Click here to switch to the map view.

The map label for this gene is tgt [H]

Identifier: 148825474

GI number: 148825474

Start: 351569

End: 352717

Strand: Direct

Name: tgt [H]

Synonym: CGSHiEE_01855

Alternate gene names: 148825474

Gene position: 351569-352717 (Clockwise)

Preceding gene: 148825473

Following gene: 148825475

Centisome position: 19.39

GC content: 41.86

Gene sequence:

>1149_bases
ATGAAGTATGAATTAGATAAAACAAGCGGCAATGCTCGTCGTGGTCGCTTGGTGTTTGAACGTCCACAGGGCACGTTCAG
TGTAGAAACCCCTGCGTTTATGCCAGTGGGTACTTATGGCACGGTAAAAGGCATGACACCAGAAGAAGTGCGTGCGACAG
GTGCAGAAATTTTACTGGGTAACACCTTCCATTTATGGTTACGTCCAGGGCAAGAAATTATGCGTAAACACGGCGATTTG
CACGACTTTATGCAATGGCATCGCCCGATTTTGACTGACAGTGGCGGTTTCCAAGTATTCAGTTTAGGTAAATTACGTAA
AATTACTGAAGAAGGCGTGAAATTCCAAAACCCAATTAACGGCGAGCGGATCTTCCTTTCGCCTGAAAAATCCATGGAAA
TTCAATATGATTTAGGTTCTGACATCGTGATGATTTTCGATGAATGTACGCCTTATCCAGCAACTTTCGATTATGCGAAA
AAATCTATGGAAATGTCACTTCGTTGGGCAAAACGTAGCCGTGATCGCTTTGATGAATTAGGCAATAAAAATGCACTATT
CGGTATTATTCAAGGCGGCGTATTTGAAGAATTACGTAAAGTATCATTAGAAGGCTTAGTCAATATTGGTTTTGACGGTT
ATGCGGTGGGCGGTTTAGCGGTAGGCGAACCAAAAGAAGATATGCACCGCATTTTAGAATACATCTGCCCACAAATCCCA
GCTGACAAACCACGTTATTTAATGGGCGTGGGTAAACCAGAAGATTTAGTGGAAGGCGTGCGTCGTGGCATTGATATGTT
TGACTGCGTAATGCCAACCCGTAACGCACGTAATGGCCATTTATTTGTGACAGACGGCATTGTCAAAATCCGTAATGCAA
AATATCGTGATGATACCAGCCCGTTAGATCCTGAATGTGATTGCTACACCTGTAAAAACTACACCAAAGCTTATTTATAT
CATTTAGATAAATGCGGTGAAATTTTAGGTGCACGCTTAAATACCATTCACAATTTACGCTATTATCAACGTTTAATGGC
GGAAATTCGTCAAGCGATTGAAGATGATCGTTTTGATGATTTTGTGGTGGAATTCTATGCTCGCATGGGCAAACCTGTTC
CGCCATTACAATTAGCGGATAAATCATAA

Upstream 100 bases:

>100_bases
GCTCAAAGAGCATTGAAAATATGATATCACAAAGCCTGTATTTTTTAACAGGCTTTTTTGTTCAAAAAAATCTTCGAACT
GTTTATTCGTTGGAGCAAAA

Downstream 100 bases:

>100_bases
TTGATGAAAGTGCGGTAGAAAAATAAAACGTTTTTTGACCGCACTTTTTAATAAAGGAAATCTTATGCAAATTCTTGAAC
CTCAACAATTCGCCACATGG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKYELDKTSGNARRGRLVFERPQGTFSVETPAFMPVGTYGTVKGMTPEEVRATGAEILLGNTFHLWLRPGQEIMRKHGDL
HDFMQWHRPILTDSGGFQVFSLGKLRKITEEGVKFQNPINGERIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
KSMEMSLRWAKRSRDRFDELGNKNALFGIIQGGVFEELRKVSLEGLVNIGFDGYAVGGLAVGEPKEDMHRILEYICPQIP
ADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGIVKIRNAKYRDDTSPLDPECDCYTCKNYTKAYLY
HLDKCGEILGARLNTIHNLRYYQRLMAEIRQAIEDDRFDDFVVEFYARMGKPVPPLQLADKS

Sequences:

