCCCHHCCCCEECCCCCEEEEEHHHHHHHHHHCHHHHCCCEEEEEECCCCHHHHHHHCC
ATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALESAHRGWGQSIIIGVAG
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEC
AGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPQMVEDSMKGDIQLEPFVTHTMPLDQIN
CCCCCCCCCEEEEECEEECCCCCCCCCCHHHHHHHHHHHCCCCEEECCEEEECCCHHHHH
EAFELMHEGKSIRTVIHY
HHHHHHHCCCCEEEEECC
>Mature Secondary Structure
MEIKQINSIIKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDAFTLSGSD
CCHHHHHHHHHHCCEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCEEECCEEEECCCC
PEGVFPVVLGHEGAGVVVAVGEGVLSVKPGDHVIPLYTAECGECEFCRSGKTNLCVSVRD
CCCEEEEEECCCCCEEEEEECCCEEEECCCCEEEEEEECCCCCCHHHHCCCCEEEEEEEE
TQGKGLMPDCTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEANHEQVCLLGCGV
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEEEECCCCCCCEEEEEECCH
TTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPAKFELAKQFG
HHCCCHHCCCCEECCCCCEEEEEHHHHHHHHHHCHHHHCCCEEEEEECCCCHHHHHHHCC
ATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALESAHRGWGQSIIIGVAG
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEC
AGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPQMVEDSMKGDIQLEPFVTHTMPLDQIN
CCCCCCCCCEEEEECEEECCCCCCCCCCHHHHHHHHHHHCCCCEEECCEEEECCCHHHHH
EAFELMHEGKSIRTVIHY
HHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]