>Translated_382_residues
MKYELDKTSGNARRGRLVFERPQGTFSVETPAFMPVGTYGTVKGMTPEEVRATGAEILLGNTFHLWLRPGQEIMRKHGDL
HDFMQWHRPILTDSGGFQVFSLGKLRKITEEGVKFQNPINGERIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
KSMEMSLRWAKRSRDRFDELGNKNALFGIIQGGVFEELRKVSLEGLVNIGFDGYAVGGLAVGEPKEDMHRILEYICPQIP
ADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGIVKIRNAKYRDDTSPLDPECDCYTCKNYTKAYLY
HLDKCGEILGARLNTIHNLRYYQRLMAEIRQAIEDDRFDDFVVEFYARMGKPVPPLQLADKS
>Mature_382_residues
MKYELDKTSGNARRGRLVFERPQGTFSVETPAFMPVGTYGTVKGMTPEEVRATGAEILLGNTFHLWLRPGQEIMRKHGDL
HDFMQWHRPILTDSGGFQVFSLGKLRKITEEGVKFQNPINGERIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
KSMEMSLRWAKRSRDRFDELGNKNALFGIIQGGVFEELRKVSLEGLVNIGFDGYAVGGLAVGEPKEDMHRILEYICPQIP
ADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGIVKIRNAKYRDDTSPLDPECDCYTCKNYTKAYLY
HLDKCGEILGARLNTIHNLRYYQRLMAEIRQAIEDDRFDDFVVEFYARMGKPVPPLQLADKS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=374, Percent_Identity=43.5828877005348, Blast_Score=307, Evalue=1e-83,
Organism=Escherichia coli, GI1786607, Length=378, Percent_Identity=81.4814814814815, Blast_Score=655, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=366, Percent_Identity=43.1693989071038, Blast_Score=297, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI25146502, Length=181, Percent_Identity=32.0441988950276, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920510, Length=369, Percent_Identity=43.9024390243902, Blast_Score=311, Evalue=4e-85,
Organism=Drosophila melanogaster, GI21355311, Length=254, Percent_Identity=27.9527559055118, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43642; Mature: 43642

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYELDKTSGNARRGRLVFERPQGTFSVETPAFMPVGTYGTVKGMTPEEVRATGAEILLG
CCEECCCCCCCCCCCCEEEECCCCCEEECCCCEEECCCCCCCCCCCHHHHHHCCCEEEEC
NTFHLWLRPGQEIMRKHGDLHDFMQWHRPILTDSGGFQVFSLGKLRKITEEGVKFQNPIN
CEEEEEECCCHHHHHHCCCHHHHHHHCCCEEECCCCEEEEECHHHHHHHHHCCEECCCCC
GERIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAKKSMEMSLRWAKRSRDRFDEL
CCEEEECCCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GNKNALFGIIQGGVFEELRKVSLEGLVNIGFDGYAVGGLAVGEPKEDMHRILEYICPQIP
CCCCEEEEEECCHHHHHHHHHHHHHHEECCCCCEEECCEECCCCHHHHHHHHHHHCCCCC
ADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGIVKIRNAKYRDDTS
CCCCCEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCC
PLDPECDCYTCKNYTKAYLYHLDKCGEILGARLNTIHNLRYYQRLMAEIRQAIEDDRFDD
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
FVVEFYARMGKPVPPLQLADKS
HHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKYELDKTSGNARRGRLVFERPQGTFSVETPAFMPVGTYGTVKGMTPEEVRATGAEILLG
CCEECCCCCCCCCCCCEEEECCCCCEEECCCCEEECCCCCCCCCCCHHHHHHCCCEEEEC
NTFHLWLRPGQEIMRKHGDLHDFMQWHRPILTDSGGFQVFSLGKLRKITEEGVKFQNPIN
CEEEEEECCCHHHHHHCCCHHHHHHHCCCEEECCCCEEEEECHHHHHHHHHCCEECCCCC
GERIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAKKSMEMSLRWAKRSRDRFDEL
CCEEEECCCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GNKNALFGIIQGGVFEELRKVSLEGLVNIGFDGYAVGGLAVGEPKEDMHRILEYICPQIP
CCCCEEEEEECCHHHHHHHHHHHHHHEECCCCCEEECCEECCCCHHHHHHHHHHHCCCCC
ADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGIVKIRNAKYRDDTS
CCCCCEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCC
PLDPECDCYTCKNYTKAYLYHLDKCGEILGARLNTIHNLRYYQRLMAEIRQAIEDDRFDD
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
FVVEFYARMGKPVPPLQLADKS
HHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]